Abstract:
:A collaborative study on Raman spectroscopy and microspectrophotometry (MSP) was carried out by members of the ENFSI (European Network of Forensic Science Institutes) European Fibres Group (EFG) on different dyed cotton fabrics. The detection limits of the two methods were tested on two cotton sets with a dye concentration ranging from 0.5 to 0.005% (w/w). This survey shows that it is possible to detect the presence of dye in fibres with concentrations below that detectable by the traditional methods of light microscopy and microspectrophotometry (MSP). The MSP detection limit for the dyes used in this study was found to be a concentration of 0.5% (w/w). At this concentration, the fibres appear colourless with light microscopy. Raman spectroscopy clearly shows a higher potential to detect concentrations of dyes as low as 0.05% for the yellow dye RY145 and 0.005% for the blue dye RB221. This detection limit was found to depend both on the chemical composition of the dye itself and on the analytical conditions, particularly the laser wavelength. Furthermore, analysis of binary mixtures of dyes showed that while the minor dye was detected at 1.5% (w/w) (30% of the total dye concentration) using microspectrophotometry, it was detected at a level as low as 0.05% (w/w) (10% of the total dye concentration) using Raman spectroscopy. This work also highlights the importance of a flexible Raman instrument equipped with several lasers at different wavelengths for the analysis of dyed fibres. The operator and the set up of the analytical conditions are also of prime importance in order to obtain high quality spectra. Changing the laser wavelength is important to detect different dyes in a mixture.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Massonnet G,Buzzini P,Monard F,Jochem G,Fido L,Bell S,Stauber M,Coyle T,Roux C,Hemmings J,Leijenhorst H,Van Zanten Z,Wiggins K,Smith C,Chabli S,Sauneuf T,Rosengarten A,Meile C,Ketterer S,Blumer Adoi
10.1016/j.forsciint.2012.05.025subject
Has Abstractpub_date
2012-10-10 00:00:00pages
200-7issue
1-3eissn
0379-0738issn
1872-6283pii
S0379-0738(12)00273-3journal_volume
222pub_type
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