Abstract：
:ELISpot is one of the most commonly used immune monitoring assays, which allows the functional assessment of the immune system at the single cell level. With its outstanding sensitivity and ease of performance, the assay has recently advanced from the mere single function cell analysis to multifunctional analysis by implementing detection reagents that are labeled with fluorophores (FluoroSpot), allowing the detection of secretion patterns of two or more analytes in a single well. However, the automated evaluation of such assays presents various challenges for image analysis. Here we dissect the technical and methodological requirements for a reliable analysis of FluoroSpot assays, introduce important quality control measures and provide advice for proper interpretation of results obtained by automated imaging systems.
journal_name
Cellsjournal_title
Cellsauthors
Janetzki S,Rueger M,Dillenbeck Tdoi
10.3390/cells3041102subject
Has Abstractpub_date
2014-11-27 00:00:00pages
1102-15issue
4issn
2073-4409pii
cells3041102journal_volume
3pub_type
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