Age estimation using a modified HPLC determination of ratio of aspartic acid in dentin.

Abstract:

:Diastereomeric dipeptides were derived from the amino acid enantiomers in dentin by O-phthalaldehyde-N-acetyl-L-cysteine. The products were separated using high performance liquid chromatography (HPLC) and detected by fluorescence detector. A short analysis time (total analysis time was 15 min, including retention time, sample derivatization time and column regeneration time) was used. The sensitivity of detection was 1 pmol and high resolution (Rs = 1.5) was reached. We determined the D/L ratio of aspartic acid in dentin of 28 first premolars. The correlation value between the D/L ratio of aspartic acid and actual age was 0.9887; errors of +/- 1 year accounted for 46.4%, and no error exceeded 5 years.

journal_name

Forensic Sci Int

authors

Fu SJ,Fan CC,Song HW,Wei FQ

doi

10.1016/0379-0738(95)01709-7

subject

Has Abstract

pub_date

1995-05-09 00:00:00

pages

35-40

issue

1

eissn

0379-0738

issn

1872-6283

pii

0379-0738(95)01709-7

journal_volume

73

pub_type

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