Abstract:
:Advances in three-dimensional cell cultures offer new opportunities in biomedical research and drug development. However, there are still challenges to overcome, including the lack of reliability, repeatability and complexity of tissues obtained by these techniques. In this study, we describe a new bioprinting system called reactive jet impingement (ReJI) for the bioprinting of cell-laden hydrogels. Droplets of gel precursor solutions are jetted at one another such that they meet and react in mid-air before the gel droplets fall to the substrate. This technique offers a combination of deposition rate, cell density and cell viability which is not currently matched by any other bioprinting technique. The importance of cell density is demonstrated in the development of bone microtissues derived from immortalised human bone marrow stem cells. The cells were printed with high viability within a collagen-alginate-fibrin gel, and tissue specific gene expression shows significantly higher tissue maturation rates using the ability of the ReJI system to deposit gels with a high cell density.
journal_name
Biofabricationjournal_title
Biofabricationauthors
da Conceicao Ribeiro R,Pal D,Ferreira AM,Gentile P,Benning M,Dalgarno Kdoi
10.1088/1758-5090/aaf625subject
Has Abstractpub_date
2018-12-27 00:00:00pages
015014issue
1eissn
1758-5082issn
1758-5090journal_volume
11pub_type
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