Abstract：
:This paper presents an overview of the organisation and the results of the collaborative exercises (CE) of the European DNA Profiling (EDNAP) Group's mitochondrial DNA population database project (EMPOP). The aim of the collaborative exercises was to determine whether uniformity of mtDNA sequencing results could be achieved among different laboratories. These were asked to sequence either the complete mtDNA control region or the two hypervariable regions HVI (16024-16365) and HVII (73-340) from DNA extracts, buccal swabs or bloodstains, proceeding in accordance with the protocol and strategies used in each individual laboratory. The results of the collaborative exercises were employed to identify possible sources of errors that could arise during the analysis and interpretation of mtDNA profiles. These findings were taken as a basis to tentatively make suitable arrangements for the construction of a high quality mtDNA database. One hundred fifty mtDNA profiles were submitted to the evaluating laboratory, and disaccording profiles were classified into four groups corresponding to the source of error: clerical errors, sample mix-ups, contaminations and discrepancies with respect to the mtDNA nomenclature. Overall, 14 disaccording haplotypes (16 individual errors) were observed. The errors included 10 clerical errors, 3 interpretation problems, 2 cases of sample mix-up and 1 case of point heteroplasmic mixture, where the 2 sequencing reactions brought inconsistent base calls. This corresponds to an error rate of 10.7% in a virtual mtDNA database consisting of the collaborative exercise results. However, this estimate is still conservative compared to conclusions drawn by authors of meanwhile numerous publications critically reviewing published mtDNA population databases. Our results and earlier published concerns strongly emphasize the need for appropriate safety regulations when mtDNA profiles are compiled for database purposes in order to accomplish the high standard required for mtDNA databases that are used in the forensic context.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Parson W,Brandstätter A,Alonso A,Brandt N,Brinkmann B,Carracedo A,Corach D,Froment O,Furac I,Grzybowski T,Hedberg K,Keyser-Tracqui C,Kupiec T,Lutz-Bonengel S,Mevag B,Ploski R,Schmitter H,Schneider P,Syndercombe-Courtdoi
10.1016/j.forsciint.2003.11.008keywords:
subject
Has Abstractpub_date
2004-01-28 00:00:00pages
215-26issue
2-3eissn
0379-0738issn
1872-6283pii
S0379073803004936journal_volume
139pub_type
杂志文章abstract：OBJECTIVE:To evaluate the clinical utility of glycolic acid (GA) determination in the diagnosis and prognosis of ethylene glycol (EG) intoxications. METHOD:Systematic review of serum and/or urine GA concentrations available in the literature in cases of EG poisoning. Present a clinical case in which the determination ...
journal_title：Forensic science international
pub_type： 杂志文章,评审
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journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：2005-05-10 00:00:00
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journal_title：Forensic science international
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journal_title：Forensic science international
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：2004-12-02 00:00:00
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journal_title：Forensic science international
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更新日期：1996-07-12 00:00:00
abstract：:In this follow-up study the collaboration between two research groups from the USA and the Netherlands was continued to expand the framework of chemical attribution for the homemade explosive erythritol tetranitrate (ETN). Isotope ratio mass spectrometry (IRMS) analysis was performed to predict possible links between ...
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更新日期：2020-08-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2004.06.011
更新日期：2005-04-20 00:00:00
