Investigation of sterols as potential biomarkers for the detection of pig (S. s. domesticus) decomposition fluid in soils.

Abstract:

:This study was carried out to evaluate the potential of using cholesterol and coprostanol, as indicators for the detection of decomposition fluid of buried pigs (S. s. domesticus) in soils. In May 2007, four pig carcasses (∼35kg) were buried in shallow graves (∼40 cm depth) at the University of Ontario Institute of Technology in Canada. Two pigs were exhumed after three months (Pig 1, Pig 2) and six months (Pig 3, Pig 4) post burial. Soil samples were collected beneath the pig carcasses (∼40cm depth) and from grave walls (∼15-20 cm depth) as well as from a parallel control site. Coprostanol and cholesterol were extracted from soils, purified with solid phase extraction (SPE) and analysed with gas chromatography/mass spectrometry (GC/MS). A significant increase in cholesterol concentrations (p<0.05) and amounts of coprostanol were detected in soil located beneath the pig carcasses after three months of burial. It is assumed that during the putrefaction and liquefaction stages of decomposition pig fluid which contains cholesterol and coprostanol is released into the underlying soil. Therefore, cholesterol and coprostanol could be used as potential biomarkers to detect the presence of decomposition fluid three months after burial under comparable soil and environmental conditions. Further research is suggested for additional soil sampling before and after three months to investigate the abundance of these and other sterols.

journal_name

Forensic Sci Int

authors

von der Lühe B,Dawson LA,Mayes RW,Forbes SL,Fiedler S

doi

10.1016/j.forsciint.2013.03.030

subject

Has Abstract

pub_date

2013-07-10 00:00:00

pages

68-73

issue

1-3

eissn

0379-0738

issn

1872-6283

pii

S0379-0738(13)00186-2

journal_volume

230

pub_type

杂志文章
  • Morphine levels in urine subsequent to poppy seed consumption.

    abstract::Urine morphine levels after the consumption of poppy seeds were measured in two separate trials. Maximum levels of approximately 18 micrograms/ml were found using RIA, EMIT-ST and GC methodologies. Positive immunoassay results were seen up to 60 h post-ingestion. Several different lots of seeds from various sources we...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(85)90173-2

    authors: Fritschi G,Prescott WR Jr

    更新日期:1985-02-01 00:00:00

  • Experimental fragmentation of pipe bombs with varying case thickness.

    abstract::Among all the improvised explosive devices (IEDs) known, pipe bombs are one of the most popular devices used by terrorists. They are simple to use, easy to construct and materials are readily available. For this IED, fragmentation is the primary injury mechanism, which makes them a desirable weapon for terrorists aimi...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2019.110034

    authors: da Silva LA,Johnson S,Critchley R,Clements J,Norris K,Stennett C

    更新日期:2020-01-01 00:00:00

  • A comparative pharmacokinetic study of ethanol in the blood, vitreous humour and aqueous humour of rabbits.

    abstract::The pharmacokinetics of ethanol in the blood, vitreous humour and aqueous humour of the rabbit after intravenous administration of single dose have been compared. Ethanol levels were determined by gas-liquid chromatography. Certain parallelism between ethanol levels in these body fluids were observed after 80 min. ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(89)90236-3

    authors: Fernández P,López-Rivadulla M,Liñares JM,Tato F,Bermejo AM

    更新日期:1989-04-01 00:00:00

  • Method validation of a survey of thevetia cardiac glycosides in serum samples.

    abstract::A sensitive and specific liquid chromatography tandem mass spectrometry (HPLC-ESI(+)-MS/MS) procedure was developed and validated for the identification and quantification of thevetin B and further cardiac glycosides in human serum. The seeds of Yellow Oleander (Thevetia peruviana) contain cardiac glycosides that can ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2011.02.013

    authors: Kohls S,Scholz-Böttcher B,Rullkötter J,Teske J

    更新日期:2012-02-10 00:00:00

  • Gas chromatographic examination of postmortem specimens after maprotiline intoxication.

    abstract::Ingestion of an unknown quantity of Ludiomil (maprotiline)-costed tablets in a suicide is described. Although maprotiline is known for over 20 years now, relatively few cases of intoxications due to maprotiline overdose have been reported. The authors report a new and quick method to analyze and determine maprotiline ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(97)00064-9

    authors: Keller T,Zollinger U

    更新日期:1997-08-04 00:00:00

  • Forensic identification in teeth with caries.

    abstract::Human teeth are biological structures that resist extreme conditions thus becoming a useful source of DNA for human forensic identification purposes. When it is possible, forensic prefer only non-damaged teeth whereas those with cavities are usually rejected to avoid both external and internal bacterial contamination....

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2015.08.021

    authors: Alia-García E,Parra-Pecharromán D,Sánchez-Díaz A,Mendez S,Royuela A,Gil-Alberdi L,López-Palafox J,Del Campo R

    更新日期:2015-12-01 00:00:00

  • Suicide of a body packer.

    abstract::The smuggling of illicit drugs by means of body packing has become a common problem at European airports. Europe is considered to be the fastest growing market for cocaine worldwide, and the air route is the most frequently used method of trafficking cocaine. Smuggling illicit drugs by use of body packing is considere...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(99)00142-5

    authors: Stichenwirth M,Stelwag-Carion C,Klupp N,Hönigschnabl S,Vycudilik W,Bauer G,Risser D

    更新日期:2000-01-24 00:00:00

  • A study to visualize and determine the sequencing of intersecting ink lines.

    abstract::Determining the sequencing of intersecting ink lines is one of the current problems for forensic document examiners. The way two inks will distribute and interact with each other and the paper at the crossing is a dynamic process that can be affected by many variables. Thus, the main purpose of this manuscript is to v...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2013.10.026

    authors: Ozbek N,Braz A,López-López M,García-Ruiz C

    更新日期:2014-01-01 00:00:00

  • Fatal doxepin intoxication--suicide or slow gradual intoxication?

    abstract::The differentiation of intoxication courses is one of the most difficult challenges for forensic pathologists and toxicologists. The case of a 52-year-old female inpatient of a psychiatric clinic with multiple medications who died from doxepin intoxication is reported. Concentrations of doxepin metabolites and isomers...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2012.08.050

    authors: Neukamm MA,Vogt S,Hermanns-Clausen M,Naue J,Thierauf A,Auwärter V

    更新日期:2013-04-10 00:00:00

  • Early and late meningeal reaction to trauma after long-term brain death.

    abstract::We examined histologically the meninges adherent to traumatic lesions of a patient with brain death sustained for 101 days and observed both early and late reactions of wound healing at the same site of the dura mater. Some parts of the dura mater were thick and histological examinations revealed formation of new vess...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(92)90178-y

    authors: Ito Y,Kimura H

    更新日期:1992-10-01 00:00:00

  • Facial soft tissue thickness in the Brazilian population: new reference data and anatomical landmarks.

    abstract::Facial soft tissue thickness measurement can be useful among several medico-legal techniques that aim to establish the identity of skeletal remains. This study examined the soft tissue thickness that covered the faces of autopsied cadavers sent to the Medico-Legal Institute of Guarulhos from September 2010 to Septembe...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2013.05.024

    authors: de Almeida NH,Michel-Crosato E,de Paiva LA,Biazevic MG

    更新日期:2013-09-10 00:00:00

  • Evaluation of gastrointestinal cancer tissues as a source of genetic information for forensic investigations by using STRs.

    abstract::Malignant tissue samples may sometimes be the only source of biological material for forensic investigations, including identification of individuals or paternity testing. However, in use of such samples, uncertainties due to microsatellite instability (MSI) and loss of heterozygosity (LOH) often associated with neopl...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2003.10.016

    authors: Vauhkonen H,Hedman M,Vauhkonen M,Kataja M,Sipponen P,Sajantila A

    更新日期:2004-01-28 00:00:00

  • Gunshot residue and brakepads: Compositional and morphological considerations for forensic casework.

    abstract::Previous research has raised the possibility that automotive brake pads can produce particles that are both compositionally and morphologically similar to gunshot residue (GSR). These studies, published in the early 2000s, coincided with the reduction or removal of unnecessary sources of lead from the automotive indus...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2016.11.024

    authors: Tucker W,Lucas N,Seyfang KE,Kirkbride KP,Popelka-Filcoff RS

    更新日期:2017-01-01 00:00:00

  • Evaluation of breath-alcohol instruments. IV. Roadside tests with Alcolmeter pocket model.

    abstract::This paper reports results from a field trial with a breath-alcohol screening device--Alcolmeter pocket model. Breath tests were made with drivers apprehended during routine controls (road-blocks), for traffic violations and those involved in traffic accidents. Of 908 roadside breath tests made with chemical reagent t...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(85)90126-4

    authors: Jones AW

    更新日期:1985-08-01 00:00:00

  • Whole human blood DNA degradation associated with artificial ultraviolet and solar radiations as a function of exposure time.

    abstract::Laboratory investigations were conducted to evaluate the effect of ultraviolet radiation components and solar radiation exposure as a function of time on the degradation of whole human blood DNA from the standpoint of forensic analysis. Ten μL of whole human male blood samples were exposed to UV-A, UV-B, UV-C, and sol...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2020.110674

    authors: Rahi GS,Adams JL,Yuan J,Devone DJ,Lodhi KM

    更新日期:2021-02-01 00:00:00

  • An experimental model of death from anaphylactic shock with compound 48/80 and postmortem changes in levels of histamine in blood.

    abstract::The present study was made on an experimental animal model of a death from anaphylaxis, in which postmortem changes in levels of histamine and 1-methylhistamine, in whole blood were measured. Instead of the usual immunological method administering compound 48/80, a degranulating agent of mast cell and the effect close...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(90)90233-o

    authors: Hieda Y,Kageura M,Hara K,Takamoto M,Fukuma Y,Kashimura S

    更新日期:1990-03-01 00:00:00

  • Paraquat myopathy: report on two suicide cases.

    abstract::We describe two suicide cases in which old paraquat was ingested. In conjunction with lung involvement a pronounced degeneration was observed in skeletal muscle of one who died on the 14th day after the ingestion. The following sarcoplasmic or endoplasmic reticulum Ca2+ ATPase (SERCA) monoclonal antibodies were used f...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(98)00190-x

    authors: Tabata N,Morita M,Mimasaka S,Funayama M,Hagiwara T,Abe M

    更新日期:1999-03-15 00:00:00

  • Evaluation of five polymorphic microsatellite markers for typing DNA from decomposed human tissues--correlation between the size of the alleles and that of the template DNA.

    abstract::For PCR-based genotyping using polymorphic microsatellite markers, DNA from decomposed postmortem human tissues was fractionated into six groups according to molecular size. The minimum required amounts of this degraded DNA, for detecting alleles at five microsatellite loci (ACTBP2, CMAG, HUMTH01, CYP19, and LPL) and ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(97)00129-1

    authors: Takahashi M,Kato Y,Mukoyama H,Kanaya H,Kamiyama S

    更新日期:1997-11-10 00:00:00

  • Application of soil in forensic science: residual odor and HRD dogs.

    abstract::Decomposing human remains alter the environment through deposition of various compounds comprised of a variety of chemical constituents. Human remains detection (HRD) dogs are trained to indicate the odor of human remains. Residual odor from previously decomposing human remains may remain in the soil and on surfaces l...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2015.01.025

    authors: Alexander MB,Hodges TK,Bytheway J,Aitkenhead-Peterson JA

    更新日期:2015-04-01 00:00:00

  • Fatal overdose of 2,4-dichlorophenoxyacetic acid (2,4-D).

    abstract::An ingestion of an unknown quantity of U 46 D-Fluid (500 g dichlorophenoxyacetic acid/l) in a suicide is described. Although 2,4-dichlorophenoxyacetic acid (2,4 D) is widely used as a herbicide, intoxications are relatively rare. Quantitation of 2,4-D was performed by diethyl ether extraction from acidified samples (v...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(94)90295-x

    authors: Keller T,Skopp G,Wu M,Aderjan R

    更新日期:1994-03-01 00:00:00

  • Psychopathology and weapon choice: a study of 103 perpetrators of homicide or attempted homicide.

    abstract::The aim of this study was to ascertain if a relationship between weapon choice and psychopathology existed. The perpetrators (103) were evaluated at the Department of Criminology and Forensic Psychiatry of the University of Bari in southern Italy. Psychiatric examination and psycho-diagnostic tests were administered f...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2011.01.019

    authors: Catanesi R,Carabellese F,Troccoli G,Candelli C,Grattagliano I,Solarino B,Fortunato F

    更新日期:2011-06-15 00:00:00

  • A novel approach in personal identification from tissue samples undergone different processes through STR typing.

    abstract::Short tandem repeats (STRs) or microsatellites have been recognized worldwide as a powerful tool for human identification. They have become widely used in human identification especially in criminal cases and mass disasters. Police departments are often interested in cases where tissues are already decomposed and only...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2004.09.052

    authors: Staiti N,Di Martino D,Saravo L

    更新日期:2004-12-02 00:00:00

  • Experimental study of the coupling parameters influencing the terminal effects of thoracic blunt ballistic impacts.

    abstract::The objective of the study is to better understand how blunt projectile ballistic parameters and material properties influence the events leading to injuries. The present work focuses on lateral thoracic impacts and follows an experimental approach. The projectiles are made with a soft foam nose assembled with a rigid...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2015.04.004

    authors: Pavier J,Langlet A,Eches N,Prat N,Bailly P,Jacquet JF

    更新日期:2015-07-01 00:00:00

  • Genetic analysis of the short tandem repeat system D12S391 in the German and three Asian populations.

    abstract::Genomic DNA samples from 222 individuals from Southern China, 154 individuals from Thailand, 100 individuals from Japan as well as from 124 German individuals were analysed for the short tandem repeat (STR) locus D12S391. Typing was carried out by polymerase chain reaction (PCR) amplification and subsequent polyacryra...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(98)00035-8

    authors: Waiyawuth W,Zhang L,Rittner C,Schneider PM

    更新日期:1998-06-08 00:00:00

  • Population data on the 11 STR loci in the Upper Silesia (Poland).

    abstract::Allele frequencies, forensic parameters for the 11 STR loci (TH01, TPOX, CSF1PO, D13S317, D7S820, D16S539, VWA, FESFPS, F13A01, F13B and LPL) were determined in a sample of 140-1262 unrelated adults from the Upper Silesia region (Poland). Comparison of allele frequencies for examined STR loci between the Upper Silesia...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2006.01.011

    authors: Raczek E

    更新日期:2007-05-03 00:00:00

  • Chemical fingerprinting of unevaporated automotive gasoline samples.

    abstract::The comparison of two or more samples of liquid gasoline (petrol) to establish a common origin is a difficult problem in the forensic investigation of arsons and suspicious fires. A total of 35 randomly collected samples of unevaporated gasoline, covering three different grades (regular unleaded, premium unleaded and ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(03)00081-1

    authors: Sandercock PM,Du Pasquier E

    更新日期:2003-06-24 00:00:00

  • The effectiveness of strong afterglow phosphor powder in the detection of fingermarks.

    abstract::There are numerous types of fluorescent fingermark powders or reagents used with the visualization of latent fingermarks deposited on multicolored substrate surfaces that can present a contrast problem if developed with regular fingermark powders. The developed fingermarks can show bright fluorescence upon exposure to...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2008.10.008

    authors: Liu L,Zhang Z,Zhang L,Zhai Y

    更新日期:2009-01-10 00:00:00

  • Pattern of acute poisoning in a medical unit in central Sri Lanka.

    abstract::One hundred and seventy nine cases of acute poisoning constituting 1.1% of all admissions to the medical unit over 3 years were analysed. Eighty-three per cent of the patients were under 30 years of age and 72% were males. The common agents were: OP insecticides in 51%, kerosene oil in 12%, rat poison in 8% and drugs ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(88)90221-6

    authors: Senanayake N,Karalliedde L

    更新日期:1988-01-01 00:00:00

  • Criminal burning.

    abstract::We report a study of 40 burnt bodies on which an autopsy was carried out at the Institut de Médecine Légale in Lyon (28 men/12 women, average age = 41 years, minimum age = 3 years, maximum age = 86 years). Criminal deaths (31%) represented the second cause of death after accidents (52%), and before suicide (16%). Crim...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2005.04.040

    authors: Fanton L,Jdeed K,Tilhet-Coartet S,Malicier D

    更新日期:2006-05-10 00:00:00

  • Effects of temperature and tissue type on Chrysomya rufifacies (Diptera: Calliphoridae) (Macquart) development.

    abstract::The hairy maggot blow fly, Chrysomya rufifacies (Diptera: Calliphoridae), is a forensically important fly often encountered on human and other vertebrate remains in temperate and tropic regions throughout the world including Australia, Asia, Central America and North America. C. rufifacies was reared under controlled ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2014.09.023

    authors: Flores M,Longnecker M,Tomberlin JK

    更新日期:2014-12-01 00:00:00