Abstract：
:A duplex primer set for the amplification of mitochondrial DNA HVI and HVII control regions was evaluated for the optimization of a DNA sequencing protocol suitable for forensic casework. HVI and HVII products, with the absence of non-specific products, could be detected by agarose gel electrophoresis when as little as 0.5 and 0.1pg of DNA were amplified for 34 and 38 cycles, respectively. Because HVI and HVII amplicons are co-synthesized in the duplex PCR, fewer steps are required (lessening the risk of cross contamination events) and more frugal use of precious extracted DNA samples is possible, both desirable features for forensic casework. The ABI Prism BigDyetrade mark version 1.1 chemistry provided high quality sequencing data, with little or no background noise and uniform peak heights, outcomes that favored reliable detection of heteroplasmy, particularly at early sequence reads (<40 bases). Optimal compromise between sensitivity and sequence accuracy in the absence of noise was achieved starting at 150 mitochondrial genome copies. The protocol is effective (no sequence errors) with highly degraded DNA (average detectable template size of 200bp). Dual artificial template mixtures with the minor component at 15% suggests that heteroplasmy should be detected at this level with confidence.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Chong MD,Calloway CD,Klein SB,Orrego C,Buoncristiani MRdoi
10.1016/j.forsciint.2004.09.128keywords:
subject
Has Abstractpub_date
2005-11-25 00:00:00pages
137-48issue
2-3eissn
0379-0738issn
1872-6283pii
S0379-0738(04)00665-6journal_volume
154pub_type
杂志文章abstract：:A total of 201 males from Somalia were typed for the Y-chromosome STRs DYS19, DYS385a/b, DYS389-I, DYS389-II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439 with the PowerPlex Y kit (Promega). A total of 96 different haplotypes were observed and the haplotype diversity was 0.9715. The number of unique haplo...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.01.011
更新日期：2005-07-16 00:00:00
abstract：:A fraction of SIDS cases have death delayed by successful CPR, yet they have not been compared to SIDS cases which were found dead or not successfully resuscitated. Our aims were to: (1) determine the percent of SIDS cases in the San Diego SIDS Research Project database for whom death was delayed by CPR and subsequent...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2007.09.009
更新日期：2008-04-07 00:00:00
abstract：:Fatty acid ethyl esters (FAEE) are direct metabolites of ethanol and have been shown to be suitable markers for the evaluation of alcohol consumption. Previous research has suggested that the regular use of alcohol containing cosmetic products can influence the concentration of FAEE detected in hair. In this study we ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.12.002
更新日期：2018-02-01 00:00:00
abstract：INTRODUCTION:During the year 2011 the Egyptian revolution arose with a change in the trend of eye trauma in Egypt. AIM OF WORK:This study aims at reviewing the epidemiology of ocular trauma presenting to Ain Shams University teaching hospital during the year 2011 and comparing it with epidemiology during the previous ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.09.021
更新日期：2013-12-10 00:00:00
abstract：:We have determined the allele and genotype frequencies at the hypervariable locus D1S80 in a native Kuwaiti population using the polymerase chain reaction technique and subsequent high resolution gel electrophoresis. In a sample of 200 individuals, 21 alleles and 57 genotypes were detected. The alleles with 18 and 24 ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(95)01881-6
更新日期：1996-04-02 00:00:00
abstract：:The allelic distribution of 15 short tandem repeat (STR) loci included in the AmpFl STR Identifiler kit was examined in 100 Changdu Tibetan and 118 Naqu Tibetan unrelated individuals living in the Tibet Province, PR China. The distribution of these observed genotypes was not significantly different from the expected d...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.03.012
更新日期：2007-07-04 00:00:00
abstract：:Early in 2009, a state policing agency raided a clandestine drug laboratory in a suburb of a major city in Australia. During the search of the laboratory, a small glass jar labelled "Gamma Source" and containing a green powder was discovered. The powder was radioactive. This paper documents the detailed nuclear forens...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.04.004
更新日期：2014-07-01 00:00:00
abstract：:Blunt (non-penetrating) aortic injuries, in which the arterial wall is damaged in the direction from the intima towards the adventitia, are most commonly the result of a traffic accident. The various forms of blunt aortic injuries, from limited laceration of the intima to complete transection of the aorta, depend on t...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.10.026
更新日期：2010-02-25 00:00:00
abstract：:Teeth are recently drawing attention for their potential as biological modeling investigation samples due to their ability to be collected and their slow substance metabolism. There is no active metabolism of elements after the completion of dentin. Dentin is surrounded by enamel and cementum, and is not affected by t...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2011.11.012
更新日期：2012-06-10 00:00:00
abstract：:The abuse or misuse of forged erectile-dysfunction drugs, containing phosphodiesterase type 5 inhibitors (e.g. sildenafil), is a serious issue globally. Therefore, the detection of sildenafil and related active analogues in counterfeit pharmaceuticals or the differentiation between counterfeit and authentic drugs has ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.08.016
更新日期：2015-12-01 00:00:00
abstract：:DNA molecular techniques were used in a forensic investigation involving the poaching of wildlife in a national park of Italy. A poacher, after having snared a wild boar (Sus scrofa) sow, knifed it to death. The animal was retrieved by conservation officers at the scene before the poacher could remove the carcass. Sub...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.04.032
更新日期：2005-10-29 00:00:00
abstract：:The extent of aspartic acid racemization in human dentin can be used to assess chronological age of the individual. This novel chemical method of aging human remains has potential applications in forensic sciences. Six autopsy cases have been analyzed for D/L aspartic acid contents, and their ages at death calculated....
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(86)90080-0
更新日期：1986-11-01 00:00:00
abstract：:3-Methylmethcathinone (3-MMC or metaphedrone) is a synthetic cathinone, recently introduced in the market of the new psychoactive substances (NPS), initially to replace mephedrone (4-methylmethcathinone or 4-MMC), and rapidly widespread among drug users. 3-Methylmethcathinone is legally controlled in many countries, b...
journal_title：Forensic science international
pub_type： 杂志文章,评审
doi：10.1016/j.forsciint.2018.11.024
更新日期：2019-02-01 00:00:00
abstract：:Tramadol is an opioid drug metabolised in phase I by cytochrome P450 (CYP) enzymes, of which CYP2D6 is mainly responsible for the O-demethylation of tramadol, but is not involved in N-demethylation. Defects in the genes encoding drug metabolising enzymes (DMEs) may lead to adverse drug effects, even to death. To aid i...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(03)00159-2
更新日期：2003-07-29 00:00:00
abstract：:Ten X-chromosomal STRs from two multiplex PCR approaches (DXS9898, DXS6807, HPRTB, DXS101, and androgen receptor (ARA); DXS7133, DXS10011, DXS7424, DXS8377, and DXS8378) have been typed in a Latvian population sample. ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.04.006
更新日期：2006-03-10 00:00:00
abstract：:A 66-year-old man received a local infiltration of cortisone and xylocain in the left paravertebral region between the fifth and sixth cervical vertebrae. Respiratory failure occurred 2.5 h later with subsequent successful resuscitation. During the following 2 months of assisted ventilation up to the patients death, t...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2004.05.018
更新日期：2005-05-10 00:00:00
abstract：:The use of amphetamine and 'ecstasy' (MDMA) has increased exponentially in many European countries since the late nineties, leading to a rapid growth in the number of clinical and forensic analyses. Therefore, a rapid screening procedure for these substances in biological specimens has become an important part of rout...
journal_title：Forensic science international
pub_type： 临床试验,杂志文章
doi：10.1016/j.forsciint.2005.04.019
更新日期：2005-10-04 00:00:00
abstract：:Forensic material of alcoholics was compared with controls with regard to the height, weight, state of nutrition, stature, the ageing process and the presence of traumatic lesions. Surprisingly, it was found that alcoholic women were taller than controls. Alcoholics were significantly more often slightly undernourishe...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(94)01641-h
更新日期：1995-01-21 00:00:00
abstract：:The identification of cadavers (the main activity of forensic odontologists and anthropologists) is a crucial issue in forensic pathology, but the official entity of this problem is still poorly known in most countries, apart from a few American reports. In this article the authors present a descriptive study of unide...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.11.008
更新日期：2010-02-25 00:00:00
abstract：:Successional patterns of insect fauna on pig carcasses were studied in southwest Virginia. The objective was to identify and qualitatively assess the major taxa of forensic importance in this region. Studies were conducted in spring and summer 2001 and 2002, and fall 2002. Over 50 taxa were collected and identified. P...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2004.06.041
更新日期：2005-05-28 00:00:00
abstract：:A very serious case of DFSA (drug facilitated sexual assault) is presented, in which a six-year-old girl died following sedation with γ-hydroxybutyric acid (GHB). She had been sexually abused by a relative. Samples of cardiac blood, bile, vitreous humour, liver, kidney, brain tissues and hair were analysed by a LC-MS/...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.12.044
更新日期：2016-02-01 00:00:00
abstract：:Synthetic cannabinoids are a group of new psychoactive compounds (NPS) that act as agonists at the cannabinoid receptor. First reported in 2008, they currently represent one of the largest groups of NPS that are monitored by the European Monitoring Centre for Drugs and Drug Addiction (EMCDDA). Five samples (4 from the...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.10.020
更新日期：2017-12-01 00:00:00
abstract：:This article describes the first use of an anti-Stokes material, or up-converter, for the development of latent fingermarks on a range of non-porous surfaces. Anti-Stokes materials can absorb long-wavelength light and emit light at a shorter wavelength. This property is unusual in both natural and artificial materials...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2010.09.020
更新日期：2011-04-15 00:00:00
abstract：:Injury scales have two main applications on homicide investigations, namely, to evaluate the severity of the victims' injuries and to identify the profiles of the offenders. However, few studies have examined the quality of the various injury scales in serving the two purposes. In this study, homicides from Shanghai a...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.09.010
更新日期：2018-11-01 00:00:00
abstract：:A new method for diatom detection is described. It includes an ultrasonic irradiation procedure with the use of a tissue solubilizer. The method is easy to carry out and is less time-consuming than previous techniques. ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(80)90181-4
更新日期：1980-07-01 00:00:00
abstract：:This report describes an autopsy case of a rare longitudinal brainstem laceration associated with complex basilar skull fractures. The victim was a 40-year-old male who died immediately after falling from a roof (9.2m in height) of a factory onto a concrete floor. The postmortem examination revealed an incomplete ring...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(02)00029-4
更新日期：2002-03-28 00:00:00
abstract：:This paper describes the first reported death involving ocfentanil, a potent synthetic opioid and structure analogue of fentanyl abused as a new psychoactive substance in the recreational drug scene. A 17-year-old man with a history of illegal substance abuse was found dead in his home after snorting a brown powder pu...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.07.005
更新日期：2016-09-01 00:00:00
abstract：:The present report describes the unexpected death of a 6-month-old female infant who had been clinically diagnosed with Joubert syndrome. This is a relatively rare congenital neurological disorder characterized by hypoplasia/aplasia of cerebellar vermis, which transmits information from the body to the cerebellum, and...
journal_title：Forensic science international
pub_type： 杂志文章,评审
doi：10.1016/j.forsciint.2008.06.003
更新日期：2008-08-06 00:00:00
abstract：:A few cases of the sudden unintended acceleration have been reported over the last few years [1-11] and some of them seemed to be somewhat related to an electronic throttle control (ETC) system [11,12]. In this experimental study, efforts were made to reproduce the cases of sudden unintended acceleration possibly rela...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.08.014
更新日期：2016-10-01 00:00:00
abstract：:This article describes a selective gas chromatographic method for the resolution and quantification of phenoperidine and its two metabolites, pethidine (meperidine) and norpethidine (normeperidine). Drugs and SKF 525 A, the internal standard, are separated from plasma by solvent extraction under alkaline conditions. T...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(89)90154-0
更新日期：1989-12-01 00:00:00
