Assessment of the stability of mephedrone in ante-mortem and post-mortem blood specimens.

Abstract:

AIMS:The aim of this work is to test the stability of mephedrone added to whole blood collected from alive and dead mephedrone free-users and stored at three different temperatures (-20, +4 and +20°C) with and without preservatives up to 6 months, trying to establish the best storage condition in order to reduce possible analyte loss/degradation during the storage period. MATERIALS AND METHODS:Different sources of blood were obtained as follow: 10 samples of blood came from 10 alive mephedrone free-users (mean age 34±15.8 years old) (Group 1), whereas 10 post mortem blood samples were obtained from 10 cadavers, in which the post mortem interval was between 24 and 36h (Group 2). The cause of death in post mortem cases (mean age 45±14.2 years old) was not drug related. Pools of blood were spiked with mephedrone at the concentration of 1mg/L and 1mL aliquots were transferred in 2mL Eppendorf capped tubes with and without preservatives as follow: with ethylenediaminetetraacetic acid (EDTA) 3%; with sodium fluoride/potassium oxalate (NaF/KOx) 1.67%/0.2%, respectively; without preservatives. All samples were stored at three different temperatures: -20°C, 4°C and 20°C and extracted and analyzed in duplicate by GC-MS according to a previously published method by Dickson et al., every other day during the first month and then weekly up to 6 months. RESULTS AND CONCLUSIONS:our study allow us to affirm that -20°C is the best storage temperature for mephedrone stability in ante-mortem and post-mortem blood samples in comparison to the other two tested temperatures (+4 and +20°C), showing higher values in both groups in samples stored with and without preservatives (p<0.0001). The comparison of Group 1 (samples coming from alive subjects) and Group 2 (post-mortem samples) highlights a better stability of mephedrone in Group 1 (p<0.001) at all tested storage conditions. Finally, the analysis of blood specimens stored with and without preservatives in both groups suggests that specimens stored with NaF/KOx maintain mephedrone stability better than those stored with EDTA (p<0.001) and those stored without preservatives (p<0.0001), therefore, we strongly recommend in order to maintain the highest mephedrone stability in blood, to store specimens at -20°C adding NaF/KOx as preservative.

journal_name

Forensic Sci Int

authors

Busardò FP,Kyriakou C,Tittarelli R,Mannocchi G,Pantano F,Santurro A,Zaami S,Baglìo G

doi

10.1016/j.forsciint.2015.07.021

subject

Has Abstract

pub_date

2015-11-01 00:00:00

pages

28-37

eissn

0379-0738

issn

1872-6283

pii

S0379-0738(15)00299-6

journal_volume

256

pub_type

杂志文章
  • Identification of unknown dead bodies by X-ray image comparison of the skull using the X-ray simulation program FoXSIS.

    abstract::The aim of the study was to improve the objectivity of X-ray image comparison for the identification of unknown dead individuals. CT-data were collected for 30 macerated skulls. An already presented computer program which uses CT data to establish virtual X-ray images was used to obtain X-rays with different beam angu...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(00)00452-7

    authors: Riepert T,Ulmcke D,Schweden F,Nafe B

    更新日期:2001-03-01 00:00:00

  • Genetic and chemical components analysis of Papaver setigerum naturalized in Korea.

    abstract::Of the 110 species of genus Papaver, only Papaver somniferum and P. setigerum are controlled poppies in Korea. All poppy samples share similar morphology therefore it is important to check if they contain controlled substances such as morphine and codeine for forensic purpose. Since the alkaloid content of Papaver pla...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2012.08.002

    authors: Choe S,Lee E,Jin GN,Lee YH,Kim SY,Choi H,Chung H,Hwang BY,Kim S

    更新日期:2012-10-10 00:00:00

  • Fingermark detection on non-porous and semi-porous surfaces using NaYF4:Er,Yb up-converter particles.

    abstract::This article describes the first use of an anti-Stokes material, or up-converter, for the development of latent fingermarks on a range of non-porous surfaces. Anti-Stokes materials can absorb long-wavelength light and emit light at a shorter wavelength. This property is unusual in both natural and artificial materials...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2010.09.020

    authors: Ma R,Bullock E,Maynard P,Reedy B,Shimmon R,Lennard C,Roux C,McDonagh A

    更新日期:2011-04-15 00:00:00

  • An epidemiological study on alcohol/drugs related fatal traffic crash cases of deceased drivers in Hong Kong between 1996 and 2000.

    abstract::This study is designed to evaluate the correlation between fatal vehicle crashes (FVC) and consumption of alcohol and/or drugs among drivers. Between 1996 and 2000 in Hong Kong, a total of 197 FVC cases of deceased drivers were investigated. The blood and/or urine samples of the victims were examined for the presence ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2004.08.023

    authors: Cheng JY,Chan DT,Mok VK

    更新日期:2005-10-29 00:00:00

  • Internal validation of two new retrotransposons-based kits (InnoQuant® HY and InnoTyper® 21) at a forensic lab.

    abstract::Obtaining a genetic profile from pieces of evidence collected at a crime scene is the primary objective of forensic laboratories. New procedures, methods, kits, software or equipment must be carefully evaluated and validated before its implementation. The constant development of new methodologies for DNA testing leads...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2017.11.037

    authors: Martins C,Ferreira PM,Carvalho R,Costa SC,Farinha C,Azevedo L,Amorim A,Oliveira M

    更新日期:2018-02-01 00:00:00

  • Undetected drug addict fatalities.

    abstract::A urine screening in fatalities with an unnatural or unknown cause of death (13-40 years of age) was carried out to evaluate the proportion of undetected drug-related cases. Ten out of 160 fatalities without a hint of drug consumption were classified as drug-related according to the definition of the Bundeskriminalamt...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(93)90062-f

    authors: Schulz-Schaeffer W,Elwers W,Schmoldt A

    更新日期:1993-11-01 00:00:00

  • Suicide by drowning: A forensic challenge.

    abstract::In the case of suicidal drowning forensic examination is difficult, particularly with regard to differentiating between suicide, accident, homicide and natural death. Bondage and weighting with objects, putrescence and attendant lesions aggravate interpretation and investigation of postmortal forensic findings. In thi...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2014.04.022

    authors: Todt M,Ast F,Wolff-Maras R,Roesler B,Germerott T

    更新日期:2014-07-01 00:00:00

  • Interaction between apnea, prone sleep position and gliosis in the brainstems of victims of SIDS.

    abstract::Among 27,000 infants studied prospectively to characterize their sleep-wake behavior, 38 infants died suddenly and unexpectedly under 6 months of age. Of these, 26 died from sudden infant death syndrome (SIDS), 5 from congenital cardiac abnormalities, 2 from infected pulmonary dysplasia, 2 from septic shock with multi...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(02)00138-x

    authors: Sawaguchi T,Franco P,Kato I,Shimizu S,Kadhim H,Groswasser J,Sottiaux M,Togari H,Kobayashi M,Nishida H,Sawaguchi A,Kahn A

    更新日期:2002-09-14 00:00:00

  • Stability of explosive residues in methanol/water extracts, on alcohol wipes and on a glass surface.

    abstract::The stability of four target organic explosives [pentaerythritol tetranitrate (PETN), 2,4,6-trinitrotoluene (TNT), hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and triacetone triperoxide (TATP)] and two inorganic anions (chlorate and nitrate) on polyester-based alcohol wipes and in 60% (v/v) methanol/water extracts, ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2013.01.037

    authors: Song-im N,Benson S,Lennard C

    更新日期:2013-03-10 00:00:00

  • Persons found 'not criminally responsible on account of mental disorder': a comparison of British Columbia, Canada and Hunan, China.

    abstract::Current research literature contains very few international, cross-cultural comparison studies of persons adjudicated 'Not Criminally Responsible on Account of Mental Disorder' (NCRMD). This study explores and compares the demographic differences between persons found NCRMD in British Columbia, Canada and Hunan, China...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2005.12.001

    authors: Wang X,Livingston JD,Brink J,Murphy E

    更新日期:2006-12-20 00:00:00

  • 3D GPR in forensics: Finding a clandestine grave in a mountainous environment.

    abstract::In the present work we show a forensic case study carried out in a mountainous environment. Main objective was to locate a clandestine grave which is around 10-20 years old and contains human remains of one individual and a metallic tool, probably a pick. Survey design started with an experimental burial of a pick at ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2010.05.019

    authors: Novo A,Lorenzo H,Rial FI,Solla M

    更新日期:2011-01-30 00:00:00

  • Hair as a source of forensic evidence in murder investigations.

    abstract::Obtaining palynological and other botanical evidence from murder victims is becoming part of routine mortuary protocol in the United Kingdom. Forensic pathologists are often keen to cooperate in the collection of classes of material that have, in the past, been considered to be of little importance in criminal investi...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2006.06.070

    authors: Wiltshire PE

    更新日期:2006-11-22 00:00:00

  • Scanning electron microscope observations of heat-treated human bone.

    abstract::This report describes the heat-induced alterations in human bone tissue observed using scanning electron microscopy and microradiography. Femoral bone samples were taken from persons varying in age from 1 year to 97 years at the time of their death. The bone was heated at selected temperatures in the range 200-1600 de...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(95)01735-2

    authors: Holden JL,Phakey PP,Clement JG

    更新日期:1995-06-30 00:00:00

  • Applicability of Demirjian's method for age estimation in a sample of Italian children with Down syndrome: A case-control retrospective study.

    abstract::Age estimation is widely applied in several clinical and forensic fields. The radiographic evaluation of dental development is one of the most accepted tools for this purpose. Among the different methods proposed, Demirjian's method was the most extensively used and tested in the medical literature revealing that the ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2019.03.015

    authors: Bagattoni S,D'Alessandro G,Gatto MR,Piana G

    更新日期:2019-05-01 00:00:00

  • A modified Raman multidimensional spectroscopic signature of blood to account for the effect of laser power.

    abstract::Raman spectroscopy has been used for the development of a universal method for the identification of traces of all major body fluids. Multidimensional spectroscopic signatures, which account for the intrinsic sample heterogeneity and variations between donors, have been described for blood, semen, saliva, vaginal flui...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2014.04.021

    authors: McLaughlin G,Lednev IK

    更新日期:2014-07-01 00:00:00

  • In situ silver nanoparticles synthesis in agarose film supported on filter paper and its application as highly efficient SERS test stripes.

    abstract::Highly efficient multifunctional agarose SERS test stripes has been fabricated by a convenient facile approach. SERS stripes were found to be highly sensitive, robust, and capable to alleviate the two major drawbacks of filter paper based SERS substrate i.e. the weak adhesion of plasmonic nanoparticles and the diffusi...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2014.01.019

    authors: Raza A,Saha B

    更新日期:2014-04-01 00:00:00

  • Drug addiction in Poland and criminalistic traces.

    abstract::The authors describe the most popular drugs and their substitutes being used in Poland. The paper also presents the problem of developing and examining criminalistic traces in biological and some non-organic materials. ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(90)90150-w

    authors: Kała M,Borkowski T

    更新日期:1990-05-01 00:00:00

  • Quantification of mitochondrial DNA in human blood cells using an automated detection system.

    abstract::The 4977 bp deletion of mitochondrial DNA (mtDNA) accumulates in postmitotic tissues with advancing age. The purpose of our study was to detect and quantify these deletion even in blood cells with a high turnover activity. Whole venous blood, isolated human platelets and peripheral blood mononuclear cells (PBMCs) were...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(00)00249-8

    authors: Meissner C,Mohamed SA,Klueter H,Hamann K,von Wurmb N,Oehmichen M

    更新日期:2000-09-11 00:00:00

  • Determination of cocaine and its metabolites in brain tissue using high-flow solid-phase extraction columns and high-performance liquid chromatography.

    abstract::A new solid-phase extraction procedure for the determination of cocaine and some of its metabolites in brain tissue, using high-flow co-polymeric sorbents is reported as a substantial improvement on our recently reported procedure. The recovery of cocaine, norcocaine and cocaethylene was excellent as was the reproduci...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(92)90197-5

    authors: Moore C,Browne S,Tebbett I,Negrusz A

    更新日期:1992-03-01 00:00:00

  • Immunohistochemical investigation of ubiquitin and myoglobin in the kidney in medicolegal autopsy cases.

    abstract::We examined the immunohistochemical distributions of ubiquitin (Ub) and myoglobin (Mb) in human kidney tissues to assist the pathological assessment of death due to trauma. Medicolegal autopsy cases at our institute (n=138: 0-96 years of age, 105 males and 33 females) were examined. Causes of death were blunt injury (...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2006.10.016

    authors: Ishikawa T,Zhu BL,Li DR,Zhao D,Michiue T,Maeda H

    更新日期:2007-09-13 00:00:00

  • Analysis of the D1S80 (pMCT118) VNTR locus polymorphism in a native Kuwaiti population by the polymerase chain reaction.

    abstract::We have determined the allele and genotype frequencies at the hypervariable locus D1S80 in a native Kuwaiti population using the polymerase chain reaction technique and subsequent high resolution gel electrophoresis. In a sample of 200 individuals, 21 alleles and 57 genotypes were detected. The alleles with 18 and 24 ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(95)01881-6

    authors: al-Nassar KE,Mathew J,Thomas N,Fatania HR

    更新日期:1996-04-02 00:00:00

  • Toxicological screen on gastric aspirates: the Singapore experience.

    abstract::In the early eighties, in response to increasing self-poisoning incidents by pharmaceuticals in Singapore, a comprehensive wide-sweep screen for these drugs in clinical gastric aspirates was devised and implemented by us. The toxicological screen which involved quadruplicate extractions, two in acidic conditions and t...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(92)90158-s

    authors: Chao TC,Lo DS,Gunasegaram R,Tan-Siew WF,Tay MM

    更新日期:1992-05-01 00:00:00

  • Raman spectroscopy and microspectrophotometry of reactive dyes on cotton fibres: analysis and detection limits.

    abstract::A collaborative study on Raman spectroscopy and microspectrophotometry (MSP) was carried out by members of the ENFSI (European Network of Forensic Science Institutes) European Fibres Group (EFG) on different dyed cotton fabrics. The detection limits of the two methods were tested on two cotton sets with a dye concentr...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2012.05.025

    authors: Massonnet G,Buzzini P,Monard F,Jochem G,Fido L,Bell S,Stauber M,Coyle T,Roux C,Hemmings J,Leijenhorst H,Van Zanten Z,Wiggins K,Smith C,Chabli S,Sauneuf T,Rosengarten A,Meile C,Ketterer S,Blumer A

    更新日期:2012-10-10 00:00:00

  • One-step liquid-liquid extraction of cocaine from urine samples for gas chromatographic analysis.

    abstract::An improved technique for cocaine extraction from urine samples for gas chromatographic (GC) analysis is described. Employing a simple liquid-liquid extraction (LLE) of cocaine with a mixture of ethyl ether:isopropanol (9:1) the method presents a mean recovery of 74.49%. Limit of detection (LOD) and limit of quantific...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(02)00124-x

    authors: Farina M,Yonamine M,Silva OA

    更新日期:2002-07-17 00:00:00

  • Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides.

    abstract::Traditionally, sperms are isolated from vaginal cell mixtures by preferential extraction methods. Although these methods work well when there is a reasonable amount of DNA present, they are problematic when there are limited amounts (ca. 250 pg). In particular, the analysis of sperm from microscope slides has proven d...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(03)00267-6

    authors: Elliott K,Hill DS,Lambert C,Burroughes TR,Gill P

    更新日期:2003-10-14 00:00:00

  • Simultaneous determination of drugs of abuse (opiates, cocaine and amphetamine) in human hair by GC/MS and its application to a methadone treatment program.

    abstract::A new method was developed for the simultaneous detection and quantitation of 6-acetyl-morphine (MAM), amphetamine, benzoylecgonine (BZE), cocaine, codeine, dihydrocodeine, EDDP (methadone metabolite), methadone and morphine in hair. The hair samples were washed, cut into 2-cm segments, pulverized, incubated with phos...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(93)90273-d

    authors: Moeller MR,Fey P,Wennig R

    更新日期:1993-12-01 00:00:00

  • Characterisation of chemical component migration in automotive paint by synchrotron infrared imaging.

    abstract::Synchrotron infrared chemical imaging was employed to examine and assess the extent of interlayer component migration within multilayer automotive paint samples, with a particular emphasis on the cross-linking additive melamine. Two dimensional infrared chemical images revealed that melamine consistently diffuses in s...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2013.01.032

    authors: Maric M,van Bronswijk W,Lewis SW,Pitts K,Martin DE

    更新日期:2013-05-10 00:00:00

  • Suicide through doxylamine poisoning.

    abstract::Doxylamine is an antihistamine of the ethanolamine class. It is used primarily as a sleep-inducing agent. Only a few reports can be found in the literature about lethal intoxications with doxylamine, but many with combined intoxications. Doxylamine is, aside from diphenhydramine, the only chemically defined active ing...

    journal_title:Forensic science international

    pub_type: 杂志文章,评审

    doi:10.1016/s0379-0738(00)00393-5

    authors: Bockholdt B,Klug E,Schneider V

    更新日期:2001-06-01 00:00:00

  • A new computer-tomography-based method of sex estimation: Development of Turkish population-specific standards.

    abstract::The identification of victims involved in mass fatality incidents has become an increasingly important issue nowadays, and identification of unknown individuals is an important aspect in criminal cases and Disaster Victim Identification scenarios. Therefore, the sex estimation is one of the most important biological a...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2015.07.015

    authors: Gulhan O,Harrison K,Kiris A

    更新日期:2015-10-01 00:00:00

  • Reprint of: Cling film plastic wrap: An innovation for dead body packaging, preservation and transportation by first responders as a replacement for cadaver body bag in large scale disasters.

    abstract::Cadaver body bags are the conventional method to contain a human body or human remains, which includes the use for storage and transportation of the deceased at any crime scene or disaster scene. During disasters, most often than not, the first responders including the police will be equipped with cadaver body bags to...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2018.04.017

    authors: Khoo LS,Lai PS,Saidin MH,Noor Z,Mahmood MS

    更新日期:2018-07-01 00:00:00