New alleles and mutational events in D12S391 and D8S1132: sequence data from an eastern German population.

Abstract:

:To investigate the DNA mutation rate and pattern in the hypervariable short tandem repeat (STR) locus D12S391 and in the locus D8S1132, samples from an eastern German population (Dresden area) were analysed. A duplex PCR was applied, using short amplification products for D12S391 (129-177bp) and a modified reverse primer for D8S1132 (127-182bp). The sequences of some rare and new variant alleles are described. At the locus D12S391, 13 regular and six incomplete alleles with different lengths were found, exhibiting several sequence structures. Two isolated father/child mismatches were observed in a total of 648 meioses. Novel alleles 13.1, 14.1 and 27 were discovered at the locus D8S1132. Three parent/child mismatches were found in a total of 672 meioses.

journal_name

Forensic Sci Int

authors

Hering S,Müller E

doi

10.1016/s0379-0738(01)00596-5

keywords:

subject

Has Abstract

pub_date

2001-12-27 00:00:00

pages

187-91

issue

2-3

eissn

0379-0738

issn

1872-6283

pii

S0379073801005965

journal_volume

124

pub_type

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