Abstract:
:We extracted the DNA contained in samples of bones and teeth from 10 skeletons excavated from the Gravette site (400-1000 AD, south of France). Ancient DNA was analysed by autosomal short tandem repeats (STRs). The DNA present in these ancient remains appeared very degraded, but nevertheless, better conserved in tooth than in bone samples. Moreover, we showed that the DNA extracted from ancient dental pulp was not exempt from polymerase chain reaction (PCR) inhibitors, which could result from extreme DNA fragmentation. An adapted protocol with a supplementary step of purification removed this inhibition.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Ricaut FX,Keyser-Tracqui C,Crubézy E,Ludes Bdoi
10.1016/j.forsciint.2004.07.001keywords:
subject
Has Abstractpub_date
2005-06-30 00:00:00pages
31-5issue
1eissn
0379-0738issn
1872-6283pii
S0379-0738(04)00420-7journal_volume
151pub_type
历史文章,杂志文章abstract::The differentiation of intoxication courses is one of the most difficult challenges for forensic pathologists and toxicologists. The case of a 52-year-old female inpatient of a psychiatric clinic with multiple medications who died from doxepin intoxication is reported. Concentrations of doxepin metabolites and isomers...
journal_title:Forensic science international
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doi:10.1016/j.forsciint.2012.08.050
更新日期:2013-04-10 00:00:00
abstract::Allele frequencies and population data for 17 Y-STR loci included in a new commercial kit that has recently been available, the AmpFlSTR Y-filer PCR amplification kit (Applied Biosystems), that permits the simultaneous amplification of all the markers included in the actually used European "extended haplotype", DYS19,...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.04.008
更新日期:2007-07-20 00:00:00
abstract::In order to demonstrate the sequence diversity of mitochondrial D-loop DNA in the Taiwanese Han population, we established a database of 155 unrelated individuals. For each individual, the complete 980bp DNA region from the 5' end of HVI to 3' end of HVII segment was sequenced. In these 155 sequence data, 149 differen...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00439-4
更新日期:2001-06-15 00:00:00
abstract::The population of Curaçao, Netherlands Antilles (133,000) shows a very high prevalence of end-stage renal disease (approximately 1 per 1,000). These patients are often treated chronically with haemodialysis. As the drinking water on the island is prepared by distillation of sea water, the haemodialysis fluid used to b...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00159-7
更新日期:2002-08-14 00:00:00
abstract::The spectrophotometric method for the determination of carboxyhemoglobin (HbCO) in blood reported by Fretwurst and Meinecke was modified so as to give the same values of percentage HbCO (HbCO%) as those determined by the oxygen electrode method. Values of HbCO% of nine practical samples determined by both the oxygen e...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(81)90157-2
更新日期:1981-09-01 00:00:00
abstract::The main challenge for the identification and differentiation of lipsticks for forensic purposes is the fact that they have a similar chemical composition - in particular, samples of the same hue. The objective of the presented research was to solve this problem using a nondestructive analytical technique - attenuated...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.09.019
更新日期:2017-11-01 00:00:00
abstract::The use of average curvature measurements for the forensic comparison of curly hairs has been reported, but a method, in which various types of hair form are quantitatively examined and objectively interpreted for hair comparison, has not been reported to date. In the present study, numerical data on hair form from Ja...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00450-4
更新日期:2003-01-28 00:00:00
abstract::Sudden unexplained death (SUD) constitutes a part of the overall sudden death that can not be underestimated. Over the last years, genetic testing on SUD has revealed that inherited channelopathies might play important roles in the pathophysiology of this disease. Ryanodine receptor type-2 (RYR2) is a kind of ion chan...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2016.12.005
更新日期:2017-01-01 00:00:00
abstract::A number of biological and chemical tests have been developed over the years to determine whether a person was drowned. This study focuses on the potential of a microbiological test for detecting common bacterial markers of water faecal pollution such as faecal coliforms (FC) and faecal streptococci (FS) as possible i...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2008.09.004
更新日期:2008-11-20 00:00:00
abstract::Etizolam is a drug from the thienotriazoldiazepine class, widely prescribed as anxiolytic due to its apparently secure toxicological profile. Nevertheless, some recent cases of etizolam dependence, intoxications and fatalities associated to its abuse have been reported in the international literature. For this reason,...
journal_title:Forensic science international
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.forsciint.2019.05.018
更新日期:2019-08-01 00:00:00
abstract::In a study of subjects in drug rehabilitation programs, cocaine and cocaine metabolite levels were determined in the hair of 75 subjects who had produced cocaine-positive urine results. The hair was analyzed after being washed with the 3.75 h wash procedure developed by this laboratory. In addition, results of testing...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2004.04.033
更新日期:2004-10-29 00:00:00
abstract::Analysis of cannabis has gained new importance worldwide, mainly for quality control within the legalized recreational and medical cannabis industry, but also for forensic differentiation between drug-type cannabis and legal products such as fiber hemp and CBD-rich/THC-poor cannabis. We herein present an HPLC-DAD meth...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.03.046
更新日期:2019-06-01 00:00:00
abstract::The article presents research on the methods of crash velocity determination based on the Compact car class. The database used in the research is provided by the NHTSA (National Highway Traffic Safety Administration) and includes numerous frontal crash tests, which allowed the determination of the mathematical model p...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.11.023
更新日期:2019-02-01 00:00:00
abstract::A 26-year-old male was found naked and excited in the backyard of his neighbor's house. He was carried to a nearby hospital, and returned home with his family, but took a sudden turn for the worse and died. In a judicial autopsy, the ethanol concentration of blood was found to be 0.58 milligrams, and methamphetamine (...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(93)90209-s
更新日期:1993-12-01 00:00:00
abstract::Human teeth are biological structures that resist extreme conditions thus becoming a useful source of DNA for human forensic identification purposes. When it is possible, forensic prefer only non-damaged teeth whereas those with cavities are usually rejected to avoid both external and internal bacterial contamination....
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.08.021
更新日期:2015-12-01 00:00:00
abstract:BACKGROUND:Internationally, urine on-site testing has been used for detecting drivers under the influence of drugs (DUID) but more and more countries, such as Belgium, are switching to oral fluid screening. OBJECTIVE:To compare the previous (published in 1999) and current (published 2009) enforcement procedures of DUI...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.01.034
更新日期:2015-04-01 00:00:00
abstract::The hair samples of 15 grizzled patients with a permanent medical treatment by amitriptyline, carbamazepine, chlorprothixene, diclofenac, doxepine, indomethacine, maprotiline or metoclopramide, or with a chronic heroin and cocaine abuse were separated into white and pigmented fibers and both fractions were independent...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(96)02048-8
更新日期:1997-01-17 00:00:00
abstract::The hairy maggot blow fly, Chrysomya rufifacies (Diptera: Calliphoridae), is a forensically important fly often encountered on human and other vertebrate remains in temperate and tropic regions throughout the world including Australia, Asia, Central America and North America. C. rufifacies was reared under controlled ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.09.023
更新日期:2014-12-01 00:00:00
abstract::A sandwich ELISA for human prostate-specific antigen (PSA) is described. Optimal assay conditions, resulting in a sensitive assay with a low background, are presented. The method uses a hyperimmune antiserum produced in the New Zealand white rabbit, against human semen PSA. The IgG fraction of the antiserum was conjug...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(91)90141-5
更新日期:1991-07-01 00:00:00
abstract::The Forensic Anthropology Service of the Medical Examiner's Office of Porto Alegre was created in September 1997 to examine human skeletons, establish identification and identify lesions that may explain death. From September 1997 to December 2006, 344 skeletons were examined (mean: 37 examinations/year), and 322 were...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2008.05.004
更新日期:2008-08-06 00:00:00
abstract:UNLABELLED:It is frequently difficult to prove the vitality in cases of fatal hanging. Using impulse cytophotometry, Müller et al. (Krim. Forens. Wiss. 44, 1981) reported on possibly vital alterations of the DNA content in five strangulation marks (hypodiploidy). The objective of the present study was to perform a re-e...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(97)00069-8
更新日期:1997-09-19 00:00:00
abstract::For PCR-based genotyping using polymorphic microsatellite markers, DNA from decomposed postmortem human tissues was fractionated into six groups according to molecular size. The minimum required amounts of this degraded DNA, for detecting alleles at five microsatellite loci (ACTBP2, CMAG, HUMTH01, CYP19, and LPL) and ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(97)00129-1
更新日期:1997-11-10 00:00:00
abstract::A new solid-phase extraction procedure for the determination of cocaine and some of its metabolites in brain tissue, using high-flow co-polymeric sorbents is reported as a substantial improvement on our recently reported procedure. The recovery of cocaine, norcocaine and cocaethylene was excellent as was the reproduci...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(92)90197-5
更新日期:1992-03-01 00:00:00
abstract::One hundred and seventy nine cases of acute poisoning constituting 1.1% of all admissions to the medical unit over 3 years were analysed. Eighty-three per cent of the patients were under 30 years of age and 72% were males. The common agents were: OP insecticides in 51%, kerosene oil in 12%, rat poison in 8% and drugs ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(88)90221-6
更新日期:1988-01-01 00:00:00
abstract::Human hair is among the most common kind of evidence secured at crime scenes. Although DNA analysis through STR-typing is possible in principle, it is not very promising for telogenic hair or single hairs. For the mixed traces frequently found in practice, composed of different hair from an unknown number of individua...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2008.12.018
更新日期:2009-03-10 00:00:00
abstract::The AmpFlSTR Identifiler kit has recently been accepted for use in DNA databasing of forensic samples in the FBI's National DNA Index System. In the present study, we used this kit to analyze the allele distribution of 15 short tandem repeat markers (STR) in individuals living in Caracas city, Venezuela. The allele fr...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(03)00259-7
更新日期:2003-09-09 00:00:00
abstract::At the beginning of 2015, sixty-two capsules containing red-brown crystals seized in a historical city in Brazil were sent to this forensic laboratory for drug testing analysis. The material was identified as being Brephedrone, a new psychoactive substance and a bromine synthetic cathinone that is related to serotonin...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.04.007
更新日期:2017-06-01 00:00:00
abstract::We examined the distribution of drugs in a 49-year-old brain-dead man. Our objective was to determine the possibility of diagnosing how and at what point the patient became brain dead. The presence of mepivacaine, pentazocine, lidocaine and thiamylal in various tissues, including seven regions of the brain were confir...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(97)00155-2
更新日期:1997-11-10 00:00:00
abstract::The ability to recover trace DNA from fired cartridge cases can help establish important leads regarding the handler of the ammunition. Over recent years, several DNA recovery techniques for fired ammunition have been published. Three techniques of significant interest include tape lifting, direct PCR, and vacuum filt...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110507
更新日期:2020-12-01 00:00:00
abstract:INTRODUCTION:The use of methadone in opioid maintenance treatment (OMT) is potentially associated with a number of adverse effects and the risk of fatal toxicity. Increased methadone availability may lead to an increase in methadone-related deaths. We have investigated methadone-related deaths in Norway over the period...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.11.010
更新日期:2013-01-10 00:00:00