Abstract:
:Human herpesviruses (HHVs) are involved in the pathogenesis of different types of uveitis. Cytokine response plays an important role in virus-induced immunopathology. This study aimed to investigate the incidence of HHVs in aqueous humor samples of immunocompetent patients with suspected viral uveitis and cytokine (IL-10, IL-6, and IFN-γ) expression profiling. Forty-seven aqueous humor samples were collected from immunocompetent patients with viral uveitis. Samples were assayed for HHV-1 to HHV-8 by in-house real-time polymerase chain reactions. IL-6, IL-10, and IFN-γ were quantified with a cytometric bead array. Relations between viral detection, cytokine profiles, and clinical data were studied. At least one viral genome was detected in 21 aqueous humor samples analyzed. Varicella-zoster virus (VZV) was detected in 14 of the positive samples, cytomegalovirus (CMV) in 8, HSV-1 in 1, Epstein-Barr virus (EBV) in 4, and HHV-6 in 2. More than one viral genome was detected in seven aqueous humor samples. Aqueous humor samples positive for HHV-DNA contained significant levels of IL-6, IL-10, and IFN-γ, compared to HHV-DNA negative samples. High levels of IL-6 were detected in patients with CMV-DNA in their aqueous humor samples. Significantly higher levels of IL-10 and IFN-γ were found in positive samples for VZV, EBV, and HHV-6 DNA than in negative aqueous humor ones. VZV was the principal etiologic agent of uveitis in this Tunisian series, with CMV the second most common agent. Knowledge of immunoregulatory interactions and dynamic changes in viral uveitis may be a key to understand the pathogenesis leading to more-effective treatments.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Nahdi I,Abdelwahed RB,Boukoum H,Bressollette-Bodin C,Attia S,Yahia SB,Fisson S,Khairallah M,Aouni Mdoi
10.1002/jmv.23708subject
Has Abstractpub_date
2013-12-01 00:00:00pages
2079-86issue
12eissn
0146-6615issn
1096-9071journal_volume
85pub_type
杂志文章abstract::West Nile virus (WNV) activity has fluctuated in the south-central United States since its introduction. Seasonal outbreaks are common, with three in Oklahoma during 2003, 2007, and 2012. Morbidity and mortality rates vary during each outbreak. Long-term neurologic sequelae in association with West Nile virus disease ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24235
更新日期:2015-10-01 00:00:00
abstract::Until recently, human cytomegalovirus (hCMV) infection and anti-CMV treatment in transplanted patients have been monitored essentially by pp65 antigenemia, which is time-consuming and requires experienced operators. For the last two years, pp65 antigenemia levels have tended to be lower than previously in our laborato...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20326
更新日期:2005-05-01 00:00:00
abstract::There are few data describing the comprehensive identification in and influence of newly identified respiratory viruses on asthma exacerbations. Most studies focus on inpatients. In this preliminary study, the point prevalence and the associations of picornavirus species described recently and human bocavirus (HBoV) w...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21819
更新日期:2010-08-01 00:00:00
abstract::Individual specific antigenic rubella virus (RV) structural proteins are required for accurate serological diagnosis of acute and congenital rubella infections as well as rubella immune status. The RV envelope glycoprotein E1 is the major target antigen and plays an important role in viral-specific immune responses. T...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890440214
更新日期:1994-10-01 00:00:00
abstract::Human papillomavirus (HPV) is a carcinogenic agent responsible for tumor development in many sexually involved tissues. We present a survey on the prevalence of HPV infection in a risk population for sexually transmitted infections (STIs). The studied population was formed by 125 STI clinic attendees, who took part in...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24746
更新日期:2017-06-01 00:00:00
abstract::Co-infection with hepatitis delta virus (HDV) and hepatitis B virus (HBV) has been shown to be associated with a more severe form of acute and chronic hepatitis. Cloning and expression of recombinant HDV antigen (rHDAg) in Escherichiacoli are described. Using purified rHDAg, a cost-effective indirect anti-HDV enzyme-l...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23212
更新日期:2012-03-01 00:00:00
abstract::Thirty-nine children without previous cytomegalovirus (CMV) infection received blood transfusion in the National Taiwan University Hospital. The overall transfusion-acquired CMV infection rate was 36% (14/39). Donor CMV seropositive rate was 70%. None of the nine children who had received seronegative blood became inf...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890360110
更新日期:1992-01-01 00:00:00
abstract::Guinea pig infection with a highly passaged XJ prototype strain of Junín virus by the intramuscular route (IM) was carried out in order to study viral tropism modification in this host. The neurotropism of this strain was demonstrated by viral isolation, meningitis, and by the presence of Junín antigens as shown by im...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890150208
更新日期:1985-02-01 00:00:00
abstract::Human herpesvirus 6 (HHV-6) prototype isolate GS is a newly identified lymphotropic herpesvirus and several subsequent herpes isolates were recognized as HHV-6 by their hybridization to a HHV-6(GS) DNA probe pZVH14. DNA restriction analysis and in vitro tropism studies show that HHV-6 isolates can be divided into two ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890370403
更新日期:1992-08-01 00:00:00
abstract::Knowledge of the nucleotide sequence in the region of the putative VP1/2A junction of the Hepatitis A virus (HAV) genome has enabled differentiation of HAV strains and their classification into seven genotypes, in some of which sub-genotypes A and B can be defined. A 168 base segment encompassing the putative VP1/2A j...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1023
更新日期:2001-06-01 00:00:00
abstract::Saffold viruses (SAFV) have been discovered recently and they are classified into Theilovirus species in genus Cardiovirus in the Picornaviridae family. SAFV, especially those belonging to the genotype 2, have been difficult to propagate in laboratory cell lines. This study describes the successful isolation of an eff...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23352
更新日期:2012-09-01 00:00:00
abstract::Nasopharyngeal carcinoma is closely associated with Epstein-Barr virus (EBV) and the EBV encoded latent membrane protein-1 expression (LMP1) is commonly found in the tumour cells. LMP1 has been shown to be involved in modulation of cell growth in B cells but the biological properties of LMP1 expression in nasopharynge...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2112
更新日期:2002-01-01 00:00:00
abstract::Thirty-two cerebrospinal fluid (CSF) samples from eighteen patients with confirmed herpes simplex encephalitis (HSE) were assayed by an indirect enzyme-linked immunosorbent assay (ELISA) for the presence of viral antigens. The results are expressed as an antigen ratio distinguishing between herpes simplex virus (HSV) ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890210209
更新日期:1987-02-01 00:00:00
abstract::To characterize the state of dual infection with hepatitis B virus (HBV) and hepatitis C virus (HCV), HBV-DNA and HCV-RNA levels were determined by spot hybridization or polymerase chain reaction in the sera of patients who were positive for both hepatitis B surface antigen and HCV antibody. Among 27 patients who show...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890450222
更新日期:1995-02-01 00:00:00
abstract::Chronic HCV infection has emerged as a complex multifaceted disease with manifestations extending beyond the liver. HCV plays a direct role in glucose metabolism leading to both insulin resistance and type 2 diabetes. To evaluate the changes in the glycemic state following Sofosbuvir-based treatment regimens in diabet...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24897
更新日期:2017-12-01 00:00:00
abstract::The seroprevalence of viral hepatitis in healthcare workers has important public health implications. To assess the risk factors for the acquisition of viral hepatitis in an unvaccinated cohort from an hyperendemic region, 567 healthcare workers from a large hospital in the capital of the Republic of Yemen were interv...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20126
更新日期:2004-08-01 00:00:00
abstract::The transmission of the severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) can occur through airborne route, in addition to contaminated surfaces and objects. In hospitals, it has been confirmed by several studies that SRAS-CoV-2 can contaminate surfaces and medical equipment especially in hospitals dedicat...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26819
更新日期:2021-01-26 00:00:00
abstract::A random peptide library of heptamers displayed on the surface of M13 bacteriophage was used to identify specific epitopes of antibodies in pooled sera of swine naturally infected by Nipah virus. The selected heptapeptides were aligned with protein sequences of Nipah virus and several putative epitopes were identified...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20249
更新日期:2005-01-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) play a central role in tumor invasion and metastasis. Increased expression of MMPs occurs during development of hepatocellular carcinoma (HCC) in humans following infection with hepatitis B virus (HBV). Woodchucks are used as an animal model for hepadnavirus-induced HCC. All woodchucks...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23571
更新日期:2013-07-01 00:00:00
abstract::Recently, human platelet antigens (HPAs) polymorphisms are found to play a role in susceptibility to hepatitis C virus (HCV) infection and fibrosis progression. The aim of the current study was to evaluate the possible association between the HPAs polymorphisms with liver fibrosis progression in HCV patients. HPAs pol...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25423
更新日期:2020-01-01 00:00:00
abstract::Many patients with mixed cryoglobulinemia and chronic HCV infection experience symptoms, such as dyspnea, which sometimes do not seem to indicate the involvement of the liver but rather the symptoms of heart failure. To our knowledge, there has been no other study evaluating the serum levels of N-terminal pro-brain na...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21636
更新日期:2010-02-01 00:00:00
abstract::Comparison of RT-PCR assays established in house at various places revealed that laboratories could differ in sensitivity by as much as 1,000-fold in terms of the ability to detect measles virus sequences in clinical samples. The study indicates that PCR findings, positive or negative, are questionable if they are not...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10371
更新日期:2003-05-01 00:00:00
abstract::To determine whether unconventional pathogens causing subacute spongiform encephalopathy may be present in blood products, a newly developed hepatitis B vaccine and a widely used blood product were injected into mice and rats. As only a few aged mice in the test and the control groups showed spongiform encephalopathic...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890160103
更新日期:1985-05-01 00:00:00
abstract::Influenza is a public health burden, responsible for more than half a million deaths worldwide each year and explosive outbreaks in-hospital care units. At present, little is known about clinical characteristics and outcomes with nosocomial influenza infection. To assess clinical characteristics and outcome between no...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25652
更新日期:2020-08-01 00:00:00
abstract::Paired sera, saliva and cervicovaginal secretions from 17 HTLV-I-infected women (19-75 yr) were tested for total IgA and IgG, for IgA and IgG to the immunodominant region gp46/175-Pro-199, for serum IgG to the neutralizing domains gp46/ 190-Pro-199 and gp46/190-Ser-199, or for tax-rex proviral HTLV-DNA. Serum antibodi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9071(199609)50:1<42::AID-JMV9>3
更新日期:1996-09-01 00:00:00
abstract::Eastern equine encephalitis (EEE) is rare, but the most severe of the mosquito-borne encephalitides in the United States with a high case fatality rate of 30%. Here, we present a patient with EEE. EEE virus causes sporadic human disease in the Eastern parts of the United States, but the case we describe was a Scottish...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21379
更新日期:2009-02-01 00:00:00
abstract::The effect of protamine, atropine, selenocystamine, taxifolin, and catechin on the infectivity and antigenicity of the cell culture-adapted hepatitis A virus (HAV) strain CF 53 was studied. The toxicity on uninfected PLC/PRF/5 cells was examined for each antiviral compound by morphological and biochemical methods, in ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890220108
更新日期:1987-05-01 00:00:00
abstract::Cells from a leiomyosarcoma tumor (LMS-1) from a patient with the acquired immunodeficiency syndrome (AIDS) were explanted, cultured in vitro, and studied by phase-contrast microscopy for morphologic and growth characteristics, immunostaining for cell markers, EBER in situ hybridization and polymerase chain reaction f...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::This study was designed to determine the prognostic value of erythrocyte adenosine deaminase (ADA) as a possible indicator of progression to AIDS, and compare this with other known cellular and serological markers. At the end of a 3-year study, a cohort of 114 human immunodeficiency virus-1 (HIV-1) seropositive intrav...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章
doi:10.1002/jmv.1890450216
更新日期:1995-02-01 00:00:00
abstract::We report a male caucasian German pediatric patient of no Arab or Mediterranean ancestry with virus associated CNS lesions in Griscelli's syndrome (GS; McKusick No. 214450). The boy presented with recurrent infections, and meningitis with subsequent progressive signs of increased intracranial pressure leading to death...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199711)53:3<306::aid-jmv21
更新日期:1997-11-01 00:00:00