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Confocal and conventional immunofluorescent and immunogold electron microscopic localization of collagen types III and IV in human placenta.

Abstract：

:Confocal and conventional indirect immunofluorescence and immunogold electron microscopic methods were applied to examine the distribution of extracellular matrix constituents (collagens types III and IV) in the villi of immature and term human placentae. The immunofluorescence study revealed that collagen type III is more distinct in the villous stroma of term placenta as compared with that of the first trimester. Collagen type IV was detected mainly in endothelial and epithelial basement membranes and interestingly also to a certain extent in the stroma. Results obtained using immunoelectron microscopy support the proposal that collagen types III and IV are characteristic of stromal and basement membranes, respectively. Stromal collagen type IV is apparently localized in association with the interstitial types of collagen (I and III), in the villous stroma of term placenta.

journal_name

Placenta

journal_title

Placenta

authors

Nanaev AK,Rukosuev VS,Shirinsky VP,Milovanov AP,Domogatsky SP,Duance VC,Bradbury FM,Yarrow P,Gardiner L,d'Lacey C

doi

10.1016/0143-4004(91)90493-y

subject

Has Abstract,Author List Incomplete

pub_date

1991-11-01 00:00:00

pages

573-95

issue

6

eissn

0143-4004

issn

1532-3102

pii

0143-4004(91)90493-Y

journal_volume

12

pub_type

杂志文章

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