Abstract:
:Receptors for complexes between alpha 2-macroglobulin (alpha 2M) and proteinases (e.g. trypsin) were identified and characterized in the human placenta. The receptors also bound the complex formed between pregnancy zone protein (PZP) and chymotrypsin, although with slightly lower affinity, whereas binding of alpha 2M or PZP in their native forms was negligible. Treatment with methylamine to cleave the internal thiol esters caused an increase in binding affinity of alpha 2M to the level of alpha 2M-trypsin but only a minor increase in the affinity of PZP. Chorionic villi prepared from normal full-term placentae were approximately half occupied by endogenous alpha 2M or PZP complexes. These ligands, as well as prebound 125I-labelled alpha 2M-trypsin, were rapidly removed by the addition of 5 mM EDTA. Binding was similar in villi from eight-week and full-term placentae. Autoradiography showed that labelled alpha 2M-trypsin was associated with the syncytiotrophoblast. The kinetics of 125I-labelled alpha 2M-trypsin binding at 4 degrees C was similar in isolated villi and microvillous membranes. Association was slow, with apparent equilibrium by about 16 h. Dissociation of prebound tracer was slow but markedly accelerated in the presence of unlabelled ligand at a saturating concentration. The concentration-dependence of binding at equilibrium yielded a non-linear Scatchard plot. Most of the binding of ligand at tracer concentration was accounted for by high-affinity receptors with a dissociation constant (Kd) of about 50 pM. The content of high-affinity receptors in one placenta was estimated as approximately 125 pmol, i.e., a significant fraction of the total receptor population in the pregnant woman.
journal_name
Placentajournal_title
Placentaauthors
Jensen PH,Moestrup SK,Sottrup-Jensen L,Petersen CM,Gliemann Jdoi
10.1016/0143-4004(88)90019-7subject
Has Abstractpub_date
1988-09-01 00:00:00pages
463-77issue
5eissn
0143-4004issn
1532-3102pii
0143-4004(88)90019-7journal_volume
9pub_type
杂志文章相关文献
PLACENTA文献大全abstract::Prior studies have shown that levels of corticotrophin-releasing hormone (CRH) in the umbilical cord blood of infants born after pregnancies complicated by pre-eclampsia are significantly higher than fetal plasma CRH concentrations in uncomplicated pregnancies. In the present study we have measured CRH by radioimmunoa...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/0143-4004(95)90095-0
更新日期:1995-06-01 00:00:00
abstract::Human leukocyte antigen (HLA)-G is a non-classical HLA-class I antigen which is predominantly expressed on invasive trophoblastic cells and is postulated to be a mediator of maternal-fetal tolerance. HLA-G interacts with NK cells, can present nonamer peptides and binds CD8 in an analogous manner to classical HLA-I. Th...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1053/plac.1999.0515
更新日期:2000-03-01 00:00:00
abstract::Mice were inoculated with Theiler's murine encephalomyelitis virus (TMEV) on gestational days 1-3 (pre-implantation) or days 4-5 (peri- or post-implantation) or with control cell lysate (days 1-5). Dams were subsequently sacrificed between days 11-14 of gestation, and placentae and fetuses were harvested. Few placenta...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1053/plac.1999.0488
更新日期:2000-05-01 00:00:00
abstract::Placental stem cells are of growing interest for a variety of clinical applications due to their multipotency and ready availability from otherwise frequently discarded biomaterial. Stem cells derived from the placenta have been investigated in a number of disease processes, including wound healing, ischemic heart dis...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/j.placenta.2017.05.010
更新日期:2017-11-01 00:00:00
abstract::Preeclampsia is a leading cause of maternal and fetal morbidity and mortality in high and low-income countries. The aetiology of preeclampsia is multifactorial and remains obscure. Some evidences suggest that altered placental fatty acid oxidation might play a role in the pathogenesis of preeclampsia. To reveal if pla...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2011.11.027
更新日期:2012-02-01 00:00:00
abstract:INTRODUCTION:Preeclampsia is one of the main causes of morbidity and mortality in pregnant women and mothers. Numerous studies showed that microRNAs (miRNAs) played important roles in the occurrence and development of preeclampsia. However, the regulation of microRNA-142-3p (miR-142-3p) in preeclampsia has not been cla...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2021.01.002
更新日期:2021-01-06 00:00:00
abstract::Pregnancy-associated plasma protein-A was assayed in the blood of 347 women during pregnancy, using a new primary standard of PAPP-A as reference. The protein was assayed by antibody-antigen crossed electrophoresis with the lower limit of confident assay being 9.5 micrograms PAPP-A/ml (13 pmol/ml). PAPP-A was first de...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(82)80020-9
更新日期:1982-01-01 00:00:00
abstract::Confocal and conventional indirect immunofluorescence and immunogold electron microscopic methods were applied to examine the distribution of extracellular matrix constituents (collagens types III and IV) in the villi of immature and term human placentae. The immunofluorescence study revealed that collagen type III is...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/0143-4004(91)90493-y
更新日期:1991-11-01 00:00:00
abstract::Effects of placental growth factor (PGF), an angiokine product of fetal trophoblasts and maternal decidual cells, on early decidual angiogenesis are undefined. We used whole-mount immunofluorescence analyses to compare uterus and gestation day 4.5-9.5 mouse implantation sites that differed genetically in fetal or mate...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2014.07.006
更新日期:2014-09-01 00:00:00
abstract:INTRODUCTION:The state of activation of dendritic cells (DCs) at the feto-maternal interface critically contributes to optimal decidual immune responses needed to support fetal-placental development. We recently demonstrated that during healthy pregnancy also peripheral blood DCs (PBDCs), which are easily accessible, a...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2012.10.016
更新日期:2013-01-01 00:00:00
abstract:INTRODUCTION:Chronic abruption oligohydramnios sequence (CAOS) is histologically characterized by diffuse chorioamniotic hemosiderosis (DCH). However, the criteria for the histological evaluation of the extent of CAOS-related hemosiderin deposition (HD) of the membranes and the difference in HD between the chorionic pl...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2021.01.013
更新日期:2021-01-20 00:00:00
abstract:BACKGROUND:Pre-gravid obesity is associated with increased morbidity and mortality for both mother and offspring. Recent studies have demonstrated a heightened inflammatory response both systemically and locally within the adipose and placental tissue in women with pre-gravid obesity, which may play a role in mediating...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2010.12.023
更新日期:2011-03-01 00:00:00
abstract::The recent discovery of a local renin-angiotensin system in trophoblastic tissues has raised many questions regarding its role in the physiology of normal gestation and its implications in the pathophysiology of hypertension during pregnancy. In this article, the authors first review the most interesting aspects of th...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/s0143-4004(96)80003-8
更新日期:1996-03-01 00:00:00
abstract:INTRODUCTION:Chronic villitis of unknown etiology (CVUE) and massive chronic intervillositis (MCI) are placental lesions associated with infiltration of mononuclear cells in the chorionic villi and the intervillous spaces, respectively. It is not well known whether immune cells in CVUE and MCI have similar phenotypic c...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2015.03.008
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVE:Pregestational maternal obesity (PGMO) associates with foetoplacental vascular endothelial dysfunction and higher risk for insulin resistance in the neonate. We characterised the PGMO consequences on the insulin response of the human foetoplacental vasculature. METHODS:Umbilical veins were from pregnancies w...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2019.07.007
更新日期:2019-10-01 00:00:00
abstract::Accumulation of advanced oxidation protein products (AOPPs) is prevalent in obesity, advanced maternal age, diabetes mellitus, and polycystic ovary syndrome. Alterations in the regulation and signaling of angiogenic pathways have been recognized as a link between these conditions and pre-eclampsia. To investigate the ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2013.06.308
更新日期:2013-10-01 00:00:00
abstract::Glucose residues were revealed by light microscopic and fine structural analysis using hexokinase-gold conjugate. In human term placenta specific staining was detected over the stroma of placental villi. Colloidal gold particles were found over the collagen fibrils and reticular lamina of basal membrane. Syncytiotroph...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(05)80203-6
更新日期:1993-09-01 00:00:00
abstract::Mitochondrial function is required to support energetically-demanding processes in the placenta. As such, a compromise in mitochondrial function could severely impact fetal growth and development. Respirometry is a highly useful method for studying mitochondrial function, but is not possible in freeze-thawed mitochond...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2011.11.019
更新日期:2012-02-01 00:00:00
abstract::Throughout its entire lifespan, the placenta is able to produce as well as respond to a variety of inflammatory stimuli. Many signaling molecules and concurrent pathways responsible for the propagation of an inflammatory response have been identified in placental cells. From early developmental stages onward, the secr...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/j.placenta.2005.08.009
更新日期:2006-08-01 00:00:00
abstract:INTRODUCTION:Research suggests that autism spectrum disorder (ASD) has its origins in utero. This study examines the association between evidence of placental histopathology and ASD. METHODS:Administrative claims data and medical records data were used to identify ASD cases (N = 55) and matched controls (N = 199) born...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2017.07.006
更新日期:2017-09-01 00:00:00
abstract:INTRODUCTION:Feeding pregnant broodmares with cereal concentrates has been shown to increase maternal insulin resistance and affect foal metabolism in the short and long-term. These effects are likely to be mediated by the placenta. Here, we investigated feto-placental biometry and placental structure and function at t...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2018.02.003
更新日期:2018-04-01 00:00:00
abstract:INTRODUCTION:Type 1 diabetes (T1D) is associated with adverse pregnancy outcome, usually attributed to hyperglycemia. Recently, we showed that pregnancy outcome in normoglycemic T1D rats was characterized by decreased fetal and placental weight, suggesting impaired placental development. In the present study, we tested...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2014.12.004
更新日期:2015-02-01 00:00:00
abstract:INTRODUCTION:Soluble fms-like tyrosine kinase 1 (sFLT-1) is an anti-angiogenic factor implicated in the pathogenesis of preterm preeclampsia. We evaluated sFLT-1 expression and placental pathology in pregnancies complicated by small for gestational age (SGA) infants (<10th percentile), without evidence of preeclampsia....
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2017.02.011
更新日期:2017-04-01 00:00:00
abstract:INTRODUCTION:The interaction between trophoblast cells and maternal uterine endothelium is important for placental vascular modelling. Nitric oxide (NO) is a potent vasorelaxant that regulates systemic blood pressure and is reduced in preeclampsia. NO may affect placental cell interaction. OBJECTIVES:This study was to...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2014.03.009
更新日期:2014-06-01 00:00:00
abstract::The interaction of cocaine and its analog 2 beta-carbomethoxy-3 beta-(4-iodophenyl) tropane (RTI-55) with the human placental serotonin transporter was investigated. The function of the placental serotonin transporter was assayed using three different approaches: serotonin uptake in purified human placental brush bord...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/0143-4004(94)90018-3
更新日期:1994-04-01 00:00:00
abstract:OBJECTIVE:Bioinformatics analysis indicated that the arrestin ARRDC3 was upregulated in placental tissue from patients with preeclampsia (PE). The study aimed to confirm the finding by examining placenta samples from women with and without early-onset PE and to investigate ARRDC3 roles in trophoblast function. METHODS...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2019.10.009
更新日期:2020-01-01 00:00:00
abstract::We tested the hypothesis that the expression of placental connective tissue growth factor (CTGF) is enhanced in pregnancies complicated by severe preeclampsia (PE) or fetal growth restriction (FGR). CTGF expression was analyzed using immunostaining, western blot and real-time quantitative PCR in placental samples obta...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2009.08.006
更新日期:2009-11-01 00:00:00
abstract::Pregnancy-associated placental protein-A (PAPP-A), first isolated from maternal serum, has been identified as a metalloprotease cleaving insulin-like growth factor binding protein-4 (IGFBP-4). The source of PAPP-A during pregnancy is unclear. We therefore investigated PAPP-A expression during in vitro human villous cy...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1053/plac.2002.0944
更新日期:2003-05-01 00:00:00
abstract::It has been suggested that adenosine is involved in the acute effects of ethanol in a number of tissues. The present study was undertaken to evaluate the role of adenosine on the vascular responses of perfused isolated human placental cotyledons after the acute administration of ethanol. The possibility that ethanol m...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(97)80038-0
更新日期:1997-07-01 00:00:00
abstract::Annexins are soluble proteins that bind to biological membranes containing negatively charged phospholipids, principally phosphatidylserine, in a Ca(2+)-dependent manner. Annexin-A5 (AnxA5), the smallest member of the annexin family, presents unique properties of membrane binding and self-assembly into ordered two-dim...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/j.placenta.2015.01.193
更新日期:2015-04-01 00:00:00