Abstract：
:The 4977 bp deletion of mitochondrial DNA (mtDNA) accumulates in postmitotic tissues with advancing age. The purpose of our study was to detect and quantify these deletion even in blood cells with a high turnover activity. Whole venous blood, isolated human platelets and peripheral blood mononuclear cells (PBMCs) were collected from 10 unrelated donors aged 20-71 years and total DNA was extracted. PCR was performed for total and mutated mtDNA using two different primer pairs and two fluorogenic probes labeled with the fluorescent dyes FAM and VIC. Specific PCR products were generated, detected and quantified in a real-time PCR. The amplification products of total and deleted mtDNA could be detected in each sample and did not exhibit any differences in the amount of the deleted mtDNA in whole blood, human platelets or PBMCs. Our data did not show any accumulation of the 4977 bp deletion with increasing age as it was observed for several other tissues.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Meissner C,Mohamed SA,Klueter H,Hamann K,von Wurmb N,Oehmichen Mdoi
10.1016/s0379-0738(00)00249-8keywords:
subject
Has Abstractpub_date
2000-09-11 00:00:00pages
109-12issue
1-3eissn
0379-0738issn
1872-6283pii
S0379073800002498journal_volume
113pub_type
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