STR-genotyping from human medieval tooth and bone samples.

Abstract:

:We extracted the DNA contained in samples of bones and teeth from 10 skeletons excavated from the Gravette site (400-1000 AD, south of France). Ancient DNA was analysed by autosomal short tandem repeats (STRs). The DNA present in these ancient remains appeared very degraded, but nevertheless, better conserved in tooth than in bone samples. Moreover, we showed that the DNA extracted from ancient dental pulp was not exempt from polymerase chain reaction (PCR) inhibitors, which could result from extreme DNA fragmentation. An adapted protocol with a supplementary step of purification removed this inhibition.

journal_name

Forensic Sci Int

authors

Ricaut FX,Keyser-Tracqui C,Crubézy E,Ludes B

doi

10.1016/j.forsciint.2004.07.001

keywords:

subject

Has Abstract

pub_date

2005-06-30 00:00:00

pages

31-5

issue

1

eissn

0379-0738

issn

1872-6283

pii

S0379-0738(04)00420-7

journal_volume

151

pub_type

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