Cell-printed 3D liver-on-a-chip possessing a liver microenvironment and biliary system.

abstract：:In the body, cells are surrounded by an interconnected mesh of insoluble, bioactive protein fibres to which they adhere in a well-controlled manner, embedded in a hydrogel-like highly hydrated matrix. True morphological and biochemical mimicry of this so-called extracellular matrix (ECM) remains a challenge but appear...

journal_title：Biofabrication

authors： Schulte VA,Hahn K,Dhanasingh A,Heffels KH,Groll J

更新日期：2014-06-01 00:00:00

abstract：:Traditional drug discovery involves the screening of lead compounds from a chemical library by using cell-based high throughput screening (HTS) procedures. This has created a demand for the development of cell-based microarray chips for HTS of compounds. Although several cell-based microarray devices and procedures fo...

journal_title：Biofabrication

authors： Fujita S,Onuki-Nagasaki R,Ikuta K,Hara Y

更新日期：2016-12-05 00:00:00

abstract：:Four-dimensional (4D) bioprinting of cell-laden constructs with programmable shape-morphing structures has gained increasing attention in the field of biofabrication and tissue engineering. Currently, most of the widely used materials for 4D printing, including N-isopropylacrylamide-based polymers, are not commonly us...

journal_title：Biofabrication

authors： Luo Y,Lin X,Chen B,Wei X

更新日期：2019-09-13 00:00:00

abstract：:We report a method for preparing cell-laden hydrogel tubes. This method uses a coaxial double-orifice spinneret, simpler than triple-orifice spinnerets which have been used for preparing similar constructs. The intended application was to create a template for preparing filament-like structures composed of two heterog...

journal_title：Biofabrication

authors： Sakai S,Liu Y,Mah EJ,Taya M

更新日期：2013-03-01 00:00:00

abstract：:An engineered three-dimensional scaffold with hierarchical porosity and multiple niche microenvironments is produced using a combined multi-nozzle deposition-freeze casting technique. In this paper we present a process to fabricate a scaffold with improved interconnectivity and hierarchical porosity. The scaffold is p...

journal_title：Biofabrication

authors： Snyder JE,Hunger PM,Wang C,Hamid Q,Wegst UG,Sun W

更新日期：2014-03-01 00:00:00

abstract：:We report on the fabrication of silicon-reinforced carbon (C:Si) structures by combinatorial pulsed laser deposition to search for the best design for a new generation of multi-functional coated implants. The synthesized films were characterized from the morphological, structural, compositional, mechanical and microbi...

journal_title：Biofabrication

authors： Mihailescu IN,Bociaga D,Popescu-Pelin G,Stan GE,Duta L,Socol G,Chifiriuc MC,Bleotu C,Lazar V,Husanu MA,Zgura I,Miculescu F,Negut I,Hapenciuc C

更新日期：2017-06-01 00:00:00

abstract：:The tumour microenvironment is heterogeneous and consists of multiple cell types, variable extracellular matrix (ECM) composition, and contains cell-defined gradients of small molecules, oxygen, nutrients and waste. Emerging in vitro cell culture systems that attempt to replicate these features often fail to incorpora...

journal_title：Biofabrication

authors： Rodenhizer D,Cojocari D,Wouters BG,McGuigan AP

更新日期：2016-10-18 00:00:00

abstract：:An ordered cellulose film scaffold, termed a nematic ordered cellulose (NOC) template, had unique surface properties and successfully induced the establishment of a three-dimensional (3D), hierarchical structure of epidermal cells by cell attachment and subsequent culture. Initially, the scaffold surface properties we...

journal_title：Biofabrication

authors： Seyama T,Suh EY,Kondo T

更新日期：2013-06-01 00:00:00

abstract：:Tissue engineering based on building blocks is an emerging method to fabricate 3D tissue constructs. This method requires depositing and assembling building blocks (cell-laden microgels) at high throughput. The current technologies (e.g., molding and photolithography) to fabricate microgels have throughput challenges ...

journal_title：Biofabrication

authors： Xu F,Moon SJ,Emre AE,Turali ES,Song YS,Hacking SA,Nagatomi J,Demirci U

更新日期：2010-03-01 00:00:00

abstract：:We demonstrate a simple, accurate and versatile method to manipulate Parylene C, a material widely known for its high biocompatibility, and transform it to a substrate that can effectively control the cellular microenvironment and consequently affect the morphology and function of the cells in vitro. The Parylene C sc...

journal_title：Biofabrication

authors： Trantidou T,Rao C,Barrett H,Camelliti P,Pinto K,Yacoub MH,Athanasiou T,Toumazou C,Terracciano CM,Prodromakis T

更新日期：2014-06-01 00:00:00

abstract：:In this study, we report the step-gradient nanocomposite (NC) hydrogel generated easily by spatial connection of different nanocomposite hydrogel pastes varying in the concentrations of nanomaterials with the aid of a 3D printing technique. The prepared 3D printed gradient NC hydrogel has self-adhesive properties and ...

journal_title：Biofabrication

authors： Motealleh A,Çelebi-Saltik B,Ermis N,Nowak S,Khademhosseini A,Kehr NS

更新日期：2019-08-22 00:00:00

abstract：:Plant cell cultures produce active agents for pharmaceuticals, food and cosmetics. However, up to now process control for plant cell suspension cultures is challenging. A positive impact of cell immobilization, such as encapsulation in hydrogel beads, on secondary metabolites production has been reported for several p...

journal_title：Biofabrication

authors： Seidel J,Ahlfeld T,Adolph M,Kümmritz S,Steingroewer J,Krujatz F,Bley T,Gelinsky M,Lode A

更新日期：2017-11-14 00:00:00

abstract：:Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated. Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers whic...

journal_title：Biofabrication

authors： Chen S,Wu C,Liu A,Wei D,Xiao Y,Guo Z,Chen L,Zhu Y,Sun J,Luo H,Fan H

更新日期：2020-05-12 00:00:00

abstract：:A bio-inspired hydrogel for 3D bioprinting of soft free-standing neural tissues is presented. The novel filler-free bioinks were designed by combining natural polymers for extracellular matrix biomimicry with synthetic polymers to endow desirable rheological properties for 3D bioprinting. Crosslinking of thiolated Plu...

journal_title：Biofabrication

authors： Haring AP,Thompson EG,Tong Y,Laheri S,Cesewski E,Sontheimer H,Johnson BN

更新日期：2019-02-25 00:00:00

abstract：:Scaffold-free 3D cell cultures (e.g. pellet cultures) are widely used in medical science, including cartilage regeneration. Their drawbacks are high time/reagent consumption and lack of early readout parameters. While optimisation was achieved by automation or simplified spheroid generation, most culture systems remai...

journal_title：Biofabrication

authors： Fürsatz M,Gerges P,Wolbank S,Nürnberger S

更新日期：2021-01-29 00:00:00

abstract：:We have developed cell sheet technology as a modern method for the fabrication of functional tissue-like and organ-like structures. This technology allows for a sheet of interconnected cells and cells in full contact with their natural extracellular environment to be obtained. A cell sheet can be patterned and compose...

journal_title：Biofabrication

authors： Hannachi IE,Yamato M,Okano T

更新日期：2009-06-01 00:00:00

abstract：:Native small intestine possesses distinct multi-scale structures (e.g., crypts, villi) not included in traditional 2D intestinal culture models for drug delivery and regenerative medicine. The known impact of structure on cell function motivates exploration of the influence of intestinal topography on the phenotype of...

journal_title：Biofabrication

authors： Koppes AN,Kamath M,Pfluger CA,Burkey DD,Dokmeci M,Wang L,Carrier RL

更新日期：2016-08-22 00:00:00

abstract：:Cardiovascular diseases (CVDs) are considered the principal cause of worldwide death, being atherosclerosis the main etiology. Up to now, the predominant treatment for CVDs has been bypass surgery from autologous source. However, due to previous harvest or the type of disease, this is not always an option. For this re...

journal_title：Biofabrication

authors： Bosch Rué E,Delgado LM,Gil FJ,Perez RA

更新日期：2020-09-30 00:00:00

abstract：:The precise positioning and arrangement of cell spheroids and organoids are critical to reconstructing complex tissue architecture for tissue engineering and regenerative medicine. Here, we present a digital acoustofluidic method to manipulate cell spheroids and organoids with unprecedented dexterity. By introducing l...

journal_title：Biofabrication

authors： Cai H,Wu Z,Ao Z,Nunez A,Chen B,Jiang L,Bondesson M,Guo F

更新日期：2020-07-01 00:00:00

abstract：:Tissue engineering has been developed with the ultimate aim of manufacturing human organs, but success has been limited to only thin tissues and tissues with no significant structures. In order to construct more complicated tissues, we have developed a three-dimensional (3D) fabrication technology in which 3D structur...

journal_title：Biofabrication

authors： Arai K,Iwanaga S,Toda H,Genci C,Nishiyama Y,Nakamura M

更新日期：2011-09-01 00:00:00

abstract：:Current technologies for manufacturing of microfluidic devices include soft-lithography, wet and dry etching, thermoforming, micro-machining and three-dimensional (3D) printing. Among them, soft-lithography has been the mostly preferred one in medical and pharmaceutical fields due to its ability to generate polydimeth...

journal_title：Biofabrication

authors： Ozbolat V,Dey M,Ayan B,Ozbolat IT

更新日期：2019-04-16 00:00:00

abstract：:We demonstrate a novel application of TiO2 photocatalysis for modifying the cell affinity of a scaffold surface in a cell-culture environment. An as-deposited octadecyltrichlorosilane self-assembled monolayer (OTS SAM) on TiO2 was found to be hydrophobic and stably adsorbed serum albumins that blocked subsequent adsor...

journal_title：Biofabrication

authors： Yamamoto H,Demura T,Morita M,Kono S,Sekine K,Shinada T,Nakamura S,Tanii T

更新日期：2014-09-01 00:00:00

abstract：:In conventional primary cultures, cardiac cells prepared from a newborn rat undergo spontaneous formation of cell clusters after several days. These cell clusters may be non-homogeneously distributed on a flat surface and show irregular beating which can be recorded by calcium ion imaging. In order to improve the cell...

journal_title：Biofabrication

authors： Wang L,Liu L,Magome N,Agladze K,Chen Y

更新日期：2013-09-01 00:00:00

abstract：:In this paper we explore the printability of reversible networks formed by catechol functionalized PEG solutions and metal cations (Al3+, Fe3+ or V3+). The printability and shape fidelity were dependent on the ink composition (metal ion type, pH, PEG molecular weight) and printing parameters (extrusion pressure and pr...

journal_title：Biofabrication

authors： Włodarczyk-Biegun MK,Paez JI,Villiou M,Feng J,Del Campo A

更新日期：2020-04-29 00:00:00

abstract：:Electrospun fibers of natural polymers are desirable for biomedical applications such as tissue engineering. Crosslinking of electrospun fibers of natural polymers is needed to prevent dissolution in water and to enhance mechanical strength. In this study, an in situ UV-crosslinking method was developed for crosslinki...

journal_title：Biofabrication

authors： Lin WH,Tsai WB

更新日期：2013-09-01 00:00:00

abstract：:Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different p...

journal_title：Biofabrication

authors： Hendrikson WJ,Rouwkema J,Clementi F,van Blitterswijk CA,Farè S,Moroni L

更新日期：2017-08-02 00:00:00

abstract：:Degeneration of the intervertebral disc (IVD) represents a significant musculoskeletal disease burden. Tissue engineering has proposed several strategies comprising the use of biodegradable materials to prepare scaffolds that can present mechanical properties similar to those of native IVD tissues. However, this might...

journal_title：Biofabrication

authors： van Uden S,Silva-Correia J,Correlo VM,Oliveira JM,Reis RL

更新日期：2015-01-21 00:00:00

abstract：:An important unsolved challenge in tissue engineering has been the inability to replicate the geometry and function of vascular networks and blood vessels. Here, we engineer a user-defined 3D microfluidic vascular channel using 3D printing-enabled hydrogel casting. First, a hollow L-shaped channel is developed using a...

journal_title：Biofabrication

authors： Yang L,Shridhar SV,Gerwitz M,Soman P

更新日期：2016-08-26 00:00:00

abstract：:Human induced pluripotent stem cells (hiPSCs) can be differentiated at high efficiency into cells of a targeting type but the resulting cell population has to be of high purity for clinical therapies to avoid teratomas. Herein, we report a microfluidic device with integrated and surface functionalised fishnet-like str...

journal_title：Biofabrication

authors： Li X,Yu L,Li J,Minami I,Nakajima M,Noda Y,Kotera H,Liu L,Chen Y

更新日期：2016-09-08 00:00:00

abstract：:How to pack materials into well-defined volumes efficiently has been a longstanding question of interest to physicists, material scientists, and mathematicians as these materials have broad applications ranging from shipping goods in commerce to seeds in agriculture and to spheroids in tissue engineering. How many mar...

journal_title：Biofabrication

authors： Ren T,Steiger W,Chen P,Ovsianikov A,Demirci U

更新日期：2020-03-31 00:00:00