Abstract：
:The objective of the present work was to compare previously published methods and provide validation data to detect simultaneously cocaine (COC), benzoylecgonine (BE) and norcocaine (NCOC) in nail. Finger and toenail samples (5mg) were cut in very small pieces and submitted to an initial procedure for external decontamination. Methanol (3 ml) was used to release analytes from the matrix. A cleanup step was performed simultaneously by solid-phase extraction (SPE) and the residue was derivatized with pentafluoropropionic anhydride/pentafluoropropanol (PFPA/PFP). Gas chromatography-mass spectrometry (GC-MS) was used to detect the analytes in selected ion monitoring mode (SIM). Confidence parameters of validation of the method were: recovery, intra- and inter-assay precision, as well as limit of detection (LOD) of the analytes. The limits of detection were: 3.5 ng/mg for NCOC and 3.0 ng/mg for COC and BE. Good intra-assay precision was observed for all detected substances (coefficient of variation (CV)<11%). The inter-assay precision for norcocaine and benzoylecgonine were <4%. For intra- and inter-assay precision deuterated internal standards were used. Toenail and fingernail samples from eight declared cocaine users were submitted to the validated method.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Valente-Campos S,Yonamine M,de Moraes Moreau RL,Silva OAdoi
10.1016/j.forsciint.2005.07.021keywords:
subject
Has Abstractpub_date
2006-06-02 00:00:00pages
218-22issue
2-3eissn
0379-0738issn
1872-6283pii
S0379-0738(05)00451-2journal_volume
159pub_type
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journal_title：Forensic science international
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pub_type： 杂志文章
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journal_title：Forensic science international
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journal_title：Forensic science international
pub_type： 杂志文章
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journal_title：Forensic science international
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