Abstract:
:Laser-assisted bioprinting (LaBP) allows the realization of computer-generated 3D tissue grafts consisting of cells embedded in a hydrogel environment. In this study, human adipose-derived stem cells (hASCs) were printed in a free-scalable 3D grid pattern by means of LaBP. We demonstrate that neither the proliferation ability nor the differentiation behaviour of the stem cells was affected by the LaBP procedure. Furthermore, the 3D grafts were differentiated down the adipogenic lineage pathway for 10 days. We verify by quantitative assessments of adipogenic markers that the 3D grafts resemble cell lineages present in natural adipose tissue. Additionally, we provide the proof that even pre-differentiated hASCs could be utilized for the generation of 3D tissue grafts. These results indicate that the biofabrication of living grafts resembling their complex native origin is within reach.
journal_name
Biofabricationjournal_title
Biofabricationauthors
Gruene M,Pflaum M,Deiwick A,Koch L,Schlie S,Unger C,Wilhelmi M,Haverich A,Chichkov BNdoi
10.1088/1758-5082/3/1/015005subject
Has Abstractpub_date
2011-03-01 00:00:00pages
015005issue
1eissn
1758-5082issn
1758-5090pii
S1758-5082(11)77496-6journal_volume
3pub_type
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