Abstract:
:A method for identifying human skeletal muscle by detection of human myoglobin using a double-sandwich ELISA was developed. When an extract was prepared from 0.1 g skeletal muscle homogenized with 10 ml PBS, this method was able to detect human myoglobin in extracts diluted 10(4)-fold. There was no difference in the detection limit between individuals or sites of origin of skeletal muscles. Species specificity was good and no cross reaction occurred with skeletal muscle from other animals except the gorilla. Our method could also discriminate between skeletal muscle and other organs or tissues except the heart. Human myoglobin could be detected in skeletal muscles under the following conditions: putrefied at room temperature for 5 months, dried at room temperature for 11 months, heated at 100 degrees C for 72 h and immersed in fresh water at room temperature for 6 days. Two practical cases to which this method was applied are presented.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Kitao T,Miyaishi S,Ishizu Hdoi
10.1016/0379-0738(94)01667-4subject
Has Abstractpub_date
1995-02-28 00:00:00pages
205-14issue
3eissn
0379-0738issn
1872-6283pii
0379073894016674journal_volume
71pub_type
杂志文章abstract::A purge and trap concentrator with a Tenax trap was coupled to gas chromatography-Fourier transform infrared spectrometry for the identification of volatile organic compounds in blood samples. A styrene-divinyl benzene porous layer capillary column allowed the separation of compounds such as household and medical gase...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(96)01912-3
更新日期:1996-07-12 00:00:00
abstract::The identification of forensically important blowflies of the genus Chrysomya (Diptera: Calliphoridae) may be hampered by their close morphological similarities, especially as immatures. In contrast to most previous studies, the utility of a nuclear rather than mitochondrial genetic marker was investigated to solve th...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2008.01.009
更新日期:2008-05-20 00:00:00
abstract::Sudden unexplained nocturnal death syndrome (SUNDS) is a conundrum to both forensic pathologists and physicians, more than 80% of which the molecular pathogenesis remains unclear. Reported studies on both clinical and genetic phenotypes suggest SUNDS is related to congenital and acquired arrhythmias. Recent researches...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2016.12.006
更新日期:2017-01-01 00:00:00
abstract::Stabbings have been reported as the most frequent violent crime in countries where there is a severe restriction on obtaining firearms, such as the United Kingdom. Knives, scissors, and screwdrivers, as studied in this research, are among the most commonly encountered weapons involving stabbing events. When any of the...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.05.027
更新日期:2019-08-01 00:00:00
abstract::Allele frequencies for seven STRs loci were obtained from a sample of 215 unrelated healthy Italian individuals. ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(01)00513-8
更新日期:2001-11-15 00:00:00
abstract::Missing, ineligible or delayed reference data to establish conventional dental or DNA identification are common scenarios in forensic practice. Therefore, it is worthwhile to explore new avenues that facilitate human identification. Due to the recent remarkable evolution in the prosthetic dental restorations based on ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.109926
更新日期:2019-09-01 00:00:00
abstract::Despite the fact that the differences in epidermal ridge density between men and women have been accepted for some time, they have only been thoroughly demonstrated in a small number of populations. The aim of this study is to determine whether such differences exist in a sample of the Spanish population by counting e...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2008.06.014
更新日期:2008-08-25 00:00:00
abstract::The technique of isoelectric focusing (IEF) in ultra-thin polyacrylamide gels as a method of phenotyping erythrocyte acid phosphatase (EAP) has been applied to a large number of red cell lysate and dried bloodstains. This paper presents the results of this study and discusses some features of the IEF patterns and prob...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(81)90141-9
更新日期:1981-07-01 00:00:00
abstract::The aim of the study was to improve the objectivity of X-ray image comparison for the identification of unknown dead individuals. CT-data were collected for 30 macerated skulls. An already presented computer program which uses CT data to establish virtual X-ray images was used to obtain X-rays with different beam angu...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00452-7
更新日期:2001-03-01 00:00:00
abstract::Allele frequency data and forensic efficiency parameters for 10 STR loci: D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01 and FGA, were estimated from a sample of 207 unrelated individuals in Lodz region of Poland. ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(03)00268-8
更新日期:2003-10-14 00:00:00
abstract::Despite indicating numerous demographic features such as sex, stature, and age; the sacrum remains a relatively insufficiently researched skeletal element. A set pattern of ossification and fusion of the sacrum makes it a useful bone for estimating age-at-death in unknown skeletal individuals. The aims of this study, ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.02.011
更新日期:2018-05-01 00:00:00
abstract::The experience of the Northern Ireland State Pathology Service is drawn on to illustrate the forensic aspects of death by shooting and bombing caused by terrorists. The investigations into these deaths have to contend with rumours and allegations which can inflame feelings in the community or mislead the investigators...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(88)90215-0
更新日期:1988-01-01 00:00:00
abstract::It is a requirement that forensic DNA profiling evidence be accompanied by an estimation of its weight, in order that the court can assign an appropriate probative value to it during legal proceedings. There are various models by which this estimation can be made, but each relies on approximations of the allele freque...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2007.04.221
更新日期:2008-03-05 00:00:00
abstract::V. M. E. was evacuated when he was a young boy in 1939. He left an aunt and cousins in Spain (G. E. family). He was adopted in Belgium by the D. family and thus his new name became V. D. He has been unable to remember his childhood before his adoption, a symptomatology compatible with amnesia for personal identity, pr...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.03.014
更新日期:2019-05-01 00:00:00
abstract::This paper focuses on likelihood ratio based evaluations of fibre evidence in cases in which there is uncertainty about whether or not the reference item available for analysis - that is, an item typically taken from the suspect or seized at his home - is the item actually worn at the time of the offence. A likelihood...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.02.021
更新日期:2012-07-10 00:00:00
abstract::During a stabbing, apparel fabrics are usually damaged and may be penetrated. Despite numerous studies considering forces required to penetrate skin and human stabbing performance, none have systematically evaluated which variables affect severance appearance from a textile science perspective using a human stabbing p...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.01.024
更新日期:2015-04-01 00:00:00
abstract::In this research, the allelic distribution of 15 tetrameric short tandem repeats (STR) loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA) were obtained from 167 Mulao and 108 Maonan unrelated individuals. The Genepop v3.4 and the PowerStats v1.2 we...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.06.023
更新日期:2006-10-16 00:00:00
abstract::To investigate the pathological changes in the heart induced by pulmonary embolism, 20 autopsy cases of pulmonary embolism and 10 control cases of acute death from traumatic injury were examined. Adding to the routine hematoxylin-eosin (HE) staining, immunostaining with CD68 pan-macrophage marker was performed on the ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(03)00138-5
更新日期:2003-07-08 00:00:00
abstract::Capillary electrophoresis, the modern approach to instrumental electrophoresis, is probably the most rapidly expanding analytical technique that has appeared in recent years. In the hands of forensic toxicologists, capillary electrophoresis (CE) represents a powerful new analytical tool, which has proved suitable for ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(94)01612-9
更新日期:1995-01-05 00:00:00
abstract::Understanding bone trauma characteristics is a fundamental component of forensic investigations that can assist in understanding the nature of blunt trauma related deaths. The variation of each blunt force trauma (BFT) injury is dependent on a magnitude of factors including, age, sex, health, angle of impact, impact m...
journal_title:Forensic science international
pub_type: 杂志文章,评审
doi:10.1016/j.forsciint.2020.110140
更新日期:2020-02-01 00:00:00
abstract::Forensic personnel may face a daunting task when searching for buried weapons at crime scenes or potential disposal sites. In particular, it is common to search for a small firearm that was discarded or buried by a perpetrator. When performing forensic searches, it is recommended to first use non-invasive methods such...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2010.10.019
更新日期:2011-05-20 00:00:00
abstract:INTRODUCTION:Postmortem redistribution (PMR), a well-described phenomenon in forensic toxicology for certain drugs, can result in increased central blood concentrations relative to peripheral blood concentrations. Δ(9)-tetrahydrocannabinol (THC), the primary psychoactive component in cannabis or marijuana, is the illic...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2011.06.028
更新日期:2011-10-10 00:00:00
abstract::This study focused on the frequency of pathological acid gastroesophageal reflux (AGER) on 50 children considered to be at risk for sudden infant death syndrome: that is to say, 30 near misses and 20 subsequent siblings. Four parameters were studied in a 24-h pH metry: (1) The percentage of time spent at pH less than ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(88)90152-1
更新日期:1988-02-01 00:00:00
abstract::In this study, we resolved the allelic distribution of three Y chromosomal short tandem repeats (STRs), DYS19 (n=218), DYS390 (n=257) and DYS393 (n=215), in a population study of 257 unrelated men living in the area of South Wüerttemberg/Hohenzollern. In DYS19 and in DYS393, five different alleles were discovered, and...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00195-x
更新日期:2000-09-11 00:00:00
abstract::The abuse or misuse of forged erectile-dysfunction drugs, containing phosphodiesterase type 5 inhibitors (e.g. sildenafil), is a serious issue globally. Therefore, the detection of sildenafil and related active analogues in counterfeit pharmaceuticals or the differentiation between counterfeit and authentic drugs has ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.08.016
更新日期:2015-12-01 00:00:00
abstract::The typing of the D1S8 (MS32) locus using the minisatellite variant repeat (MVR) polymerase chain reaction (PCR) method was performed by visualising amplified DNA stained with ethidium bromide. The results from rapid detection MVR-PCR were compared with those from the original MVR-PCR using Southern blot hybridisation...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(94)90321-2
更新日期:1994-05-25 00:00:00
abstract::5F-MDMB-PICA has been detected in products sold on the internet as well as in biological samples since 2016. It is associated with serious adverse health and behavioral effects and even death. Herein we report on twelve cases with proven 5F-MDMB-PICA consumption, including three fatalities, four cases of driving under...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110410
更新日期:2020-09-01 00:00:00
abstract::Seven Y-specific STR loci (DYS19, DYS389I, DY5389II, DYS390, DYS391, DYS392 and DYS393) were studied in five populations from the Iberian Peninsula: Andalusia, Valencia, Basque Country, Galicia and Northern Portugal. Haplotype and allele frequencies of these seven Y-chromosome STRs were estimated. Observed haplotype d...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00156-0
更新日期:2000-05-15 00:00:00
abstract::This paper presents real world acceleration data for an ambulance driving up and over a curb. A full scale reenactment was performed for a litigated case in which a patient on a gurney in an ambulance claimed a variety of bodily injuries after the ambulance struck a curb. A height and weight matched surrogate rode on ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.01.023
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND:Multiple genome-wide association studies (GWAS) and targeted gene sequencing have identified common variants in SCN10A in cases of PR and QRS duration abnormalities, atrial fibrillation and Brugada syndrome. The New York City Office of Chief Medical Examiner has now also identified five SCN10A variants of un...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.05.042
更新日期:2019-08-01 00:00:00