Abstract:
BACKGROUND:Diverse physiological and pathological functions of 27-hydroxycholesterol (27-OHC) were proved. However, cytotoxicity and potential influence of 27-OHC on cholesterol metabolism in neurons are unclear. DESIGN AND METHODS:In the vitro co-culture system, SH-SY5Y cells and C6 cells were applied to explore the potential cytotoxicity of 27-OHC. MTT assay was used to detect the cell proliferation. Cell vitality was measured by using the Annexin-FITC/PI test. Immunofluorescence technique was applied to observe the changes of mitochondria membrane potential. The expression of mRNA and protein (including SREBP-1, HMGCR, LXR-α, ABCA1) were measured by real-time PCR and western blot method respectively. RESULTS:27-OHC induced apoptosis in co-cultured SH-SY5Y cells and C6 cells. 27-OHC treatment significantly inhibited cell viability and proliferation (p<0.05). Compared with control group, 27-OHC caused the reduction of mitochondrial membrane potential (p<0.05). Additionally, the mRNA and protein expression of cholesterol synthesis-related factors, such as SREBP-1, HMGCR, were down-regulated (p<0.05), while the mRNA and protein expression of cholesterol transport-related factors (LXR-α, ABCA1) were up-regulated (p<0.05). CONCLUSIONS:Cytotoxicity and cholesterol metabolism disorder induced by 27-OHC may contribute to the pathogenesis of neurodegenerative disease.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Wang H,Yuan L,Ma W,Han J,Lu Y,Feng L,Xiao Rdoi
10.1016/j.neulet.2016.08.056subject
Has Abstractpub_date
2016-10-06 00:00:00pages
209-17eissn
0304-3940issn
1872-7972pii
S0304-3940(16)30656-5journal_volume
632pub_type
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