Abstract：
:A single nucleotide polymorphism (SNP) multiplex has been developed to analyse highly degraded and low copy number (LCN) DNA template, i.e. <100 pg, for scenarios including mass disaster identification. The multiplex consists of 20 autosomal non-coding loci plus Amelogenin for sex determination, amplified in a single tube PCR reaction and visualised on the Applied Biosystems 3100 capillary electrophoresis (CE) system. Allele-specific primers tailed with shared universal tag sequences were designed to speed multiplex design and balance the amplification efficiencies of all loci through the use of a single reverse and two differentially labelled allele denoting forward universal primers. As the multiplex is intended for use with samples too degraded for conventional profiling, a computer program was specifically developed to aid interpretation. Critical factors taken into account by the software include empirically determined extremes of heterozygous imbalance (Hb) and the drop-out threshold (Ht) defined as the maximum peak height of a surviving heterozygous allele, where its partner may have dropped out. The discrimination power of the system is estimated at 1 in 4.5 million, using a White Caucasian population database. Comparisons using artificially degraded samples profiled with both the SNP multiplex and AMPFISTR SGM plus (Applied Biosystems) demonstrated a greater likelihood of obtaining a profile using SNPs for certain sample types. Saliva stains degraded for 147 days generated an 81% complete SNP profile whilst short tandem repeats (STRs) were only 18% complete; similarly blood degraded for 243 days produced full SNP profiles but only 9% with STRs. Reproducibility studies showed concordance between SNP profiles for different sample types, such as blood, saliva, semen and hairs, for the same individual, both within and between different DNA extracts.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Dixon LA,Murray CM,Archer EJ,Dobbins AE,Koumi P,Gill Pdoi
10.1016/j.forsciint.2004.12.011keywords:
subject
Has Abstractpub_date
2005-11-10 00:00:00pages
62-77issue
1eissn
0379-0738issn
1872-6283pii
S0379-0738(04)00817-5journal_volume
154pub_type
杂志文章abstract：:As hatchet blows to the human head frequently cause fatal injuries, the forensic examination of survivors with cranial chop injuries is a rare phenomenon in forensic casework. Besides evaluation of clinical records, photographs, and medico-legal physical examination, the analysis and 3-dimensional reconstruction of pr...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.05.025
更新日期：2016-09-01 00:00:00
abstract：:Fingerprint Recognition System is widely deployed in variety of application domain, ranging from forensic to mobile phones. Its widespread deployment in various applications were person authentication are required, has caused concern that a leaked fingerprint template may be used to reconstruct the original fingerprin...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.04.045
更新日期：2018-07-01 00:00:00
abstract：:The objective of this study was to evaluate the applicability of human identification parameters, established by Tatlisumak et al. (2007), by means of cone-beam computed tomography and extraoral radiographs of the frontal sinus region. From a total of 58 dry skulls, 26 were selected. Posteroanterior, profile cephalome...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.03.030
更新日期：2016-05-01 00:00:00
abstract：:Morphometric cranial variation among Spanish samples was compared to a 19th century Portuguese sample using both traditional and three-dimensional approaches. The Spanish samples included the regional 19th century Oloriz collection and the local 16-17th century Villanubla and Vallolid sample from northwestern Spain. R...
journal_title：Forensic science international
pub_type： 历史文章,杂志文章
doi：10.1016/j.forsciint.2011.01.003
更新日期：2011-03-20 00:00:00
abstract：:A highly polymorphic sequence structure is reported in the human beta-actin related pseudogene 2 (ACTBP2) (SE33) locus in members of the Taiwanese Han population. A total of 100 unrelated members of the Taiwanese Han population were used in the study. Alleles that shared the same size but differ in their sequence are ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(02)00350-x
更新日期：2002-12-04 00:00:00
abstract：:This study investigated the effect of seasonal variables on decomposition in the early post-mortem period using 26 donated human cadavers at the University of Tennessee's Anthropology Research Facility (ARF), USA. The rate and pattern of decomposition in human cadavers (as measured by TBS and the revised TBSsurf metho...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2020.110419
更新日期：2020-10-01 00:00:00
abstract：BACKGROUND:Internationally, urine on-site testing has been used for detecting drivers under the influence of drugs (DUID) but more and more countries, such as Belgium, are switching to oral fluid screening. OBJECTIVE:To compare the previous (published in 1999) and current (published 2009) enforcement procedures of DUI...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.01.034
更新日期：2015-04-01 00:00:00
abstract：:Benzodiazepines are one of the most widely prescribed drugs for the treatment of a wide spectrum of clinical disorders. They are used as anticonvulsants, anxiolytics, hypnotics or muscle relaxants with different duration of action. In this paper, a simple and sensitive method for the determination of benzodiazepines i...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(00)00226-7
更新日期：2000-09-11 00:00:00
abstract：:DNA typing of 15 short tandem repeat (STR) loci included in the AmpFlSTR Identifiler PCR amplification kit (Applied Biosystems), was carried out in three Egyptian populations of different ethnic groups: the Berbers from the Siwa oasis (in the North-Western Egyptian desert), the Muslims and the Copts from Adaima (Upper...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.03.017
更新日期：2007-07-04 00:00:00
abstract：:Fifteen autosomal short tandem repeat (STR) markers (D3S1358, HUMTH01, D21S11, D18S51, PENTA E, D5S818, D13S317, D7S820, D16S539, CSF1PO, PENTA D, HUMvWA, D8S1179, HUMTPOX, FGA) were analyzed in 1734 individuals living in urban areas of cities from six different Argentinian provinces (Buenos Aires, Neuquén, Tucumán, L...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.02.042
更新日期：2007-01-05 00:00:00
abstract：:Pentaerythritol tetranitrate (PETN) and its degradation products are analyzed to discriminate between residues originating from PETN explosions and residues obtained under other circumstances, such as natural degradation on textile, or after handling intact PETN. The degradation products observed in post-explosion sam...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.03.042
更新日期：2013-07-10 00:00:00
abstract：:The formation of adipocere slows further decomposition and preserves corpses for decades or even centuries. This resistance to degradation is a serious problem, especially with regard to the reuse of graves after regular resting times. We present results from an exhumation series in modern graveyards where coffins fro...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.09.010
更新日期：2015-12-01 00:00:00
abstract：:Usually, the differentiation of inks on questioned documents is carried out by optical methods and thin layer chromatography (TLC). Therefore, spectrometric methods were also proposed in forensic literature for the analysis of dyes. Between these techniques, laser desorption/ionization mass spectrometry (LDI-MS) has d...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2010.05.027
更新日期：2011-01-30 00:00:00
abstract：:Identifying the anatomical origin of skeletal elements is a basic and important part of a forensic anthropological investigation, but techniques for determining the side and ray of the phalanges are conspicuously scarce in the physical anthropology literature. Features of particular phalanges are important to aspects ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.09.012
更新日期：2009-12-15 00:00:00
abstract：:During the past decade, several studies have been carried out using the calcaneus bone for sex estimation. This paper collected data using ten variables for metric characteristics of the calcaneus of 144 modern Cretans and examined their correlation with known sex. Secondly, the formulae developed by Peckmann et al. (...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.04.005
更新日期：2017-08-01 00:00:00
abstract：:Suicides with chain saws and circular saws are extreme exceptions, in particular with regard to females and concerning the neck region. We report two of such rare cases involving young women. A 30-year-old female with a known schizoaffective psychosis killed herself with an electric chain saw. The autopsy revealed a d...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.05.019
更新日期：2009-09-10 00:00:00
abstract：:Sex estimation is an integral aspect of biological anthropology. Correctly estimating sex is the first step to many subsequent analyses, such as estimating living stature or age-at-death. Klales et al. (2012) [6] provided a revised version of the Phenice (1969) [3] method that expanded the original three traits (ventr...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.05.001
更新日期：2017-08-01 00:00:00
abstract：:Though the child abuse syndrome is usually considered to have been first described in the middle of the present century, references to child abuse occur throughout history and in the 19th century, detailed descriptions were published in the French medical literature. ...
journal_title：Forensic science international
pub_type： 历史文章,杂志文章
doi：10.1016/0379-0738(86)90008-3
更新日期：1986-02-01 00:00:00
abstract：:The extent of aspartic acid racemization in human dentin can be used to assess chronological age of the individual. This novel chemical method of aging human remains has potential applications in forensic sciences. Six autopsy cases have been analyzed for D/L aspartic acid contents, and their ages at death calculated....
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(86)90080-0
更新日期：1986-11-01 00:00:00
abstract：:The increasing interest in toxicological hair analysis as a marker of human exposure to xenobiotics such as illicit substances or therapeutic drugs, has been made feasible by the extension of mass spectrometry, a highly sensitive method of detection. A newborn exposed to drugs in utero can suffer from a varying degree...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(03)00050-1
更新日期：2003-04-23 00:00:00
abstract：:The hair of 97 cases were analysed for ethyl glucuronide (EtG) and fatty acid ethyl esters (FAEE, including ethyl myristate, ethyl palmitate, ethyl oleate and ethyl stearate) according to the Society of Hair Testing guidelines to examine the role of both tests in documenting chronic excessive alcohol drinking, particu...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.03.012
更新日期：2014-10-01 00:00:00
abstract：:This paper presents a novel digital watermarking technique using face and demographic text data as multiple watermarks for verifying the chain of custody and protecting the integrity of a fingerprint image. The watermarks are embedded in selected texture regions of a fingerprint image using discrete wavelet transform....
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.08.019
更新日期：2007-07-04 00:00:00
abstract：:Buprenorphine is available in Singapore as substitution treatment for opioid dependence since 2002. This study surveys buprenorphine related deaths in Singapore between September 2003 and December 2004. The aims are to establish the autopsy prevalence of buprenorphine related deaths and the demographical and toxicolog...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.03.037
更新日期：2006-10-16 00:00:00
abstract：:The value of complex analyses of body injuries and clothing has been proven by practice. The purpose of presented study is to discuss what additional information can be obtained during investigations of clothes in the area of injury. A case study, in which results of visual and stereomicroscopic morphological analysis...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2004.09.023
更新日期：2004-12-02 00:00:00
abstract：:A new method for diatom detection is described. It includes an ultrasonic irradiation procedure with the use of a tissue solubilizer. The method is easy to carry out and is less time-consuming than previous techniques. ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(80)90181-4
更新日期：1980-07-01 00:00:00
abstract：:In prosecution, Belgian judiciary currently uses outdated yield figures (28.1g per plant, sold at € 3/g at grower level) for fining illicit indoor cannabis plantations. Using state-of-the-art cultivation techniques, our growth experiments showed that yield is better expressed in g/m(2) cultivated surface area rather t...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2012.03.013
更新日期：2012-07-10 00:00:00
abstract：:As well as being the route for gaseous exchange, the nasal passages protect the lungs from debris and fine particulates inhaled during breathing. Foreign particles are trapped by mucus produced by membranes that line the upper respiratory tract. Even when a corpse has skeletonised, particles can be retrieved from the ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.11.019
更新日期：2006-11-22 00:00:00
abstract：:The analysis of condom traces has recently been added to the standard forensic examination protocol of sexual assault and rape cases. Several recent studies have thus focussed on the detection of condom components and classification of the chemical profiles using statistics, obtaining very promising results. The purpo...
journal_title：Forensic science international
pub_type： 杂志文章,评审
doi：10.1016/j.forsciint.2020.110255
更新日期：2020-05-01 00:00:00
abstract：:The experience of the Northern Ireland State Pathology Service is drawn on to illustrate the forensic aspects of death by shooting and bombing caused by terrorists. The investigations into these deaths have to contend with rumours and allegations which can inflame feelings in the community or mislead the investigators...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(88)90215-0
更新日期：1988-01-01 00:00:00
abstract：:Downstairs falls frequently occur within domestic environments and are mainly associated with elderly and intoxicated individuals, often feature multiple injuries on various parts of the body. In most cases it is not possible to determine the cause of the fall and/or death solely by means of external examination. In t...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2003.12.016
更新日期：2004-05-10 00:00:00
