Direct printing of patterned three-dimensional ultrafine fibrous scaffolds by stable jet electrospinning for cellular ingrowth.


:Electrospinning has been widely used to produce ultrafine fibers in microscale and nanoscale; however, traditional electrospinning processes are currently beset by troublesome limitations in fabrication of 3D periodic porous structures because of the chaotic nature of the electrospinning jet. Here we report a novel strategy to print 3D poly(L-lactic acid) (PLLA) ultrafine fibrous scaffolds with the fiber diameter of approximately 2 μm by combining a stable jet electrospinning method and an X-Y stage technique. Our approach allows linearly deposited electrospun ultrafine fibers to assemble into 3D structures with tunable pore sizes and desired patterns. Process conditions (e.g., plotting speed, feeding rate, and collecting distance) were investigated in order to achieve stable jet printing of ultrafine PLLA fibers. The proposed 3D scaffold was successfully used for cell penetration and growth, demonstrating great potential for tissue engineering applications.






Yuan H,Zhou Q,Li B,Bao M,Lou X,Zhang Y




Has Abstract


2015-11-05 00:00:00












  • 4D bioprinting: the next-generation technology for biofabrication enabled by stimuli-responsive materials.

    abstract::Four-dimensional (4D) bioprinting, encompassing a wide range of disciplines including bioengineering, materials science, chemistry, and computer sciences, is emerging as the next-generation biofabrication technology. By utilizing stimuli-responsive materials and advanced three-dimensional (3D) bioprinting strategies, ...


    pub_type: 杂志文章,评审


    authors: Li YC,Zhang YS,Akpek A,Shin SR,Khademhosseini A

    更新日期:2016-12-02 00:00:00

  • 3D bioprinting of scaffolds with living Schwann cells for potential nerve tissue engineering applications.

    abstract::Three-dimensional bioprinting of biomaterials shows great potential for producing cell-encapsulated scaffolds to repair nerves after injury or disease. For this, preparation of biomaterials and bioprinting itself are critical to create scaffolds with both biological and mechanical properties appropriate for nerve rege...


    pub_type: 杂志文章


    authors: Ning L,Sun H,Lelong T,Guilloteau R,Zhu N,Schreyer DJ,Chen X

    更新日期:2018-06-29 00:00:00

  • Custom-tailored tissue engineered polycaprolactone scaffolds for total disc replacement.

    abstract::Degeneration of the intervertebral disc (IVD) represents a significant musculoskeletal disease burden. Tissue engineering has proposed several strategies comprising the use of biodegradable materials to prepare scaffolds that can present mechanical properties similar to those of native IVD tissues. However, this might...


    pub_type: 杂志文章


    authors: van Uden S,Silva-Correia J,Correlo VM,Oliveira JM,Reis RL

    更新日期:2015-01-21 00:00:00

  • Printability study of metal ion crosslinked PEG-catechol based inks.

    abstract::In this paper we explore the printability of reversible networks formed by catechol functionalized PEG solutions and metal cations (Al3+, Fe3+ or V3+). The printability and shape fidelity were dependent on the ink composition (metal ion type, pH, PEG molecular weight) and printing parameters (extrusion pressure and pr...


    pub_type: 杂志文章


    authors: Włodarczyk-Biegun MK,Paez JI,Villiou M,Feng J,Del Campo A

    更新日期:2020-04-29 00:00:00

  • Optimized silicon reinforcement of carbon coatings by pulsed laser technique for superior functional biomedical surfaces fabrication.

    abstract::We report on the fabrication of silicon-reinforced carbon (C:Si) structures by combinatorial pulsed laser deposition to search for the best design for a new generation of multi-functional coated implants. The synthesized films were characterized from the morphological, structural, compositional, mechanical and microbi...


    pub_type: 杂志文章


    authors: Mihailescu IN,Bociaga D,Popescu-Pelin G,Stan GE,Duta L,Socol G,Chifiriuc MC,Bleotu C,Lazar V,Husanu MA,Zgura I,Miculescu F,Negut I,Hapenciuc C

    更新日期:2017-06-01 00:00:00

  • Fabricating a pearl/PLGA composite scaffold by the low-temperature deposition manufacturing technique for bone tissue engineering.

    abstract::Here we developed a composite scaffold of pearl/poly(lactic-co-glycolic acid) (pearl/PLGA) utilizing the low-temperature deposition manufacturing (LDM). LDM makes it possible to fabricate scaffolds with designed microstructure and macrostructure, while keeping the bioactivity of biomaterials by working at a low temper...


    pub_type: 杂志文章


    authors: Xu M,Li Y,Suo H,Yan Y,Liu L,Wang Q,Ge Y,Xu Y

    更新日期:2010-06-01 00:00:00

  • Development of TRACER: tissue roll for analysis of cellular environment and response.

    abstract::The tumour microenvironment is heterogeneous and consists of multiple cell types, variable extracellular matrix (ECM) composition, and contains cell-defined gradients of small molecules, oxygen, nutrients and waste. Emerging in vitro cell culture systems that attempt to replicate these features often fail to incorpora...


    pub_type: 杂志文章


    authors: Rodenhizer D,Cojocari D,Wouters BG,McGuigan AP

    更新日期:2016-10-18 00:00:00

  • Combined multi-nozzle deposition and freeze casting process to superimpose two porous networks for hierarchical three-dimensional microenvironment.

    abstract::An engineered three-dimensional scaffold with hierarchical porosity and multiple niche microenvironments is produced using a combined multi-nozzle deposition-freeze casting technique. In this paper we present a process to fabricate a scaffold with improved interconnectivity and hierarchical porosity. The scaffold is p...


    pub_type: 杂志文章


    authors: Snyder JE,Hunger PM,Wang C,Hamid Q,Wegst UG,Sun W

    更新日期:2014-03-01 00:00:00

  • Free-floating epithelial micro-tissue arrays: a low cost and versatile technique.

    abstract::Three-dimensional (3D) tissue models are invaluable tools that can closely reflect the in vivo physiological environment. However, they are usually difficult to develop, have a low throughput and are often costly; limiting their utility to most laboratories. The recent availability of inexpensive additive manufacturin...


    pub_type: 杂志文章


    authors: Flood P,Alvarez L,Reynaud EG

    更新日期:2016-10-11 00:00:00

  • Towards 4D printed scaffolds for tissue engineering: exploiting 3D shape memory polymers to deliver time-controlled stimulus on cultured cells.

    abstract::Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different p...


    pub_type: 杂志文章


    authors: Hendrikson WJ,Rouwkema J,Clementi F,van Blitterswijk CA,Farè S,Moroni L

    更新日期:2017-08-02 00:00:00

  • On chip purification of hiPSC-derived cardiomyocytes using a fishnet-like microstructure.

    abstract::Human induced pluripotent stem cells (hiPSCs) can be differentiated at high efficiency into cells of a targeting type but the resulting cell population has to be of high purity for clinical therapies to avoid teratomas. Herein, we report a microfluidic device with integrated and surface functionalised fishnet-like str...


    pub_type: 杂志文章


    authors: Li X,Yu L,Li J,Minami I,Nakajima M,Noda Y,Kotera H,Liu L,Chen Y

    更新日期:2016-09-08 00:00:00

  • Engineering three-dimensional cell mechanical microenvironment with hydrogels.

    abstract::Cell mechanical microenvironment (CMM) significantly affects cell behaviors such as spreading, migration, proliferation and differentiation. However, most studies on cell response to mechanical stimulation are based on two-dimensional (2D) planar substrates, which cannot mimic native three-dimensional (3D) CMM. Accumu...


    pub_type: 杂志文章,评审


    authors: Huang G,Wang L,Wang S,Han Y,Wu J,Zhang Q,Xu F,Lu TJ

    更新日期:2012-12-01 00:00:00

  • A functionally gradient variational porosity architecture for hollowed scaffolds fabrication.

    abstract::This paper presents a novel continuous tool-path planning methodology for hollowed scaffold fabrication in tissue engineering. A new functionally gradient porous architecture is proposed with a continuous material deposition planning scheme. A controllable variational pore size and hence the porosity have been achieve...


    pub_type: 杂志文章


    authors: Khoda AK,Ozbolat IT,Koc B

    更新日期:2011-09-01 00:00:00

  • Hybrid 3D printing and electrodeposition approach for controllable 3D alginate hydrogel formation.

    abstract::Calcium alginate hydrogels are widely used as biocompatible materials in a substantial number of biomedical applications. This paper reports on a hybrid 3D printing and electrodeposition approach for forming 3D calcium alginate hydrogels in a controllable manner. Firstly, a specific 3D hydrogel printing system is deve...


    pub_type: 杂志文章


    authors: Shang W,Liu Y,Wan W,Hu C,Liu Z,Wong CT,Fukuda T,Shen Y

    更新日期:2017-06-07 00:00:00

  • Three-dimensional tissues using human pluripotent stem cell spheroids as biofabrication building blocks.

    abstract::A recently emerged approach for tissue engineering is to biofabricate tissues using cellular spheroids as building blocks. Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), can be cultured to generate large numbers of cells and can presumably...


    pub_type: 杂志文章


    authors: Lin H,Li Q,Lei Y

    更新日期:2017-04-24 00:00:00

  • Horseradish peroxidase/catalase-mediated cell-laden alginate-based hydrogel tube production in two-phase coaxial flow of aqueous solutions for filament-like tissues fabrication.

    abstract::We report a method for preparing cell-laden hydrogel tubes. This method uses a coaxial double-orifice spinneret, simpler than triple-orifice spinnerets which have been used for preparing similar constructs. The intended application was to create a template for preparing filament-like structures composed of two heterog...


    pub_type: 杂志文章


    authors: Sakai S,Liu Y,Mah EJ,Taya M

    更新日期:2013-03-01 00:00:00

  • Cell adhesion pattern created by OSTE polymers.

    abstract::Engineering surfaces with functional polymers is a crucial issue in the field of micro/nanofabrication and cell-material interface studies. For many applications of surface patterning, it does not need cells to attach on the whole surface. Herein, we introduce a novel polymer fabrication protocol of off-stoichiometry ...


    pub_type: 杂志文章


    authors: Liu W,Li Y,Ding X

    更新日期:2017-04-24 00:00:00

  • Influence of patterned topographic features on the formation of cardiac cell clusters and their rhythmic activities.

    abstract::In conventional primary cultures, cardiac cells prepared from a newborn rat undergo spontaneous formation of cell clusters after several days. These cell clusters may be non-homogeneously distributed on a flat surface and show irregular beating which can be recorded by calcium ion imaging. In order to improve the cell...


    pub_type: 杂志文章


    authors: Wang L,Liu L,Magome N,Agladze K,Chen Y

    更新日期:2013-09-01 00:00:00

  • In situ UV-crosslinking gelatin electrospun fibers for tissue engineering applications.

    abstract::Electrospun fibers of natural polymers are desirable for biomedical applications such as tissue engineering. Crosslinking of electrospun fibers of natural polymers is needed to prevent dissolution in water and to enhance mechanical strength. In this study, an in situ UV-crosslinking method was developed for crosslinki...


    pub_type: 杂志文章


    authors: Lin WH,Tsai WB

    更新日期:2013-09-01 00:00:00

  • Optimizing the biofabrication process of omentum-based scaffolds for engineering autologous tissues.

    abstract::Omentum-based matrices fabricated by decellularization have the potential to serve as autologous scaffolds for tissue engineering. Transplantation of such scaffolds prepared from the patient's own biomaterial may reduce the immunogenic response after transplantation. Recently we reported on the potential of the decell...


    pub_type: 杂志文章


    authors: Soffer-Tsur N,Shevach M,Shapira A,Peer D,Dvir T

    更新日期:2014-09-01 00:00:00

  • A tailored three-dimensionally printable agarose-collagen blend allows encapsulation, spreading, and attachment of human umbilical artery smooth muscle cells.

    abstract::In recent years, novel biofabrication technologies have enabled the rapid manufacture of hydrogel-cell suspensions into tissue-imitating constructs. The development of novel materials for biofabrication still remains a challenge due to a gap between contradicting requirements such as three-dimensional printability and...


    pub_type: 杂志文章


    authors: Köpf M,Campos DF,Blaeser A,Sen KS,Fischer H

    更新日期:2016-05-20 00:00:00

  • A composite hydrogel-3D printed thermoplast osteochondral anchor as example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model.

    abstract::Recent research has been focusing on the generation of living personalized osteochondral constructs for joint repair. Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts. Therefore, we investigated the ...


    pub_type: 杂志文章


    authors: Mancini IAD,Schmidt S,Brommer H,Pouran B,Schäfer S,Tessmar J,Mensinga A,van Rijen MHP,Groll J,Blunk T,Levato R,Malda J,van Weeren PR

    更新日期:2020-07-01 00:00:00

  • Reactive jet impingement bioprinting of high cell density gels for bone microtissue fabrication.

    abstract::Advances in three-dimensional cell cultures offer new opportunities in biomedical research and drug development. However, there are still challenges to overcome, including the lack of reliability, repeatability and complexity of tissues obtained by these techniques. In this study, we describe a new bioprinting system ...


    pub_type: 杂志文章


    authors: da Conceicao Ribeiro R,Pal D,Ferreira AM,Gentile P,Benning M,Dalgarno K

    更新日期:2018-12-27 00:00:00

  • Biofabrication of neural microphysiological systems using magnetic spheroid bioprinting.

    abstract::The high attrition rate of neuro-pharmaceuticals as they proceed to market necessitates the development of clinically-relevant in vitro neural microphysiological systems that can be utilized during the preclinical screening phase to assess the safety and efficacy of potential compounds. Historically, proposed models h...


    pub_type: 杂志文章


    authors: Bowser DA,Moore MJ

    更新日期:2019-10-21 00:00:00

  • Fabrication of three-dimensional bioplotted hydrogel scaffolds for islets of Langerhans transplantation.

    abstract::In clinical islet transplantation, allogeneic islets of Langerhans are transplanted into the portal vein of patients with type 1 diabetes, enabling the restoration of normoglycemia. After intra-hepatic transplantation several factors are involved in the decay in islet mass and function mainly caused by an immediate bl...


    pub_type: 杂志文章


    authors: Marchioli G,van Gurp L,van Krieken PP,Stamatialis D,Engelse M,van Blitterswijk CA,Karperien MB,de Koning E,Alblas J,Moroni L,van Apeldoorn AA

    更新日期:2015-05-28 00:00:00

  • Perfusion bioreactor enabled fluid-derived shear stress conditions for novel bone metastatic prostate cancer testbed.

    abstract::Critical understanding of the complex metastatic cascade of prostate cancer is necessary for the development of a therapeutic interventions for treating metastatic prostate cancer. Increasing evidence supports the synergistic role of biochemical and biophysical cues in cancer progression at metastases. The biochemical...


    pub_type: 杂志文章


    authors: Jasuja H,Kar S,Katti DR,Katti K

    更新日期:2021-01-08 00:00:00

  • Trapping cell spheroids and organoids using digital acoustofluidics.

    abstract::The precise positioning and arrangement of cell spheroids and organoids are critical to reconstructing complex tissue architecture for tissue engineering and regenerative medicine. Here, we present a digital acoustofluidic method to manipulate cell spheroids and organoids with unprecedented dexterity. By introducing l...


    pub_type: 杂志文章


    authors: Cai H,Wu Z,Ao Z,Nunez A,Chen B,Jiang L,Bondesson M,Guo F

    更新日期:2020-07-01 00:00:00

  • Neural priming of adipose-derived stem cells by cell-imprinted substrates.

    abstract::Cell-imprinting technology is a novel method for directing stem cell fate using substrates molded from target cells. Here, we fabricated and studied cell-imprinted substrates for neural priming in human adipose-derived stem cells in the absence of chemical cues. We molded polydimethylsiloxane (PDMS) silicone substrate...


    pub_type: 杂志文章


    authors: Ghazali ZS,Eskandari M,Bonakdar S,Renaud P,Mashinchian O,Shalileh S,Bonini F,Uckay I,Preynat-Seauve O,Braschler T

    更新日期:2020-10-30 00:00:00

  • Biofabrication of nerve fibers with mimetic myelin sheath-like structure and aligned fibrous niche.

    abstract::Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated. Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers whic...


    pub_type: 杂志文章


    authors: Chen S,Wu C,Liu A,Wei D,Xiao Y,Guo Z,Chen L,Zhu Y,Sun J,Luo H,Fan H

    更新日期:2020-05-12 00:00:00

  • Three-dimensional culture of epidermal cells on ordered cellulose scaffolds.

    abstract::An ordered cellulose film scaffold, termed a nematic ordered cellulose (NOC) template, had unique surface properties and successfully induced the establishment of a three-dimensional (3D), hierarchical structure of epidermal cells by cell attachment and subsequent culture. Initially, the scaffold surface properties we...


    pub_type: 杂志文章


    authors: Seyama T,Suh EY,Kondo T

    更新日期:2013-06-01 00:00:00