Abstract:
:Sphere-forming assays are widely used for the propagation, characterization and manipulation of adult brain-derived stem- and progenitor cells. However despite the broad application of this cell culture system in neural stem cell- and brain tumor research, no standardized protocols exist. Variations in experimental procedures not only concern the use of media components but also cell density, the number of passages the cells are propagated before analysis and, in cases where the neurogenic or gliogenic potential of the cells is investigated, the duration that the cells are allowed to differentiate. The latter deserves consideration because the proportion of differentiated cells obtained at the endpoint of the experiment depends not only on the absolute number of cells that differentiate at a given time, but also on the number of cell divisions prior to differentiation and the rate of cell death in the cultures. In the present study we describe a fast and simple differentiation protocol to investigate the pro-neurogenic potential of soluble factors added to subventricular zone (SVZ)-derived neurospheres. The assay relies on the use of primary neurospheres and very short differentiation times, thereby largely excluding the contribution of cell proliferation and cell death to the results. We use this modified assay to test the consequence of pharmacological inhibition of the EGF receptor-, Erk1/2-, Protein Kinase B/AKT-, and Sonic Hedgehog-pathways on neuronal differentiation of SVZ-neurosphere cultures.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Schramm J,Schulte Ddoi
10.1016/j.neulet.2014.01.003subject
Has Abstractpub_date
2014-03-06 00:00:00pages
69-74eissn
0304-3940issn
1872-7972pii
S0304-3940(14)00021-4journal_volume
562pub_type
杂志文章abstract::IL-1beta is a proinflammatory cytokine that contributes to psychological stress responses and has been implicated in various psychiatric disorders most notably depression. Preclinical studies also demonstrate that IL-1beta modulates anxiety- and fear-related behaviors, although these findings are difficult to assess b...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.03.068
更新日期:2009-05-29 00:00:00
abstract::Galanin (GAL) is distributed in sympathetic nerves in the cat, and exogenous GAL inhibits cardiac vagal action and lowers blood pressure in this species. This study on anaesthetised cats compares the effects on cardiac vagal action and blood pressure of human, rat and porcine GAL. Human GAL has only recently been sequ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90659-u
更新日期:1992-02-17 00:00:00
abstract::The Gallyas-Braak silver impregnation method revealed neurons containing well-defined intraneuronal granules in both Alzheimer's disease and normal control brains. The granules were immunostained prominently with the Alz-50 antibody and, to a lesser degree, with the tau-2 antibody, but not with other anti-tau antibodi...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(98)00867-2
更新日期:1998-12-18 00:00:00
abstract::It is widely held that transdiaphragmatic pressure is a reliable index of the extent of central activation of the diaphragm but the maximal voluntary transdiaphragmatic pressure is lower during inspiratory than expulsive efforts. To determine whether the diaphragm is fully activated during the two manoeuvres supramaxi...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(88)90481-8
更新日期:1988-06-17 00:00:00
abstract::Cancer patients with bone metastases often suffer breakthrough pain. However, little progress has been made in the treatment of breakthrough pain and its associated mechanism(s) in the patient with cancer due to lacking of resembling and predictive animal models. We previously have demonstrated that endothelin-1 plays...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2016.01.053
更新日期:2016-03-23 00:00:00
abstract::We assessed whether tolerance develops to the antinociceptive effect of intrathecal (i.t.) orphanin FQ, the endogenous ligand of the orphan opioid-like receptor, and whether there is a cross-tolerance between the antinociceptive effect of i.t. orphanin FQ and the mu opioid receptor agonist morphine. Orphanin FQ admini...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(97)13401-2
更新日期:1997-02-14 00:00:00
abstract::Immunolocalization of 14-3-3 protein isoforms in relation to Pick bodies in Pick body disease (PBD) brains was investigated. Weakly granular immunoreactivity of 14-3-3 proteins was found in neurons in control subjects and in Pick body disease brains. In addition to this granular immunoreactivity, many Pick bodies were...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.08.079
更新日期:2004-11-23 00:00:00
abstract::Projection of the vagal C-afferent to the insular cortex was examined in rats. Electrical activation of the C-afferent resulted in two cortical field potentials in the anterior insula. They were independent of each other and neither of them was attributed to the specific thalamic projection. These results imply that v...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90827-t
更新日期:1992-12-14 00:00:00
abstract::The role of intracellular amyloid beta (iAbeta) in Alzheimer's disease (AD) initiation and progression attracts more and more attention in recent years. To address whether iAbeta induces early alterations of electrophysiological properties in cultured human primary neurons, we delivered iAbeta with adeno-virus and mea...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.07.031
更新日期:2009-10-25 00:00:00
abstract:BACKGROUND:Diverse physiological and pathological functions of 27-hydroxycholesterol (27-OHC) were proved. However, cytotoxicity and potential influence of 27-OHC on cholesterol metabolism in neurons are unclear. DESIGN AND METHODS:In the vitro co-culture system, SH-SY5Y cells and C6 cells were applied to explore the ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2016.08.056
更新日期:2016-10-06 00:00:00
abstract::Recent imaging studies have reported the projection of semicircular canal signals onto wide regions of the cerebral cortex but little is known about otolith projections onto the cerebral cortex. We used functional magnetic resonance imaging (fMRI) to investigate the activation of the cortex by loud clicks that selecti...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2007.06.036
更新日期:2007-08-09 00:00:00
abstract::The retrograde transport of receptor-bound opiate was markedly enhanced in the vagus nerves of rats housed for 25 days in an atmosphere of ethanol vapor. This increase disappeared after 8 days of withdrawal as did the animal preference for ethanol. In contrast, the axonal transport of muscarinic receptors in sciatic n...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(87)90525-8
更新日期:1987-06-26 00:00:00
abstract::Normal aging is associated with chronic oxidative stress. In the basal ganglia, oxidative stress may contribute to the increased risk of Parkinson's disease in the elderly. Neurons are thought to actively utilize compensatory defense mechanisms, such as heat shock proteins (HSPs), to protect from persisting stress. De...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2010.01.049
更新日期:2010-03-19 00:00:00
abstract::We previously obtained evidence that in rats the neurons in the suprachiasmatic nucleus (SCN) receiving retinal neural inputs may be involved in the regulation of glucose metabolism. In this study we examined whether the SCN neurons containing vasoactive intestinal peptide (VIP)-like immunoreactive substance (VIP neur...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)91103-7
更新日期:1994-07-04 00:00:00
abstract::Typical histopathological findings of multiple system atrophy (MSA) have been observed in motor related cortices, in addition to the common areas of the striatum, substantia nigra, olivopontocerebellar pathways, the intermediolateral cell columns of the spinal cord, and the cerebellum. The purpose of this study is to ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2011.07.015
更新日期:2011-09-08 00:00:00
abstract::This report concerns an ultrastructural study of the synapses present on the surface of somata of normal-looking Betz cells in the fifth layer of motor cortex of seven patients with amyotrophic lateral sclerosis (ALS). Specimens from 12 age-matched, neurologically normal control individuals were included for compariso...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(99)00374-2
更新日期:1999-06-11 00:00:00
abstract::When a tone-burst train with a short inter-burst interval is lateralized to the left and is followed by another tone-burst train lateralized to the right, listeners report a smooth shift of tone bursts along the intracranial axis from left to right (known as auditory saltation). When a sequence of tone bursts with a c...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.11.020
更新日期:2010-01-14 00:00:00
abstract::Many researchers have investigated the variability of discrete goal-directed movements. However, a possible time-dependent property for them has been ignored. We investigated the time-dependent property of the kinematic variability in human rapid goal-directed movements by using fractal analysis. The variability of th...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)01779-7
更新日期:2001-05-25 00:00:00
abstract::We have previously shown that D-cycloserine displaces [3H]glycine binding to a recognition site with properties consistent with an N-methyl-D-aspartate (NMDA) receptor modulatory site. Additionally, D-cycloserine positively modulates the NMDA receptor as evidenced by its dose-dependent enhancement of [3H]1-[1-(2-thien...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(89)90379-0
更新日期:1989-03-13 00:00:00
abstract::In control samples, intense acoustic "go" stimuli accelerate the central and peripheral motor processes that compose simple reaction time movements. The goal of the current study was to determine whether movements that are initiated to intense acoustic cues facilitate simple reaction times in (1) adults with chronic s...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.01.041
更新日期:2009-03-13 00:00:00
abstract::The effect of oleamide on apoptosis was investigated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction assay, DNA staining assay with propidium iodide and caspase-3 activity analyses. The present results showed that oleamide significantly attenuated the cell death and nuclear condensation...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(02)00460-3
更新日期:2002-08-09 00:00:00
abstract::The effect of acute phenylethanolamine-N-methyltransferase (PNMT) inhibition on rat brain catecholamine concentrations was studied. All PNMT inhibitors tested were effective in depleting hypothalamic and brain stem epinephrine. Norepinephrine concentrations were unaffected; however, dopamine levels were increased in a...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(80)90235-9
更新日期:1980-10-20 00:00:00
abstract::The effects of Sendai, a paramyxovirus, on the functional activity of 3 cell types, have been studied in vitro to establish whether a virus alone can cause pathophysiological changes. Neuronal cells are depolarized and suffer a loss of excitability which was attributed to an increase in membrane conductance. Spontaneo...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(82)90323-8
更新日期:1982-04-26 00:00:00
abstract::Tumor related contralateral motor deficits complicate preoperative functional magnetic resonance imaging (fMRI). In plegic patients the localization of the sensorimotor cortex is often impossible. In this context we developed a clinical fMRI protocol dedicated to patients with motor deficits using the unaffected ipsil...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(00)01045-4
更新日期:2000-05-12 00:00:00
abstract:RATIONALE:Some investigations have shown that the glutamate receptors play a critical role in cognitive processes such as learning and anxiety. OBJECTIVES:The possible involvement of the cholinergic system of the dorsal hippocampus in the anxiolytic-like response induced by MK-801, NMDA receptor antagonist, was invest...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2011.08.009
更新日期:2011-11-14 00:00:00
abstract::Neurons of the medial septum-nucleus of the diagonal band of Broca (vertical limb) area were identified as septo-hippocampal neurons by antidromic electrical activation in anesthetized rats. A large majority of these presumably cholinergic neurons could be excited by the iontophoretic application of acetylcholine or c...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(83)90126-x
更新日期:1983-12-23 00:00:00
abstract::A cDNA clone encoding a novel protein (Cer-1) was isolated from a mouse cerebellar cDNA library. The deduced amino acid sequence of Cer-1 is not homologous to other sequences in the protein sequence data base. RNA blot analysis suggests that expression of Cer-1 RNA occurs selectively in the adult cerebellum and in pur...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(96)12628-8
更新日期:1996-05-10 00:00:00
abstract::We studied the influence of caffeine treatment (50 mg/kg for 3 doses) on catecholamine utilization and adrenergic receptor binding in female rats. Caffeine enhanced the reduction in forebrain norepinephrine levels following alpha-methyl-p-tyrosine without altering the reduction in dopamine levels. Caffeine reduced the...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(82)90052-0
更新日期:1982-07-20 00:00:00
abstract::Here we examine hypothesis that short-term peripheral ZnSO(4)-induced anosmia can produce effects on c-fos expression within spinal cord and caudal medulla in male Wistar rats (n=4). Fos-like-immunoreactive cells revealed by avidin-biotin-peroxidase method show a significant bilateral increase in the nucleus proprius ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)01623-8
更新日期:2001-03-30 00:00:00
abstract::Both muscarinic and nicotinic acetylcholine (ACh) receptors are known to be present on the surface of lymphocytes. We have shown that variable amounts of ACh are detectable in the blood of various mammals including humans, and a major portion of blood ACh is localized in circulating mononuclear leukocytes in humans. I...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(98)00921-5
更新日期:1999-01-08 00:00:00