Abstract：
:The DNA identification of decomposed human remains for coronial investigations at the Victorian Institute of Forensic Medicine routinely requires the retrieval and processing of a bone sample obtained from the deceased. Bone is a difficult sample type to work with as it requires surgical removal from the deceased, refrigerated storage, and additional processing steps prior to DNA analysis in comparison to other samples types such as buccal swabs or blood stains. In an attempt to overcome the issues posed by bone, a DNA extraction method utilising toenails as an alternate source material was optimised and trialled. Two DNA extraction methods were optimised for digestion of toenail material, with the method utilising the QIAGEN DNA Investigator Kit selected for a casework trial. Single source DNA profiles, matching those of the conventional samples taken, were obtained for toenail samples collected from 28 of 30 coronial cases available for this study. Of these, 26 toenail samples produced full profiles. Although the overall DNA profile quality from the toenails was less than that of the conventional sample, the profiles from toenails met the reporting requirements for identification. Based on the results obtained, the Victorian Institute of Forensic Medicine will be implementing toenails as the primary sample type for collection from decomposed remains when blood is not a suitable sample type.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Schlenker A,Grimble K,Azim A,Owen R,Hartman Ddoi
10.1016/j.forsciint.2015.10.025subject
Has Abstractpub_date
2016-01-01 00:00:00pages
1-10eissn
0379-0738issn
1872-6283pii
S0379-0738(15)00451-Xjournal_volume
258pub_type
杂志文章abstract：:Restriction fragment length polymorphism analysis (RFLP-analysis) was carried out on blood samples from 616 unrelated Danish Caucasians. DNA was restricted with Hinf I and analyzed with the single locus variable number tandem repeats (VNTR) probes MS1, MS31, MS43a and YNH24. The effect of the sample size on the estima...
journal_title：Forensic science international
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journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：1994-06-10 00:00:00
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journal_title：Forensic science international
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journal_title：Forensic science international
pub_type： 杂志文章
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journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：2006-03-10 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2010.02.018
更新日期：2010-06-15 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：2013-04-10 00:00:00
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pub_type： 杂志文章
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更新日期：1988-12-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(94)01612-9
更新日期：1995-01-05 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.09.005
更新日期：2013-12-10 00:00:00
abstract：:This study investigated the effect of seasonal variables on decomposition in the early post-mortem period using 26 donated human cadavers at the University of Tennessee's Anthropology Research Facility (ARF), USA. The rate and pattern of decomposition in human cadavers (as measured by TBS and the revised TBSsurf metho...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2020.110419
更新日期：2020-10-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2012.05.025
更新日期：2012-10-10 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(90)90252-t
更新日期：1990-02-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.08.007
更新日期：2006-06-02 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2007.03.027
更新日期：2007-08-06 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.03.048
更新日期：2019-07-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2008.12.024
更新日期：2009-04-15 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：2008-01-15 00:00:00
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pub_type： 杂志文章
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journal_title：Forensic science international
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更新日期：2005-10-29 00:00:00
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pub_type： 杂志文章
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更新日期：1983-05-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：2016-09-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：2001-12-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章,评审
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更新日期：1998-04-05 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(95)01846-8
更新日期：1996-01-12 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(01)00428-5
更新日期：2001-08-15 00:00:00
abstract：:Urine is often the sample of choice for drug screening in aviation/general forensic toxicology and in workplace drug testing. In some instances, the origin of the submitted samples may be challenged because of the medicolegal and socioeconomic consequences of a positive drug test. Methods for individualization of biol...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(99)00034-1
更新日期：1999-05-31 00:00:00
