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A comparative in situ decomposition study using still born piglets and leaf litter from a deciduous forest.

Abstract：

:A cadaver and dead plant organic matter, or litter, are rich energy sources that undergo a complex decomposition process, which impact the surrounding environmental microbiota. Advances in molecular microbiology techniques, with study of the 16S RNA genes, in particular, have highlighted the application of forensic ecogenomics in addressing key knowledge gaps. To investigate subsurface microbiome shifts as a novel tool to establish "postmortem microbial clock" and augment postmortem interval (PMI) and time-since-burial estimations, an in situ study with triplicate underground burials of piglets as human taphonomic proxies and Quercus robur leaf litter was monitored for 270 days. Changes in microbial community structure and composition were related directly to changes in seasonal temperature, with microbial shifts more pronounced during the summer. For example, Methylococcaceae could be used as seasonal bacterial indicators, from winter to summer, in establishing postmortem microbial clock for this site. Furthermore, Methylophilaceae (Methylophilales order) and Anaerolineaceae would differentiate for the piglet and leaf litter soils, respectively, 180 days after internment.

journal_name

Forensic Sci Int

journal_title

Forensic science international

authors

Olakanye AO,Nelson A,Ralebitso-Senior TK

doi

10.1016/j.forsciint.2017.04.024

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

85-92

eissn

0379-0738

issn

1872-6283

pii

S0379-0738(17)30170-6

journal_volume

276

pub_type

杂志文章

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