Abstract:
:For PCR-based genotyping using polymorphic microsatellite markers, DNA from decomposed postmortem human tissues was fractionated into six groups according to molecular size. The minimum required amounts of this degraded DNA, for detecting alleles at five microsatellite loci (ACTBP2, CMAG, HUMTH01, CYP19, and LPL) and one minisatellite locus (MCT118) were investigated respectively. The allele patterns were detected by electrophoresis of the PCR products on a 6%-denaturing polyacrylamide gel following silver staining. The detection of alleles for the loci with large allele size required more template DNA with higher molecular size than for that with small allele size. Amounts from 0.3 ng to 5 ng were needed for allele detection on genomic DNA from fresh blood. When the decomposed DNA mixture was used as the template, approximately ten times the amount of genomic DNA was required to detect alleles at the three loci of LPL, CYP19 and HUMTH01, while 24 to 67 times was required for the loci, CMAG, ACTBP2 and MCT118. It was demonstrated that a minimum molecular, size and amount of template DNA was needed for amplifying alleles of the six loci, and degraded DNA less than minimum size in the samples would prevent the detection of the loci which have large allele size.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Takahashi M,Kato Y,Mukoyama H,Kanaya H,Kamiyama Sdoi
10.1016/s0379-0738(97)00129-1subject
Has Abstractpub_date
1997-11-10 00:00:00pages
1-9issue
1-2eissn
0379-0738issn
1872-6283pii
S0379-0738(97)00129-1journal_volume
90pub_type
杂志文章abstract::The aim of this study was to characterize the concentrations of total and specific IgE as well as mast cell-derived tryptase in postmortem serum from femoral blood and evaluate the usefulness of their determination for diagnostic purposes in cases of fatal asthma attacks. 36 autopsy cases were retrospectively selected...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2016.11.001
更新日期:2016-12-01 00:00:00
abstract::We extracted the DNA contained in samples of bones and teeth from 10 skeletons excavated from the Gravette site (400-1000 AD, south of France). Ancient DNA was analysed by autosomal short tandem repeats (STRs). The DNA present in these ancient remains appeared very degraded, but nevertheless, better conserved in tooth...
journal_title:Forensic science international
pub_type: 历史文章,杂志文章
doi:10.1016/j.forsciint.2004.07.001
更新日期:2005-06-30 00:00:00
abstract::The dead body of a 44-year-old woman, previously known for depression and alcoholism, has been discovered at her place of residence by her husband. A forensic autopsy has been carried out. The results indicated unspecific histological lesions (alveolar oedema, liver steatosis and interstitial nephritis) but did not re...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2008.03.003
更新日期:2008-07-04 00:00:00
abstract::Foreign objects as acquired characteristics in footwear examination have a great value in declaring a proposed identification. However, they do not have the same significance in excluding any footwear because they may disappear for many reasons as time passes by. The aim of this study was to establish the value of for...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.109949
更新日期:2019-11-01 00:00:00
abstract::Chemical evaluation of gunshot residues (GSR) produced by non-toxic lead-free ammunition (NTA) has been a challenge to forensic analyses. Our group developed some luminescent markers specific to the detection of GSR. Here, we evaluated the performance of selected markers in experiments that mimic forensic context and/...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.09.001
更新日期:2014-11-01 00:00:00
abstract::We investigated 40 patients who had died following open heart surgery between January 1990 and May 1992. Between this time, 703 open heart procedures were carried out at the University Medical School of Debrecen, 2nd Department of Surgery's Cardiac Surgery Unit with a mortality rate of 8.3%. We studied each individual...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(96)01896-8
更新日期:1996-05-31 00:00:00
abstract::Since 1970, traumatomechanics has been a focal point in research at the Institute for Legal Medicine and Traffic Medicine in Heidelberg. Here, the main topics are the understanding of the interrelation between mechanical strain and the resulting degree of injury; at the forefront of all interest is the determination o...
journal_title:Forensic science international
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.forsciint.2004.04.082
更新日期:2004-09-10 00:00:00
abstract::In the process of questioned document examination, the discrimination of inkjet printing inks is becoming more necessary due to increasingly frequent counterfeiting of documents printed by inkjet printers. Therefore, a method based on micellar electrophoretic capillary chromatography (MECC) has been developed and appl...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2011.05.017
更新日期:2011-10-10 00:00:00
abstract:OBJECTIVE:Age estimation in adults is an important issue in forensic science. This study aimed to estimate the chronological age of Iranians by means of pulp/tooth area ratio (AR) of canines in digital panoramic radiographs. MATERIALS AND METHODS:The sample consisted of panoramic radiographs of 271 male and female sub...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.01.016
更新日期:2018-04-01 00:00:00
abstract::The area of the regional unit of Evros in northern Greece is considered the main gateway to the East and marks the end of the European territory and the beginning of the Asian continent. The natural boundary of the Evros River has been the last major obstacle to overcome before entering the Schengen area for hundreds ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.109875
更新日期:2019-09-01 00:00:00
abstract::Benzodiazepines are one of the most widely prescribed drugs for the treatment of a wide spectrum of clinical disorders. They are used as anticonvulsants, anxiolytics, hypnotics or muscle relaxants with different duration of action. In this paper, a simple and sensitive method for the determination of benzodiazepines i...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00226-7
更新日期:2000-09-11 00:00:00
abstract::The aims of this study were: first, to determine the accuracy of the Cameriere method for assessing chronological age in children based on the relationship between age and measurement of open apices in teeth and, second, to compare the accuracy of this method with the widely used Demirjian et al. method and with the m...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2007.09.001
更新日期:2008-04-07 00:00:00
abstract::Human teeth are biological structures that resist extreme conditions thus becoming a useful source of DNA for human forensic identification purposes. When it is possible, forensic prefer only non-damaged teeth whereas those with cavities are usually rejected to avoid both external and internal bacterial contamination....
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.08.021
更新日期:2015-12-01 00:00:00
abstract::A 39-year-old woman was found dead at home. She was single, foreign national, chronic alcoholic, and had given shelter to a compatriot for three days. This man found the deceased in her bed, after a night of heavy drinking. The emergency medical team observed numerous recent blue ecchymoses of the upper limbs. Because...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2011.08.004
更新日期:2012-01-10 00:00:00
abstract::The response to wounds until healing requires the activity of many cell types coordinate in space and time, so that the types of cells in a wound and their localization may be of help to date lesions with respect to death, which would be useful in forensic pathology. Cells reacting to injury include dendritic cells; t...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.08.024
更新日期:2014-11-01 00:00:00
abstract::The authors describe a technique for the gross examination of postmortem eyes of children who are suspected to have been the victims of deliberate trauma. By removing the anterior segment (cornea, iris, lens and pars plicata of the ciliary body) en bloc by a coronal incision through the pars plana just anterior to the...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(92)90149-q
更新日期:1992-09-01 00:00:00
abstract::The presence of Ethyl glucuronide (EtG) in hair provides a strong indication of ethanol consumption and its investigation is of interest in both clinical and forensic contexts because of the wide window of detection. However, due to the possibility of false negative results in cases of small ethanol intake or excessiv...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.110071
更新日期:2020-01-01 00:00:00
abstract::During a 3-year period (August 1st, 1985 to July 31st, 1988) a systematic investigation of medico-legal autopsy cases with regard to the presence of antibodies for HIV-virus was carried out at the Department of Forensic Medicine in Stockholm, Sweden. Prior to autopsy, blood samples were taken from femoral or subclavia...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(89)90220-x
更新日期:1989-06-01 00:00:00
abstract::There are numerous types of fluorescent fingermark powders or reagents used with the visualization of latent fingermarks deposited on multicolored substrate surfaces that can present a contrast problem if developed with regular fingermark powders. The developed fingermarks can show bright fluorescence upon exposure to...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2008.10.008
更新日期:2009-01-10 00:00:00
abstract::Tasmanian opium accounts for 25% of the world's legal supply of opium straw, and in 1998-99 sufficient numbers of flower pods (66,013) to manufacture ca 500 kg of heroin were stolen. Whilst the heroin signature program has been developed to determine the origin of heroin from other key producers, no such signature cur...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2007.07.002
更新日期:2008-03-05 00:00:00
abstract::One of the most important factors for calculating the accumulated degree days (ADD), which is used to estimate the postmortem interval (PMI) is the accurate estimation of the scene temperature after death. As the estimation method used up to now, they applied to the linear regression analysis using the temperature of ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110196
更新日期:2020-04-01 00:00:00
abstract::This paper describes a sensitive UPLC-MS/MS method for quantification of methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in single empty puparial case of Lucilia sericata. Larvae were reared on substrates spiked with different concentrations of methadone (0-4 μg/g). Methadone was quantified in p...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2011.01.020
更新日期:2011-06-15 00:00:00
abstract::Of the fifty-seven cases of cot death studied two-thirds were younger than 3 months, which is also the peak age of infantile hypoglycaemia. Findings from routine necropsy and histology were scarce; in eleven cases they could be regarded as potentially fatal. About half of the infants had had a mild virus-type infectio...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(80)90205-4
更新日期:1980-11-01 00:00:00
abstract::Reddish discoloration of exposed skin areas, called frost erythema, is an important criterion for the diagnosis of fatal hypothermia. In the present study, we used immunohistochemistry in a prospective trial to show that on the molecular level, the correlate of frost erythema is hemoglobin without hemorrhage. Furtherm...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2005.05.029
更新日期:2006-05-10 00:00:00
abstract::Micro-computed tomography (μCT) provides micrometric 3D images and has been used in forensic studies for anthropology pubis measurement or insect description for post mortem interval estimation. Studies have suggested using registration, a superimposing images method between a reference and a target. This technique av...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.02.005
更新日期:2017-04-01 00:00:00
abstract::The UK Government decision to close its Forensic Science Service (FSS) in 2010 left the criminal justice system in England and Wales bereft of impartial, high-level scientific support. The private sector was entrusted to fill the gap and to ensure that all results were accurate, timely, fit for purpose, easy to interp...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.06.038
更新日期:2018-09-01 00:00:00
abstract::This study explores the applicability of micro-Raman spectroscopy as a non-destructive technique for the analysis of color toner printed counterfeits. The main aim of the research paper was to find out whether Raman spectroscopy is a suitable method for establishing the connection between different specimens of counte...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.10.007
更新日期:2012-11-30 00:00:00
abstract::Gasoline was added to moldy samples of unused building materials. The unused samples were allowed to sit at room temperature for 2, 4, 7, and 14 days. Each set of samples was extracted using passive headspace concentration and analyzed using gas chromatography-mass spectrometry. Microbial degradation of the gasoline p...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.08.006
更新日期:2013-10-10 00:00:00
abstract::Traces of the improvised explosive urea nitrate can be characterized by a sensitive colorimetric reaction with p-dimethylaminocinnamalaldehyde (p-DMAC, UN-1 reagent). As recently shown, the dark red product has a structure of a protonated Schiff base. The unprotonated free base, previously postulated in the literature...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2010.10.017
更新日期:2011-05-20 00:00:00
abstract:BACKGROUND:The mandibular bone is an important part of the forensic facial reconstruction and it has the possibility of getting lost in skeletonized remains; for this reason, it is necessary to facilitate the identification process simulating the mandibular position only through craniomaxillary measures, for this task,...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.12.025
更新日期:2016-04-01 00:00:00