Adipogenic differentiation of laser-printed 3D tissue grafts consisting of human adipose-derived stem cells.

Abstract:

:Laser-assisted bioprinting (LaBP) allows the realization of computer-generated 3D tissue grafts consisting of cells embedded in a hydrogel environment. In this study, human adipose-derived stem cells (hASCs) were printed in a free-scalable 3D grid pattern by means of LaBP. We demonstrate that neither the proliferation ability nor the differentiation behaviour of the stem cells was affected by the LaBP procedure. Furthermore, the 3D grafts were differentiated down the adipogenic lineage pathway for 10 days. We verify by quantitative assessments of adipogenic markers that the 3D grafts resemble cell lineages present in natural adipose tissue. Additionally, we provide the proof that even pre-differentiated hASCs could be utilized for the generation of 3D tissue grafts. These results indicate that the biofabrication of living grafts resembling their complex native origin is within reach.

journal_name

Biofabrication

journal_title

Biofabrication

authors

Gruene M,Pflaum M,Deiwick A,Koch L,Schlie S,Unger C,Wilhelmi M,Haverich A,Chichkov BN

doi

10.1088/1758-5082/3/1/015005

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

015005

issue

1

eissn

1758-5082

issn

1758-5090

pii

S1758-5082(11)77496-6

journal_volume

3

pub_type

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