Abstract:
OBJECTIVE:Quinoa (Chenopodium quinoa Willd.) is a seed crop rich in bioactive compounds including phytoecdysones (especially 20-hydroxyecdysone, 20HE), polyphenols, proteins and essential fatty acids. We previously reported a method to leach and concentrate quinoa bioactives into a complex phytochemical mixture termed quinoa leachate (QL). Here, we aimed to determine the effect of QL and its chemically distinct fractions on five biochemical endpoints relevant to skin care applications: (i) cell viability, (ii) matrix metalloproteinase (MMP) mRNA expression, (iii) MMP enzymatic activity, (iv) tyrosinase enzymatic activity and (v) intracellular reactive oxygen species (ROS) production. METHODS:Quinoa leachate was fractionated and chemically characterized using column chromatography and liquid chromatography-mass spectrometry (LC-MS). Cell viability was determined using a MTT assay in four mammalian cell lines. MMP-1 mRNA expression was assessed in human dermal fibroblasts (HDF) via qRT-PCR. The enzymatic activity of MMP-9 and tyrosinase was measured using fluorometric and colorimetric in vitro assays, respectively. Lipopolysaccharide (LPS)-induced ROS production was determined in human dermal fibroblasts by fluorescence intensity of an oxidant-sensitive probe. RESULTS:Quinoa leachate was separated into three fractions: (i) carbohydrate-rich fraction (QL-C; 71.3% w/w of QL); (ii) phytoecdysone, polyphenol and protein-rich fraction (QL-P, 13.3% w/w of QL); (iii) oil-rich fraction (QL-O, 10.8% w/w of QL). QL did not reduce cell viability in any of the four cell lines tested. QL, QL-P and QL-O each significantly inhibited MMP-1 mRNA expression in HDF at a concentration of 5 μg mL(-1) . QL and QL-P also significantly inhibited MMP-9 enzymatic activity, whereas QL-P demonstrated significant tyrosinase enzymatic inhibition. Furthermore, QL, QL-P, QL-O and 20HE significantly inhibited intracellular ROS production. CONCLUSION:This study is the first to demonstrate the MMP, tyrosinase and ROS inhibiting properties of multiple different phytochemical components derived from quinoa seeds. Our work indicates that quinoa phytochemicals may play a role in the treatment and prevention of skin ageing through a multiplicity of effects.
journal_name
Int J Cosmet Scijournal_title
International journal of cosmetic scienceauthors
Graf BL,Cheng DM,Esposito D,Shertel T,Poulev A,Plundrich N,Itenberg D,Dayan N,Lila MA,Raskin Idoi
10.1111/ics.12185subject
Has Abstractpub_date
2015-04-01 00:00:00pages
212-21issue
2eissn
0142-5463issn
1468-2494journal_volume
37pub_type
杂志文章abstract::Synopsis The absorption of ultraviolet light by a film of sunscreen product on the surface of the skin is considered from a theoretical standpoint. It is apparent that Beer-Lambert's Law would predict protection factors in excess of the actual factors by many orders of magnitude. The possible reasons for this are disc...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.1985.tb00417.x
更新日期:1985-10-01 00:00:00
abstract::Sweat and skin surface pH are critical parameters in determining the performance of antiperspirants. The mechanism of action, the so-called 'plug theory' first proposed by Reller and Luedders, involves the expression of eccrine sweat onto the surface of the skin into which the solid antiperspirant salts, typically an ...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1046/j.1467-2494.2001.00092.x
更新日期:2001-08-01 00:00:00
abstract::There is an increasing demand in general population regarding skin healthiness and improvement of aesthetical appearance, indicating that people require more information about how to treat healthy skin and to prevent skin disease. This study is the result of a campaign on healthy skin organized by the International So...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.2005.00287.x
更新日期:2005-12-01 00:00:00
abstract::On a daily basis, the skin is exposed to many environmental stressors and insults. Over a 24-h natural cycle, during the day, the skin is focused on protection; while at night, the skin is focused on repairing damage that occurred during daytime and getting ready for the next morning. Circadian rhythm provides the pre...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12572
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVE:To understand the drivers of body skin discomfort and to validate a new index to assess its severity. This index should be sensitive enough to capture changes in response to treatment. METHODS:Previous consumer studies suggested seven potential main dimensions behind skin discomfort. Four of them refer to se...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12490
更新日期:2018-10-01 00:00:00
abstract::The vapour pressures of phenethyl alcohol and limonene in their water-in-oil microemulsion region were measured by head space gas chromatography of equilibrated samples. The results showed that the vapour pressure of both fragrance compounds in their mutual solutions without surfactant or water was significantly great...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1046/j.1467-2494.1998.177054.x
更新日期:1998-12-01 00:00:00
abstract:OBJECTIVE:A novel microwave-assisted dispersive liquid-liquid microextraction (MADLLME) technique according to the solidification of a floating organic droplet (SFO) and graphite furnace atomic absorption spectrometry (GFAAS) used for the extraction and determination of lead ions in lipsticks and hair dyes made in diff...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12221
更新日期:2015-10-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to perform short-term sensory testing and instrumental (conductivity and rheological) characterization of a fast inverted oil-in-water (o/w) emulsion base, also known as a SWOP (Switch-Oil-Phase) emulsion, and reference o/w and water-in-oil (w/o) emulsion bases under various testing ...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12282
更新日期:2016-06-01 00:00:00
abstract::The stability constants for the inclusion of fragrance materials with 2-hydroxypropyl-beta-cyclodextrin (2HP-beta-C yD) in aqueous solution have been determined by the static head-space method. The 1:1 stability constants obtained by this method were in reasonable agreement with the corresponding values in the literat...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1046/j.1467-2494.1999.203156.x
更新日期:1999-06-01 00:00:00
abstract:OBJECTIVE:Several studies have shown that a deterioration of skin properties, an impaired cutaneous microcirculatory function and an imbalance of autonomic nervous activity are observed in smokers and in patients with diabetes mellitus or Raynaud's phenomenon. These observations suggest that skin properties are associa...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12373
更新日期:2017-06-01 00:00:00
abstract:OBJECTIVE:To study the effects of addition of a redox metal, copper, antioxidants and metal chelators on the formation of free radicals in natural white Caucasian hair subsequently exposed to UV light. Three different methods, electron paramagnetic resonance (EPR), a fluorescent probe for hydroxyl radical formation (te...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12601
更新日期:2020-04-01 00:00:00
abstract::Body odours are generated from dead skin cells and secreted materials, such as sweat and sebum, through the metabolism of microorganisms living on the skin. Volatile steroids, key compounds in body odours, are also generated through the metabolism of microorganisms. These volatile steroids strengthen the intensity of ...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.2006.00297.x
更新日期:2006-02-01 00:00:00
abstract::Skin moisturization is not only important for maintaining skin functional properties but also has great impact on the skin's aesthetic properties. The top layer of the skin, the stratum corneum (SC), plays a key role in protecting and preventing against external aggressions as well as in regulating water flux in and o...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1468-2494.2008.00428.x
更新日期:2008-02-01 00:00:00
abstract::The aim of this work was to investigate the effects of 1,18-octadecen-9-dioic acid (dioic acid) and a Rumex occidentalis extract complex for their skin-lightening action in an Indian population. Prior to the clinical study, the efficacy of dioic as an inhibitor of melanogenesis was confirmed on dark-pigmented human me...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12002
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVES:Hyaluronan (HA), an important constituent of extracellular matrix in the skin, has many biological activities such as hydration that contributes to firmness and bounciness of the skin. We have reported that reduction in HA in the papillary dermis and over-expression of HYBID (HYaluronan Binding protein Invol...
journal_title:International journal of cosmetic science
pub_type: 杂志文章,随机对照试验
doi:10.1111/ics.12505
更新日期:2019-02-01 00:00:00
abstract::Summary The authors studied the combination of Kathon(R) CG and Germall(R) II (0.1%-0.1%) in hydrophilic creams. Their objective was to find an alternative to the use of parabens. Kathon(R) CG was chosen due to its antimicrobial effectiveness and its broad spectrum of activity. It must be indicated, however, that it h...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.1987.tb00463.x
更新日期:1987-04-01 00:00:00
abstract::Acne vulgaris suppresses an individual's self-confidence by causing distress with regard to physical appearance, which affects a significant number of individuals during puberty and is delineated by adolescence. Several treatments have been introduced to decrease the aesthetic and psychological problems caused by acne...
journal_title:International journal of cosmetic science
pub_type: 杂志文章,评审
doi:10.1111/j.1468-2494.2011.00647.x
更新日期:2011-08-01 00:00:00
abstract:OBJECTIVE:Equol has been shown to improve skin health and regeneration, due to its antioxidative, phytoestrogenic and epigenetic characteristics. The effects of a topical intervention on skin structure, telomere length and epigenetic markers in skin cells were analysed. METHODS:Sixty-four participants were divided in ...
journal_title:International journal of cosmetic science
pub_type: 杂志文章,随机对照试验
doi:10.1111/ics.12408
更新日期:2017-10-01 00:00:00
abstract::The stratum corneum is the outermost layer of the skin. It constitutes a permeability barrier that keeps micro-organisms and toxic agents from entering the skin, while retaining water and electrolytes. In order to preserve its colour and elasticity, the skin needs lipids and water. Certain occlusive products in contac...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.2007.00357.x
更新日期:2007-04-01 00:00:00
abstract::Synopsis Oil/water (o/w) microemulsions containing propranolol were studied. Isopropylmyristate was used as the oil, Tween 60 as a surfactant, butanol as a co-surfactant and a buffer of pH 6.5 for the continuous phase. The lipophilicity of propranolol was enhanced by formation of lipophilic ion-pairs to obtain a dispe...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.1988.tb00026.x
更新日期:1988-12-01 00:00:00
abstract::Lecithin has an antioxidative potential that depends directly on the content of phospholipids. Purified lecithin with a higher phospholipid content exerts better antioxidative properties. Furthermore, due to its specific physico-chemical properties, amphiphility in particular, the phospholipid the largest portion of t...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1046/j.1467-2494.1999.181682.x
更新日期:1999-08-01 00:00:00
abstract::The assessment of many aspects of the structure and biological dynamics of the stratum corneum can be conducted using calibrated stripping with adhesive-coated discs (SACD). Squamometry entails staining SACD samples with a toluidine blue-basic fuschsin ethanol-based solution and reading the colorimetric variable Chrom...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Several treatments for skin whitening are available today, but few of them are completely adequate, especially owing to the carcinogenic potential attributed to classical drugs like hydroquinone, arbutin and kojic acid. To provide an alternative and safer technology for whitening, we developed two botanical compounds ...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1468-2494.2012.00736.x
更新日期:2012-10-01 00:00:00
abstract::The phase diagram water, phenethyl alcohol and laureth 4 was determined and the variation of the vapour pressure of the alcohol was determined during evaporation using gas chromatographic head-space analysis. The phase changes during evaporation were estimated from the phase diagram and compared to the appearance of t...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1046/j.1467-2494.1997.171722.x
更新日期:1997-12-01 00:00:00
abstract::The comparison of the results of microscopic, conductimetric and rheologic analyses carried out on the same triple W/O/W emulsion, immediately following its manufacture and 10 years later, indicates the remarkable stability of such system. This stability illustrates the great potential of this formula in the protectio...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1046/j.1467-2494.2000.00020.x
更新日期:2000-04-01 00:00:00
abstract::The color and odor of cosmetics have been shown to be crucial for affective states and able to influence autonomic responses. We report an original procedure to measure the effect on subjects of the color and odor of cosmetic products, and to quantify the correlation between objective (psychophysiological recording) a...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.2003.00196.x
更新日期:2003-12-01 00:00:00
abstract::The photostabilities of four sunscreen products have been assessed in vitro by applying sunscreen to a substrate and measuring the spectral transmission prior to, and after exposure to a source of ultraviolet (UV) radiation. Results were independent of whether an application thickness of 1 or 2 mg/cm ; 2 was used, and...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1046/j.1467-2494.1999.203163.x
更新日期:1999-10-01 00:00:00
abstract:OBJECTIVE:We investigated the association of psychosocial problems, menopausal symptoms such as hot flushes, and trends of smoking and use of cosmetics in our previous study. In this follow-up study, we researched psychiatric disorders and psychosocial problems in menopausal women. METHODS:We designed a cohort study w...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12081
更新日期:2013-12-01 00:00:00
abstract:OBJECTIVE:The roots of the herb Paeonia lactiflora ('White Peony') are used in association with other herbs in traditional clinical cosmetic practice in China as oral treatment for skin pigmentary disorders, such as brown or dark pigmentary spots. However, the skin-depigmenting potential of Paeonia lactiflora root extr...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12309
更新日期:2016-10-01 00:00:00
abstract::The present investigation describes a comparative study for the design of innovative topical formulation for skin hydration. In particular, different colloidal forms based on lipidic components have been produced and characterized. Morphology and dimensional distribution have been investigated by means of electron mic...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.2007.00362.x
更新日期:2007-02-01 00:00:00