Abstract：
:Hypertension is a growing medical concern in the United States. With the number of Americans suffering from hypertension increasing, the use of antihypertensives such as beta-blockers is increasing as well. In fact, three beta-blockers - atenolol, metoprolol and propranolol - were among the 200 most prescribed medications in the United States in 2003. Pilots that successfully manage their hypertension can remain certified to fly. The Federal Aviation Administration currently designates approximately 8% of active pilots as "hypertensive with medication". The Civil Aerospace Medical Institute (CAMI) performs toxicological evaluation on victims of fatal aviation accidents. At CAMI beta-blockers are analyzed using gas chromatography with mass spectrometric detection. We have, however, recently developed a liquid chromatography with mass spectrometric detection (LC/MS) method for the simultaneous quantitation of three commonly prescribed beta-blockers, atenolol, metoprolol and propranolol. One advantage of our LC/MS method is the specificity provided by an ion trap MS. Utilizing an ion trap MS, we were able to conduct MS/MS and MS/MS/MS on each analyte. This method also eliminates the time-consuming and costly derivitization step necessary during GC/MS analysis. Additionally, by utilizing this novel method, any concerns about beta-blocker metabolite and/or sample matrix interference are eliminated. The limits of detection for this method ranged from 0.39 to 0.78 ng/mL and the linear dynamic range was generally 1.6-3200 ng/mL. The extraction efficiencies for each analyte ranged from 58% to 82%. This method was successfully applied to postmortem fluid and tissue specimens obtained from victims of three separate aviation accidents.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Johnson RD,Lewis RJdoi
10.1016/j.forsciint.2005.01.001keywords:
subject
Has Abstractpub_date
2006-01-27 00:00:00pages
106-17issue
2-3eissn
0379-0738issn
1872-6283pii
S0379-0738(05)00006-Xjournal_volume
156pub_type
杂志文章abstract：:Changes related to chronological age are seen in both hard and soft tissue. A number of methods for age estimation have been proposed which can be classified in four categories, namely, clinical, radiological, histological and chemical analysis. In forensic odontology, age estimation based on tooth development is univ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.09.020
更新日期：2014-01-01 00:00:00
abstract：:Following a recent meeting by the ENFSI and EDNAP groups on the 4-5 April, 2005, in Glasgow, UK, it was unanimously agreed that the process of standardization within Europe should take account of recent work that unequivocally demonstrated that chance of obtaining a result from a degraded sample was increased when sma...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.05.036
更新日期：2006-01-27 00:00:00
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journal_title：Forensic science international
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doi：10.1016/0379-0738(81)90186-9
更新日期：1981-01-01 00:00:00
abstract：BACKGROUND:The study of post mortem vitreous magnesium (Mg) is less common than sodium (Na), chloride (Cl) and potassium (K) in the forensic literature. There is no accepted normal range for post mortem vitreous Mg and the relationship between post mortem vitreous Mg levels and post mortem interval (PMI), other electro...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.12.038
更新日期：2018-03-01 00:00:00
abstract：:A classification system is described for drugs using thin-layer chromatography (TLC), gas-liquid chromatography (GLC) and ultraviolet (UV) spectrophotometry. The TLC classifications are based on division of the plate into zones relative to a set of mixed drug standards. The GLC classification is based on Kovats' reten...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(79)90265-2
更新日期：1979-01-01 00:00:00
abstract：:The aim of this study was to characterize the concentrations of total and specific IgE as well as mast cell-derived tryptase in postmortem serum from femoral blood and evaluate the usefulness of their determination for diagnostic purposes in cases of fatal asthma attacks. 36 autopsy cases were retrospectively selected...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.11.001
更新日期：2016-12-01 00:00:00
abstract：:DNA molecular techniques were used in a forensic investigation involving the poaching of wildlife in a national park of Italy. A poacher, after having snared a wild boar (Sus scrofa) sow, knifed it to death. The animal was retrieved by conservation officers at the scene before the poacher could remove the carcass. Sub...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.04.032
更新日期：2005-10-29 00:00:00
abstract：:An increased number of police enquiries involving human remains buried with lime have demonstrated the need for more research into the effect of different types of lime on cadaver decomposition and its micro-environment. This study follows previous studies by the authors who have investigated the effects of lime on th...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.12.046
更新日期：2014-05-01 00:00:00
abstract：:Clinical and research-based tests in molecular biology require a substantial amount of DNA and RNA, unfortunately, a considerable number of cells is needed for this amount of sample. Blood is one of the best and easiest source of cells, but is not used due to its invasive drawing methods and the needed volume. Another...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.109931
更新日期：2019-10-01 00:00:00
abstract：:To improve the possibilities to delimitate the time of death after longer laytime, it was examined if this is possible by immunohistochemical insulin detection. The results show that in our examination material, the pancreatic beta-cells of up to 12-day-old corpses produce a positive immunoreaction towards insulin in ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(99)00124-3
更新日期：1999-11-08 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2004.02.010
更新日期：2004-06-30 00:00:00
abstract：:The article is devoted to the comparative analysis of effectiveness of the most widely used methods for medico-legal examination of the hyoid bone and laryngeal cartilages, i.e. palpation, radiography and complete preparation. A consecutive study of 137 cases of hanging (including 20 cases of roentgenological analysis...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(97)00101-1
更新日期：1997-08-22 00:00:00
abstract：:Urine morphine levels after the consumption of poppy seeds were measured in two separate trials. Maximum levels of approximately 18 micrograms/ml were found using RIA, EMIT-ST and GC methodologies. Positive immunoassay results were seen up to 60 h post-ingestion. Several different lots of seeds from various sources we...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(85)90173-2
更新日期：1985-02-01 00:00:00
abstract：:In Japan, recent trends have seen wild silk preferred over cultivated silk because of its texture. Some cases of fraud have occurred where cultivated silk garments are sold as wild silk. Samples from these cases, morphological observation using light microscope and polarized microscope have been conducted in forensic ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.05.004
更新日期：2013-09-10 00:00:00
abstract：:The Mombello Psychiatric Hospital in Limbiate, near Milan, replaced the old Senavra Hospital as the Psychiatric Hospital for the Province of Milan in the 19th century. During the early 20th century, bodies of several Mombello patients were dissected and preserved by Giuseppe Paravicini, an anatomist who operated withi...
journal_title：Forensic science international
pub_type： 历史文章,杂志文章
doi：10.1016/j.forsciint.2016.11.011
更新日期：2017-01-01 00:00:00
abstract：:In forensics and archaeology, it is important to distinguish human from animal remains and to identify animal species from fragmentary bones and bloodstains. We report blind tests in which a protein radioimmunoassay (pRIA) was used to identify the species of six bone fragments lacking morphological specificity and 43 ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.08.007
更新日期：2006-06-02 00:00:00
abstract：:The aim of the present study was to investigate stress gene expression in cultured primary fibroblasts established from Achilles tendons collected during autopsies from sudden infant death syndrome (SIDS) cases, and age-matched controls (infants dying in a traumatic event). Expression of 4 stress responsive genes, HSP...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.06.003
更新日期：2013-10-10 00:00:00
abstract：:The timely identification of vehicles involved in an accident, such as a hit-and-run situation, bears great importance in forensics. To this end, procedures have been defined for analyzing car paint samples that combine techniques such as visual analysis and Fourier transform infrared spectroscopy. This work proposes ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.12.040
更新日期：2016-02-01 00:00:00
abstract：:The judicial system calls upon expert witnesses to testify in court when complex or specialized knowledge, beyond that of the lay person, is needed to interpret the evidence. Previous research has indicated that particular traits of the expert witness can affect their credibility in the eyes of the jury, however most ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.07.030
更新日期：2018-10-01 00:00:00
abstract：:Pyrethroids, organic compounds similar to natural pyrethrums, constitute the majority of insecticides. Pyrethroids are widely used around the world owing to their excellent selective toxicity to certain insects. In addition, they are easily found in daily life, accounting for most household pesticides. Owing to the ea...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.06.004
更新日期：2019-09-01 00:00:00
abstract：:Besides alcohol and drugs of abuse, several popular foods contain potentially toxic substances and cases of intoxication after consumption of these foods attract notice of forensic toxicology. This is also true for the case of a 34-year-old woman who was suspected to have suffered lethal acute intoxication from eating...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.12.018
更新日期：2010-04-15 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(78)90025-7
更新日期：1978-09-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(01)00513-8
更新日期：2001-11-15 00:00:00
abstract：:The reference database of highly informative Y-chromosomal short tandem repeat (STR) haplotypes (YHRD), available online at http://ystr.charite.de, represents the largest collection of male-specific genetic profiles currently available for European populations. By September 2000, YHRD contained 4688 9-locus (so-called...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(00)00478-3
更新日期：2001-05-15 00:00:00
abstract：:The AmpFlSTR Identifiler kit has recently been accepted for use in DNA databasing of forensic samples in the FBI's National DNA Index System. In the present study, we used this kit to analyze the allele distribution of 15 short tandem repeat markers (STR) in individuals living in Caracas city, Venezuela. The allele fr...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(03)00259-7
更新日期：2003-09-09 00:00:00
abstract：:When someone breaks glass a number of tiny fragments may be transferred to that person. If the glass is broken in the commission of a crime then these fragments may be used as evidence. A Bayesian interpretation of this evidence relies on, among other things, the forensic scientist's ability to assess the likelihood t...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(97)00194-1
更新日期：1998-02-16 00:00:00
abstract：:Positive (certain, absolute) identification of human remains needs a scientific comparison between ante mortem and post-mortem biologic features, as fingerprint, odontological, radiological or DNA comparisons. X-rays comparison has been extensively used, usually comparing some peculiarities such as outlines of the bon...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.09.019
更新日期：2014-12-01 00:00:00
abstract：:In this study, we resolved the allelic distribution of three Y chromosomal short tandem repeats (STRs), DYS19 (n=218), DYS390 (n=257) and DYS393 (n=215), in a population study of 257 unrelated men living in the area of South Wüerttemberg/Hohenzollern. In DYS19 and in DYS393, five different alleles were discovered, and...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(00)00195-x
更新日期：2000-09-11 00:00:00
abstract：:The melting behavior of 28 high density polyethylene (HDPE) pipe samples manufactured and supplied by 13 different manufacturers in India was examined by 'differential scanning calorimetry (DSC)' to find out if this parameter could be used in differentiating between these HDPE pipe samples which are chemically the sam...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2007.05.020
更新日期：2008-03-05 00:00:00
abstract：:The effects of absorption time, titer of antiserum or lectin in the absorption stage, and elution temperature on relative antibody or lectin yield in elutes in the absorption-elution procedure were studied in bloodstains and ammoniacal extracts of bloodstains using conventional grouping reagents. In addition, monoclon...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(86)90182-9
更新日期：1986-07-14 00:00:00
