Direct printing of patterned three-dimensional ultrafine fibrous scaffolds by stable jet electrospinning for cellular ingrowth.

abstract：:Fabricating three-dimensional, biocompatible microenvironments to support functional tissue assembly remains a key challenge in cardiac tissue engineering. We hypothesized that since the omentum can be removed from patients by minimally invasive procedures, the obtained underlying matrices can be manipulated to serve ...

journal_title：Biofabrication

authors： Shevach M,Soffer-Tsur N,Fleischer S,Shapira A,Dvir T

更新日期：2014-06-01 00:00:00

abstract：:Plant cell cultures produce active agents for pharmaceuticals, food and cosmetics. However, up to now process control for plant cell suspension cultures is challenging. A positive impact of cell immobilization, such as encapsulation in hydrogel beads, on secondary metabolites production has been reported for several p...

journal_title：Biofabrication

authors： Seidel J,Ahlfeld T,Adolph M,Kümmritz S,Steingroewer J,Krujatz F,Bley T,Gelinsky M,Lode A

更新日期：2017-11-14 00:00:00

abstract：:We previously developed the Bio-Pick, Place, and Perfuse (Bio-P3) instrument to fabricate large perfusable tissue constructs by stacking and aligning scaffold-free living microtissues with integrated lumens. The Bio-P3 required an actuating mechanism to manipulate living microtissues of various sizes and shapes that a...

journal_title：Biofabrication

authors： Ip BC,Cui F,Tripathi A,Morgan JR

更新日期：2016-05-25 00:00:00

abstract：:In this study, we report the step-gradient nanocomposite (NC) hydrogel generated easily by spatial connection of different nanocomposite hydrogel pastes varying in the concentrations of nanomaterials with the aid of a 3D printing technique. The prepared 3D printed gradient NC hydrogel has self-adhesive properties and ...

journal_title：Biofabrication

authors： Motealleh A,Çelebi-Saltik B,Ermis N,Nowak S,Khademhosseini A,Kehr NS

更新日期：2019-08-22 00:00:00

abstract：:An engineered three-dimensional scaffold with hierarchical porosity and multiple niche microenvironments is produced using a combined multi-nozzle deposition-freeze casting technique. In this paper we present a process to fabricate a scaffold with improved interconnectivity and hierarchical porosity. The scaffold is p...

journal_title：Biofabrication

authors： Snyder JE,Hunger PM,Wang C,Hamid Q,Wegst UG,Sun W

更新日期：2014-03-01 00:00:00

abstract：:A critical consideration in tissue engineering is to recapitulate the microstructural organization of native tissues that is essential to their function. Scaffold-based techniques have focused on achieving this via the contact guidance principle wherein topographical cues offered by scaffold fibers direct migration an...

journal_title：Biofabrication

authors： Chansoria P,Narayanan LK,Schuchard K,Shirwaiker R

更新日期：2019-04-26 00:00:00

abstract：:The precise positioning and arrangement of cell spheroids and organoids are critical to reconstructing complex tissue architecture for tissue engineering and regenerative medicine. Here, we present a digital acoustofluidic method to manipulate cell spheroids and organoids with unprecedented dexterity. By introducing l...

journal_title：Biofabrication

authors： Cai H,Wu Z,Ao Z,Nunez A,Chen B,Jiang L,Bondesson M,Guo F

更新日期：2020-07-01 00:00:00

abstract：:A recently emerged approach for tissue engineering is to biofabricate tissues using cellular spheroids as building blocks. Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), can be cultured to generate large numbers of cells and can presumably...

journal_title：Biofabrication

authors： Lin H,Li Q,Lei Y

更新日期：2017-04-24 00:00:00

abstract：:Scaffold-free 3D cell cultures (e.g. pellet cultures) are widely used in medical science, including cartilage regeneration. Their drawbacks are high time/reagent consumption and lack of early readout parameters. While optimisation was achieved by automation or simplified spheroid generation, most culture systems remai...

journal_title：Biofabrication

authors： Fürsatz M,Gerges P,Wolbank S,Nürnberger S

更新日期：2021-01-29 00:00:00

abstract：:We have developed cell sheet technology as a modern method for the fabrication of functional tissue-like and organ-like structures. This technology allows for a sheet of interconnected cells and cells in full contact with their natural extracellular environment to be obtained. A cell sheet can be patterned and compose...

journal_title：Biofabrication

authors： Hannachi IE,Yamato M,Okano T

更新日期：2009-06-01 00:00:00

abstract：:A major limitation of existing 3D implantable structures for bone tissue engineering is that most of the cells rapidly attach on the outer edges of the structure, restricting the cells penetration into the inner parts and causing the formation of a necrotic core. Furthermore, these structures generally possess a rando...

journal_title：Biofabrication

authors： Paun IA,Popescu RC,Mustaciosu CC,Zamfirescu M,Calin BS,Mihailescu M,Dinescu M,Popescu A,Chioibasu D,Soproniy M,Luculescu CR

更新日期：2018-02-05 00:00:00

abstract：:Three-dimensional bioprinting of biomaterials shows great potential for producing cell-encapsulated scaffolds to repair nerves after injury or disease. For this, preparation of biomaterials and bioprinting itself are critical to create scaffolds with both biological and mechanical properties appropriate for nerve rege...

journal_title：Biofabrication

authors： Ning L,Sun H,Lelong T,Guilloteau R,Zhu N,Schreyer DJ,Chen X

更新日期：2018-06-29 00:00:00

abstract：:In recent years, novel biofabrication technologies have enabled the rapid manufacture of hydrogel-cell suspensions into tissue-imitating constructs. The development of novel materials for biofabrication still remains a challenge due to a gap between contradicting requirements such as three-dimensional printability and...

journal_title：Biofabrication

authors： Köpf M,Campos DF,Blaeser A,Sen KS,Fischer H

更新日期：2016-05-20 00:00:00

abstract：:Despite the widespread use as platforms for various biomedical applications, engineering hydrogels to impart multifunctionality and control physical properties, while closely mimicking the native cellular microenvironment, is still a significant challenge. Herein, nanofibers consisting of hydrophilic and photocrosslin...

journal_title：Biofabrication

authors： Kim S,Cha C

更新日期：2019-12-19 00:00:00

abstract：:Omentum-based matrices fabricated by decellularization have the potential to serve as autologous scaffolds for tissue engineering. Transplantation of such scaffolds prepared from the patient's own biomaterial may reduce the immunogenic response after transplantation. Recently we reported on the potential of the decell...

journal_title：Biofabrication

authors： Soffer-Tsur N,Shevach M,Shapira A,Peer D,Dvir T

更新日期：2014-09-01 00:00:00

abstract：:The tumour microenvironment is heterogeneous and consists of multiple cell types, variable extracellular matrix (ECM) composition, and contains cell-defined gradients of small molecules, oxygen, nutrients and waste. Emerging in vitro cell culture systems that attempt to replicate these features often fail to incorpora...

journal_title：Biofabrication

authors： Rodenhizer D,Cojocari D,Wouters BG,McGuigan AP

更新日期：2016-10-18 00:00:00

abstract：:Cardiovascular diseases (CVDs) are considered the principal cause of worldwide death, being atherosclerosis the main etiology. Up to now, the predominant treatment for CVDs has been bypass surgery from autologous source. However, due to previous harvest or the type of disease, this is not always an option. For this re...

journal_title：Biofabrication

authors： Bosch Rué E,Delgado LM,Gil FJ,Perez RA

更新日期：2020-09-30 00:00:00

abstract：:Four-dimensional (4D) bioprinting, encompassing a wide range of disciplines including bioengineering, materials science, chemistry, and computer sciences, is emerging as the next-generation biofabrication technology. By utilizing stimuli-responsive materials and advanced three-dimensional (3D) bioprinting strategies, ...

journal_title：Biofabrication

authors： Li YC,Zhang YS,Akpek A,Shin SR,Khademhosseini A

更新日期：2016-12-02 00:00:00

abstract：:The high attrition rate of neuro-pharmaceuticals as they proceed to market necessitates the development of clinically-relevant in vitro neural microphysiological systems that can be utilized during the preclinical screening phase to assess the safety and efficacy of potential compounds. Historically, proposed models h...

journal_title：Biofabrication

authors： Bowser DA,Moore MJ

更新日期：2019-10-21 00:00:00

abstract：:Laser-assisted bioprinting (LaBP) allows the realization of computer-generated 3D tissue grafts consisting of cells embedded in a hydrogel environment. In this study, human adipose-derived stem cells (hASCs) were printed in a free-scalable 3D grid pattern by means of LaBP. We demonstrate that neither the proliferation...

journal_title：Biofabrication

authors： Gruene M,Pflaum M,Deiwick A,Koch L,Schlie S,Unger C,Wilhelmi M,Haverich A,Chichkov BN

更新日期：2011-03-01 00:00:00

abstract：:Cell therapy represents a promising option for revascularization of ischemic tissues. However, injection of dispersed cells is not optimal to ensure precise homing into the recipient's vasculature. Implantation of cell-engineered scaffolds around the occluded artery may obviate these limitations. Here, we employed the...

journal_title：Biofabrication

authors： Carrabba M,De Maria C,Oikawa A,Reni C,Rodriguez-Arabaolaza I,Spencer H,Slater S,Avolio E,Dang Z,Spinetti G,Madeddu P,Vozzi G

更新日期：2016-03-24 00:00:00

abstract：:Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different p...

journal_title：Biofabrication

authors： Hendrikson WJ,Rouwkema J,Clementi F,van Blitterswijk CA,Farè S,Moroni L

更新日期：2017-08-02 00:00:00

abstract：:Three-dimensional (3D) tissue models are invaluable tools that can closely reflect the in vivo physiological environment. However, they are usually difficult to develop, have a low throughput and are often costly; limiting their utility to most laboratories. The recent availability of inexpensive additive manufacturin...

journal_title：Biofabrication

authors： Flood P,Alvarez L,Reynaud EG

更新日期：2016-10-11 00:00:00

abstract：:Four-dimensional (4D) bioprinting of cell-laden constructs with programmable shape-morphing structures has gained increasing attention in the field of biofabrication and tissue engineering. Currently, most of the widely used materials for 4D printing, including N-isopropylacrylamide-based polymers, are not commonly us...

journal_title：Biofabrication

authors： Luo Y,Lin X,Chen B,Wei X

更新日期：2019-09-13 00:00:00

abstract：:In this paper we explore the printability of reversible networks formed by catechol functionalized PEG solutions and metal cations (Al3+, Fe3+ or V3+). The printability and shape fidelity were dependent on the ink composition (metal ion type, pH, PEG molecular weight) and printing parameters (extrusion pressure and pr...

journal_title：Biofabrication

authors： Włodarczyk-Biegun MK,Paez JI,Villiou M,Feng J,Del Campo A

更新日期：2020-04-29 00:00:00

abstract：:Electrospun fibers of natural polymers are desirable for biomedical applications such as tissue engineering. Crosslinking of electrospun fibers of natural polymers is needed to prevent dissolution in water and to enhance mechanical strength. In this study, an in situ UV-crosslinking method was developed for crosslinki...

journal_title：Biofabrication

authors： Lin WH,Tsai WB

更新日期：2013-09-01 00:00:00

abstract：:Cell cultures for tissue engineering are traditionally prepared on two-dimensional or three-dimensional scaffolds with simple pores; however, this limits mass transportation, which is necessary for cell viability and function. In this paper, an innovative method is proposed for fabricating porous scaffolds with design...

journal_title：Biofabrication

authors： Liu Y,Li X,Qu X,Zhu L,He J,Zhao Q,Wu W,Li D

更新日期：2012-03-01 00:00:00

abstract：:As an alternative to conventional cell culture and animal testing, an organ-on-a-chip is applied to study the biological phenomena of organ development and disease, as well as the interactions between human tissues and external stimuli such as chemicals, forces and electricity. The pattern design of a microfluidic cha...

journal_title：Biofabrication

authors： Mao M,He J,Lu Y,Li X,Li T,Zhou W,Li D

更新日期：2018-02-05 00:00:00

abstract：:Tissue rings with incorporated microscaffolds have been engineered as promising building blocks for constructing biological tubes from the bottom up. However, the microscaffolds available for incorporation are very limited at present. In this paper we provide an efficient strategy to first incorporate microfluidic spu...

journal_title：Biofabrication

authors： Sun T,Shi Q,Yao Y,Sun J,Wang H,Huang Q,Fukuda T

更新日期：2019-06-25 00:00:00

abstract：:Human induced pluripotent stem cells (hiPSCs) can be differentiated at high efficiency into cells of a targeting type but the resulting cell population has to be of high purity for clinical therapies to avoid teratomas. Herein, we report a microfluidic device with integrated and surface functionalised fishnet-like str...

journal_title：Biofabrication

authors： Li X,Yu L,Li J,Minami I,Nakajima M,Noda Y,Kotera H,Liu L,Chen Y

更新日期：2016-09-08 00:00:00