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Chemical profile- and pharmacokinetics-based investigation of the synergistic property of platycodonis radix in traditional Chinese medicine formula Shengxian decoction.

Abstract：

ETHNOPHARMACOLOGICAL RELEVANCE:To investigate the synergistic property of Platycodonis radix (PG) in a classic traditional Chinese medicine (TCM) prescription Shengxian decoction (SXT) by combining chemical profile with pharmacokinetic analysis strategy. The synergized prescription consisted of Astragali radix, Anemarrhenae rhizoma, Bupleuri radix, and Cimicifuage rhizoma. MATERIALS AND METHODS:Ultra-performance liquid chromatography/quadruple time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was employed to investigate the chemical fingerprints of SXT and decreased SXT (SXT that removed Platycodonis radix, SXT-PG). A reliable LC-MS/MS method was developed to examine the pharmacokinetics of 9 marker compounds (including formononetin, calycosin-7-O-β-d-glucoside, ononin, caffeic acid, isoferulic acid, mangiferin, timosaponin E1, timosaponin B-II and timosaponin B) following oral administration of SXT and SXT-PG in rats. Both in vitro chemical profiles and in vivo pharmacokinetic parameters differences between SXT and SXT-PG were conducted. RESULTS:By using UPLC-Q-TOF/MS method, a total of 25 compounds identified from SXT, including 13 triterpenoids, 5 caffeinic derivatives, 4 isoflavonoids and 3 xanthone glycosides. Comparing the chemical fingerprints between SXT and decreased SXT did not reveal significant difference in the chemical profile of other four TCMs. The improved pharmacokinetic profiles of mangiferin, timosaponin E1, timosaponin B-II and timosaponin B were found in SXT group, suggesting the quicker distribution and more effective absorption, when compared with those in the SXT-PG group. CONCLUSIONS:These results indicated that PG did not increase the dissolution of synergized prescription when co-decocting, but guided the synergized prescription to target location, reflecting the courier role of PG, which was in line with the clinical principle of TCM. It also established a useful method for TCM synergistic property research.

journal_name

J Ethnopharmacol

journal_title

Journal of ethnopharmacology

authors

Zhang F,Zhan Q,Gao S,Dong X,Jiang B,Sun L,Tao X,Chen WS

doi

10.1016/j.jep.2014.01.033

subject

Has Abstract

pub_date

2014-03-28 00:00:00

pages

497-507

issue

eissn

0378-8741

issn

1872-7573

pii

S0378-8741(14)00080-4

journal_volume

152

pub_type

杂志文章

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Chemical profile- and pharmacokinetics-based investigation of the synergistic property of platycodonis radix in traditional Chinese medicine formula Shengxian decoction.