Abstract:
:Clinical and research-based tests in molecular biology require a substantial amount of DNA and RNA, unfortunately, a considerable number of cells is needed for this amount of sample. Blood is one of the best and easiest source of cells, but is not used due to its invasive drawing methods and the needed volume. Another considerable point is the low amount of samples detected in crime scenes. TRI reagent is one of the most available methods for DNA, RNA and protein extraction. However, based on unsuccessful results, this method has not been widely used on blood samples. In this study, for the first time, the use of TRI reagent on micro scale blood volume was reported, resulting in high yield of DNA and RNA with great quality.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Farivar S,Ghazimoradi MHdoi
10.1016/j.forsciint.2019.109931subject
Has Abstractpub_date
2019-10-01 00:00:00pages
109931eissn
0379-0738issn
1872-6283pii
S0379-0738(19)30343-3journal_volume
303pub_type
杂志文章abstract::Oral fluid (collected with the Intercept((R)) device) and plasma samples were obtained from 139 individuals suspected of driving under the influence of drugs and analyzed for Delta(9)-tetrahydrocannabinol (THC), the major psychoactive constituent of cannabis, using a validated quantitative LC-MS-MS method. The first a...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.03.033
更新日期:2006-09-12 00:00:00
abstract::The present study was made on an experimental animal model of a death from anaphylaxis, in which postmortem changes in levels of histamine and 1-methylhistamine, in whole blood were measured. Instead of the usual immunological method administering compound 48/80, a degranulating agent of mast cell and the effect close...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(90)90233-o
更新日期:1990-03-01 00:00:00
abstract::One hundred and ten pairs of blood and semen samples and their stains were studied to type glyoxalase 1 (GLO 1) isoenzymes using agarose-starch medium. A good agreement was observed between the phenotypes expressed in blood and semen samples of the same donor. No GLO 1 activity however could be demonstrated in the vag...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(88)90185-5
更新日期:1988-05-01 00:00:00
abstract::A very serious case of DFSA (drug facilitated sexual assault) is presented, in which a six-year-old girl died following sedation with γ-hydroxybutyric acid (GHB). She had been sexually abused by a relative. Samples of cardiac blood, bile, vitreous humour, liver, kidney, brain tissues and hair were analysed by a LC-MS/...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.12.044
更新日期:2016-02-01 00:00:00
abstract:BACKGROUND:Internationally, urine on-site testing has been used for detecting drivers under the influence of drugs (DUID) but more and more countries, such as Belgium, are switching to oral fluid screening. OBJECTIVE:To compare the previous (published in 1999) and current (published 2009) enforcement procedures of DUI...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.01.034
更新日期:2015-04-01 00:00:00
abstract::This chapter employs the fracture assessment triad, a systematic analytical approach, as a vehicle to explore the components used to interpret gunshot trauma to the cranium. First, a list of pertinent observations associated with gunshot trauma to the cranium is presented with a brief description of each. These featur...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.05.019
更新日期:2019-08-01 00:00:00
abstract::Torture appears to be a permanent feature in countries, which have experienced military coups or ruled by oppressive governments in the past, such as Turkey. The Human Rights Foundation of Turkey (HRFT) was established in 1990 to serve torture victims, mainly those who were the victims of the 1980 military regime. Sin...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.12.019
更新日期:2013-03-10 00:00:00
abstract::A total of 13 STR loci were typed from over 200 Caucasians from the Strathclyde region in the west of Scotland. ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00317-0
更新日期:2001-02-15 00:00:00
abstract::Chemicals associated with clandestine drug laboratories are often disposed of covertly into soil, sewerage systems, or public waste management facilities. There are two significant issues relating to such dumps of materials; they might contain valuable evidence as to drug manufacture, and they might be a source of pol...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(03)00110-5
更新日期:2003-06-24 00:00:00
abstract::In this report we describe a first suicide case in Serbia related to ingestion of Taxus baccata leaves. A 30-year old woman was found dead, and the green plant material in a plastic bag was found near her bed. Autopsy revealed dark green needle-like leaves in the stomach, similar to that contained in the plastic bag, ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.07.017
更新日期:2018-09-01 00:00:00
abstract::There is considerable value in developing tools capable of accurately and reliably determining when bruises were inflicted in humans. Previous work has focused on the visual changes observed in a bruise as the injury develops and heals. However, due to variables such as how and where on the body the bruise was inflict...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.04.036
更新日期:2013-09-10 00:00:00
abstract::Synthetic cannabinoids are a group of new psychoactive compounds (NPS) that act as agonists at the cannabinoid receptor. First reported in 2008, they currently represent one of the largest groups of NPS that are monitored by the European Monitoring Centre for Drugs and Drug Addiction (EMCDDA). Five samples (4 from the...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.10.020
更新日期:2017-12-01 00:00:00
abstract::Human teeth are biological structures that resist extreme conditions thus becoming a useful source of DNA for human forensic identification purposes. When it is possible, forensic prefer only non-damaged teeth whereas those with cavities are usually rejected to avoid both external and internal bacterial contamination....
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.08.021
更新日期:2015-12-01 00:00:00
abstract::Bullet embolism is a well known but a relatively rare phenomenon in common medicolegal autopsy practice. We present a homicide case of a bullet embolism to the left popliteal artery with interesting trajectory of projectile through the chest wall and right heart ventricle into the aortic lumen. Since in our presented ...
journal_title:Forensic science international
pub_type: 杂志文章,评审
doi:10.1016/j.forsciint.2003.09.014
更新日期:2004-01-06 00:00:00
abstract::Suicide attempts can be described to include all actions taken by an individual to end their life as a result of acute desperation. In parallel with the changes in the make-up of human societies both globally and in this country in recent years, suicide attempts are receiving attention in ever increasing proportions. ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00237-1
更新日期:2000-09-11 00:00:00
abstract::Synthetic hair-growth compounds have been illegally used in diverse products to enhance the short-term efficacy of these products. In this study, a rapid and simultaneous method for the determination of hair-growth compounds in adulterated products based on ultra high pressure liquid chromatography-tandem mass spectro...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.12.042
更新日期:2018-03-01 00:00:00
abstract::Age estimation is a vital aspect in creating a biological profile and aids investigators by narrowing down potentially matching identities from the available pool. In addition to routine casework, in the present global political scenario, age estimation in living individuals is required in cases of refugees, asylum se...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.04.004
更新日期:2013-09-10 00:00:00
abstract::Site and temporal changes in tranylcypromine (TCP) and lithium concentrations in blood were studied in a human poisoning case. Blood samples from peripheral vessels and six central vessels were obtained at 0, 6, 24, 48 and 72 h after starting the autopsy. Nine tissue samples were obtained on completion. TCP showed pre...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(93)90157-6
更新日期:1993-05-01 00:00:00
abstract::ABO groups were investigated on skin (and muscle), bone and hair specimens from 14 Egyptian mummies dating from the Roman period. Samples were tested by the AE (absorption-elution), MA (mixed agglutination) and HIF (histo-immunofluorescence) methods, in order to evaluate the reliability of each method. For half of the...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(89)90130-8
更新日期:1989-10-01 00:00:00
abstract::The validity of the determination of particle size distribution for forensic soil identification was studied using 73 soil samples collected from a 20 km x 15 km area. Soil particles were first divided into two fractions by wet sieving with a sieve with a 0.05 mm aperture size. Dry sieving was used to analyze the coar...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(01)00490-x
更新日期:2001-11-01 00:00:00
abstract::An improved technique for cocaine extraction from urine samples for gas chromatographic (GC) analysis is described. Employing a simple liquid-liquid extraction (LLE) of cocaine with a mixture of ethyl ether:isopropanol (9:1) the method presents a mean recovery of 74.49%. Limit of detection (LOD) and limit of quantific...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00124-x
更新日期:2002-07-17 00:00:00
abstract::An interrelation between consumption of illegal drugs and committing an indictable offence has been repeatedly discussed in literature. In a retrospective study serum concentrations of illegal and legal drugs as well as data originating from police reports and examinations by physicians taking blood from individuals b...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.01.031
更新日期:2013-03-10 00:00:00
abstract::Amphetamine and related derivatives are widely abused central- and psychostimulants. Detection of certain derivatives, such as methcathinone, by commonly available immunoassay screening techniques is insufficient. Multi-analyte confirmations for amphetamine type stimulants are therefore required, but traditional gas c...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2008.10.021
更新日期:2009-01-10 00:00:00
abstract::This is the first report of an ongoing study of insect succession on carrion carried out in Medellín, Colombia, using pigs (Sus scrofa) as a model to determine the insect sequence over 207 days. During this period, 2314 insects belonging to the following orders and families were collected: Diptera: Calliphoridae, Musc...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(01)00422-4
更新日期:2001-08-15 00:00:00
abstract::Due to inherent differences between terrestrial and aquatic systems, methods for estimating the postmortem interval (PMI) are not directly applicable to remains recovered from water. Recent studies have explored the use of microbial succession for estimating the postmortem submersion interval (PMSI); however, a non-di...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110480
更新日期:2021-01-01 00:00:00
abstract::Pyrethroids, organic compounds similar to natural pyrethrums, constitute the majority of insecticides. Pyrethroids are widely used around the world owing to their excellent selective toxicity to certain insects. In addition, they are easily found in daily life, accounting for most household pesticides. Owing to the ea...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.06.004
更新日期:2019-09-01 00:00:00
abstract::The article is devoted to the comparative analysis of effectiveness of the most widely used methods for medico-legal examination of the hyoid bone and laryngeal cartilages, i.e. palpation, radiography and complete preparation. A consecutive study of 137 cases of hanging (including 20 cases of roentgenological analysis...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(97)00101-1
更新日期:1997-08-22 00:00:00
abstract::The 3,4-methylenedioxy ring-substituted amphetamines, including "ADAM' and "EVE', are currently popular drugs of abuse. Adverse reactions are reported in the clinical literature but few fatal cases are documented and little toxicological data is available to guide those determining the cause or manner of death in such...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(95)01846-8
更新日期:1996-01-12 00:00:00
abstract::Superimposition is an efficient method for evaluation of coincidence between a skull and a photo portrait. The principle of superimposition method lies in the projection of the skull into the face image. During the projection of an object with a perspective camera, the mapping of a three-dimensional object into a two-...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.06.062
更新日期:2007-04-11 00:00:00
abstract::Data from the literature suggest that cases of sepsis complicated by right ventricular (RV) dysfunction have poorer prognosis. In these cases progressive hypoperfusion associated to increasing, injury-related, pulmonary vascular resistance account for RV ischemia. In the present analysis, we wanted to evaluate whether...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.12.002
更新日期:2016-02-01 00:00:00