Abstract：
:Amphetamine and related derivatives are widely abused central- and psychostimulants. Detection of certain derivatives, such as methcathinone, by commonly available immunoassay screening techniques is insufficient. Multi-analyte confirmations for amphetamine type stimulants are therefore required, but traditional gas chromatography-mass spectrometry methods necessitate lengthy analytical procedures with prolonged sample turn-around times. A validated rapid GC-MS assay for urinary confirmation of amphetamine, methamphetamine, methcathinone, ephedrine, norephedrine, methylenedioxyamphetamine, methylenedioxymethamphetamine, methylenedioxyethylamphetamine and N-methyl-1-(3,4 methylenedioxyphenyl)-2-butanamine is reported. The method entailed in situ derivatization of urine specimens by extractive acylation with pentafluoropropionic anhydride, followed by rapid chromatography on a microbore capillary column. Analytes were separated in less than 3 min and quantified simultaneously by selected-ion monitoring using stable isotope substituted internal standards. The total instrument cycle-time was 6 min per sample. The limits of detection were between 1.5 ng/mL and 6.25 ng/mL for the various analytes. Intermediate precision and accuracy were in the range of 6.3-13.8% and 90.5-107.3% for the respective analytes at the lower limit of quantitation, and between 5.8-12.6% and 95.4-103.1% for the high control. Long-term storage of methcathinone positive specimens at -20 degrees C proved insufficient stability of this analyte. The proposed assay is precise and accurate for confirmation of amphetamine and derivatives in urine. The complementary approach of extractive-derivatization and fast GC-MS analysis is especially applicable in routine clinical settings where reduced sample turn-around times are required. Further investigation of cathinone as a possible metabolite of methcathinone is warranted, based on results from analyzed authentic urine samples.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Marais AA,Laurens JBdoi
10.1016/j.forsciint.2008.10.021subject
Has Abstractpub_date
2009-01-10 00:00:00pages
78-86issue
1-3eissn
0379-0738issn
1872-6283pii
S0379-0738(08)00426-Xjournal_volume
183pub_type
杂志文章abstract：:One hypothesis for the physical mechanism responsible for backspatter during cranial gunshot wounding is that air is ejected by the collapse of the temporary cavity formed around the bullet path. Using bovine and ovine heads and simulant materials, evidence of this ejection was sought by measuring the velocity of the ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.11.005
更新日期：2015-01-01 00:00:00
abstract：:A pilot study has been performed to evaluate the usefulness of isolated viable rat cardiac myocytes in the evaluation of histochemical oxidoreductase methods for estimating cellular anoxic damage. Data are presented in support of the use of succinate dehydrogenase and beta-hydroxybutyrate dehydrogenase for evaluating ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(80)90207-8
更新日期：1980-11-01 00:00:00
abstract：:A protocol is presented for the collection and analysis of gunshot residues (GSR) from hair. A fine-toothed comb is used for collection of the residues. A small zip-closure bag serves as a container for both sample storage and extraction of the characteristic organic powder additives. The success of this residue recov...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(03)00207-x
更新日期：2003-08-12 00:00:00
abstract：:In the Middle European medico-legal climate, the moral rule 'salus aegroti suprema lex' has been accepted for a long time. In the last few years, under the pressure of fear of accusation of a paternalistic attitude, this postulate has been changed to 'voluntas aegroti suprema lex'. The question stands: Is this valid i...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(94)90399-9
更新日期：1994-12-16 00:00:00
abstract：:Tooth formation is widely used to assess maturity and to predict age. Within clinical dentistry, such information aids in diagnosis and treatment planning. Numerous methods exist that allow either the prediction of age or an assessment of maturation. One widely used method was first described in 1973 by Demirjian et a...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2007.04.228
更新日期：2008-02-25 00:00:00
abstract：:Air weapons are commonly used by civilian populations across the world, particularly by those under 18, and discharges often result in desecration, criminal damage and animal abuse. Online forums and websites provide an accessible resource for civilians to access airgun modification methods proposing to increase muzzl...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.11.004
更新日期：2019-01-01 00:00:00
abstract：:Myxoid heart disease (MHD), more commonly known as mitral valve prolapse, is a very common cardiac abnormality affecting 5-10% of the general population. This article reviews the history, symptoms, physical findings, pathology, associated conditions and complications of this entity. Special emphasis is given to sudden...
journal_title：Forensic science international
pub_type： 杂志文章,评审
doi：10.1016/0379-0738(89)90177-1
更新日期：1989-03-01 00:00:00
abstract：:The key component of the PharmChek sweat patch, the membrane, has been tested for the passage of externally applied materials. Drugs in the uncharged state rapidly penetrated the membrane but charged species were greatly slowed. In basic media, detectable concentrations of cocaine, methamphetamine, and heroin were obs...
journal_title：Forensic science international
pub_type： 临床试验,杂志文章
doi：10.1016/s0379-0738(00)00353-4
更新日期：2001-02-15 00:00:00
abstract：:We describe a method for obtaining specific and reproducible deoxyribonuclease I (DNase I) typing from liquid semen. Isoelectric focusing of the enzymes on polyacrylamide gel (IEF-PAGE, pH 3.5-5) was accomplished using a 0.5-mm thick gel. The separated isozymes were visualized by a new activity staining method, dried ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(92)90043-v
更新日期：1992-11-01 00:00:00
abstract：:Forensic odontologists are involved in all phases of disaster victim identification (DVI). The failure of DVI management to embed odontology teams within all phases of the investigation and to include them in management decisions throughout the operation may lead to delays in the reconciliation process and could possi...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2010.08.013
更新日期：2011-02-25 00:00:00
abstract：:Conclusive evidence was obtained in this study that immunoelectrophoresis could be used in the identification of blood-stains in two particular cases: for diagnosis or approach to diagnosis of the age of a blood-stain, and for diagnosis of the human origin of a blood-stain that had been treated with petroleum products...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(78)90025-7
更新日期：1978-09-01 00:00:00
abstract：:Forensic material of alcoholics was compared with controls with regard to the height, weight, state of nutrition, stature, the ageing process and the presence of traumatic lesions. Surprisingly, it was found that alcoholic women were taller than controls. Alcoholics were significantly more often slightly undernourishe...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(94)01641-h
更新日期：1995-01-21 00:00:00
abstract：:Gamma-hydroxybutyric acid's (GHB's) natural presence in the body has made the interpretation of its levels a challenging task for the forensic toxicologist. This study was designed to measure endogenous GHB levels in antemortem urine and blood samples. The range detected in urine was from 34 to 575 microg/dl and in bl...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(02)00183-4
更新日期：2002-08-28 00:00:00
abstract：:We describe two suicide cases in which old paraquat was ingested. In conjunction with lung involvement a pronounced degeneration was observed in skeletal muscle of one who died on the 14th day after the ingestion. The following sarcoplasmic or endoplasmic reticulum Ca2+ ATPase (SERCA) monoclonal antibodies were used f...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(98)00190-x
更新日期：1999-03-15 00:00:00
abstract：:A total of 48 methamphetamine hydrochloride samples from eight seizures were analyzed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography with a flame ionization detector (GC-FID). Major impurities detected include 1,2-dimethyl-3-phenylaziridine, ephedrine/pseudoephedrine, 1,3-dimethyl-2-phenylna...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2008.09.012
更新日期：2008-11-20 00:00:00
abstract：:The tongue was examined for the presence of haemorrhages in 264 medicolegal autopsy cases. The tongue was sectioned transversely and examined macroscopically and microscopically. Haemorrhages were found in marginal and/or central parts of the tongue in 104 cases. Among them, 28 cases showed haemorrhages in central par...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(82)90127-x
更新日期：1982-11-01 00:00:00
abstract：:The diffusion and trends in use of each substance is a basic information in policy planning of strategies aiming at deterrence of drug abuse or in the organization of the fight against drug trafficking. The actual diffusion of illicit drugs in a population is hardly measurable, but, among the various measures availabl...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.04.018
更新日期：2009-08-10 00:00:00
abstract：:This is the first report in the forensic literature of a combination of fatal digoxin poisoning with endocardial fibroelastosis (EFE). Typical morphological features of EFE as the cause of clinically diagnosed cardiomyopathy were present in the autopsy of a 3-year-old girl, including cardiac hypertrophy and marked thi...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(97)00134-5
更新日期：1997-10-06 00:00:00
abstract：BACKGROUND:Multiple genome-wide association studies (GWAS) and targeted gene sequencing have identified common variants in SCN10A in cases of PR and QRS duration abnormalities, atrial fibrillation and Brugada syndrome. The New York City Office of Chief Medical Examiner has now also identified five SCN10A variants of un...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.05.042
更新日期：2019-08-01 00:00:00
abstract：:Organised criminality is a great concern for national/international security. The demonstration of complex crimes is increasingly dependant on knowledge distributed within law-enforcement agencies and scientific disciplines. This separation of knowledge creates difficulties in reconstructing and prosecuting such crime...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.06.032
更新日期：2007-04-11 00:00:00
abstract：:The RapidHIT® ID is a new instrument which has been designed specifically for non-technical users in non-laboratory environments, for example police custody suites or border control. The development studies presented here were performed using the AmpFℓSTR® NGMSElect™ Express STR kit with single source reference sample...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.12.015
更新日期：2019-02-01 00:00:00
abstract：:The establishment of approaches for the differentiation of the ink entries of seals on paper can provide evidence to authenticate the related documents and can play a key role in judicial expertise. The identification and discrimination method for 38 red ink entries of seals on paper has been investigated using laser ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.12.034
更新日期：2014-03-01 00:00:00
abstract：:The 4977-bp deletion in human mitochondrial DNA (mtDNA) is known to accumulate in various tissues with age. Since this deletion in mtDNA correlates closest with age in muscle tissue, iliopsoas muscle tissue was taken at autopsy from 50 persons aged 24-97 years to determine whether age at death can be estimated based o...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(99)00126-7
更新日期：1999-11-01 00:00:00
abstract：:The identification of forensically important blowflies of the genus Chrysomya (Diptera: Calliphoridae) may be hampered by their close morphological similarities, especially as immatures. In contrast to most previous studies, the utility of a nuclear rather than mitochondrial genetic marker was investigated to solve th...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2008.01.009
更新日期：2008-05-20 00:00:00
abstract：:The post-mortem redistribution of a number of psychiatric drugs was investigated. A portion of liver, the gastric contents and blood collected from heart and femoral sites was obtained from 13 cases and analyzed by liquid chromatography-mass spectrometry. Drugs detected included five selective serotonin reuptake inhib...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.02.013
更新日期：2006-12-20 00:00:00
abstract：:Human hair is among the most common kind of evidence secured at crime scenes. Although DNA analysis through STR-typing is possible in principle, it is not very promising for telogenic hair or single hairs. For the mixed traces frequently found in practice, composed of different hair from an unknown number of individua...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2008.12.018
更新日期：2009-03-10 00:00:00
abstract：:The castor bean plant, Ricinus communis, grows wild throughout many regions of Australia. The seeds of the plant contain the schedule 1 chemical agent ricin, a type II ribosomal inhibiting protein. Currently there are limited analytical techniques that can be applied in analysis of the seeds to establish attribution. ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.11.029
更新日期：2017-01-01 00:00:00
abstract：:Timing of fusion of the spheno-occipital synchondrosis (SOS) is correlated with age. Previous research, however, has demonstrated variation in the timing of closure among different global populations. The present study aims to quantify the timing of SOS fusion in Malaysian individuals as visualised in multi-detector c...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.12.046
更新日期：2018-03-01 00:00:00
abstract：:The effects of absorption time, titer of antiserum or lectin in the absorption stage, and elution temperature on relative antibody or lectin yield in elutes in the absorption-elution procedure were studied in bloodstains and ammoniacal extracts of bloodstains using conventional grouping reagents. In addition, monoclon...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(86)90182-9
更新日期：1986-07-14 00:00:00
abstract：:A 66-year-old patient with terminal heart insufficiency (NYHA IV) received maximum medical therapy, but was also in need of an implantable-cardioverter-defibrillator (ICD). The ICD functioned flawlessly for the whole duration of implantation. It reverted several ventricular tachycardias with anti-tachycardial pacing a...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(01)00633-8
更新日期：2002-02-18 00:00:00
