Abstract：
:Cannabis is the one of the most frequently detected drug in workplace drug testing and in cases of driving under influence of drugs, and there is a greater demand for sensitive, rapid and reliable methods for confirming the presence of this drug in biological samples. This paper describes an LC-MS-MS procedure for direct analysis of cTHC and cTHC-glucuronide in urine, without hydrolysis of the samples or derivatisation. The sample preparation is very simple and consist only in a dilution of urine with methanol 1:1 (v/v) after adding the deuterated internal standard (cTHC-d3); then 10μl of the final solution is injected in LC-MS-MS. Chromatographic separation was performed using a reversed-phase column and gradient elution with 0.01% formic acid/acetonitrile/methanol and two MS-MS transitions for each substance were monitored. The method was fully validated and proved to be accurate (RSD <15%), precise (intra-day CV <10%) and sensitive with a limit of detection (LOD) of 5ng/ml for both the compounds studied. Linearity was tested in the range 5-1000ng/ml and the values of R(2) resulted >0.99. The developed method was applied to several authentic samples of urine which tested positive in the immunoassay screening and 98% of them was confirmed.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Felli M,Martello S,Chiarotti Mdoi
10.1016/j.forsciint.2010.05.004subject
Has Abstractpub_date
2011-01-30 00:00:00pages
67-73issue
1-3eissn
0379-0738issn
1872-6283pii
S0379-0738(10)00253-7journal_volume
204pub_type
杂志文章abstract：:The hairy maggot blow fly, Chrysomya rufifacies (Diptera: Calliphoridae), is a forensically important fly often encountered on human and other vertebrate remains in temperate and tropic regions throughout the world including Australia, Asia, Central America and North America. C. rufifacies was reared under controlled ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.09.023
更新日期：2014-12-01 00:00:00
abstract：:Following the introduction of limit values for blood alcohol and breath alcohol concentrations of 0.5 g/kg and 0.25 mg/L, respectively, as provided under s. 24 a of the German Road Traffic Act the question is whether also breath alcohol concentrations can be back calculated to the time of the traffic offence in cases ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2004.03.023
更新日期：2004-07-16 00:00:00
abstract：:Therapeutic (or non-toxic) postmortem guaifenesin blood and liver concentrations have not been previously described. Peripheral blood guaifenesin concentrations were compared to central blood and liver concentrations in eight medical examiner cases. Specimens were initially screened for alcohol and simple volatiles, d...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.10.029
更新日期：2014-12-01 00:00:00
abstract：:Forensic personnel may face a daunting task when searching for buried weapons at crime scenes or potential disposal sites. In particular, it is common to search for a small firearm that was discarded or buried by a perpetrator. When performing forensic searches, it is recommended to first use non-invasive methods such...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2010.10.019
更新日期：2011-05-20 00:00:00
abstract：:The Mombello Psychiatric Hospital in Limbiate, near Milan, replaced the old Senavra Hospital as the Psychiatric Hospital for the Province of Milan in the 19th century. During the early 20th century, bodies of several Mombello patients were dissected and preserved by Giuseppe Paravicini, an anatomist who operated withi...
journal_title：Forensic science international
pub_type： 历史文章,杂志文章
doi：10.1016/j.forsciint.2016.11.011
更新日期：2017-01-01 00:00:00
abstract：:Fungi colonizing cadavers are capable of drug metabolism and may thus change the metabolite pattern or concentration of drugs in forensic postmortem samples. The purpose of this study was to check for the presence of such changes by searching fungi-specific metabolites of four model drugs (amitriptyline, metoprolol, m...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.03.006
更新日期：2016-05-01 00:00:00
abstract：:Traditionally, sperms are isolated from vaginal cell mixtures by preferential extraction methods. Although these methods work well when there is a reasonable amount of DNA present, they are problematic when there are limited amounts (ca. 250 pg). In particular, the analysis of sperm from microscope slides has proven d...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(03)00267-6
更新日期：2003-10-14 00:00:00
abstract：:To investigate the DNA mutation rate and pattern in the hypervariable short tandem repeat (STR) locus D12S391 and in the locus D8S1132, samples from an eastern German population (Dresden area) were analysed. A duplex PCR was applied, using short amplification products for D12S391 (129-177bp) and a modified reverse pri...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(01)00596-5
更新日期：2001-12-27 00:00:00
abstract：:Recovery, identification, and burial of casualties from World War I and II is still a topic of interest in many countries. In Slovakia, recent discoveries of such remains are usually accidental. Unfortunately, in many cases the remains are disrupted and without any material culture present, rendering the identificatio...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2020.110673
更新日期：2021-02-01 00:00:00
abstract：:In order to evaluate histological features of freezing damages to human tissue after death, we froze samples of liver and heart tissue to temperatures of -12 degrees C, -28 degrees C and -80 degrees C, and stored them for 24 and 72 h, respectively, at those temperatures. After thawing and routine preparation for histo...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(99)00043-2
更新日期：1999-06-28 00:00:00
abstract：:With research providing conflicting results from different habitats across the globe, the effect of clothing on decomposition is unclear; some studies indicate clothing increases decomposition rate by facilitating increased insect activity, and others conclude clothing prolongs the decay process. In South Africa, such...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2020.110197
更新日期：2020-04-01 00:00:00
abstract：:The aim of this study is to investigate the potential use of reconstructed three-dimensional multi-detector computed tomography (3D MDCT) imagery to distinguish between perimortem cranial trauma and postmortem cranial damage. A total of 45 crania were initially examined for the purpose of this study. The postmortem gr...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.03.032
更新日期：2013-06-10 00:00:00
abstract：:This paper presents real world acceleration data for an ambulance driving up and over a curb. A full scale reenactment was performed for a litigated case in which a patient on a gurney in an ambulance claimed a variety of bodily injuries after the ambulance struck a curb. A height and weight matched surrogate rode on ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.01.023
更新日期：2014-04-01 00:00:00
abstract：:One hundred and seventy-six plants of 22 different lots of Cannabis sativa L., grown at the Botanical Garden of Siena (Italy) were chromatographically analysed in order to define the cannabinoid content in their leaves. The content of the major cannabinoids, delta 9-tetrahydrocannabinol, cannabidiol, cannabinol and ca...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(84)90149-x
更新日期：1984-01-01 00:00:00
abstract：:Testing human hair for drugs of abuse is a relatively new technique which requires control before being fully accepted in justice applications. Laboratories must be able to demonstrate that they can accurately determine what drugs are present in unknown hair samples and at what levels. To date few exercises have been ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(96)02058-0
更新日期：1997-01-17 00:00:00
abstract：:Sex identification of forensic samples (bloodstains and decomposed tissue) by polymerase chain reaction (PCR) was investigated. Amplification of a segment of the amelogenin gene using a pair of primers revealed both Y- and X-specific bands at the same time. The gene has counterparts in both the X and Y chromosomes and...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(91)90174-h
更新日期：1991-01-01 00:00:00
abstract：:Forensic samples are commonly influenced by various environmental factors, including ultraviolet (UV) irradiation; thus, forensic applications of DNA repair (e.g., PreCR™, Restorase®) have been investigated, focusing on short tandem repeat typing. However, current DNA-based examinations are used for both human and bod...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.110077
更新日期：2020-01-01 00:00:00
abstract：:Two sewing needles were found in a woman's cranial cavity by X-ray examination after she had sustained traffic injury. After investigation and analysis, it has been confirmed that the sewing needles were inserted by others when she was an infant, and not by the traffic injury. ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(01)00470-4
更新日期：2001-10-15 00:00:00
abstract：:Norway introduced legislative limits for driving under the influence of drugs (DUID) February 1st, 2012, to harmonize with the legislation on driving under the influence of alcohol. Per se limits corresponding to blood alcohol concentrations (BACs) of 0.02% were established for 20 drugs and concentration limits for gr...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.10.038
更新日期：2014-12-01 00:00:00
abstract：:DNA typing of 15 short tandem repeat (STR) loci included in the AmpFlSTR Identifiler PCR amplification kit (Applied Biosystems), was carried out in three Egyptian populations of different ethnic groups: the Berbers from the Siwa oasis (in the North-Western Egyptian desert), the Muslims and the Copts from Adaima (Upper...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.03.017
更新日期：2007-07-04 00:00:00
abstract：:Missing, ineligible or delayed reference data to establish conventional dental or DNA identification are common scenarios in forensic practice. Therefore, it is worthwhile to explore new avenues that facilitate human identification. Due to the recent remarkable evolution in the prosthetic dental restorations based on ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.109926
更新日期：2019-09-01 00:00:00
abstract：:Data of eight Y-chromosomal STRs, the so called "minimal core set", were obtained from 152 unrelated males of the Primorye region of Russia. The allelic frequencies correspond to other European populations. The background is a settlement of males from the European part of Russia, Ukraine and other states which were in...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.05.018
更新日期：2006-05-25 00:00:00
abstract：:Allele frequencies, forensic parameters for the 11 STR loci (TH01, TPOX, CSF1PO, D13S317, D7S820, D16S539, VWA, FESFPS, F13A01, F13B and LPL) were determined in a sample of 140-1262 unrelated adults from the Upper Silesia region (Poland). Comparison of allele frequencies for examined STR loci between the Upper Silesia...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.01.011
更新日期：2007-05-03 00:00:00
abstract：:In this work, we present population genetic data of 10 X-chromosome STRs (DXS8378, DXS9898, DXS8377, HPRTB, GATA172D05, DXS7423, DXS6809, DXS7132, DXS101 and DXS6789) obtained from sample of 145 unrelated female individuals belonging to Valencia (Spain), a region located in the east of the Iberian Peninsula. All the m...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.10.023
更新日期：2007-12-20 00:00:00
abstract：:The tongue was examined for the presence of haemorrhages in 264 medicolegal autopsy cases. The tongue was sectioned transversely and examined macroscopically and microscopically. Haemorrhages were found in marginal and/or central parts of the tongue in 104 cases. Among them, 28 cases showed haemorrhages in central par...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(82)90127-x
更新日期：1982-11-01 00:00:00
abstract：UNLABELLED:INTRODUCTION/CONTEXT: Torture is widely practiced throughout the world and, yet, the ways by which torture is perpetrated, its regional similarities and differences, is not well understood. Our goal for this cases series was to elucidate the methods of torture practiced within different countries to both add...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.03.026
更新日期：2009-08-10 00:00:00
abstract：:In view of a potential future use for dating hemorrhage in forensic medicine the correlation of MR relaxation parameters with time was evaluated in blood samples. A systematic relationship could be valuable for using MRI for estimating the age of hemorrhage and soft tissue hematomas particularly in clinical forensic m...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.02.032
更新日期：2016-05-01 00:00:00
abstract：:To assess the species composition of necrophagous blow flies (Diptera: Calliphoridae) in the area around the federal city of Switzerland, Bern, sampling with baited bottle traps was performed over the course of 34 weeks in 2014. Six locations ranging from urban to forest habitats were sampled weekly or rather biweekly...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.05.022
更新日期：2018-08-01 00:00:00
abstract：:Since the anthrax mail attacks of 2001, law enforcement agencies have processed thousands of suspicious mail incidents globally, many of which are hoax bioterrorism threats. Bio-insecticide preparations containing Bacillus thuringiensis (Bt) spores have been involved in several such threats in Australia, leading to th...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2011.12.003
更新日期：2012-06-10 00:00:00
abstract：:Fluvoxamine is a selective serotonin reuptake inhibitor that has been considered relatively safe in overdose. At therapeutic and supratherapeutic concentrations, fluvoxamine affects cardiac conduction, prolongs QTc interval, causes hypotension, obtundation, and can increase propensity for seizures. A man in his 60s wa...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.02.047
更新日期：2019-07-01 00:00:00
