Abstract：
INTRODUCTION:The breast cancer resistance protein (BCRP/ABCG2) is an efflux transporter in the placental barrier. By transporting chemicals from the fetal to the maternal circulation, BCRP limits fetal exposure to a range of drugs, toxicants, and endobiotics such as bile acids and hormones. The purpose of the present studies was to 1) determine whether BCRP localizes to highly-ordered, cholesterol-rich lipid raft microdomains in placenta microvillous membranes, and 2) determine the impact of cholesterol on BCRP-mediated placental transport in vitro. METHODS:BCRP expression was analyzed in lipid rafts isolated from placentas from healthy, term pregnancies and BeWo trophoblasts by density gradient ultracentrifugation. BeWo cells were also tested for their ability to efflux BCRP substrates after treatment with the cholesterol sequestrant methyl-β-cyclodextrin (MβCD, 5 mM, 1 h) or the cholesterol synthesis inhibitor pravastatin (200 μM, 48 h). RESULTS AND DISCUSSION:BCRP was found to co-localize with lipid raft proteins in detergent-resistant, lipid raft-containing fractions from placental microvillous membranes and BeWo cells. Treatment of BeWo cells with MβCD redistributed BCRP protein into higher density non-lipid raft fractions. Repletion of the cells with cholesterol restored BCRP localization to lipid raft-containing fractions. Treatment of BeWo cells with MβCD or pravastatin increased cellular retention of two BCRP substrates, the fluorescent dye Hoechst 33342 and the mycotoxin zearalenone. Repletion with cholesterol restored BCRP transporter activity. Taken together, these data demonstrate that cholesterol may play a critical role in the post-translational regulation of BCRP in placental lipid rafts.
journal_name
Placentajournal_title
Placentaauthors
Szilagyi JT,Vetrano AM,Laskin JD,Aleksunes LMdoi
10.1016/j.placenta.2017.04.006subject
Has Abstractpub_date
2017-07-01 00:00:00pages
29-36eissn
0143-4004issn
1532-3102pii
S0143-4004(17)30228-Xjournal_volume
55pub_type
杂志文章相关文献
PLACENTA文献大全abstract：:The transport of large amounts of Ca2+ by the plasma membranes of human placental syncytiotrophoblast is essential to the mineralization of the growing fetal skeleton. We have investigated transport by the basal (fetal-facing) plasma membrane (BPM). Ca2+ was taken up by purified BPM vesicles in a time-dependent manner...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/s0143-4004(05)80451-5
更新日期：1994-02-01 00:00:00
abstract：:Antigenic material previously detected in human placental trophoblastic cells by immunoperoxidase staining using a goat antiserum against the feline RD114 retrovirus structural protein p30 was isolated by immunochromatography from normal syncytiotrophoblast. The antigen was used to immunize mice, and of the monoclonal...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/s0143-4004(84)80001-6
更新日期：1984-11-01 00:00:00
abstract：:Peroxidase (Donor: H(2)O(2)oxidoreductase EC 1.11.1.7) from human term placentae of non-smokers was purified to homogeneity by a combination of NH(4)Cl extraction, affinity chromatography, (NH(4))(2)SO(4)precipitation, ion-exchange and gel filtration chromatography. The homogeneity of purified human placental peroxida...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1053/plac.2000.0537
更新日期：2000-07-01 00:00:00
abstract：BACKGROUND:Dietrich Starck (1908-2001) was a German embryologist who wrote extensive reviews on comparative placentation. FINDINGS:Starck's embryology textbook and his comprehensive review of comparative embryology and placentation give excellent insights into the foundational literature and are extensively referenced...
journal_title：Placenta
pub_type： 传,历史文章,杂志文章
doi：10.1016/j.placenta.2017.08.073
更新日期：2017-10-01 00:00:00
abstract：:The hormone leptin (OB) and its receptor (OB-R) are key homeostatic regulators of mammalian body weight. Two predominant isoforms of OB-R are expressed by alternative splicing: the long form, OB-RL, with full signalling capacity is highly expressed in the hypothalamus and the short, signalling-defective form, OB-Rs, i...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1053/plac.2002.0836
更新日期：2002-07-01 00:00:00
abstract：:Pregnancy-associated plasma protein-A was assayed in the blood of 347 women during pregnancy, using a new primary standard of PAPP-A as reference. The protein was assayed by antibody-antigen crossed electrophoresis with the lower limit of confident assay being 9.5 micrograms PAPP-A/ml (13 pmol/ml). PAPP-A was first de...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/s0143-4004(82)80020-9
更新日期：1982-01-01 00:00:00
abstract：:Transfection using cationic lipid reagents such as DharmaFECT2 is an efficient tool for introducing siRNA into primary human cytotrophoblast cells. However, we now report a limitation to the use of this method as the concentration of DharamFECT2 needed for effective siRNA delivery causes ligand-independent activation ...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2010.11.013
更新日期：2011-02-01 00:00:00
abstract：:A protein immunologically identical to the major basic protein (MBP) of eosinophil granules is present in the plasma of pregnant women and has been localized to human placental X cells using immunofluorescence. This study demonstrates the presence of similar immunoreactive molecules in the placental X cells from sever...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/0143-4004(87)90052-x
更新日期：1987-05-01 00:00:00
abstract：:Preeclampsia is a leading cause of maternal and fetal morbidity and mortality in high and low-income countries. The aetiology of preeclampsia is multifactorial and remains obscure. Some evidences suggest that altered placental fatty acid oxidation might play a role in the pathogenesis of preeclampsia. To reveal if pla...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2011.11.027
更新日期：2012-02-01 00:00:00
abstract：:We developed a model of maternal hyperglycaemia with secondary hyperinsulinaemia and hypoaminoacidaemia in pregnant sheep (H) to determine the effect of these conditions on uterine, uteroplacental and fetal amino-acid uptake rates and fetal amino-acid concentrations [AA]. Results were compared with normal pregnant ewe...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1053/plac.2000.0610
更新日期：2001-02-01 00:00:00
abstract：:Endotheliochorial placentas occur in orders from all four major clades of eutherian mammal. Species with this type of placenta include one of the smallest (pygmy shrew) and largest (African elephant) land mammals. The endotheliochorial placenta as a definitive form has an interhemal area consisting of maternal endothe...
journal_title：Placenta
pub_type： 杂志文章,评审
doi：10.1016/j.placenta.2012.02.008
更新日期：2012-05-01 00:00:00
abstract：:Human papillomavirus (HPV) are more prevalent in spontaneous abortions than elect abortions and preferentially infect the trophoblasts. Related to this, HPV type 16 has been shown to productively replicate in 3A trophoblasts in tissue culture. Extending these earlier studies, the described study addresses the issue wh...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2007.08.005
更新日期：2008-01-01 00:00:00
abstract：:Human endothelial dysfunction is a common feature in many diseases of pregnancy, such as gestational diabetes (GD). Metabolic changes include abnormal synthesis of nitric oxide (NO) and abnormal membrane transport of l-arginine and adenosine in primary cultures of human umbilical vein (HUVEC, macrovascular) and placen...
journal_title：Placenta
pub_type： 杂志文章,评审
doi：10.1016/j.placenta.2010.12.011
更新日期：2011-03-01 00:00:00
abstract：BACKGROUND:Previous studies have shown that pregnancies complicated by placentas with an isolated single umbilical artery (iSUA) are at increased risk for small-for-gestational-age (SGA) births. The etiology of SGA in this population, however, remains unknown. OBJECTIVE:The primary objective of this study was to evalu...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2017.09.001
更新日期：2017-11-01 00:00:00
abstract：INTRODUCTION:Visualization of the retroplacental clear space (RPCS) may provide critical insight into the development of abnormally invasive placenta (AIP). In this pre-clinical study, we characterized the appearance of the RPCS on magnetic resonance imaging (MRI) during the second half of gestation using a liposomal g...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2019.01.017
更新日期：2019-02-01 00:00:00
abstract：BACKGROUND:The introduction of the Solomon technique for the treatment of twin-twin transfusion syndrome (TTTS) increased placental exposure to laser energy. This study aims to identify the impact of power and energy used in laser treatment on placental tissue and pregnancy outcome. METHODS:Pictures of all dye-injecte...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2017.02.023
更新日期：2017-04-01 00:00:00
abstract：:Fetal growth restriction (FGR) is the failure of a fetus to reach its full genetic growth potential. It occurs in up to 8% of pregnancies, and after premature birth is the second leading cause of infant mortality and morbidity. There is no treatment currently available for FGR. Its primary cause, when not attributable...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2015.03.003
更新日期：2015-06-01 00:00:00
abstract：BACKGROUND:The human placenta extrudes a variety of extracellular vesicles (EVs) into the maternal blood daily. These vesicles may be crucial to the adaptation of the maternal cardiovascular and immune systems to pregnancy. Quantifying the EVs that are released in early gestation is important to our understanding of ho...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2018.10.009
更新日期：2018-12-01 00:00:00
abstract：:Alphafetoprotein (AFP) is detectable in maternal serum from around six weeks of gestation and is synthesised by the yolk sac and the fetal liver. The role of the placenta in the transport and possible synthesis of AFP is uncertain. The aim of this study was to investigate placental expression of AFP and the AFP recept...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2004.06.005
更新日期：2005-02-01 00:00:00
abstract：:Accumulation of advanced oxidation protein products (AOPPs) is prevalent in obesity, advanced maternal age, diabetes mellitus, and polycystic ovary syndrome. Alterations in the regulation and signaling of angiogenic pathways have been recognized as a link between these conditions and pre-eclampsia. To investigate the ...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2013.06.308
更新日期：2013-10-01 00:00:00
abstract：:Approximately 6 per cent of placentae of babies admitted to a special care paediatric unit show evidence of peripheral villous stem branch oedema. In more than half of these cases there is evidence of fibrinous vasculosis (FV) in truncal veins. The affected placentae are generally thick and of small diameter, often of...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/s0143-4004(83)80020-4
更新日期：1983-01-01 00:00:00
abstract：OBJECTIVE:Bioinformatics analysis indicated that the arrestin ARRDC3 was upregulated in placental tissue from patients with preeclampsia (PE). The study aimed to confirm the finding by examining placenta samples from women with and without early-onset PE and to investigate ARRDC3 roles in trophoblast function. METHODS...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2019.10.009
更新日期：2020-01-01 00:00:00
abstract：:Placental malaria (PM) causes placental insufficiency, leading to reduced birth weight (BW). Placental mtDNA copy number (mtDNA-CN) and relative telomere length (RTL) have been described as potential biomarkers for placental insufficiency and intrauterine growth restriction (IUGR). We investigated their associations w...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2019.03.005
更新日期：2019-05-01 00:00:00
abstract：:The IPL/TSSC3 gene is expressed nearly exclusively from the maternal allele, and its protein product acts to limit placental growth in mice. This protein specifically marks Type II trophoblast in the labyrinthine layer of the mouse placenta. To investigate mouse-human homologies, we carried out immunohistochemistry wi...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/s0143-4004(03)00130-9
更新日期：2003-09-01 00:00:00
abstract：:A long and productive history of studies at high altitude has demonstrated that chronic hypoxia plays a key role in the aetiology of intrauterine growth restriction (IUGR) and pre-eclampsia. Susceptibility to altitude-associated IUGR varies among high-altitude populations in relation to their duration of altitude expo...
journal_title：Placenta
pub_type： 杂志文章,多中心研究,评审
doi：10.1016/j.placenta.2004.01.008
更新日期：2004-04-01 00:00:00
abstract：:The trophoblastic and endodermal tissues of the guinea pig were cytophotometrically examined at two stages of development, either shortly after the primary trophoblastic giant cell layer had degenerated (Sansom and Hill, 1931) and the visceral yolk sac was starting to form (Kaufman and Davidoff, 1977) (c. 10.5 days p....
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/s0143-4004(81)80029-x
更新日期：1981-10-01 00:00:00
abstract：:Genomic imprinting, which results in the mono-allelic expression of certain genes in a parent of origin-dependent manner, represents a specialized form of gene regulation which may be vitally important for mammalian development. The mechanisms which underlie imprinting and the molecular nature of the imprint itself re...
journal_title：Placenta
pub_type： 杂志文章,评审
doi：10.1016/s0143-4004(05)80638-1
更新日期：1996-01-01 00:00:00
abstract：:There is accumulating evidence that deficient trophoblast invasion of the placental bed spiral arteries is crucial to the pathogenesis of pre-eclampsia and intrauterine growth restriction. However, the factors which regulate the process of trophoblast invasion remain unclear. We have investigated whether extravillous ...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1053/plac.1999.0427
更新日期：1999-11-01 00:00:00
abstract：INTRODUCTION:We have identified some critical aspects concerning umbilical cord tissue mesenchymal stem cells: the lack of standards for cell isolation, expansion and cryopreservation, the lack of unanimous opinions upon their multilineage differentiation potential and the existence of very few results related to the f...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2015.08.011
更新日期：2015-10-01 00:00:00
abstract：:Placenta accreta is a pregnancy complication characterized by the presence of life-threatening uteroplacental neovascularization. The factors involving its development are unknown. Vascular endothelial growth factor (VEGF), placenta growth factor (PlGF) and their receptors (VEGFR) have important roles in vascular remo...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2004.12.011
更新日期：2006-01-01 00:00:00
