DNA typing of the D1S8 (MS32) locus by rapid detection minisatellite variant repeat (MVR) mapping using polymerase chain reaction (PCR) assay.

Abstract:

:The typing of the D1S8 (MS32) locus using the minisatellite variant repeat (MVR) polymerase chain reaction (PCR) method was performed by visualising amplified DNA stained with ethidium bromide. The results from rapid detection MVR-PCR were compared with those from the original MVR-PCR using Southern blot hybridisation with a 32P-labelled probe. With genomic DNA extracted from blood samples of 40 healthy unrelated Japanese individuals, the first 41 codes, on average, were correctly determined by rapid detection MVR-PCR without band intensity information, compared with at least 60 codes typed by the original MVR-PCR. The rapid detection MVR-PCR method was applied to bloodstains to simulate forensic samples. On average, 39 code positions could be determined from DNA extracted from 3-month-old bloodstains of six persons. Rapid detection MVR-PCR is more convenient than the original MVR-PCR, furnishes much information with regard to personal identification, and should be applicable to forensic fields.

journal_name

Forensic Sci Int

authors

Yamamoto T,Tamaki K,Kojima T,Uchihi R,Katsumata Y,Jeffreys AJ

doi

10.1016/0379-0738(94)90321-2

subject

Has Abstract

pub_date

1994-05-25 00:00:00

pages

69-75

issue

1

eissn

0379-0738

issn

1872-6283

pii

0379-0738(94)90321-2

journal_volume

66

pub_type

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