What happens in freezing bodies? Experimental study of histological tissue change caused by freezing injuries.

Abstract:

:In order to evaluate histological features of freezing damages to human tissue after death, we froze samples of liver and heart tissue to temperatures of -12 degrees C, -28 degrees C and -80 degrees C, and stored them for 24 and 72 h, respectively, at those temperatures. After thawing and routine preparation for histology, the samples were evaluated both by microscope and with an electronic image analyzer. In all cases, we found extended extracellular spaces and shrunken cells resulting from the freeze-thaw cycle. These features were more pronounced in tissues stored for longer durations. Such findings seem to be typical of tissue that has been frozen prior to examination. Two cases of dead bodies found outdoors at subzero temperatures demonstrate that formerly frozen and unfrozen tissues can be distinguished histologically. The findings are examined in relation to the fundamental laws of cryobiology.

journal_name

Forensic Sci Int

authors

Schäfer AT,Kaufmann JD

doi

10.1016/s0379-0738(99)00043-2

keywords:

subject

Has Abstract

pub_date

1999-06-28 00:00:00

pages

149-58

issue

2-3

eissn

0379-0738

issn

1872-6283

pii

S0379-0738(99)00043-2

journal_volume

102

pub_type

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