Abstract:
:To investigate the DNA mutation rate and pattern in the hypervariable short tandem repeat (STR) locus D12S391 and in the locus D8S1132, samples from an eastern German population (Dresden area) were analysed. A duplex PCR was applied, using short amplification products for D12S391 (129-177bp) and a modified reverse primer for D8S1132 (127-182bp). The sequences of some rare and new variant alleles are described. At the locus D12S391, 13 regular and six incomplete alleles with different lengths were found, exhibiting several sequence structures. Two isolated father/child mismatches were observed in a total of 648 meioses. Novel alleles 13.1, 14.1 and 27 were discovered at the locus D8S1132. Three parent/child mismatches were found in a total of 672 meioses.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Hering S,Müller Edoi
10.1016/s0379-0738(01)00596-5keywords:
subject
Has Abstractpub_date
2001-12-27 00:00:00pages
187-91issue
2-3eissn
0379-0738issn
1872-6283pii
S0379073801005965journal_volume
124pub_type
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