Genotyping for DQA1 and PM loci in urine using PCR-based amplification: effects of sample volume, storage temperature, preservatives, and aging on DNA extraction and typing.

Abstract:

:Urine is often the sample of choice for drug screening in aviation/general forensic toxicology and in workplace drug testing. In some instances, the origin of the submitted samples may be challenged because of the medicolegal and socioeconomic consequences of a positive drug test. Methods for individualization of biological samples have reached a new boundary with the application of the polymerase chain reaction (PCR) in DNA profiling, but a successful characterization of the urine specimens depends on the quantity and quality of DNA present in the samples. Therefore, the present study investigated the influence of storage conditions, sample volume, concentration modes, extraction procedures, and chemical preservations on the quantity of DNA recovered, as well as the success rate of PCR-based genotyping for DQA1 and PM loci in urine. Urine specimens from male and female volunteers were divided and stored at various temperatures for up to 30 days. The results suggested that sample purification by dialfiltration, using 3000-100,000 molecular weight cut-off filters, did not enhance DNA recovery and typing rate as compared with simple centrifugation procedures. Extraction of urinary DNA by the organic method and by the resin method gave comparable typing results. Larger sample volume yielded a higher amount of DNA, but the typing rates were not affected for sample volumes between 1 and 5 ml. The quantifiable amounts of DNA present were found to be greater in female (14-200 ng/ml) than in male (4-60 ng/ml) samples and decreased with the elapsed time under both room temperature (RT) and frozen storage. Typing of the male samples also demonstrated that RT storage samples produced significantly higher success rates than that of frozen samples, while there was only marginal difference in the DNA typing rates among the conditions tested using female samples. Successful assignment of DQA1 + PM genotype was achieved for all samples of fresh urine, independent of gender, starting sample volume, or concentration method. Preservation by 0.25% sodium azide was acceptable for sample storage at 4 degrees C during a period of 30 days. For longer storage duration, freezing at -70 degrees C may be more appropriate. Thus, the applicability of the DQA1 + PM typing was clearly demonstrated for individualization of urine samples.

journal_name

Forensic Sci Int

authors

Vu NT,Chaturvedi AK,Canfield DV

doi

10.1016/s0379-0738(99)00034-1

keywords:

subject

Has Abstract

pub_date

1999-05-31 00:00:00

pages

23-34

issue

1

eissn

0379-0738

issn

1872-6283

pii

S0379-0738(99)00034-1

journal_volume

102

pub_type

杂志文章
  • The history of child abuse.

    abstract::Though the child abuse syndrome is usually considered to have been first described in the middle of the present century, references to child abuse occur throughout history and in the 19th century, detailed descriptions were published in the French medical literature. ...

    journal_title:Forensic science international

    pub_type: 历史文章,杂志文章

    doi:10.1016/0379-0738(86)90008-3

    authors: Knight B

    更新日期:1986-02-01 00:00:00

  • Gas chromatographic examination of postmortem specimens after maprotiline intoxication.

    abstract::Ingestion of an unknown quantity of Ludiomil (maprotiline)-costed tablets in a suicide is described. Although maprotiline is known for over 20 years now, relatively few cases of intoxications due to maprotiline overdose have been reported. The authors report a new and quick method to analyze and determine maprotiline ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(97)00064-9

    authors: Keller T,Zollinger U

    更新日期:1997-08-04 00:00:00

  • Method validation of a survey of thevetia cardiac glycosides in serum samples.

    abstract::A sensitive and specific liquid chromatography tandem mass spectrometry (HPLC-ESI(+)-MS/MS) procedure was developed and validated for the identification and quantification of thevetin B and further cardiac glycosides in human serum. The seeds of Yellow Oleander (Thevetia peruviana) contain cardiac glycosides that can ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2011.02.013

    authors: Kohls S,Scholz-Böttcher B,Rullkötter J,Teske J

    更新日期:2012-02-10 00:00:00

  • Pathogenesis of heroin-associated glomerulonephritis. Correlation between the inflammatory activity and renal deposits of immunoglobulin and complement?

    abstract::Renal specimens from 19 autopsies of persons known to be intravenous heroin-addicts and with severe lympho-monocytic glomerulonephritis were investigated to correlate the inflammatory activity and deposits of immunoglobulin and complement. In all sections, there were 10 or more LCA-positive cells/glomerulum, counted i...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(00)00214-0

    authors: Dettmeyer R,Stojanovski G,Madea B

    更新日期:2000-09-11 00:00:00

  • Empirical evaluation of spring powered air rifle storage and modifications on forensic practice and casework.

    abstract::Air weapons are commonly used by civilian populations across the world, particularly by those under 18, and discharges often result in desecration, criminal damage and animal abuse. Online forums and websites provide an accessible resource for civilians to access airgun modification methods proposing to increase muzzl...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2018.11.004

    authors: Greenslade KJ,Bolton-King RS

    更新日期:2019-01-01 00:00:00

  • Canine human scent identifications with post-blast debris collected from improvised explosive devices.

    abstract::In this study it is demonstrated that human odor collected from items recovered at a post-blast scene can be evaluated using human scent specific canine teams to locate and identify individuals who have been in contact with the improvised explosive device (IED) components and/or the delivery vehicle. The purpose of th...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2010.03.021

    authors: Curran AM,Prada PA,Furton KG

    更新日期:2010-06-15 00:00:00

  • Analysis of gunshot residues as trace in nasal mucus by GFAAS.

    abstract::When a gun is fired, the majority of gunshot residues are deposited on the shooter's hands. But these residues disappear through contact with surfaces or washing. Therefore, the maximum time frame to find GSR on a suspect's hands is 8h. The mucus, inside of a nostril, forms a surface layer where they are trapped forei...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2016.01.034

    authors: Aliste M,Chávez LG

    更新日期:2016-04-01 00:00:00

  • Case histories in forensic medicine.

    abstract::Case histories make up a great part of publications in forensic medicine, especially forensic pathology, although considered low on the hierarchy of evidence based medicine because of its inherent anecdotal nature. Meanwhile some journals exclude case histories categorically from publication. The boom of case historie...

    journal_title:Forensic science international

    pub_type: 杂志文章,评审

    doi:10.1016/j.forsciint.2006.05.012

    authors: Madea B

    更新日期:2007-01-17 00:00:00

  • Haemorrhages in head tissues during the asphyxiation process.

    abstract::Two rates of carbon dioxide flow into the termination chamber were applied for the euthanasia of rats. The classical phases of asphyxia were observed in both groups of animals although their course was prolonged in rats exposed to the lower gas flow. The haemorrhages were assessed histologically in the following struc...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(01)00591-6

    authors: Hauser R,Jankowski Z,Gos T,Krzyzanowski M

    更新日期:2001-12-27 00:00:00

  • Guidelines for international forensic bio-archaeology monitors of mass grave exhumations.

    abstract::The systematic exhumation of mass graves is becoming a frequent occurrence globally. Historically, it has been mostly anthropologists with their particular expertise in osteology that have been engaged by non-governmental organizations (NGO) to monitor mass grave exhumations and postmortem examinations, conducted by a...

    journal_title:Forensic science international

    pub_type: 指南,杂志文章

    doi:10.1016/s0379-0738(03)00124-5

    authors: Skinner M,Alempijevic D,Djuric-Srejic M

    更新日期:2003-07-08 00:00:00

  • Postmortem redistribution of Δ9-tetrahydrocannabinol (THC), 11-hydroxy-THC (11-OH-THC), and 11-nor-9-carboxy-THC (THCCOOH).

    abstract:INTRODUCTION:Postmortem redistribution (PMR), a well-described phenomenon in forensic toxicology for certain drugs, can result in increased central blood concentrations relative to peripheral blood concentrations. Δ(9)-tetrahydrocannabinol (THC), the primary psychoactive component in cannabis or marijuana, is the illic...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2011.06.028

    authors: Holland MG,Schwope DM,Stoppacher R,Gillen SB,Huestis MA

    更新日期:2011-10-10 00:00:00

  • The history of forensic medicine in times of the Weimar republic and national socialism--an approach.

    abstract::German Forensic Medicine established the tasks and aims of forensic medicine during the period of the "Weimar Republic" (1919-1933). Since 1925, forensic medicine is part of the medical examination regulation. In times of the 3rd Reich (1933-1945), most of forensic pathologists were influenced by and involved in Natio...

    journal_title:Forensic science international

    pub_type: 历史文章,杂志文章

    doi:10.1016/j.forsciint.2004.04.044

    authors: Lignitz E

    更新日期:2004-09-10 00:00:00

  • Value of glycolic acid analysis in ethylene glycol poisoning: A clinical case report and systematic review of the literature.

    abstract:OBJECTIVE:To evaluate the clinical utility of glycolic acid (GA) determination in the diagnosis and prognosis of ethylene glycol (EG) intoxications. METHOD:Systematic review of serum and/or urine GA concentrations available in the literature in cases of EG poisoning. Present a clinical case in which the determination ...

    journal_title:Forensic science international

    pub_type: 杂志文章,评审

    doi:10.1016/j.forsciint.2018.07.007

    authors: Tuero G,González J,Sahuquillo L,Freixa A,Gomila I,Elorza MÁ,Barceló B

    更新日期:2018-09-01 00:00:00

  • Molecular pathology of pulmonary edema in forensic autopsy cases with special regard to fatal hyperthermia and hypothermia.

    abstract::Fatalities due to an extreme ambient temperature might present with poor or nonspecific pathologies; thus, the diagnosis of the cause of death in such cases is one of the most difficult tasks in forensic pathology. The present study investigated the molecular pathology of alveolar damage involving pulmonary edema with...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2013.03.007

    authors: Wang Q,Ishikawa T,Michiue T,Zhu BL,Guan DW,Maeda H

    更新日期:2013-05-10 00:00:00

  • Weapon identification using antemortem computed tomography with virtual 3D and rapid prototype modeling--a report in a case of blunt force head injury.

    abstract::A frequent request of a prosecutor referring to forensic autopsy is to determine the mechanism of an injury and to identify the weapons used to cause those injuries. This task could be problematic in many ways, including changes in the primary injury caused by medical intervention and the process of healing. To accomp...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2012.06.012

    authors: Woźniak K,Rzepecka-Woźniak E,Moskała A,Pohl J,Latacz K,Dybała B

    更新日期:2012-10-10 00:00:00

  • Suspected suicide and suicide attempt with mysterious concomitant circumstances.

    abstract::Two cases of suicide and attempted suicide, respectively, are presented. A 15-year old boy suffering from diabetes died of hyperglycemia after continuous drinking of large amounts of sugared tea. He concealed his disease from the teachers as well as from the doctor. A 52-year old woman was found with multiple blunt fo...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2004.09.087

    authors: Darok M,Gatternig R

    更新日期:2005-01-17 00:00:00

  • Genetic data on 19 STR loci in south-east Poland.

    abstract::Allele frequencies for the 19 STRs loci, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, F13A01, F13B, FESFPS, FGA, LPL, Penta D, Penta E, TH01, TPOX and VWA were obtained from a sample of 203-1188 unrelated individuals living in the area of south-east Poland. ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2003.10.001

    authors: Kozioł P,Ciesielka M,Madro R,Krajka A

    更新日期:2004-01-06 00:00:00

  • Toxicological screen on gastric aspirates: the Singapore experience.

    abstract::In the early eighties, in response to increasing self-poisoning incidents by pharmaceuticals in Singapore, a comprehensive wide-sweep screen for these drugs in clinical gastric aspirates was devised and implemented by us. The toxicological screen which involved quadruplicate extractions, two in acidic conditions and t...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(92)90158-s

    authors: Chao TC,Lo DS,Gunasegaram R,Tan-Siew WF,Tay MM

    更新日期:1992-05-01 00:00:00

  • Drug abuse in western Denmark during the eighties. I. Drugs of abuse.

    abstract::A description is given of the availability and quality of drugs seized in the Danish illicit drug market during the eighties. Cannabis was the most prevalent illegal drug throughout the decade. Seizures of heroin predominated among the 'hard' illegal drugs until 1987 but during the late eighties a significant increase...

    journal_title:Forensic science international

    pub_type: 历史文章,杂志文章

    doi:10.1016/0379-0738(92)90095-e

    authors: Kaa E

    更新日期:1992-07-01 00:00:00

  • Accuracy of developing tooth length as an estimate of age in human skeletal remains: the deciduous dentition.

    abstract::Dental age assessments are widely used to estimate age of immature skeletal remains. Most methods have relied on fractional stages of tooth emergence and formation, particularly of the permanent dentition, for predicting the age of infants and very young children. In this study, the accuracy of regression equations of...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2006.11.006

    authors: Cardoso HF

    更新日期:2007-10-02 00:00:00

  • A comparison of efficiency of manual and automatic fibres search with the Maxcan fibre finder.

    abstract::The aim of this work was to study the efficiency of automatic fibre searching with the Maxcan fibre finder (Cox Analytical Systems, Sweden) in comparison to manual searching. The influence of some parameters (color, thickness, background noise) on the results of a fibre search was considered. Eighteen experimental tap...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2005.08.015

    authors: Monard Sermier F,Massonnet G,Buzzini P,Fortini A,Gason F,De Wael K,Rovas P

    更新日期:2006-07-13 00:00:00

  • 1H and 31P benchtop NMR of liquids and solids used in and/or produced during the manufacture of methamphetamine by the HI reduction of pseudoephedrine/ephedrine.

    abstract::In this study, the use of benchtop NMR spectroscopy in the analysis of solids and liquids used and/or produced during the HI reduction of pseudoephedrine was evaluated. The study focused on identifying organic precursors and phosphorus containing compounds used in and/or produced during the manufacturing process. Samp...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2017.06.026

    authors: Bogun B,Moore S

    更新日期:2017-09-01 00:00:00

  • Generalized arterial calcification of infancy--Findings at post-mortem computed tomography and autopsy.

    abstract::Generalized arterial calcification in infancy is a rare genetic disorder characterized by abnormal calcification of large and medium sized arteries and marked myointimal proliferation resulting in arterial stenosis. The condition is often fatal secondary to complications of cardiac ischemia, hypertension and cardiac f...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2015.06.017

    authors: Bolster F,Ali Z,Southall P,Fowler D

    更新日期:2015-09-01 00:00:00

  • Individualizing characteristics of footprints in Gujjars of North India--forensic aspects.

    abstract::Careful examination of foot impressions can provide important evidences and clues in a crime scene investigation. The present study is conducted on a cross-sectional sample of 1040 adult male Gujjars inhabiting the sub-Himalayan region of North India. The study describes the utility of individualizing characteristics ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2006.08.006

    authors: Krishan K

    更新日期:2007-07-04 00:00:00

  • HIV-1 prevalence among drug deaths--a multicenter study.

    abstract::Since 1985 several European Institutes of Forensic Medicine have cooperated in a multicenter study providing a constant monitoring of HIV-1 prevalence among drug-related deaths. From 1985 to 1991 the HIV status of 5372 drug-related fatalities (4328 males, 1044 females) has been tested. A total of 764 (14%) drug deaths...

    journal_title:Forensic science international

    pub_type: 杂志文章,多中心研究

    doi:10.1016/0379-0738(93)90050-k

    authors: Lockemann U,Püschel K

    更新日期:1993-11-01 00:00:00

  • Toenails as an alternative source material for the extraction of DNA from decomposed human remains.

    abstract::The DNA identification of decomposed human remains for coronial investigations at the Victorian Institute of Forensic Medicine routinely requires the retrieval and processing of a bone sample obtained from the deceased. Bone is a difficult sample type to work with as it requires surgical removal from the deceased, ref...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2015.10.025

    authors: Schlenker A,Grimble K,Azim A,Owen R,Hartman D

    更新日期:2016-01-01 00:00:00

  • Determination of polymorphism at eight STR loci in the Croatian population using automated detection.

    abstract::Population study was carried out on the sample of 167 unrelated donors from the wider area of the Croatia's capital, Zagreb, using the short tandem reapet (STR) loci: TH01, VWFA31, FES/FPS, F13A01, D1S1656, D12S391, D18S535 and D22S683. ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(02)00096-8

    authors: Grubić Z,Stingl K,Kastelan A

    更新日期:2002-06-25 00:00:00

  • Functional characterization of SCN10A variants in several cases of sudden unexplained death.

    abstract:BACKGROUND:Multiple genome-wide association studies (GWAS) and targeted gene sequencing have identified common variants in SCN10A in cases of PR and QRS duration abnormalities, atrial fibrillation and Brugada syndrome. The New York City Office of Chief Medical Examiner has now also identified five SCN10A variants of un...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2019.05.042

    authors: Gando I,Williams N,Fishman GI,Sampson BA,Tang Y,Coetzee WA

    更新日期:2019-08-01 00:00:00

  • The effect of clothing on decomposition and vertebrate scavengers in cooler months of the temperate southwestern Cape, South Africa.

    abstract::With research providing conflicting results from different habitats across the globe, the effect of clothing on decomposition is unclear; some studies indicate clothing increases decomposition rate by facilitating increased insect activity, and others conclude clothing prolongs the decay process. In South Africa, such...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2020.110197

    authors: Spies MJ,Finaughty DA,Friedling LJ,Gibbon VE

    更新日期:2020-04-01 00:00:00

  • Quantification of spheno-occipital synchondrosis fusion in a contemporary Malaysian population.

    abstract::Timing of fusion of the spheno-occipital synchondrosis (SOS) is correlated with age. Previous research, however, has demonstrated variation in the timing of closure among different global populations. The present study aims to quantify the timing of SOS fusion in Malaysian individuals as visualised in multi-detector c...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2017.12.046

    authors: Hisham S,Flavel A,Abdullah N,Noor MHM,Franklin D

    更新日期:2018-03-01 00:00:00