Abstract:
:We describe a method for obtaining specific and reproducible deoxyribonuclease I (DNase I) typing from liquid semen. Isoelectric focusing of the enzymes on polyacrylamide gel (IEF-PAGE, pH 3.5-5) was accomplished using a 0.5-mm thick gel. The separated isozymes were visualized by a new activity staining method, dried agarose film-overlay (DAFO). Pretreatment of semen samples with neuraminidase markedly enhanced the isozyme-band resolution and sensitivity. The method was simple and reliable, with high resolution and sensitivity. The DNase I types in semen samples were correlated with the types found in corresponding blood and urine samples. DNase I typing could therefore provide an additional discriminant characteristic in the forensic examination of semen.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Sawazaki K,Yasuda T,Nadano D,Tenjo E,Iida R,Takeshita H,Kishi Kdoi
10.1016/0379-0738(92)90043-vkeywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
39-44issue
1eissn
0379-0738issn
1872-6283pii
0379-0738(92)90043-Vjournal_volume
57pub_type
杂志文章abstract::In developed countries, sudden infant death syndrome (SIDS) represents the most prevalent cause of death in children between 1 month and 1 year of age. SIDS is a diagnosis of exclusion, a negative autopsy which requires the absence of structural organ disease. Although investigators have confirmed that a significant p...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.01.018
更新日期:2012-06-10 00:00:00
abstract::Tramadol is an opioid drug metabolised in phase I by cytochrome P450 (CYP) enzymes, of which CYP2D6 is mainly responsible for the O-demethylation of tramadol, but is not involved in N-demethylation. Defects in the genes encoding drug metabolising enzymes (DMEs) may lead to adverse drug effects, even to death. To aid i...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(03)00159-2
更新日期:2003-07-29 00:00:00
abstract::Systematic autopsy was performed in 807 medicolegal deaths where the police had not requested autopsy. In all cases both the cause of death and the mode of death had been reported at the medicolegal external examination. The autopsy revealed differences in the mode of death in about 4% of all cases. This was due in pa...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(80)90190-5
更新日期:1980-01-01 00:00:00
abstract::We investigated 40 patients who had died following open heart surgery between January 1990 and May 1992. Between this time, 703 open heart procedures were carried out at the University Medical School of Debrecen, 2nd Department of Surgery's Cardiac Surgery Unit with a mortality rate of 8.3%. We studied each individual...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(96)01896-8
更新日期:1996-05-31 00:00:00
abstract::Fingerprints present in false identity documents were found on the web. In some cases, laterally reversed (mirrored) images of a same fingerprint were observed in different documents. In the present work, 100 fingerprints images downloaded from the web, as well as their reversals obtained by image editing, were compar...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2016.02.041
更新日期:2016-05-01 00:00:00
abstract:AIMS:The aim of this work is to test the stability of mephedrone added to whole blood collected from alive and dead mephedrone free-users and stored at three different temperatures (-20, +4 and +20°C) with and without preservatives up to 6 months, trying to establish the best storage condition in order to reduce possib...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.07.021
更新日期:2015-11-01 00:00:00
abstract::In bitemark analysis the extent of distortion of both maxillary and mandibular arches and how one affects the other has not been studied. A single dentition was used to create 49 bites on unembalmed cadavers. Landmarks were placed on digital images of the bitemarks and scanned images of the biting dentition. A sample ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.08.044
更新日期:2012-11-30 00:00:00
abstract::The likelihood ratio paradigm has been studied as a means for quantifying the strength of evidence for a variety of forensic evidence types. Although the concept of a likelihood ratio as a comparison of the plausibility of evidence under two propositions (or hypotheses) is straightforward, a number of issues arise whe...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2011.09.013
更新日期:2012-03-10 00:00:00
abstract::The paper extends on the traditional methodology used to quantify DNA evidence in paternity or identification cases. By extending we imply that there are more than two alternatives to choose between. In a standard paternity case the two competing explanations H(1): "John Doe is the father of the child and H(2): "A ran...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00147-x
更新日期:2000-05-08 00:00:00
abstract::In an investigation of postmortem ethanol diffusion deuterium-labeled ethanol-d6 was instilled by peroral gavage immediately after death by CO into the stomach of rat carcasses which were subsequently kept for 12-72 h at 5 or 30 degrees C. The heart blood, abdominal fluid and several tissues were collected and analyze...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(95)01820-4
更新日期:1995-12-29 00:00:00
abstract::The spreading of bloodstains on matt, vinyl silk and gloss painted surfaces, following both perpendicular and non-perpendicular impact, has been studied with a view to understanding whether surface-specific properties affect the size and shape of the final stain. In all cases the dimensions of the stains on the roughe...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.03.044
更新日期:2013-06-10 00:00:00
abstract::Changes related to chronological age are seen in both hard and soft tissue. A number of methods for age estimation have been proposed which can be classified in four categories, namely, clinical, radiological, histological and chemical analysis. In forensic odontology, age estimation based on tooth development is univ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.09.020
更新日期:2014-01-01 00:00:00
abstract::We report a possibly first forensic autopsy case of death following a spontaneous recovery from cardiopulmonary arrest (CPA) after clinical declaration of death: 'Lazarus phenomenon'. A 65-year-old male with congenital deafness and dumbness was found unconscious in his room at a public home. During pre-hospital and cl...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00107-x
更新日期:2002-06-25 00:00:00
abstract::The investigations were carried out on 99 brains of which 83 exhibited macroscopic features of brain oedema. The tissue investigated came from four regions of the brain: the cortex of frontal and occipital regions, the white matter and the tissue of the pons varioli. The percentage of water in these regions was evalua...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(87)90047-8
更新日期:1987-10-01 00:00:00
abstract::Forensic investigation performed on people suspected to be drug abusers covering all Tunisian cities was conducted by monitoring an epidemiological study of human urine samples surveying positive rates of consumption for drugs of abuse. The forensic investigations were conducted on a total of 28,298 arrested individua...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.12.004
更新日期:2018-02-01 00:00:00
abstract::A total of 13 STR loci were typed from over 200 Caucasians from the Strathclyde region in the west of Scotland. ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00317-0
更新日期:2001-02-15 00:00:00
abstract::The population genetic data of 18 X-chromosomal short tandem repeat (STR) markers DXS6807, DXS8378, DXS9895, DXS9902, DXS6810, DXS7132, DXS981, DXS6800, DXS9898, DXS6789, DXS101, DXS6797, GATA172D05, GATA165B12, HPRTB, GATA31E08, DXS8377, and DXS7423 were analyzed in samples of unrelated 220 males and 181 females from...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2004.04.012
更新日期:2005-01-06 00:00:00
abstract::In order to analyze and improve the dental age estimation in children and adolescents for forensic purposes, 22 age estimation methods were compared to a sample of 976 orthopantomographs (662 males, 314 females) of healthy Czech children and adolescents aged between 2.7 and 20.5 years. All methods are compared in term...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.08.005
更新日期:2017-10-01 00:00:00
abstract::The Finnish guidelines for workplace drug testing outlined here represent what is considered the best practice for workplace drug testing to be followed in Finland. The guidelines are based on the act on the protection of privacy in working life (759/2004), the occupational health care act (1383/2001) and the decree o...
journal_title:Forensic science international
pub_type: 指南,杂志文章
doi:10.1016/j.forsciint.2007.03.006
更新日期:2008-01-30 00:00:00
abstract::Based on molecularly imprinted photonic hydrogels (MIPHs) that combined the colloidal-crystal with molecular imprinting technique, a novel label-free colorimetric chemosensor for convenient and fast efficient detection of atropine with high sensitivity and specificity was developed. Due to the special inverse opal arr...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.04.008
更新日期:2013-09-10 00:00:00
abstract::The diffusion and trends in use of each substance is a basic information in policy planning of strategies aiming at deterrence of drug abuse or in the organization of the fight against drug trafficking. The actual diffusion of illicit drugs in a population is hardly measurable, but, among the various measures availabl...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2009.04.018
更新日期:2009-08-10 00:00:00
abstract::Physical fit examinations have long played a critical role in forensic science, particularly in the trace evidence, toolmark, and questioned documents disciplines. Specifically, in trace evidence, physical fits arise in various instances such as separated pieces of duct tape, torn textile fragments, and fractured poly...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110349
更新日期:2020-08-01 00:00:00
abstract::Head hair samples obtained from surgery patients who received fentanyl during anesthesia were analyzed by immunoassay for the presence of fentanyl. Thirteen hair samples were collected from patients following intravenous administration of 1-6 mg of fentanyl. Additional hair samples were collected following the adminis...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(93)90250-e
更新日期:1993-09-01 00:00:00
abstract::A multicentre retrospective analysis of 4450 autopsies carried out due to suspicion of medical malpractice in 17 German institutes of forensic medicine from 1990 to 2000 was performed for the German Federal Ministry of Health. During the time period analysed an increase of cases could be mentioned. The main results of...
journal_title:Forensic science international
pub_type: 杂志文章,多中心研究
doi:10.1016/j.forsciint.2009.05.013
更新日期:2009-09-10 00:00:00
abstract:INTRODUCTION:Ethanol and heroin are both depressant drugs on the central nervous system, and combined use is known to be dangerous due to pharmacodynamic interactions, leading to an even higher risk of respiratory depression and death. In addition, previous studies have suggested a pharmacokinetic interaction between e...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.06.032
更新日期:2014-09-01 00:00:00
abstract::Forensic investigators commonly interpret bone fracture patterns to estimate the force required to generate that trauma. Unfortunately, these estimates are limited to qualitative values such as "mild", "moderate" or "extreme" force. This work presents a new experimental forensic device developed to simulate blunt- and...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.110123
更新日期:2020-02-01 00:00:00
abstract::An improved technique for cocaine extraction from urine samples for gas chromatographic (GC) analysis is described. Employing a simple liquid-liquid extraction (LLE) of cocaine with a mixture of ethyl ether:isopropanol (9:1) the method presents a mean recovery of 74.49%. Limit of detection (LOD) and limit of quantific...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00124-x
更新日期:2002-07-17 00:00:00
abstract::This paper describes the development and validation of a method for the analysis of office papers by measuring carbon isotopes using isotope ratio mass spectrometry (IRMS). The method development phase included testing protocols for storage, sample materials, set-up of the analytical run; and examining the effects of ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.05.009
更新日期:2013-09-10 00:00:00
abstract::Following the introduction of limit values for blood alcohol and breath alcohol concentrations of 0.5 g/kg and 0.25 mg/L, respectively, as provided under s. 24 a of the German Road Traffic Act the question is whether also breath alcohol concentrations can be back calculated to the time of the traffic offence in cases ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2004.03.023
更新日期:2004-07-16 00:00:00
abstract::Little is currently known about the importance of clotting during the drying of blood pools. While this is of little moment for droplets where drying occurs faster than contact-phase-induced clotting, clotting may significantly influence blood pools drying as it transform a liquid into a gel. To investigate this influ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.110008
更新日期:2019-12-01 00:00:00