Semi-quantitative analysis of cefaclor in human serum by capillary high performance liquid chromatography/fast atom bombardment mass spectrometry.

Abstract:

:Capillary high performance liquid chromatography (HPLC) was combined with frit fast atom bombardment (FAB) mass spectrometry (MS) and a detailed procedure has been established for on-line analysis of cefaclor in human serum. The capillary column (0.3 mm i.d.) enabled the introduction of entire effluent to the frit interface for FAB-MS; and a special column switching device for injection and concentration enabled the injection of as large as a 500-microliters volume sample. These conditions gave much higher sensitivity than that of previous HPLC/MS system. Thus, low levels of cefaclor could be successfully identified in sera by its mass spectral measurements 2 h after its single oral administration of a 250-mg capsule in two subjects.

journal_name

Forensic Sci Int

authors

Sato K,Kobayashi K,Moore CM,Mizuno Y,Katsumata Y

doi

10.1016/0379-0738(93)90313-y

subject

Has Abstract

pub_date

1993-04-01 00:00:00

pages

71-7

issue

1

eissn

0379-0738

issn

1872-6283

pii

0379-0738(93)90313-Y

journal_volume

59

pub_type

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