Trapping cell spheroids and organoids using digital acoustofluidics.

abstract：:Recent research has been focusing on the generation of living personalized osteochondral constructs for joint repair. Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts. Therefore, we investigated the ...

journal_title：Biofabrication

authors： Mancini IAD,Schmidt S,Brommer H,Pouran B,Schäfer S,Tessmar J,Mensinga A,van Rijen MHP,Groll J,Blunk T,Levato R,Malda J,van Weeren PR

更新日期：2020-07-01 00:00:00

abstract：:Cell mechanical microenvironment (CMM) significantly affects cell behaviors such as spreading, migration, proliferation and differentiation. However, most studies on cell response to mechanical stimulation are based on two-dimensional (2D) planar substrates, which cannot mimic native three-dimensional (3D) CMM. Accumu...

journal_title：Biofabrication

authors： Huang G,Wang L,Wang S,Han Y,Wu J,Zhang Q,Xu F,Lu TJ

更新日期：2012-12-01 00:00:00

abstract：:We report a method for preparing cell-laden hydrogel tubes. This method uses a coaxial double-orifice spinneret, simpler than triple-orifice spinnerets which have been used for preparing similar constructs. The intended application was to create a template for preparing filament-like structures composed of two heterog...

journal_title：Biofabrication

authors： Sakai S,Liu Y,Mah EJ,Taya M

更新日期：2013-03-01 00:00:00

abstract：:Tissue engineering has been developed with the ultimate aim of manufacturing human organs, but success has been limited to only thin tissues and tissues with no significant structures. In order to construct more complicated tissues, we have developed a three-dimensional (3D) fabrication technology in which 3D structur...

journal_title：Biofabrication

authors： Arai K,Iwanaga S,Toda H,Genci C,Nishiyama Y,Nakamura M

更新日期：2011-09-01 00:00:00

abstract：:Four-dimensional (4D) bioprinting of cell-laden constructs with programmable shape-morphing structures has gained increasing attention in the field of biofabrication and tissue engineering. Currently, most of the widely used materials for 4D printing, including N-isopropylacrylamide-based polymers, are not commonly us...

journal_title：Biofabrication

authors： Luo Y,Lin X,Chen B,Wei X

更新日期：2019-09-13 00:00:00

abstract：:Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated. Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers whic...

journal_title：Biofabrication

authors： Chen S,Wu C,Liu A,Wei D,Xiao Y,Guo Z,Chen L,Zhu Y,Sun J,Luo H,Fan H

更新日期：2020-05-12 00:00:00

abstract：:Here we developed a composite scaffold of pearl/poly(lactic-co-glycolic acid) (pearl/PLGA) utilizing the low-temperature deposition manufacturing (LDM). LDM makes it possible to fabricate scaffolds with designed microstructure and macrostructure, while keeping the bioactivity of biomaterials by working at a low temper...

journal_title：Biofabrication

authors： Xu M,Li Y,Suo H,Yan Y,Liu L,Wang Q,Ge Y,Xu Y

更新日期：2010-06-01 00:00:00

abstract：:Laser-assisted bioprinting (LaBP) allows the realization of computer-generated 3D tissue grafts consisting of cells embedded in a hydrogel environment. In this study, human adipose-derived stem cells (hASCs) were printed in a free-scalable 3D grid pattern by means of LaBP. We demonstrate that neither the proliferation...

journal_title：Biofabrication

authors： Gruene M,Pflaum M,Deiwick A,Koch L,Schlie S,Unger C,Wilhelmi M,Haverich A,Chichkov BN

更新日期：2011-03-01 00:00:00

abstract：:Omentum-based matrices fabricated by decellularization have the potential to serve as autologous scaffolds for tissue engineering. Transplantation of such scaffolds prepared from the patient's own biomaterial may reduce the immunogenic response after transplantation. Recently we reported on the potential of the decell...

journal_title：Biofabrication

authors： Soffer-Tsur N,Shevach M,Shapira A,Peer D,Dvir T

更新日期：2014-09-01 00:00:00

abstract：:Three-dimensional bioprinting of biomaterials shows great potential for producing cell-encapsulated scaffolds to repair nerves after injury or disease. For this, preparation of biomaterials and bioprinting itself are critical to create scaffolds with both biological and mechanical properties appropriate for nerve rege...

journal_title：Biofabrication

authors： Ning L,Sun H,Lelong T,Guilloteau R,Zhu N,Schreyer DJ,Chen X

更新日期：2018-06-29 00:00:00

abstract：:Tissue engineering based on building blocks is an emerging method to fabricate 3D tissue constructs. This method requires depositing and assembling building blocks (cell-laden microgels) at high throughput. The current technologies (e.g., molding and photolithography) to fabricate microgels have throughput challenges ...

journal_title：Biofabrication

authors： Xu F,Moon SJ,Emre AE,Turali ES,Song YS,Hacking SA,Nagatomi J,Demirci U

更新日期：2010-03-01 00:00:00

abstract：:Despite the widespread use as platforms for various biomedical applications, engineering hydrogels to impart multifunctionality and control physical properties, while closely mimicking the native cellular microenvironment, is still a significant challenge. Herein, nanofibers consisting of hydrophilic and photocrosslin...

journal_title：Biofabrication

authors： Kim S,Cha C

更新日期：2019-12-19 00:00:00

abstract：:In the pharmaceutical industry, new drugs are tested to find appropriate compounds for therapeutic purposes for contemporary diseases. Unfortunately, novel compounds emerge at expensive prices and current target evaluation processes have limited throughput, thus leading to an increase of cost and time for drug develop...

journal_title：Biofabrication

authors： Rodríguez-Dévora JI,Zhang B,Reyna D,Shi ZD,Xu T

更新日期：2012-09-01 00:00:00

abstract：:Current technologies for manufacturing of microfluidic devices include soft-lithography, wet and dry etching, thermoforming, micro-machining and three-dimensional (3D) printing. Among them, soft-lithography has been the mostly preferred one in medical and pharmaceutical fields due to its ability to generate polydimeth...

journal_title：Biofabrication

authors： Ozbolat V,Dey M,Ayan B,Ozbolat IT

更新日期：2019-04-16 00:00:00

abstract：:This paper describes a multi-material virtual prototyping (MMVP) system for modelling and digital fabrication of discrete and functionally graded multi-material objects for biomedical applications. The MMVP system consists of a DMMVP module, an FGMVP module and a virtual reality (VR) simulation module. The DMMVP modul...

journal_title：Biofabrication

authors： Cheung HH,Choi SH

更新日期：2009-12-01 00:00:00

abstract：:This paper presents a novel continuous tool-path planning methodology for hollowed scaffold fabrication in tissue engineering. A new functionally gradient porous architecture is proposed with a continuous material deposition planning scheme. A controllable variational pore size and hence the porosity have been achieve...

journal_title：Biofabrication

authors： Khoda AK,Ozbolat IT,Koc B

更新日期：2011-09-01 00:00:00

abstract：:Four-dimensional (4D) bioprinting, encompassing a wide range of disciplines including bioengineering, materials science, chemistry, and computer sciences, is emerging as the next-generation biofabrication technology. By utilizing stimuli-responsive materials and advanced three-dimensional (3D) bioprinting strategies, ...

journal_title：Biofabrication

authors： Li YC,Zhang YS,Akpek A,Shin SR,Khademhosseini A

更新日期：2016-12-02 00:00:00

abstract：:Scaffold-free 3D cell cultures (e.g. pellet cultures) are widely used in medical science, including cartilage regeneration. Their drawbacks are high time/reagent consumption and lack of early readout parameters. While optimisation was achieved by automation or simplified spheroid generation, most culture systems remai...

journal_title：Biofabrication

authors： Fürsatz M,Gerges P,Wolbank S,Nürnberger S

更新日期：2021-01-29 00:00:00

abstract：:An ordered cellulose film scaffold, termed a nematic ordered cellulose (NOC) template, had unique surface properties and successfully induced the establishment of a three-dimensional (3D), hierarchical structure of epidermal cells by cell attachment and subsequent culture. Initially, the scaffold surface properties we...

journal_title：Biofabrication

authors： Seyama T,Suh EY,Kondo T

更新日期：2013-06-01 00:00:00

abstract：:Silica-bonded calcite scaffolds have been successfully 3D-printed by direct ink writing, starting from a paste comprising a silicone polymer and calcite powders, calibrated in order to match a SiO2/CaCO3 weight balance of 35/65. The scaffolds, fabricated with two slightly different geometries, were first cross-linked ...

journal_title：Biofabrication

authors： Fiocco L,Elsayed H,Badocco D,Pastore P,Bellucci D,Cannillo V,Detsch R,Boccaccini AR,Bernardo E

更新日期：2017-05-11 00:00:00

abstract：:We previously developed the Bio-Pick, Place, and Perfuse (Bio-P3) instrument to fabricate large perfusable tissue constructs by stacking and aligning scaffold-free living microtissues with integrated lumens. The Bio-P3 required an actuating mechanism to manipulate living microtissues of various sizes and shapes that a...

journal_title：Biofabrication

authors： Ip BC,Cui F,Tripathi A,Morgan JR

更新日期：2016-05-25 00:00:00

abstract：:A major limitation of existing 3D implantable structures for bone tissue engineering is that most of the cells rapidly attach on the outer edges of the structure, restricting the cells penetration into the inner parts and causing the formation of a necrotic core. Furthermore, these structures generally possess a rando...

journal_title：Biofabrication

authors： Paun IA,Popescu RC,Mustaciosu CC,Zamfirescu M,Calin BS,Mihailescu M,Dinescu M,Popescu A,Chioibasu D,Soproniy M,Luculescu CR

更新日期：2018-02-05 00:00:00

abstract：:Critical understanding of the complex metastatic cascade of prostate cancer is necessary for the development of a therapeutic interventions for treating metastatic prostate cancer. Increasing evidence supports the synergistic role of biochemical and biophysical cues in cancer progression at metastases. The biochemical...

journal_title：Biofabrication

authors： Jasuja H,Kar S,Katti DR,Katti K

更新日期：2021-01-08 00:00:00

abstract：:Calcium alginate hydrogels are widely used as biocompatible materials in a substantial number of biomedical applications. This paper reports on a hybrid 3D printing and electrodeposition approach for forming 3D calcium alginate hydrogels in a controllable manner. Firstly, a specific 3D hydrogel printing system is deve...

journal_title：Biofabrication

authors： Shang W,Liu Y,Wan W,Hu C,Liu Z,Wong CT,Fukuda T,Shen Y

更新日期：2017-06-07 00:00:00

abstract：:An important unsolved challenge in tissue engineering has been the inability to replicate the geometry and function of vascular networks and blood vessels. Here, we engineer a user-defined 3D microfluidic vascular channel using 3D printing-enabled hydrogel casting. First, a hollow L-shaped channel is developed using a...

journal_title：Biofabrication

authors： Yang L,Shridhar SV,Gerwitz M,Soman P

更新日期：2016-08-26 00:00:00

abstract：:Three-dimensional (3D) tissue models are invaluable tools that can closely reflect the in vivo physiological environment. However, they are usually difficult to develop, have a low throughput and are often costly; limiting their utility to most laboratories. The recent availability of inexpensive additive manufacturin...

journal_title：Biofabrication

authors： Flood P,Alvarez L,Reynaud EG

更新日期：2016-10-11 00:00:00

abstract：:A Caco-2 cell culture on Transwell, an alternative testing to animal or human testing used in evaluating drug intestinal permeability, incorrectly estimated the absorption of actively transported drugs due to the low expression of membrane transporters. Similarly, three-dimensional (3D) cultures of Caco-2 cells, which...

journal_title：Biofabrication

authors： Shen C,Meng Q,Zhang G

更新日期：2014-12-17 00:00:00

abstract：:Plant cell cultures produce active agents for pharmaceuticals, food and cosmetics. However, up to now process control for plant cell suspension cultures is challenging. A positive impact of cell immobilization, such as encapsulation in hydrogel beads, on secondary metabolites production has been reported for several p...

journal_title：Biofabrication

authors： Seidel J,Ahlfeld T,Adolph M,Kümmritz S,Steingroewer J,Krujatz F,Bley T,Gelinsky M,Lode A

更新日期：2017-11-14 00:00:00

abstract：:In recent years, novel biofabrication technologies have enabled the rapid manufacture of hydrogel-cell suspensions into tissue-imitating constructs. The development of novel materials for biofabrication still remains a challenge due to a gap between contradicting requirements such as three-dimensional printability and...

journal_title：Biofabrication

authors： Köpf M,Campos DF,Blaeser A,Sen KS,Fischer H

更新日期：2016-05-20 00:00:00

abstract：:Tissue engineering aims to create implantable biomaterials for the repair and regeneration of damaged tissues. In vitro tissue engineering is generally based on static culture, which limits access to nutrients and lacks mechanical signaling. Using shear stress is controversial because in some cases it can lead to cell...

journal_title：Biofabrication

authors： Salinas EY,Aryaei A,Paschos N,Berson E,Kwon H,Hu JC,Athanasiou KA

更新日期：2020-08-10 00:00:00