ATP independent calcium transport and binding by basal plasma membrane of human placenta.

Abstract:

:The transport of large amounts of Ca2+ by the plasma membranes of human placental syncytiotrophoblast is essential to the mineralization of the growing fetal skeleton. We have investigated transport by the basal (fetal-facing) plasma membrane (BPM). Ca2+ was taken up by purified BPM vesicles in a time-dependent manner and equilibrium attained in approximately 60 min. The apparent equilibrium space was many fold higher than that determined using other substrates (e.g. leucine), suggesting that Ca2+ is concentrated or bound within the vesicles. The more rapid uptake and exit in the presence of A23187 indicates that membrane transport is rate limiting and that Ca2+ is internalized within the membrane space. The initial rate of uptake was approximately by measurement during the first 2 s of incubation. Concentration dependence data were fit to a Michaelis-Menten model with one saturable site and diffusion (Km = 12 microM; Vmax = 4 nmol/min/mg; KD = 39 nmol/min/mg/mM). Saturable Ca2+ binding (Kd = 16 microM; Bmax = 3.4 nmol/mg) was of lower capacity than previously observed for microvillous membrane.

journal_name

Placenta

journal_title

Placenta

authors

Kamath SG,Haider N,Smith CH

doi

10.1016/s0143-4004(05)80451-5

subject

Has Abstract

pub_date

1994-02-01 00:00:00

pages

147-55

issue

2

eissn

0143-4004

issn

1532-3102

pii

S0143-4004(05)80451-5

journal_volume

15

pub_type

杂志文章

相关文献

PLACENTA文献大全
  • Nitric oxide synthase activity in placentae from women with pre-eclampsia.

    abstract::The syncytiotrophoblast (ST) cell layer of the human villous placenta expresses nitric oxide (NO) synthase. Because NO is a potent relaxant of vascular smooth muscle and inhibitor of platelet activity, we postulated that exaggerated intervillous aggregation of platelets and reduced fetoplacental blood flow in pre-ecla...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/0143-4004(95)90013-6

    authors: Conrad KP,Davis AK

    更新日期:1995-12-01 00:00:00

  • RANKL promotes the growth of decidual stromal cells in an autocrine manner via CCL2/CCR2 interaction in human early pregnancy.

    abstract:OBJECTIVE:Receptor-activator of NF-κB ligand (TNFSF11, also known as RANKL) and its receptor RANK are essential regulators on bone remodeling, mammary gland development and hormone-associated breast cancer development. However, the expression pattern and role of RANKL/RANK axis in decidual stromal cells (DSCs) are uncl...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2013.04.020

    authors: Meng YH,Li H,Chen X,Liu LB,Shao J,Chang KK,Du MR,Jin LP,Li MQ,Li DJ

    更新日期:2013-08-01 00:00:00

  • Fractional volume of placental vessels in women with diabetes using a novel stereological 3D power Doppler technique.

    abstract:OBJECTIVES:In maternal diabetes the placenta is large with abnormal vascular development and increased villous volume. We used a novel stereological 3D power Doppler ultrasound technique to investigate differences in-vivo in the placental fractional volume of power Doppler signal (FrVol-PD) between women with and witho...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2013.08.015

    authors: Jones NW,Deshpande R,Mousa HA,Mansell P,Raine-Fenning N,Bugg G

    更新日期:2013-11-01 00:00:00

  • IFPA Meeting 2013 Workshop Report III: maternal placental immunological interactions, novel determinants of trophoblast cell fate, dual ex vivo perfusion of the human placenta.

    abstract::Workshops are an important part of the IFPA annual meeting as they allow for discussion of specialised topics. At IFPA meeting 2013 there were twelve themed workshops, three of which are summarized in this report. These workshops related to various aspects of placental biology but collectively covered areas of placent...

    journal_title:Placenta

    pub_type:

    doi:10.1016/j.placenta.2013.11.009

    authors: Abumaree MH,Brownbill P,Burton G,Castillo C,Chamley L,Croy BA,Drewlo S,Dunk C,Girard S,Hansson S,Jones S,Jurisicova A,Lewis R,Letarte M,Parast M,Pehrson C,Rappolee D,Schneider H,Tannetta D,Varmuza S,Wadsack C,Wa

    更新日期:2014-02-01 00:00:00

  • Myofibroblast differentiation in the connective tissues of the amnion and chorion of term human fetal membranes-implications for fetal membrane rupture and labour.

    abstract::An area of the fetal membranes, within the rupture tear after spontaneous delivery at term, exhibits altered morphology compared to more distal sites. It is characterized by marked swelling of the amniotic and chorionic connective tissue layers, consistent with structural weakness, and a marked reduction of the thickn...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1053/plac.1999.0439

    authors: McParland PC,Taylor DJ,Bell SC

    更新日期:2000-01-01 00:00:00

  • Fused placentas: Till birth do us part.

    abstract::Twinning and placental fusion occur at a low frequency in species like humans and mice. They are linked to reduced birth weight and intertwin growth discrepancy with important consequences for perinatal survival. Despite this, little is known about the histological characteristics of the twin or fused placenta and how...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2020.10.036

    authors: Lopez-Tello J,Sferruzzi-Perri AN

    更新日期:2020-11-02 00:00:00

  • ED(27) trophoblast-like cells isolated from first-trimester chorionic villi are genetically identical to HeLa cells yet exhibit a distinct phenotype.

    abstract::ED(27) trophoblast-like cells were prepared from human chorionic villus samples obtained at 9 weeks gestation and have been grown continuously in vitro without phenotypic drift for nearly a decade. These cells express many trophoblast markers, including cytokeratin, placental alkaline phosphatase (PLAP), secretion of ...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1053/plac.2001.0749

    authors: Kniss DA,Xie Y,Li Y,Kumar S,Linton EA,Cohen P,Fan-Havard P,Redman CW,Sargent IL

    更新日期:2002-01-01 00:00:00

  • Amnion-derived cells as a reliable resource for next-generation regenerative medicine.

    abstract::The placenta is composed of the amnion, chorionic plate, villous and smooth chorion, decidua basalis, and umbilical cord. The amnion is a readily obtainable source of a large number of cells and cell types, including epithelium, mesenchyme, and endothelium, and is thus an allogeneic resource for regenerative medicine....

    journal_title:Placenta

    pub_type: 杂志文章,评审

    doi:10.1016/j.placenta.2019.06.381

    authors: Umezawa A,Hasegawa A,Inoue M,Tanuma-Takahashi A,Kajiwara K,Makino H,Chikazawa E,Okamoto A

    更新日期:2019-09-01 00:00:00

  • Morphometric assessment of the oxygen diffusion conductance in placentae from pregnancies complicated by intra-uterine growth restriction.

    abstract::The morphometric oxygen diffusive conductance (D(p)) of the placenta provides a measure of the efficiency of oxygen transfer between the mother and the developing fetus. Any change in the D(p)may point towards possible adaptation in the light of altered oxygen transfer. Placentae from normal (n=40) and small for gesta...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/s0143-4004(03)00044-4

    authors: Ansari T,Fenlon S,Pasha S,O'Neill B,Gillan JE,Green CJ,Sibbons PD

    更新日期:2003-07-01 00:00:00

  • Umbilical cord as an analytical matrix - A technical note.

    abstract::The umbilical cord (UC) connects the fetal blood supply to the placenta, so is exposed to all systemic endo- and xenobiotics. We have extensive experience using UC as an analytical matrix for detecting and/or quantitating drugs, chemicals and endogenous compounds. This technical note describes advantages (large amount...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2019.12.001

    authors: Price HR,Chehroudi C,Knight SJ,Smith AD,Lai D,Kim H,Wright TE,Coughtrie MW,Collier AC

    更新日期:2020-01-15 00:00:00

  • Monoclonal antibody defining a human syncytiotrophoblastic polypeptide immunologically related to mammalian retrovirus structural protein p30.

    abstract::Antigenic material previously detected in human placental trophoblastic cells by immunoperoxidase staining using a goat antiserum against the feline RD114 retrovirus structural protein p30 was isolated by immunochromatography from normal syncytiotrophoblast. The antigen was used to immunize mice, and of the monoclonal...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/s0143-4004(84)80001-6

    authors: Wahlström T,Nieminen P,Närvänen A,Suni J,Lehtovirta P,Saksela E,Vaheri A

    更新日期:1984-11-01 00:00:00

  • Villous trophoblast apoptosis is elevated and restricted to cytotrophoblasts in pregnancies complicated by preeclampsia, IUGR, or preeclampsia with IUGR.

    abstract::Human placental villi are surfaced by an outer multinucleated syncytiotrophoblast and underlying mononucleated cytotrophoblasts. Conflicting data have attributed one, or the other, of these villous trophoblast phenotypes to undergo enhanced apoptosis in complicated pregnancies, compared to term, normotensive pregnanci...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2012.01.017

    authors: Longtine MS,Chen B,Odibo AO,Zhong Y,Nelson DM

    更新日期:2012-05-01 00:00:00

  • Human predecidual stromal cells have distinctive characteristics of pericytes: Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors.

    abstract:INTRODUCTION:Human decidual stromal cells (DSCs) play a key role in maternal-fetal interactions. Precursors of DSCs (preDSCs) localize around vessels in both the endometrium and decidua. Previous studies suggested a relationship between preDSCs and pericytes because these cells share a perivascular location, alpha smoo...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2017.11.010

    authors: Muñoz-Fernández R,de la Mata C,Prados A,Perea A,Ruiz-Magaña MJ,Llorca T,Fernández-Rubio P,Blanco O,Abadía-Molina AC,Olivares EG

    更新日期:2018-01-01 00:00:00

  • Human placental adenosine receptor expression is elevated in preeclampsia and hypoxia increases expression of the A2A receptor.

    abstract::Placental hypoxia as a result of impaired trophoblast invasion is suggested to be involved in the pathophysiology of preeclampsia. Hypoxia is a potent stimulus for the release of adenosine, and the actions of adenosine are mediated through four adenosine receptors, A(1), A(2A), A(2B) and A(3). We investigated the pres...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2009.02.004

    authors: von Versen-Höynck F,Rajakumar A,Bainbridge SA,Gallaher MJ,Roberts JM,Powers RW

    更新日期:2009-05-01 00:00:00

  • Placental localization of 15-hydroxy-prostaglandin dehydrogenase in early and term human pregnancy.

    abstract::The human placenta possesses a large capacity for inactivation of prostanoids. This is due to the presence of large quantities of the NAD(+)-linked 15-hydroxy-prostaglandin dehydrogenase (PGDH type I; EC 1.1.1.141). In order to investigate whether a specific localization of PGDH is at the origin of the increasing plac...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/0143-4004(92)90037-t

    authors: Erwich JJ,Keirse MJ

    更新日期:1992-05-01 00:00:00

  • Altered uterine perfusion is involved in fetal outcome of diabetic rats.

    abstract::Maternal diabetes affects the development of the offspring by altering the uterine environment. We aimed to investigate the extent to which the blood flow (measured as Tissue Perfusion Units; TPU) to implantation sites and the expression of developmentally important genes in the offspring are affected by maternal diab...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2008.02.005

    authors: Zabihi S,Wentzel P,Eriksson UJ

    更新日期:2008-05-01 00:00:00

  • Expression of hypoxia-regulated genes and glycometabolic genes in placenta from patients with intrahepatic cholestasis of pregnancy.

    abstract:INTRODUCTION:Intrahepatic cholestasis of pregnancy (ICP) is a liver disorder unique to pregnancy that is associated with increased rates of fetal distress and demise. While acute hypoxia is believed to cause the pathophysiology of ICP, direct molecular evidence for this is lacking. Here, we analyzed expression of three...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2014.06.372

    authors: Wei W,Hu YY

    更新日期:2014-09-01 00:00:00

  • Specific and reversible interaction between pregnancy-associated plasma protein A and heparin.

    abstract::An interaction between heparin and circulating pregnancy-associated plasma protein A (PAPP-A) has been observed. This interaction could be reversed by the addition of the specific heparin antagonist protamine sulphate. In the presence of heparin, crossed immunoelectrophoretic analysis revealed an alteration in the ele...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/s0143-4004(83)80013-7

    authors: Westergaard JG,Hau J,Teisner B,Grudzinskas JG

    更新日期:1983-01-01 00:00:00

  • Uptake of microparticles by rat visceral yolk sac.

    abstract::The visceral yolk sac (VYS) is responsible for a major part of the amino acid nutrition of the early post-implantation rat embryo and possibly also at the fetal stage of gestation. The mechanism involves endocytic uptake of proteins by the tissue's epithelial cells followed by intralysosomal digestion to amino acids. ...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/0143-4004(77)90009-1

    authors: Pratten MK,Lloyd JB

    更新日期:1997-09-01 00:00:00

  • Placental morphology: from molecule to mother -- a dedication to Peter Kaufmann -- a review.

    abstract::Theories on the development of placental pathologies and insufficiencies are based on the assumption that the development of the placental villous trees, the invasion of extravillous trophoblast or the differentiation of the villous trophoblast is somehow dysregulated. One of the pioneers trying to answer these questi...

    journal_title:Placenta

    pub_type: 传,历史文章,杂志文章,评审

    doi:10.1016/j.placenta.2006.01.007

    authors: Huppertz B,Burton G,Cross JC,Kingdom JC

    更新日期:2006-04-01 00:00:00

  • Xanthine oxidase/dehydrogenase is present in human placenta.

    abstract::Xanthine dehydrogenase/oxidase (XDH, EC 1.1.1.204, XO, EC 1.2.3.2) produces uric acid, and in the oxidase form also generates the free radical superoxide. Previous reports failed to demonstrate XDH/XO activity in human placenta. Our objective was to determine evidence of XDH/XO in human placenta. We developed a cDNA p...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/s0143-4004(96)90061-2

    authors: Many A,Westerhausen-Larson A,Kanbour-Shakir A,Roberts JM

    更新日期:1996-07-01 00:00:00

  • Implantation and early placentation in the one-humped camel (Camelus dromedarius).

    abstract::The ovaries and uteri were removed from four pregnant camels on days 14, 25, 35 and 56 after ovulation. The day 14 and 25 uteri were perfuse-fixed with 3 per cent glutaraldehyde: 3 per cent paraformaldehyde whereas the day 35 and 56 specimens were opened dorsally for photography before biopsies of allantochorion attac...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/s0143-4004(96)90046-6

    authors: Skidmore JA,Wooding FB,Allen WR

    更新日期:1996-05-01 00:00:00

  • Placental cell release of transferrin: analysis by reverse haemolytic plaque assay.

    abstract::Recent demonstrations of transferrin (TF) mRNA in placental tissue raised the possibility that the placenta may serve as an extra-hepatic source of this iron-binding protein during development. In this study, we first confirmed these findings using cRNA probes for TF, and then adapted the TF reverse haemolytic plaque ...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/s0143-4004(05)80419-9

    authors: Boockfor FR,Harris SE,Barto JM,Bonner JM

    更新日期:1994-07-01 00:00:00

  • The placenta in leprosy.

    abstract::Eighty-one placentae from women with leprosy and 17 placentae from healthy controls were subjected to a detailed macroscopic, light microscopic, ultrastructural, immunopathological, microbiological and biochemical study. The placental morphology and immunohistology were normal, and there was no morphological evidence ...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/s0143-4004(84)80028-4

    authors: Duncan ME,Fox H,Harkness RA,Rees RJ

    更新日期:1984-05-01 00:00:00

  • Metabolite concentrations in human term placentae and their changes due to delayed collection after delivery.

    abstract::The present study was designed to quantify the major cellular metabolites in human placentae and their changes due to the confounding effect of time and subsequent hypoxia during sample collection using magnetic resonance spectroscopy ((1)H-and (31)P-MRS). The absolute placental concentrations of lactate, glucose, maj...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1053/plac.2002.0908

    authors: Serkova N,Bendrick-Peart J,Alexander B,Tissot van Patot MC

    更新日期:2003-02-01 00:00:00

  • Investigation of the rate-limiting step in the synthesis of acetylcholine by the human placenta.

    abstract::The uptake of [3H]choline and its conversion to [3H]acetylcholine were investigated in term human placental tissue in vitro. Although the net uptake of [3H]choline increased throughout a 45 min incubation period, intracellular [3H]choline levels reach a plateau after 2 min. There was a constant increase in [3H]acetylc...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/s0143-4004(84)80036-3

    authors: Leventer SM,Rowell PP

    更新日期:1984-05-01 00:00:00

  • Cullins in human intra-uterine growth restriction: expressional and epigenetic alterations.

    abstract::Intra-uterine growth restriction (IUGR) is defined by a restriction of fetal growth during gestation. It is a prevalent significant public health problem that jeopardizes neonatal health but also that can have deleterious consequences later in adult life. Cullins constitute a family of seven proteins involved in cell ...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2009.11.008

    authors: Gascoin-Lachambre G,Buffat C,Rebourcet R,Chelbi ST,Rigourd V,Mondon F,Mignot TM,Legras E,Simeoni U,Vaiman D,Barbaux S

    更新日期:2010-02-01 00:00:00

  • Characterization of human placental activity for transport of L-alanine, using brush border (microvillous) membrane vesicles.

    abstract::To characterize the placental activity for the transport of amino acids, the uptake of L-alanine was investigated by rapid membrane filtration using brush border membrane vesicles separated from the human placenta of early pregnant (12-13 gestational weeks) and late pregnant (37-38 gestational weeks) women. The uptake...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/0143-4004(92)90032-o

    authors: Iioka H,Hisanaga H,Moriyama IS,Akada S,Shimamoto T,Yamada Y,Ichijo M

    更新日期:1992-03-01 00:00:00

  • Uptake of 1-methyl-4-phenylpyridinium (MPP+) by the JAR human placental choriocarcinoma cell line: comparison with 5-hydroxytryptamine.

    abstract::The aim of this work was to characterize the uptake of 1-methyl-4-phenylpyridinium (MPP(+)) in the JAR human choriocarcinoma cell line. As JAR cells, as well as the placenta, express the neuronal serotonin transporter (SERT), a comparison between the uptake of (3)H-MPP(+) and (3)H-serotonin ((3)H-5HT) was made. Specif...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1053/plac.2002.0917

    authors: Martel F,Keating E

    更新日期:2003-04-01 00:00:00

  • The increased endotoxin-sensitivity of pregnant rats, as reflected by glomerular ecto-ADP-ase activity, is not dependent on the presence of decidual cells.

    abstract::In the present study the possible role of decidual cells in the pregnancy-associated increased sensitivity of glomerular ecto-ADP-ase to endotoxin was investigated. Early (day 5) pregnant (E-Pr; n = 10), pseudopregnant (E-PSP; n = 10), (day 5), pseudopregnant rats with a decidualized uterus (E-DEC; n - 10), as well as...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/s0143-4004(96)80013-0

    authors: Faas MM,Schuiling GA,Baller JF,Valkhof N,Bakker WW

    更新日期:1996-03-01 00:00:00