Abstract:
:The RapidHIT® ID is a new instrument which has been designed specifically for non-technical users in non-laboratory environments, for example police custody suites or border control. The development studies presented here were performed using the AmpFℓSTR® NGMSElect™ Express STR kit with single source reference samples. The system has been successfully optimised for extraction, PCR and electrophoresis. Once optimised studies on sensitivity, in terms of DNA input template, sample repeatability and instrument reproducibility have shown that the instrument is capable of producing robust profiles within an 85min, window, using mock reference buccal samples. This work has been carried out within the validation framework provided by the United Kingdom Forensic Science Regulator and is a key stage in the validation of the RapidHIT® ID for use in custody suites within the UK criminal justice system.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Shackleton D,Gray N,Ives L,Malsom S,Vanhinsbergh Ddoi
10.1016/j.forsciint.2018.12.015subject
Has Abstractpub_date
2019-02-01 00:00:00pages
179-188eissn
0379-0738issn
1872-6283pii
S0379-0738(18)31063-6journal_volume
295pub_type
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