Abstract：
:Obtaining a genetic profile from pieces of evidence collected at a crime scene is the primary objective of forensic laboratories. New procedures, methods, kits, software or equipment must be carefully evaluated and validated before its implementation. The constant development of new methodologies for DNA testing leads to a steady process of validation, which consists of demonstrating that the technology is robust, reproducible, and reliable throughout a defined range of conditions. The present work aims to internally validate two new retrotransposon-based kits (InnoQuant® HY and InnoTyper® 21), under the working conditions of the Laboratório de Polícia Científica da Polícia Judiciária (LPC-PJ). For the internal validation of InnoQuant® HY and InnoTyper® 21 sensitivity, repeatability, reproducibility, and mixture tests and a concordance study between these new kits and those currently in use at LPC-PJ (Quantifiler® Duo and GlobalFiler™) were performed. The results obtained for sensitivity, repeatability, and reproducibility tests demonstrated that both InnoQuant® HY and InnoTyper® 21 are robust, reproducible, and reliable. The results of the concordance studies demonstrate that InnoQuant® HY produced quantification results in nearly 29% more than Quantifiler® Duo (indicating that this new kit is more effective in challenging samples), while the differences observed between InnoTyper® 21 and GlobalFiler™ are not significant. Therefore, the utility of InnoTyper® 21 has been proven, especially by the successful amplification of a greater number of complete genetic profiles (27 vs. 21). The results herein presented allowed the internal validation of both InnoQuant® HY and InnoTyper® 21, and their implementation in the LPC-PJ laboratory routine for the treatment of challenging samples.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Martins C,Ferreira PM,Carvalho R,Costa SC,Farinha C,Azevedo L,Amorim A,Oliveira Mdoi
10.1016/j.forsciint.2017.11.037subject
Has Abstractpub_date
2018-02-01 00:00:00pages
1-8eissn
0379-0738issn
1872-6283pii
S0379-0738(17)30495-4journal_volume
283pub_type
杂志文章abstract：:In this report we describe a first suicide case in Serbia related to ingestion of Taxus baccata leaves. A 30-year old woman was found dead, and the green plant material in a plastic bag was found near her bed. Autopsy revealed dark green needle-like leaves in the stomach, similar to that contained in the plastic bag, ...
journal_title：Forensic science international
pub_type： 杂志文章
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journal_title：Forensic science international
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.02.016
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journal_title：Forensic science international
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.109905
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(98)00062-0
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.11.036
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(88)90152-1
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journal_title：Forensic science international
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.12.024
更新日期：2010-03-20 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：2017-12-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.07.011
更新日期：2014-09-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(90)90243-r
更新日期：1990-02-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2004.09.128
更新日期：2005-11-25 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2020.110196
更新日期：2020-04-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.05.024
更新日期：2013-09-10 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2008.06.014
更新日期：2008-08-25 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(90)90206-e
更新日期：1990-09-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.01.029
更新日期：2019-04-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
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journal_title：Forensic science international
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更新日期：2002-09-14 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：2000-02-14 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章,评审
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更新日期：1998-09-28 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(93)90283-g
更新日期：1993-12-01 00:00:00
abstract：:The current article addresses the psychometric qualities of the German Version of Gudjonsson's Blame Attribution Inventory (GBAI), a self-report scale for measuring attribution of blame for crime. The GBAI was administered to a criminal sample of forensic and criminal inmates (n=107). Findings indicate that the German...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.07.004
更新日期：2007-05-24 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2004.08.002
更新日期：2005-09-10 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.07.023
更新日期：2010-03-20 00:00:00
abstract：:Reported are various techniques to determine the early postmortem period by examining the eye. These include corneal opacity, retinal vessel segmentation, pupil reaction, retinal changes and intraocular pressure. All are subjective, requiring experience to implement the techniques. ...
journal_title：Forensic science international
pub_type： 杂志文章,评审
doi：10.1016/0379-0738(94)90230-5
更新日期：1994-02-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章,评审
doi：10.1016/0379-0738(89)90177-1
更新日期：1989-03-01 00:00:00
