Isolation of the DNA minisatellite probe MZ 1.3 and its application to DNA 'fingerprinting' analysis.

Abstract:

:A minisatellite probe, MZ 1.3, detecting hypervariable fragment patterns was isolated from a human genomic library. A repetitive sequence of 27 bp length was identified which is contained in the probe approx. 40 times. The MZ 1.3 repeat shows variable homology of 53-73% to the repetitive sequence of the protein III gene of the bacteriophage M13 genome. Polymorphic restriction fragment patterns were found with MZ 1.3 using the enzymes Hinf I, BstN I, Hae III, Mbo I, PstI/Pvu II, and Rsa I. An average of 18 polymorphic fragments was observed using Hinf I as enzyme. The band sharing frequency after Hinf I digestion among unrelated individuals was determined to be 23.8 +/- 7.2%. An example for the application of MZ 1.3 to paternity testing in an incest case is given. The probe can be used with radioactive or non-radioactive detection systems. An approach is presented to compare polymorphic fragment patterns from individuals obtained by independent gel runs on the basis of relative band positions (RBP) and calculated in a computerized analysis.

journal_name

Forensic Sci Int

authors

Schacker U,Schneider PM,Holtkamp B,Bohnke E,Fimmers R,Sonneborn HH,Rittner C

doi

10.1016/0379-0738(90)90252-t

subject

Has Abstract

pub_date

1990-02-01 00:00:00

pages

209-24

issue

2-3

eissn

0379-0738

issn

1872-6283

pii

0379-0738(90)90252-T

journal_volume

44

pub_type

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