Abstract:
BACKGROUND:Maize haploid breeding technology can be used to rapidly develop homozygous lines, significantly shorten the breeding cycle and improve breeding efficiency. Rapid and accurate sorting haploid kernels is a prerequisite for the large-scale application of this technology. At present, the automatic haploid sorting based on nuclear magnetic resonance (NMR) using a single threshold method has been realized. However, embryo-aborted (EmA) kernels are usually produced during in vivo haploid induction, and both haploids and EmA kernels have lower oil content and are separated together using a single threshold method based on NMR. This leads to a higher haploid false discrimination rate (FDR) and requires secondary manual sorting to select the haploid kernels from the mixtures, which increases the sorting cost and decreases the haploid sorting efficiency. In order to improve the correct discrimination rate (CDR) in sorting haploids, a method to distinguish EmA kernels is required. RESULTS:Single kernel weight and oil content were measured for the diploid, haploid, and EmA kernels derived from three maize hybrids and nine inbred lines by in vivo induction. The results showed that the distribution of oil content showed defined boundaries between the three types of kernels, while the single kernel weight didn't. According to the distribution of oil content in the three types of kernels, a double-threshold method was proposed to distinguish the embryo-aborted kernels, haploid and diploid kernels based on NMR and their oil content. The double thresholds were set based on the minimum oil content of diploid kernels and the maximum content of EmA kernels as the upper and lower boundary values, respectively. The CDR of EmA kernels in different maize materials was > 97.8%, and the average FDR was reduced by 27.9 percent. CONCLUSIONS:The oil content is an appropriate indicator to discriminate diploid, haploid and EmA kernels. An oil content double-threshold method based on NMR was first developed in this study to identify the three types of kernels. This methodology could reduce the FDR of haploids and improve the sorting efficiency of automated sorting system. Thus, this technique represents a potentially efficient method for haploid sorting and provides a reference for the process of automated sorting of haploid kernels with high efficiency using NMR.
journal_name
Plant Methodsjournal_title
Plant methodsauthors
Qu Y,Liu Z,Zhang Y,Yang J,Li Hdoi
10.1186/s13007-020-00703-4subject
Has Abstractpub_date
2021-01-06 00:00:00pages
2issue
1issn
1746-4811pii
10.1186/s13007-020-00703-4journal_volume
17pub_type
杂志文章相关文献
Plant Methods文献大全abstract::Modern agriculture has created a demand for plant biotechnology products that provide durable resistance to insect pests, tolerance of herbicide applications for weed control, and agronomic traits tailored for specific geographies. These transgenic trait products require a modular and sequential multigene stacking pla...
journal_title:Plant methods
pub_type: 杂志文章,评审
doi:10.1186/s13007-016-0111-0
更新日期:2016-02-01 00:00:00
abstract:BACKGROUND:It is well known that preparation of biological (plant and animal) tissues for Scanning Electron Microscopy (SEM) by chemical fixation and critical point drying results in shrinkage of tissues, often by up to 20-30%, depending on the tissue type and fixation protocol used. We sought to identify a protocol th...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-9-36
更新日期:2013-10-02 00:00:00
abstract:BACKGROUND:When conducting plant research, the measurement of photosynthetic pigments can provide basic information on the physiological status of a plant. High-pressure liquid chromatography (HPLC) is becoming widely used for this purpose because it provides an accurate determination of a variety of photosynthetic pig...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-9-19
更新日期:2013-06-19 00:00:00
abstract:BACKGROUND:Despite increasing demand, imaging the internal structure of plant organs or tissues without the use of transgenic lines expressing fluorescent proteins remains a challenge. Techniques such as magnetic resonance imaging, optical projection tomography or X-ray absorption tomography have been used with various...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-015-0098-y
更新日期:2015-12-18 00:00:00
abstract::Current methods of in-house plant phenotyping are providing a powerful new tool for plant biology studies. The self-constructed and commercial platforms established in the last few years, employ non-destructive methods and measurements on a large and high-throughput scale. The platforms offer to certain extent, automa...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-015-0072-8
更新日期:2015-04-17 00:00:00
abstract:Background:Chickpea (Cicer arietinum L.), an important legume crop is one of the major source of dietary protein. Developing an efficient and reproducible transformation method is imperative to expedite functional genomics studies in this crop. Here, we present an optimized and detailed procedure for Agrobacterium rhiz...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-018-0315-6
更新日期:2018-07-06 00:00:00
abstract:Background:Plant roots release a variety of organic compounds into the soil which alter the physical, chemical and biological properties of the rhizosphere. Root exudates are technically challenging to measure in soil because roots are difficult to access and exudates can be bound by minerals or consumed by microorgani...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-018-0380-x
更新日期:2018-12-20 00:00:00
abstract:BACKGROUND:A multitude of different imaging systems are already available to image genetically altered RNA species; however, only a few of these techniques are actually suitable to visualize endogenous RNA. One possibility is to use fluorescently-labelled and hybridization-sensitive probes. In order to yield more infor...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-10-15
更新日期:2014-05-31 00:00:00
abstract:BACKGROUND:Archegoniates (bryophytes, ferns and gymnosperms), such as the moss Physcomitrella patens, possess freely motile sperm cells (spermatozoids) which reach the egg cell via surface water. Although these motile flagellated sperm cells are a traditional botanical subject, they have not been thoroughly analysed in...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-017-0186-2
更新日期:2017-05-10 00:00:00
abstract::Over the last 10 years, high-density SNP arrays and DNA re-sequencing have illuminated the majority of the genotypic space for a number of organisms, including humans, maize, rice and Arabidopsis. For any researcher willing to define and score a phenotype across many individuals, Genome Wide Association Studies (GWAS)...
journal_title:Plant methods
pub_type: 杂志文章,评审
doi:10.1186/1746-4811-9-29
更新日期:2013-07-22 00:00:00
abstract:Background:High throughput non-destructive phenotyping is emerging as a significant approach for phenotyping germplasm and breeding populations for the identification of superior donors, elite lines, and QTLs. Detection and counting of spikes, the grain bearing organs of wheat, is critical for phenomics of a large set ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00582-9
更新日期:2020-03-18 00:00:00
abstract:Motivation:In 2005, researchers from the French National Research Institute for Agriculture, Food and Environment (Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, INRAE) started a collaboration with the French farmers' seed network Réseau Semences Paysannes (RSP) on bread wheat par...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00640-2
更新日期:2020-07-23 00:00:00
abstract:UNLABELLED: BACKGROUND:The powdery mildew disease represents a valuable patho-system to study the interaction between plant hosts and obligate biotrophic fungal pathogens. Numerous discoveries have been made on the basis of the quantitative evaluation of plant-powdery mildew interactions, especially in the context of ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-35
更新日期:2012-08-31 00:00:00
abstract:BACKGROUND:Mitochondria play critical roles in plant growth, development and stress tolerance. Numerous researchers have carried out studies on the plant mitochondrial genome structure, mitochondrial metabolism and nuclear-cytoplasmic interactions. However, classical plant mitochondria extraction methods are time-consu...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00690-6
更新日期:2020-11-04 00:00:00
abstract:BACKGROUND:Transient gene expression systems are indispensable tools in molecular biology. Yet, their routine application is limited to few plant species often requiring substantial equipment and facilities. High chloroplast and chlorophyll content may further impede downstream applications of transformed cells from gr...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-14
更新日期:2012-05-04 00:00:00
abstract:BACKGROUND:There is considerable interest in rapid assays or screening systems for assigning gene function. However, analysis of gene function in the flowers of some species is restricted due to the difficulty of producing stably transformed transgenic plants. As a result, experimental approaches based on transient gen...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-3-1
更新日期:2007-01-08 00:00:00
abstract:BACKGROUND:The inability to genetically transform any fern species has been a major technical barrier to unlocking fern biology. Initial attempts to overcome this limitation were based on transient transformation approaches or achieved very low efficiencies. A highly efficient method of stable transformation was recent...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-015-0080-8
更新日期:2015-07-03 00:00:00
abstract:BACKGROUND:Protein phosphorylation is accepted as a major regulatory pathway in plants. More than 1000 protein kinases are predicted in the Arabidopsis proteome, however, only a few studies look systematically for in vivo protein phosphorylation sites. Owing to the low stoichiometry and low abundance of phosphorylated ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-1-9
更新日期:2005-11-01 00:00:00
abstract:BACKGROUND:Transcription factors (TFs) coordinate precise gene expression patterns that give rise to distinct phenotypic outputs. The identification of genes and transcriptional networks regulated by a TF often requires stable transformation and expression changes in plant cells. However, the production of stable trans...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0141-7
更新日期:2016-10-19 00:00:00
abstract:BACKGROUND:Starch is the most important source of calories for human nutrition and the majority of it is produced by cereal farming. Starch is also used as a renewable raw material in a range of industrial sectors. It can be chemically modified to introduce new physicochemical properties. In this way starch is adapted ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-36
更新日期:2012-09-07 00:00:00
abstract:: Generating and identifying transformants is essential for many studies of gene function. In Arabidopsis thaliana, a revolutionary protocol termed floral dip is now the most widely used transformation method. Although robust, it involves a number of relatively time-consuming and laborious steps, including manipulating...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-5-3
更新日期:2009-02-27 00:00:00
abstract:: Three terrestrial plants are known to perform C4 photosynthesis without the dual-cell system by partitioning two distinct types of chloroplasts in separate cytoplasmic compartments. We report herein a protocol for isolating the dimorphic chloroplasts from Bienertia sinuspersici. Hypo-osmotically lysed protoplasts und...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-8
更新日期:2012-03-06 00:00:00
abstract:Background:Low pollen viability may limit grapevine yield under certain conditions, causing relevant economic losses to grape-growers. It is usually evaluated by the quantification of the number of viable and non-viable pollen grains that are present in a sample after an adequate pollen grain staining procedure. Althou...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-017-0267-2
更新日期:2018-01-09 00:00:00
abstract:BACKGROUND:Brassinosteriods (BRs) are a group of important phytohormones that have major effects on plant growth and development. To fully elucidate the function of BRs, a sensitive BR assay is required. However, most of the previously reported methods are tedious and time-consuming due to multiple pretreatment steps. ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-10-39
更新日期:2014-11-24 00:00:00
abstract:BACKGROUND:Kinome profiling aims at the parallel analysis of kinase activities in a cell. Novel developed arrays containing consensus substrates for kinases are used to assess those kinase activities. The arrays described in this paper were already used to determine kinase activities in mammalian systems, but since sub...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-3-3
更新日期:2007-02-12 00:00:00
abstract:BACKGROUND:Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity, and in vivo protein-protein i...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-5-16
更新日期:2009-11-24 00:00:00
abstract:BACKGROUND:Cyst nematodes are biotrophs that form specialized feeding structures in the roots of host plants, which consist of a syncytial fusion of hypertrophied cells. The formation of syncytium is accompanied by profound transcriptional changes and active metabolism in infected tissues. The challenge in gene express...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0123-9
更新日期:2016-04-26 00:00:00
abstract:BACKGROUND:In eukaryotic cells, the proteasome maintains homeostasis by selectively degrading regulatory and misfolded proteins, and in doing so contributes to the amino acid pool. Inhibition of the proteasome in yeast and human cells decreases de novo protein synthesis. However, it is not know if proteasome inhibition...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0120-z
更新日期:2016-03-16 00:00:00
abstract:: Research in plant molecular biology involves DNA purification on a daily basis. Although different commercial kits enable convenient extraction of high-quality DNA from E. coli cells, PCR and agarose gel samples as well as plant tissues, each kit is designed for a particular type of DNA extraction work, and the cost ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-6-1
更新日期:2010-01-14 00:00:00
abstract:Background:In-field measurement of yield and growth rate in pasture species is imprecise and costly, limiting scientific and commercial application. Our study proposed a LiDAR-based mobile platform for non-invasive vegetative biomass and growth rate estimation in perennial ryegrass (Lolium perenne L.). This included de...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0456-2
更新日期:2019-07-10 00:00:00