An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues.

abstract：Background:Charcoal rot is a fungal disease that thrives in warm dry conditions and affects the yield of soybeans and other important agronomic crops worldwide. There is a need for robust, automatic and consistent early detection and quantification of disease symptoms which are important in breeding programs for the de...

journal_title：Plant methods

authors： Nagasubramanian K,Jones S,Sarkar S,Singh AK,Singh A,Ganapathysubramanian B

更新日期：2018-10-03 00:00:00

abstract：BACKGROUND:Transient gene expression systems are indispensable tools in molecular biology. Yet, their routine application is limited to few plant species often requiring substantial equipment and facilities. High chloroplast and chlorophyll content may further impede downstream applications of transformed cells from gr...

journal_title：Plant methods

authors： Pitzschke A,Persak H

更新日期：2012-05-04 00:00:00

abstract：BACKGROUND:The inability to genetically transform any fern species has been a major technical barrier to unlocking fern biology. Initial attempts to overcome this limitation were based on transient transformation approaches or achieved very low efficiencies. A highly efficient method of stable transformation was recent...

journal_title：Plant methods

authors： Plackett AR,Rabbinowitsch EH,Langdale JA

更新日期：2015-07-03 00:00:00

abstract：Background:The demand for effective use of water resources has increased because of ongoing global climate transformations in the agriculture science sector. Cost-effective and timely distributions of the appropriate amount of water are vital not only to maintain a healthy status of plants leaves but to drive the produ...

journal_title：Plant methods

authors： Zahid A,Abbas HT,Ren A,Zoha A,Heidari H,Shah SA,Imran MA,Alomainy A,Abbasi QH

更新日期：2019-11-18 00:00:00

abstract：BACKGROUND:Maize haploid breeding technology can be used to rapidly develop homozygous lines, significantly shorten the breeding cycle and improve breeding efficiency. Rapid and accurate sorting haploid kernels is a prerequisite for the large-scale application of this technology. At present, the automatic haploid sorti...

journal_title：Plant methods

authors： Qu Y,Liu Z,Zhang Y,Yang J,Li H

更新日期：2021-01-06 00:00:00

abstract：Background:Wheat is one of the most widely grown crop in temperate climates for food and animal feed. In order to meet the demands of the predicted population increase in an ever-changing climate, wheat production needs to dramatically increase. Spike and grain traits are critical determinants of final yield and grain ...

journal_title：Plant methods

authors： Hughes N,Askew K,Scotson CP,Williams K,Sauze C,Corke F,Doonan JH,Nibau C

更新日期：2017-11-01 00:00:00

abstract：BACKGROUND:Leaf rust (LR), caused by Puccinia triticina and is an important disease of wheat (Triticum aestivum L.). The most sustainable method for controlling rust diseases is deployment of cultivars incorporating adult plant resistance (APR). However, phenotyping breeding populations or germplasm collections for res...

journal_title：Plant methods

authors： Riaz A,Periyannan S,Aitken E,Hickey L

更新日期：2016-03-02 00:00:00

abstract：:[This corrects the article DOI: 10.1186/s13007-019-0478-9.]. ...

journal_title：Plant methods

authors： Dobbels AA,Lorenz AJ

更新日期：2019-10-10 00:00:00

abstract：BACKGROUND:Transcription factors (TFs) coordinate precise gene expression patterns that give rise to distinct phenotypic outputs. The identification of genes and transcriptional networks regulated by a TF often requires stable transformation and expression changes in plant cells. However, the production of stable trans...

journal_title：Plant methods

authors： Bond DM,Albert NW,Lee RH,Gillard GB,Brown CM,Hellens RP,Macknight RC

更新日期：2016-10-19 00:00:00

abstract：:Receptor-like protein kinases (RLKs) are a large and important group of plant proteins involved in numerous aspects of development and stress response. Within this family, homo-oligermization of receptors followed by autophosphorylation of the intracellular protein kinase domain appears to be a widespread mechanism to...

journal_title：Plant methods

authors： Taylor I,Seitz K,Bennewitz S,Walker JC

更新日期：2013-06-26 00:00:00

abstract：Background:Roses are widely used as garden ornamental plants and cut flowers. Rosa chinensis cv 'Old Blush' has been used as a model genotype in rose studies due to its contribution to recurrent flowering and tea scent traits of modern roses. The deficiency of efficient genetic transformation systems is a handicap limi...

journal_title：Plant methods

authors： Lu J,Bai M,Ren H,Liu J,Wang C

更新日期：2017-12-22 00:00:00

abstract：Background:Non-contact resonant ultrasound spectroscopy (NC-RUS) has been proven as a reliable technique for the dynamic determination of leaf water status. It has been already tested in more than 50 plant species. In parallel, relative water content (RWC) is highly used in the ecophysiological field to describe the de...

journal_title：Plant methods

authors： Fariñas MD,Jimenez-Carretero D,Sancho-Knapik D,Peguero-Pina JJ,Gil-Pelegrín E,Gómez Álvarez-Arenas T

更新日期：2019-11-07 00:00:00

abstract：Background:Lignocellulosic biomass is a complex network of polymers making the cell walls of plants. It represents a feedstock of sustainable resources to be converted into fuels, chemicals and materials. Because of its complex architecture, lignocellulose is a recalcitrant material that necessitates some pretreatments...

journal_title：Plant methods

authors： Terryn C,Paës G,Spriet C

更新日期：2018-08-27 00:00:00

abstract：BACKGROUND:The data generated during a course of a biological experiment/study can be sometimes be massive and its management becomes quite critical for the success of the investigation undertaken. The accumulation and analysis of such large datasets often becomes tedious for biologists and lab technicians. Most of the...

journal_title：Plant methods

authors： Vankadavath RN,Hussain AJ,Bodanapu R,Kharshiing E,Basha PO,Gupta S,Sreelakshmi Y,Sharma R

更新日期：2009-12-10 00:00:00

abstract：Background:Measurements of rind and culm thickness and stem radius/diameter are important to biomechanical, ecological and physiological plant studies. However, many methods of measuring rind thickness and diameter are labor intensive and induce plant fatality. A novel rind puncture methodology for obtaining measuremen...

journal_title：Plant methods

authors： Seegmiller WH,Graves J,Robertson DJ

更新日期：2020-04-01 00:00:00

abstract：:Plants are good sources of biologically active compounds with antimicrobial activity, including polypeptides. Antimicrobial peptides (AMPs) represent one of the main barriers of plant innate immunity to environmental stress factors and are attracting much research interest. There are some extraction methods for isolat...

journal_title：Plant methods

authors： Barashkova AS,Rogozhin EA

更新日期：2020-10-23 00:00:00

abstract：BACKGROUND:Pentatricopeptide-repeat proteins (PPRs) characterized by tandem arrays of a degenerate 35-amino-acid repeat (PPR motif) can bind a single strand RNA and regulate organelle gene expression at the post-transcriptional level, including RNA cleavage, splicing, editing and stability etc. PPRs are conserved in al...

journal_title：Plant methods

authors： Chen J,Zhu H,Huang J,Huang W

更新日期：2020-11-18 00:00:00

abstract：BACKGROUND:In eukaryotic cells, the proteasome maintains homeostasis by selectively degrading regulatory and misfolded proteins, and in doing so contributes to the amino acid pool. Inhibition of the proteasome in yeast and human cells decreases de novo protein synthesis. However, it is not know if proteasome inhibition...

journal_title：Plant methods

authors： Van Hoewyk D

更新日期：2016-03-16 00:00:00

abstract：Background:Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very young plants not grown in soil and not allowing full development of the root system. Results:This s...

journal_title：Plant methods

authors： Chen S,Svane SF,Thorup-Kristensen K

更新日期：2019-12-09 00:00:00

abstract：Background:Open tissue culture technique could be simplified by using different bacteriostatic agents. There is a great difference in the bacteriostatic effects of different antimicrobial agents on various explants. However, there is no report about the effective bacteriostatic agent for open tissue culture of Acacia a...

journal_title：Plant methods

authors： Huang L,Wang H,Shahid MQ,Zeng B

更新日期：2019-01-25 00:00:00

abstract：BACKGROUND:Nectar reabsorption is a widely known phenomenon, related to the strategy of resource-recovery and also to maintain the nectar homeostasis at the nectary. The method currently performed to demonstrate nectar being reabsorbed involves the use of radioactive tracers applied to the nectary. Although this method...

journal_title：Plant methods

authors： Cardoso-Gustavson P,Robazzi Bignelli Valente Aguiar JM,Ricardo Pansarin E,de Barros F

更新日期：2013-06-19 00:00:00

abstract：:[This corrects the article DOI: 10.1186/s13007-017-0166-6.]. ...

journal_title：Plant methods

authors： Lüpke M,Steinbrecher R,Leuchner M,Menzel A

更新日期：2017-11-16 00:00:00

abstract：Background:Functional genomic studies using genetics approaches of conifers are hampered by the complex and enormous genome, long vegetative growth period, and exertion in genetic transformation. Thus, the research carried out on gene function in Pinus tabuliformis is typically performed by heterologous expression base...

journal_title：Plant methods

authors： Liu S,Ma J,Liu H,Guo Y,Li W,Niu S

更新日期：2020-04-10 00:00:00

abstract：Background:Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate de-noising, and adopting normal-probability statistical estimation models which have few parameters a...

journal_title：Plant methods

authors： Chen Z,Wang F,Zhang P,Ke C,Zhu Y,Cao W,Jiang H

更新日期：2020-02-26 00:00:00

abstract：Background:Root is the principal part of plants to absorb water and nutrient, anchor the plant and affect yield and quality. Non-destructive detection of root traits is difficult to perform due to the hidden nature of the root. Therefore, improved methods to measure roots are necessary to support plant breeding, and op...

journal_title：Plant methods

authors： Lu W,Wang X,Wang F

更新日期：2019-03-23 00:00:00

abstract：BACKGROUND:Fluorescent proteins are extraordinary tools for biology studies due to their versatility; they are used extensively to improve comprehension of plant-microbe interactions. The viral infection process can easily be tracked and imaged in a plant with fluorescent protein-tagged viruses. In plants, fluorescent ...

journal_title：Plant methods

authors： Pasin F,Kulasekaran S,Natale P,Simón-Mateo C,García JA

更新日期：2014-07-05 00:00:00

abstract：Motivation:In 2005, researchers from the French National Research Institute for Agriculture, Food and Environment (Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, INRAE) started a collaboration with the French farmers' seed network Réseau Semences Paysannes (RSP) on bread wheat par...

journal_title：Plant methods

authors： De Oliveira Y,Burlot L,Dawson JC,Goldringer I,Madi D,Rivière P,Steinbach D,van Frank G,Thomas M

更新日期：2020-07-23 00:00:00

abstract：:Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summarizes recent advan...

journal_title：Plant methods

authors： Yang X,Scheffler BE,Weston LA

更新日期：2006-03-01 00:00:00

abstract：Background:Insect herbivory poses a major threat to maize. Benzoxazinoids are important anti-insect secondary metabolites in maize, whose biosynthetic pathway has been extensively studied. However, yet little is known about how benzoxazinoids are regulated in maize, partly due to lack of mutant resources and recalcitra...

journal_title：Plant methods

authors： Gao L,Shen G,Zhang L,Qi J,Zhang C,Ma C,Li J,Wang L,Malook SU,Wu J

更新日期：2019-11-28 00:00:00

abstract：Background:To investigate potential effects of herbicide phytotoxic on crops, a major challenge is a lack of non-destructive and rapid methods to detect plant growth that could allow characterization of herbicide-resistant plants. In such a case, hyperspectral imaging can quickly obtain the spectrum for each pixel in t...

journal_title：Plant methods

authors： Wang J,Zhang C,Shi Y,Long M,Islam F,Yang C,Yang S,He Y,Zhou W

更新日期：2020-03-05 00:00:00