Abstract:
:A minisatellite probe, MZ 1.3, detecting hypervariable fragment patterns was isolated from a human genomic library. A repetitive sequence of 27 bp length was identified which is contained in the probe approx. 40 times. The MZ 1.3 repeat shows variable homology of 53-73% to the repetitive sequence of the protein III gene of the bacteriophage M13 genome. Polymorphic restriction fragment patterns were found with MZ 1.3 using the enzymes Hinf I, BstN I, Hae III, Mbo I, PstI/Pvu II, and Rsa I. An average of 18 polymorphic fragments was observed using Hinf I as enzyme. The band sharing frequency after Hinf I digestion among unrelated individuals was determined to be 23.8 +/- 7.2%. An example for the application of MZ 1.3 to paternity testing in an incest case is given. The probe can be used with radioactive or non-radioactive detection systems. An approach is presented to compare polymorphic fragment patterns from individuals obtained by independent gel runs on the basis of relative band positions (RBP) and calculated in a computerized analysis.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Schacker U,Schneider PM,Holtkamp B,Bohnke E,Fimmers R,Sonneborn HH,Rittner Cdoi
10.1016/0379-0738(90)90252-tsubject
Has Abstractpub_date
1990-02-01 00:00:00pages
209-24issue
2-3eissn
0379-0738issn
1872-6283pii
0379-0738(90)90252-Tjournal_volume
44pub_type
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