Abstract:
:An ordered cellulose film scaffold, termed a nematic ordered cellulose (NOC) template, had unique surface properties and successfully induced the establishment of a three-dimensional (3D), hierarchical structure of epidermal cells by cell attachment and subsequent culture. Initially, the scaffold surface properties were characterized through contact angle measurements and atomic force microscopy to evaluate appropriate hydrophobicity and orientation of molecular chains for 3D culture. The template surfaces exhibited higher hydrophobicity, in the range of 70-75°, than usual cellulose films and appeared suitable for surface cell adhesion. In fact, epidermal cells successfully attached and proliferated favorably on the NOC templates, similar to development in normal culture flasks. Furthermore, the NOC film, as a semipermeable template, was also employed to allow 3D proliferation of epidermal cell layers in the perpendicular direction. The template proved to be suitable as a 3D cell culture device, resulting in the proposal that the construction processes of these 3D cell layers followed the basic concept of skin formation.
journal_name
Biofabricationjournal_title
Biofabricationauthors
Seyama T,Suh EY,Kondo Tdoi
10.1088/1758-5082/5/2/025010subject
Has Abstractpub_date
2013-06-01 00:00:00pages
025010issue
2eissn
1758-5082issn
1758-5090journal_volume
5pub_type
杂志文章相关文献
Biofabrication文献大全abstract::Laser-assisted bioprinting (LaBP) allows the realization of computer-generated 3D tissue grafts consisting of cells embedded in a hydrogel environment. In this study, human adipose-derived stem cells (hASCs) were printed in a free-scalable 3D grid pattern by means of LaBP. We demonstrate that neither the proliferation...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/3/1/015005
更新日期:2011-03-01 00:00:00
abstract::Cell-imprinting technology is a novel method for directing stem cell fate using substrates molded from target cells. Here, we fabricated and studied cell-imprinted substrates for neural priming in human adipose-derived stem cells in the absence of chemical cues. We molded polydimethylsiloxane (PDMS) silicone substrate...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/abc66f
更新日期:2020-10-30 00:00:00
abstract::To overcome the drawbacks of in vitro liver testing during drug development, numerous liver-on-a-chip models have been developed. However, current liver-on-a-chip technologies are labor-intensive, lack extracellular matrix (ECM) essential for liver cells, and lack a biliary system essential for excreting bile acids, w...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aaf9fa
更新日期:2019-01-16 00:00:00
abstract::In the pharmaceutical industry, new drugs are tested to find appropriate compounds for therapeutic purposes for contemporary diseases. Unfortunately, novel compounds emerge at expensive prices and current target evaluation processes have limited throughput, thus leading to an increase of cost and time for drug develop...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/4/3/035001
更新日期:2012-09-01 00:00:00
abstract::In the body, cells are surrounded by an interconnected mesh of insoluble, bioactive protein fibres to which they adhere in a well-controlled manner, embedded in a hydrogel-like highly hydrated matrix. True morphological and biochemical mimicry of this so-called extracellular matrix (ECM) remains a challenge but appear...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/2/024106
更新日期:2014-06-01 00:00:00
abstract::Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different p...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa8114
更新日期:2017-08-02 00:00:00
abstract::Omentum-based matrices fabricated by decellularization have the potential to serve as autologous scaffolds for tissue engineering. Transplantation of such scaffolds prepared from the patient's own biomaterial may reduce the immunogenic response after transplantation. Recently we reported on the potential of the decell...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/3/035023
更新日期:2014-09-01 00:00:00
abstract::The high attrition rate of neuro-pharmaceuticals as they proceed to market necessitates the development of clinically-relevant in vitro neural microphysiological systems that can be utilized during the preclinical screening phase to assess the safety and efficacy of potential compounds. Historically, proposed models h...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab41b4
更新日期:2019-10-21 00:00:00
abstract::We demonstrate a novel application of TiO2 photocatalysis for modifying the cell affinity of a scaffold surface in a cell-culture environment. An as-deposited octadecyltrichlorosilane self-assembled monolayer (OTS SAM) on TiO2 was found to be hydrophobic and stably adsorbed serum albumins that blocked subsequent adsor...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/3/035021
更新日期:2014-09-01 00:00:00
abstract::A Caco-2 cell culture on Transwell, an alternative testing to animal or human testing used in evaluating drug intestinal permeability, incorrectly estimated the absorption of actively transported drugs due to the low expression of membrane transporters. Similarly, three-dimensional (3D) cultures of Caco-2 cells, which...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/7/1/015003
更新日期:2014-12-17 00:00:00
abstract::Tissue rings with incorporated microscaffolds have been engineered as promising building blocks for constructing biological tubes from the bottom up. However, the microscaffolds available for incorporation are very limited at present. In this paper we provide an efficient strategy to first incorporate microfluidic spu...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab1ee5
更新日期:2019-06-25 00:00:00
abstract::The physical and mechanical properties of cells modulate their behavior such proliferation rate, migration and extracellular matrix remodeling. In order to study cell behavior in a tissue-like environment in vitro, it is of utmost importance to develop biologically and physically relevant 3D cell models. Here, we char...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa6ed5
更新日期:2017-05-11 00:00:00
abstract::One of the major issues in tissue engineering has been the development of three-dimensional (3D) scaffolds, which serve as a structural template for cell growth and extracellular matrix formation. In scaffold-based tissue engineering, 3D printing (3DP) technology has been successfully applied for the fabrication of co...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/2/025003
更新日期:2014-06-01 00:00:00
abstract::Three-dimensional bioprinting of biomaterials shows great potential for producing cell-encapsulated scaffolds to repair nerves after injury or disease. For this, preparation of biomaterials and bioprinting itself are critical to create scaffolds with both biological and mechanical properties appropriate for nerve rege...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aacd30
更新日期:2018-06-29 00:00:00
abstract::Four-dimensional (4D) bioprinting of cell-laden constructs with programmable shape-morphing structures has gained increasing attention in the field of biofabrication and tissue engineering. Currently, most of the widely used materials for 4D printing, including N-isopropylacrylamide-based polymers, are not commonly us...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab39c5
更新日期:2019-09-13 00:00:00
abstract::Electrospinning has been widely used to produce ultrafine fibers in microscale and nanoscale; however, traditional electrospinning processes are currently beset by troublesome limitations in fabrication of 3D periodic porous structures because of the chaotic nature of the electrospinning jet. Here we report a novel st...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/7/4/045004
更新日期:2015-11-05 00:00:00
abstract::The inkjet ejection technology used in printers has been adopted and research has been conducted on manufacturing artificial tissue by patterning cells through micronozzle ejection of small droplets containing multiple cells. However, stable injection of cells has proven difficult, owing to the frequent occurrence of ...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/4/4/045005
更新日期:2012-12-01 00:00:00
abstract::The aim of this study was to comparatively evaluate the angiogenic capacity of cocultures using either human bone marrow- or human adipose tissue-derived mesenchymal stem cells (MSCs) (BM- or AT-MSCs) with human umbilical vein endothelial cells (HUVECs) both in vitro and in vivo at early time points (i.e. days 3 and 7...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/1/015005
更新日期:2014-03-01 00:00:00
abstract::Four-dimensional (4D) bioprinting, encompassing a wide range of disciplines including bioengineering, materials science, chemistry, and computer sciences, is emerging as the next-generation biofabrication technology. By utilizing stimuli-responsive materials and advanced three-dimensional (3D) bioprinting strategies, ...
journal_title:Biofabrication
pub_type: 杂志文章,评审
doi:10.1088/1758-5090/9/1/012001
更新日期:2016-12-02 00:00:00
abstract::In clinical islet transplantation, allogeneic islets of Langerhans are transplanted into the portal vein of patients with type 1 diabetes, enabling the restoration of normoglycemia. After intra-hepatic transplantation several factors are involved in the decay in islet mass and function mainly caused by an immediate bl...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/7/2/025009
更新日期:2015-05-28 00:00:00
abstract::In conventional primary cultures, cardiac cells prepared from a newborn rat undergo spontaneous formation of cell clusters after several days. These cell clusters may be non-homogeneously distributed on a flat surface and show irregular beating which can be recorded by calcium ion imaging. In order to improve the cell...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/5/3/035013
更新日期:2013-09-01 00:00:00
abstract::Silica-bonded calcite scaffolds have been successfully 3D-printed by direct ink writing, starting from a paste comprising a silicone polymer and calcite powders, calibrated in order to match a SiO2/CaCO3 weight balance of 35/65. The scaffolds, fabricated with two slightly different geometries, were first cross-linked ...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa6c37
更新日期:2017-05-11 00:00:00
abstract::Cell therapy represents a promising option for revascularization of ischemic tissues. However, injection of dispersed cells is not optimal to ensure precise homing into the recipient's vasculature. Implantation of cell-engineered scaffolds around the occluded artery may obviate these limitations. Here, we employed the...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/8/1/015020
更新日期:2016-03-24 00:00:00
abstract::A major limitation of existing 3D implantable structures for bone tissue engineering is that most of the cells rapidly attach on the outer edges of the structure, restricting the cells penetration into the inner parts and causing the formation of a necrotic core. Furthermore, these structures generally possess a rando...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aaa718
更新日期:2018-02-05 00:00:00
abstract::The precise positioning and arrangement of cell spheroids and organoids are critical to reconstructing complex tissue architecture for tissue engineering and regenerative medicine. Here, we present a digital acoustofluidic method to manipulate cell spheroids and organoids with unprecedented dexterity. By introducing l...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab9582
更新日期:2020-07-01 00:00:00
abstract::An engineered three-dimensional scaffold with hierarchical porosity and multiple niche microenvironments is produced using a combined multi-nozzle deposition-freeze casting technique. In this paper we present a process to fabricate a scaffold with improved interconnectivity and hierarchical porosity. The scaffold is p...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/1/015007
更新日期:2014-03-01 00:00:00
abstract::Cardiovascular diseases (CVDs) are considered the principal cause of worldwide death, being atherosclerosis the main etiology. Up to now, the predominant treatment for CVDs has been bypass surgery from autologous source. However, due to previous harvest or the type of disease, this is not always an option. For this re...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/abbd27
更新日期:2020-09-30 00:00:00
abstract::Advances in three-dimensional cell cultures offer new opportunities in biomedical research and drug development. However, there are still challenges to overcome, including the lack of reliability, repeatability and complexity of tissues obtained by these techniques. In this study, we describe a new bioprinting system ...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aaf625
更新日期:2018-12-27 00:00:00
abstract::Tissue engineering based on building blocks is an emerging method to fabricate 3D tissue constructs. This method requires depositing and assembling building blocks (cell-laden microgels) at high throughput. The current technologies (e.g., molding and photolithography) to fabricate microgels have throughput challenges ...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/2/1/014105
更新日期:2010-03-01 00:00:00
abstract::We demonstrate a simple, accurate and versatile method to manipulate Parylene C, a material widely known for its high biocompatibility, and transform it to a substrate that can effectively control the cellular microenvironment and consequently affect the morphology and function of the cells in vitro. The Parylene C sc...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/2/025004
更新日期:2014-06-01 00:00:00