Abstract：
:A novel 39-plex typing system for single nucleotide polymorphisms (SNPs) has been developed. This multiplex approach has the advantage of being able to type 38 autosomal SNPs and one sex-discriminating base exchange site on the X and Y chromosomes rapidly and simultaneously. The SNP loci on the autosomes, which we examined, contain 15 loci distributed on blood type genes: three on RhCE, two each on Km and Gc, and one each on Duffy, AcP1, Tf, MN, GPT, EsD, PI, and Kidd genes. Thirty-seven genomic DNA fragments containing a total of 38 SNPs and one sex-discriminating site were amplified in one multiplex PCR reaction. Following the reaction, single nucleotide primer extension reaction was performed by dividing these SNP loci into five groups. The SNP type of each of the 39 loci was determined at one time by capillary electrophoresis using the newly designed multi-injection method. The combined PD (power of discrimination) of this typing system was (1-1.1) x 10(-14), and the MEC (mean exclusion chance) was 0.9990. We applied this system to forensic cases, including 16 paternity testing cases (13 non-exclusion and three exclusion cases) and one personal identification case. For the paternity testing cases, the highest Essen-Möller's W-value was 0.9999995. The pM (matching probability) of the personal identification case was 2.22 x 10(-17). These data showed that this system was an excellent tool for use in forensic cases of paternity testing and personal identification.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Inagaki S,Yamamoto Y,Doi Y,Takata T,Ishikawa T,Imabayashi K,Yoshitome K,Miyaishi S,Ishizu Hdoi
10.1016/j.forsciint.2004.03.005keywords:
subject
Has Abstractpub_date
2004-08-11 00:00:00pages
45-57issue
1eissn
0379-0738issn
1872-6283pii
S037907380400146Xjournal_volume
144pub_type
杂志文章abstract：:Technology provides new ways to access customers and suppliers while enhancing the security of off-line criminal activity. Since the first cryptomarket, Silk Road, in 2011, cryptomarkets have transformed the traditional drug sale by facilitating the creation of a global network of vendors and buyers. Due to the fragme...
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journal_title：Forensic science international
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doi：10.1016/j.forsciint.2006.06.044
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abstract：:The systematic exhumation of mass graves is becoming a frequent occurrence globally. Historically, it has been mostly anthropologists with their particular expertise in osteology that have been engaged by non-governmental organizations (NGO) to monitor mass grave exhumations and postmortem examinations, conducted by a...
journal_title：Forensic science international
pub_type： 指南,杂志文章
doi：10.1016/s0379-0738(03)00124-5
更新日期：2003-07-08 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2011.02.012
更新日期：2012-02-10 00:00:00
abstract：:3-Methylmethcathinone (3-MMC or metaphedrone) is a synthetic cathinone, recently introduced in the market of the new psychoactive substances (NPS), initially to replace mephedrone (4-methylmethcathinone or 4-MMC), and rapidly widespread among drug users. 3-Methylmethcathinone is legally controlled in many countries, b...
journal_title：Forensic science international
pub_type： 杂志文章,评审
doi：10.1016/j.forsciint.2018.11.024
更新日期：2019-02-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2020.110473
更新日期：2020-11-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2008.10.018
更新日期：2009-01-10 00:00:00
abstract：:Anti-doping authorities have high expectations of the athlete steroidal passport (ASP) for anabolic-androgenic steroids misuse detection. However, it is still limited to the monitoring of known well-established compounds and might greatly benefit from the discovery of new relevant biomarkers candidates. In this contex...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2011.07.023
更新日期：2011-12-10 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(03)00259-7
更新日期：2003-09-09 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.07.025
更新日期：2018-10-01 00:00:00
abstract：:The case of a 13-month-old boy with a diagnosis of unclear unconsciousness is reported on. As the physical examination did not lead to any explanation of his condition, the administration of drugs in the context of Munchausen syndrome by proxy was suspected. Complex forensic-toxicological analyses using HPLC/UV, LC/MS...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.04.021
更新日期：2009-08-10 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.03.005
更新日期：2019-06-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.08.019
更新日期：2018-10-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.10.020
更新日期：2017-12-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2011.12.003
更新日期：2012-06-10 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.07.009
更新日期：2016-10-01 00:00:00
abstract：:The aim of the present study was to determine the chronology of third molar mineralization and to establish Austrian reference data. Therefore, a cross-sectional study was undertaken by evaluating 610 panoramic radiographs in order to assess the mineralization status of the mandibular third molars of Austrian male and...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.08.014
更新日期：2007-07-04 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章,评审
doi：10.1016/j.forsciint.2006.05.009
更新日期：2007-01-17 00:00:00
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doi：10.1016/j.forsciint.2013.03.030
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journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：1992-11-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.07.023
更新日期：2014-09-01 00:00:00
abstract：AIM:To highlight some problems that may occur when investigating clozapine-associated deaths including (i) that death may be related to gastrointestinal hypomotility and (ii) that post-mortem blood clozapine and norclozapine concentrations may not reflect ante-mortem concentrations. CASE REPORTS:A 41-year-old male die...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2010.07.021
更新日期：2011-03-20 00:00:00
abstract：:This study explores the applicability of micro-Raman spectroscopy as a non-destructive technique for the analysis of color toner printed counterfeits. The main aim of the research paper was to find out whether Raman spectroscopy is a suitable method for establishing the connection between different specimens of counte...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2012.10.007
更新日期：2012-11-30 00:00:00
abstract：:This paper presents real world acceleration data for an ambulance driving up and over a curb. A full scale reenactment was performed for a litigated case in which a patient on a gurney in an ambulance claimed a variety of bodily injuries after the ambulance struck a curb. A height and weight matched surrogate rode on ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.01.023
更新日期：2014-04-01 00:00:00
abstract：:The chromium-doped zinc gallogermanate, Zn3Ga2Ge2O10:0.5%Cr3+, was prepared and modified for use as a near-infrared (NIR) luminescent fingerprint dusting powder. The optical properties of this material are illustrated and have been shown to offer versatility and promise in terms of overcoming troublesome backgrounds d...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.04.012
更新日期：2017-07-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(01)00596-5
更新日期：2001-12-27 00:00:00
abstract：:To investigate the pathological changes in the heart induced by pulmonary embolism, 20 autopsy cases of pulmonary embolism and 10 control cases of acute death from traumatic injury were examined. Adding to the routine hematoxylin-eosin (HE) staining, immunostaining with CD68 pan-macrophage marker was performed on the ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(03)00138-5
更新日期：2003-07-08 00:00:00
abstract：:Capillary high performance liquid chromatography (HPLC) was combined with frit fast atom bombardment (FAB) mass spectrometry (MS) and a detailed procedure has been established for on-line analysis of cefaclor in human serum. The capillary column (0.3 mm i.d.) enabled the introduction of entire effluent to the frit int...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(93)90313-y
更新日期：1993-04-01 00:00:00
