Abstract:
:The Purkinje-cells (PCs) of the cerebellum are highly vulnerable to hypoxic/ischemic insult. Calbindin-D28k is a calcium-binding protein that is strongly expressed in PCs. Following hypoxia, a decrease in its concentration has been found in animal models before any morphological change of the PCs took place. Hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) are increasingly expressed in tissues that undergo durations of hypoxia, and also in brain tissues. We examine whether a change in expression of any of these factors, or a combination of alterations, is an indicator of acute hypoxia. We investigated the intensity of neuronal immunoreactivity of calbindin-D28k, HIF-1α and VEGF retrospectively in 141 samples of human cerebellar tissue obtained from autopsies performed at the Institute of Legal Medicine of Hanover Medical School in 2007 and 2008. Three groups were compared. The first group comprises individuals (n=48) who died due to acute hypoxia, such as drowning or asphyxia. The second is a control group comprising individuals who died due to heart failure (n=56), and the third group comprised individuals who died almost instantly of polytraumata (n=37). Our study finds a statistically significant decrease in the expression of calbindin-D28k (p<0.05) in PCs in the acute hypoxia group, relative to the control groups. No changes in the expression of HIF-1α or in the expression of VEGF were observed in any of the groups. Consequently HIF-1α and VEGF were not suitable indicators, whereas detection of a decreasing concentration of calbindin-D28k supports a diagnosis of acute hypoxia.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Bartschat S,Fieguth A,Könemann J,Schmidt A,Bode-Jänisch Sdoi
10.1016/j.forsciint.2012.08.023subject
Has Abstractpub_date
2012-11-30 00:00:00pages
165-70issue
1-3eissn
0379-0738issn
1872-6283pii
S0379-0738(12)00386-6journal_volume
223pub_type
杂志文章abstract::A large number of passerine birds, mainly greenfinches, were found dead or dying in a hedgerow close to a field of onions recently sprayed with sodium monochloroacetate (SMCA). An analytical method is described for isolating monochloroacetic acid from bird tissues, as its potassium salt, by ion exchange chromatography...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(92)90075-8
更新日期:1992-04-01 00:00:00
abstract::The experience of the Northern Ireland State Pathology Service is drawn on to illustrate the forensic aspects of death by shooting and bombing caused by terrorists. The investigations into these deaths have to contend with rumours and allegations which can inflame feelings in the community or mislead the investigators...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(88)90215-0
更新日期:1988-01-01 00:00:00
abstract::This study determines the development rates of the immature stages of the fly Ophyra albuquerquei Lopes, 1985. In this study, with 70% relative humidity and a 12-h photophase, eggs, larvae, and pupae were kept in a biochemical oxygen demand chamber at temperatures of 10, 15, 17, 20, 22, 25, 27, 30, 33, 35, and 40°C. W...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.07.014
更新日期:2015-09-01 00:00:00
abstract::The rate of suicides in road traffic fatalities is estimated at 1-8% depending on the investigated study material. Especially single-car accidents, for which no technical reason or driving error is apparent, are considered suspicious. The elucidation of suicides in road traffic is difficult if there are no relevant cl...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2010.11.023
更新日期:2011-04-15 00:00:00
abstract::Most of the automotive companies use cast iron for their engine blocks. Restoration of obliterated number on these iron surfaces by chemical etching is known to be quite difficult. Heating of the obliterated surface using oxyacetylene flame is an alternative recovery treatment suggested in literature and used in pract...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.07.020
更新日期:2012-11-30 00:00:00
abstract::In this work, a case of deliberate self-poisoning is presented. A 14-year-old girl suddenly died during one of the several hospitalizations. Abdominal computer tomography showed a large number of metallic particles in the large intestine. Analysis of blood and internal organs for mercury and other toxic metals carried...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.02.015
更新日期:2014-04-01 00:00:00
abstract::We describe a method for obtaining specific and reproducible deoxyribonuclease I (DNase I) typing from liquid semen. Isoelectric focusing of the enzymes on polyacrylamide gel (IEF-PAGE, pH 3.5-5) was accomplished using a 0.5-mm thick gel. The separated isozymes were visualized by a new activity staining method, dried ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(92)90043-v
更新日期:1992-11-01 00:00:00
abstract::In January 2015, 75 people died and 177 were hospitalized in the Mozambique village of Chitima after attending a funeral. The deaths were linked to the consumption of a traditional African beverage called pombe. Samples of the suspect pombe were subjected to myriad analyses and compared to a control sample. Ultimately...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2016.10.015
更新日期:2017-01-01 00:00:00
abstract::The establishment of approaches for the differentiation of the ink entries of seals on paper can provide evidence to authenticate the related documents and can play a key role in judicial expertise. The identification and discrimination method for 38 red ink entries of seals on paper has been investigated using laser ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.12.034
更新日期:2014-03-01 00:00:00
abstract::In a medicolegal study the postmortem serotonin (5-HT) cerebrospinal fluid (CSF) concentrations were determined in routine autopsies using a high performance liquid chromatographic procedure with electrochemical detection. There was no correlation between 5-HT concentrations and age, sex or blood alcohol concentration...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2004.02.023
更新日期:2004-06-10 00:00:00
abstract::Hair samples of 20 volunteers of the techno-music scene, who more or less regularly consumed ecstasy tablets and speed and anonymously reported their abuse history, were analyzed in one to seven 3 cm segments for amphetamine (A), methamphetamine (MA), methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDM...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(97)00123-0
更新日期:1997-09-19 00:00:00
abstract::In the field of questioned document examinations, the disadvantages of qualitative examination based on expert experience are becoming more and more obvious. In the source determination of laser-printed documents, the quantitative evaluation based on image quality is more and more important. In this paper, the ImageXp...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110603
更新日期:2021-01-01 00:00:00
abstract::A population study on the D1S80 locus in 100 northern Thais was performed using polymerase chain reaction (PCR) and high resolution polyacrylamide gel electrophoresis. Sixteen alleles and 39 genotypes were observed with a heterozygosity of 82%. In contrast to other studies, the allele with 31 repeat units was found to...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(96)01960-3
更新日期:1996-08-15 00:00:00
abstract::During the past decade, several studies have been carried out using the calcaneus bone for sex estimation. This paper collected data using ten variables for metric characteristics of the calcaneus of 144 modern Cretans and examined their correlation with known sex. Secondly, the formulae developed by Peckmann et al. (...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.04.005
更新日期:2017-08-01 00:00:00
abstract::A single nucleotide polymorphism (SNP) multiplex has been developed to analyse highly degraded and low copy number (LCN) DNA template, i.e. <100 pg, for scenarios including mass disaster identification. The multiplex consists of 20 autosomal non-coding loci plus Amelogenin for sex determination, amplified in a single ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2004.12.011
更新日期:2005-11-10 00:00:00
abstract::The aim of the study was to describe victim, assailant, assault characteristics for sexual assault victims according to the time between the last sexual assault and the examination, and to provide descriptive data on medico-legal findings. The study was based on 418 examined victims of sexual assault during the year 1...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00427-9
更新日期:2003-01-28 00:00:00
abstract::In literature, cases of nutmeg abuse have been described repeatedly, but only one fatal case of poisoning was reported [1]. In the present case, myristicin (4 microg/ml) was detected for the first time in the postmortal serum of a 55-year-old woman. Identification was achieved with the aid of UV-VIS spectroscopy and T...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00369-8
更新日期:2001-04-15 00:00:00
abstract::A number of biological and chemical tests have been developed over the years to determine whether a person was drowned. This study focuses on the potential of a microbiological test for detecting common bacterial markers of water faecal pollution such as faecal coliforms (FC) and faecal streptococci (FS) as possible i...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2008.09.004
更新日期:2008-11-20 00:00:00
abstract::Whole exome sequencing (WES) and bioinformatics analysis were used to investigate potential disease-causing gene mutations in a sudden unexplained death syndrome (SUDS) case after autopsy and pathology tests failed to suggest an obvious disease mechanism. Following whole exome sequencing, a 3-step bioinformatics filte...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.08.022
更新日期:2015-12-01 00:00:00
abstract:AIM:To highlight some problems that may occur when investigating clozapine-associated deaths including (i) that death may be related to gastrointestinal hypomotility and (ii) that post-mortem blood clozapine and norclozapine concentrations may not reflect ante-mortem concentrations. CASE REPORTS:A 41-year-old male die...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2010.07.021
更新日期:2011-03-20 00:00:00
abstract::Desomorphine is reported to be the principal pharmacologically active opioid in Krokodil, a homemade injectable drug that is perceived to be a cheaper alternative to heroin. There have been limited studies regarding its pharmacology or detection in biological matrices. The goal of this study was to contribute further ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.05.017
更新日期:2018-08-01 00:00:00
abstract::The objective of the present work was to compare previously published methods and provide validation data to detect simultaneously cocaine (COC), benzoylecgonine (BE) and norcocaine (NCOC) in nail. Finger and toenail samples (5mg) were cut in very small pieces and submitted to an initial procedure for external deconta...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2005.07.021
更新日期:2006-06-02 00:00:00
abstract::During a 3-year period (August 1st, 1985 to July 31st, 1988) a systematic investigation of medico-legal autopsy cases with regard to the presence of antibodies for HIV-virus was carried out at the Department of Forensic Medicine in Stockholm, Sweden. Prior to autopsy, blood samples were taken from femoral or subclavia...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(89)90220-x
更新日期:1989-06-01 00:00:00
abstract::Traditionally, sperms are isolated from vaginal cell mixtures by preferential extraction methods. Although these methods work well when there is a reasonable amount of DNA present, they are problematic when there are limited amounts (ca. 250 pg). In particular, the analysis of sperm from microscope slides has proven d...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(03)00267-6
更新日期:2003-10-14 00:00:00
abstract::As well as being the route for gaseous exchange, the nasal passages protect the lungs from debris and fine particulates inhaled during breathing. Foreign particles are trapped by mucus produced by membranes that line the upper respiratory tract. Even when a corpse has skeletonised, particles can be retrieved from the ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2005.11.019
更新日期:2006-11-22 00:00:00
abstract::Organic impurities of methamphetamine may show different patterns by synthesis. In the present study, we tried to find the impurities reflecting the conditions of synthesis by comparing impurity patterns of the methamphetamine samples synthesized by different methods. Sixteen methamphetamine samples were synthesized f...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.02.054
更新日期:2006-09-12 00:00:00
abstract::Myxoid heart disease (MHD), more commonly known as mitral valve prolapse, is a very common cardiac abnormality affecting 5-10% of the general population. This article reviews the history, symptoms, physical findings, pathology, associated conditions and complications of this entity. Special emphasis is given to sudden...
journal_title:Forensic science international
pub_type: 杂志文章,评审
doi:10.1016/0379-0738(89)90177-1
更新日期:1989-03-01 00:00:00
abstract::We report a possibly first forensic autopsy case of death following a spontaneous recovery from cardiopulmonary arrest (CPA) after clinical declaration of death: 'Lazarus phenomenon'. A 65-year-old male with congenital deafness and dumbness was found unconscious in his room at a public home. During pre-hospital and cl...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00107-x
更新日期:2002-06-25 00:00:00
abstract::Allele frequencies for the 15 STRs included in the AmpFlSTR SGM Plus and Profiler kit (D3S1358, VWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, THO1, FGA, TPOX, CSF1PO, D5S818, D13S317 and D7S820) were obtained from a sample of 597 unrelated individuals living in Taiwan. All loci, except for D8S1179 and D5S81...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2003.09.006
更新日期:2003-12-17 00:00:00
abstract::A sample of 166 normal adult males and females was taken from different colleges of Assiut representing those living in upper Egypt. Each subject has been studied for measurements of stature (S), hand length (HL) and hand breadth (HB). The data were statistically analysed in order to assess the relationship between st...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(90)90304-h
更新日期:1990-07-01 00:00:00