A new individualization marker of semen: deoxyribonuclease I (DNase I) polymorphism.

Abstract:

:We describe a method for obtaining specific and reproducible deoxyribonuclease I (DNase I) typing from liquid semen. Isoelectric focusing of the enzymes on polyacrylamide gel (IEF-PAGE, pH 3.5-5) was accomplished using a 0.5-mm thick gel. The separated isozymes were visualized by a new activity staining method, dried agarose film-overlay (DAFO). Pretreatment of semen samples with neuraminidase markedly enhanced the isozyme-band resolution and sensitivity. The method was simple and reliable, with high resolution and sensitivity. The DNase I types in semen samples were correlated with the types found in corresponding blood and urine samples. DNase I typing could therefore provide an additional discriminant characteristic in the forensic examination of semen.

journal_name

Forensic Sci Int

authors

Sawazaki K,Yasuda T,Nadano D,Tenjo E,Iida R,Takeshita H,Kishi K

doi

10.1016/0379-0738(92)90043-v

keywords:

subject

Has Abstract

pub_date

1992-11-01 00:00:00

pages

39-44

issue

1

eissn

0379-0738

issn

1872-6283

pii

0379-0738(92)90043-V

journal_volume

57

pub_type

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