Development of RapidHIT® ID using NGMSElect™ Express chemistry for the processing of reference samples within the UK criminal justice system.

Abstract:

:The RapidHIT® ID is a new instrument which has been designed specifically for non-technical users in non-laboratory environments, for example police custody suites or border control. The development studies presented here were performed using the AmpFℓSTR® NGMSElect™ Express STR kit with single source reference samples. The system has been successfully optimised for extraction, PCR and electrophoresis. Once optimised studies on sensitivity, in terms of DNA input template, sample repeatability and instrument reproducibility have shown that the instrument is capable of producing robust profiles within an 85min, window, using mock reference buccal samples. This work has been carried out within the validation framework provided by the United Kingdom Forensic Science Regulator and is a key stage in the validation of the RapidHIT® ID for use in custody suites within the UK criminal justice system.

journal_name

Forensic Sci Int

authors

Shackleton D,Gray N,Ives L,Malsom S,Vanhinsbergh D

doi

10.1016/j.forsciint.2018.12.015

subject

Has Abstract

pub_date

2019-02-01 00:00:00

pages

179-188

eissn

0379-0738

issn

1872-6283

pii

S0379-0738(18)31063-6

journal_volume

295

pub_type

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