Abstract:
UNLABELLED: BACKGROUND:The powdery mildew disease represents a valuable patho-system to study the interaction between plant hosts and obligate biotrophic fungal pathogens. Numerous discoveries have been made on the basis of the quantitative evaluation of plant-powdery mildew interactions, especially in the context of hyper-susceptible and/or resistant plant mutants. However, the presently available methods to score the pathogenic success of powdery mildew fungi are laborious and thus not well suited for medium- to high-throughput analysis. RESULTS:Here we present two new protocols that allow the rapid quantitative assessment of powdery mildew disease development. One procedure depends on quantitative polymerase chain reaction (qPCR)-based evaluation of fungal biomass, while the other relies on the quantification of fungal conidiospores. We validated both techniques using the powdery mildew pathogen Golovinomyces orontii on a set of hyper-susceptible and resistant Arabidopsis thaliana mutants and found that both cover a wide dynamic range of one to two (qPCR) and four to five (quantification of conidia) orders of magnitude, respectively. The two approaches yield reproducible results and are easy to perform without specialized equipment. CONCLUSIONS:The qPCR and spore count assays rapidly and reproducibly quantify powdery mildew pathogenesis. Our methods are performed at later stages of infection and discern mutant phenotypes accurately. The assays therefore complement currently used procedures of powdery mildew quantification and can overcome some of their limitations. In addition, they can easily be adapted to other plant-powdery mildew patho-systems.
journal_name
Plant Methodsjournal_title
Plant methodsauthors
Weßling R,Panstruga Rdoi
10.1186/1746-4811-8-35subject
Has Abstractpub_date
2012-08-31 00:00:00pages
35issue
1issn
1746-4811pii
1746-4811-8-35journal_volume
8pub_type
杂志文章相关文献
Plant Methods文献大全abstract:BACKGROUND:Transcription factors (TFs) coordinate precise gene expression patterns that give rise to distinct phenotypic outputs. The identification of genes and transcriptional networks regulated by a TF often requires stable transformation and expression changes in plant cells. However, the production of stable trans...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0141-7
更新日期:2016-10-19 00:00:00
abstract:Background:Chloroplasts are critical organelles that perceive and convey metabolic and stress signals to different cellular components, while remaining the seat of photosynthesis and a metabolic factory. The proteomes of intact leaves, chloroplasts, and suborganellar fractions of plastids have been evaluated in the mod...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00667-5
更新日期:2020-09-24 00:00:00
abstract:BACKGROUND:Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity, and in vivo protein-protein i...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-5-16
更新日期:2009-11-24 00:00:00
abstract:Background:Most bacteria are not culturable, but can be identified through molecular methods such as metagenomics studies. Due to specific metabolic requirements and symbiotic relationships, these bacteria cannot survive on typical laboratory media. Many economically and medically important bacteria are unculturable; i...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0465-1
更新日期:2019-07-31 00:00:00
abstract:BACKGROUND:Genetic studies on the molecular mechanisms of the regulation of root growth require the characterisation of a specific root phenotype to be linked with a certain genotype. Such studies using classical labour-intensive methods are severely hindered due to the technical limitations that are associated with th...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0135-5
更新日期:2016-06-24 00:00:00
abstract:BACKGROUND:Nectar reabsorption is a widely known phenomenon, related to the strategy of resource-recovery and also to maintain the nectar homeostasis at the nectary. The method currently performed to demonstrate nectar being reabsorbed involves the use of radioactive tracers applied to the nectary. Although this method...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-9-20
更新日期:2013-06-19 00:00:00
abstract:BACKGROUND:Microsatellites are popular molecular markers in many plant species due to their stable and highly polymorphic nature. A number of analysis methods have been described but analyses of these markers are typically performed on cumbersome polyacrylamide gels or more conveniently by capillary electrophoresis on ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-4-8
更新日期:2008-05-19 00:00:00
abstract:Background:Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under fluctuating light despite being indistinguishable from wild-type plants under standard constant light...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0546-1
更新日期:2019-12-23 00:00:00
abstract:Background:Root is the principal part of plants to absorb water and nutrient, anchor the plant and affect yield and quality. Non-destructive detection of root traits is difficult to perform due to the hidden nature of the root. Therefore, improved methods to measure roots are necessary to support plant breeding, and op...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0414-z
更新日期:2019-03-23 00:00:00
abstract:BACKGROUND:Plant metabolites are commonly functionally classified, as defense- or growth-related phytohormones, primary and specialized metabolites, and so forth. Analytical procedures for the quantifications of these metabolites are challenging because the metabolites can vary over several orders of magnitude in conce...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0130-x
更新日期:2016-05-26 00:00:00
abstract:Background:Plant root apex is the major part to direct the root growth and development by responding to various signals/cues from internal and soil environments. To study and understand root system biology particularly at a molecular and cellular level, an Arabidopsis T-DNA insertional enhancer trap line J3411 expressi...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0393-0
更新日期:2019-01-31 00:00:00
abstract:Background:The stomata of plants mainly regulate gas exchange and water dispersion between the interior and external environments of plants and play a major role in the plants' health. The existing methods of stomata segmentation and measurement are mostly for specialized plants. The purpose of this research is to deve...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0453-5
更新日期:2019-07-03 00:00:00
abstract:BACKGROUND:Suppression subtractive hybridization is a popular technique for gene discovery from non-model organisms without an annotated genome sequence, such as cowpea (Vigna unguiculata (L.) Walp). We aimed to use this method to enrich for genes expressed during drought stress in a drought tolerant cowpea line. Howev...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-6-10
更新日期:2010-04-01 00:00:00
abstract:: Generating and identifying transformants is essential for many studies of gene function. In Arabidopsis thaliana, a revolutionary protocol termed floral dip is now the most widely used transformation method. Although robust, it involves a number of relatively time-consuming and laborious steps, including manipulating...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-5-3
更新日期:2009-02-27 00:00:00
abstract:Background:Plasmopara obducens is the biotrophic oomycete responsible for impatiens downy mildew, a destructive disease of Impatiens that causes high crop loss. Currently, there are no available methods for the microscopic detection of P. obducens from leaves of impatiens, which may be contributing to the spread of the...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-018-0362-z
更新日期:2018-10-24 00:00:00
abstract:BACKGROUND:Cyst nematodes are biotrophs that form specialized feeding structures in the roots of host plants, which consist of a syncytial fusion of hypertrophied cells. The formation of syncytium is accompanied by profound transcriptional changes and active metabolism in infected tissues. The challenge in gene express...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0123-9
更新日期:2016-04-26 00:00:00
abstract:Background:The demand for effective use of water resources has increased because of ongoing global climate transformations in the agriculture science sector. Cost-effective and timely distributions of the appropriate amount of water are vital not only to maintain a healthy status of plants leaves but to drive the produ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0522-9
更新日期:2019-11-18 00:00:00
abstract:BACKGROUND:Leaf rust (LR), caused by Puccinia triticina and is an important disease of wheat (Triticum aestivum L.). The most sustainable method for controlling rust diseases is deployment of cultivars incorporating adult plant resistance (APR). However, phenotyping breeding populations or germplasm collections for res...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0117-7
更新日期:2016-03-02 00:00:00
abstract:Background:Protein data over circadian time scale is scarce for clock transcription factors. Further work in this direction is required for refining quantitative clock models. However, gathering highly resolved dynamics of low-abundance transcription factors has been a major challenge in the field. In this work we prov...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0454-4
更新日期:2019-07-08 00:00:00
abstract:BACKGROUND:Striga species are noxious root hemi-parasitic weeds that debilitate cereal production in sub-Saharan Africa (SSA). Control options for Striga are limited and developing Striga resistant crop germplasm is regarded as the best and most sustainable control measure. Efforts to improve germplasm for Striga resis...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-20
更新日期:2012-06-21 00:00:00
abstract:: Microarrays are routine tools for transcript profiling, and genomic tiling arrays such as the Arabidopsis AGRONOMICS1 arrays have been found to be highly suitable for such experiments because changes in genome annotation can be easily integrated at the data analysis level. In a transcript profiling experiment, RNA la...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-18
更新日期:2012-06-13 00:00:00
abstract:Background:Open tissue culture technique could be simplified by using different bacteriostatic agents. There is a great difference in the bacteriostatic effects of different antimicrobial agents on various explants. However, there is no report about the effective bacteriostatic agent for open tissue culture of Acacia a...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0390-3
更新日期:2019-01-25 00:00:00
abstract:BACKGROUND:Establishment and maintenance of mutualistic plant-microbial interactions in the rhizosphere and within plant roots involve several root cell types. The processes of host-microbe recognition and infection require complex signal exchange and activation of downstream responses. These molecular events coordinat...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0105-y
更新日期:2016-01-22 00:00:00
abstract:BACKGROUND:In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made to characterise such putative plant channels at the functional level have most often relied on electrophysiological analyses in classical expre...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-1-14
更新日期:2005-12-19 00:00:00
abstract::Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the power and raw materials that can be used as sophisticated biological factories. They are rich in energy as they have lots of pigment-protein complexes capable of collecting sunlight, in sugar produced by photosynthesis and in m...
journal_title:Plant methods
pub_type: 杂志文章,评审
doi:10.1186/s13007-017-0179-1
更新日期:2017-04-19 00:00:00
abstract:Background:Plant parasitic weeds belonging to the genus Striga are a major threat for food production in Sub-Saharan Africa and Southeast Asia. The parasite's life cycle starts with the induction of seed germination by host plant-derived signals, followed by parasite attachment, infection, outgrowth, flowering, reprodu...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00602-8
更新日期:2020-05-01 00:00:00
abstract::Plants are good sources of biologically active compounds with antimicrobial activity, including polypeptides. Antimicrobial peptides (AMPs) represent one of the main barriers of plant innate immunity to environmental stress factors and are attracting much research interest. There are some extraction methods for isolat...
journal_title:Plant methods
pub_type: 杂志文章,评审
doi:10.1186/s13007-020-00687-1
更新日期:2020-10-23 00:00:00
abstract:BACKGROUND:Starch is the most important source of calories for human nutrition and the majority of it is produced by cereal farming. Starch is also used as a renewable raw material in a range of industrial sectors. It can be chemically modified to introduce new physicochemical properties. In this way starch is adapted ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-36
更新日期:2012-09-07 00:00:00
abstract:BACKGROUND:Artificial chromosomes (ACs) are a promising next-generation vector for genetic engineering. The most common methods for developing AC constructs are to clone and combine centromeric DNA and telomeric DNA fragments into a single large DNA construct. The AC constructs developed from such methods will contain ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-7-10
更新日期:2011-04-15 00:00:00
abstract:BACKGROUND:A multitude of different imaging systems are already available to image genetically altered RNA species; however, only a few of these techniques are actually suitable to visualize endogenous RNA. One possibility is to use fluorescently-labelled and hybridization-sensitive probes. In order to yield more infor...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-10-15
更新日期:2014-05-31 00:00:00