Protocol: genetic transformation of the fern Ceratopteris richardii through microparticle bombardment.

abstract：Background:To investigate potential effects of herbicide phytotoxic on crops, a major challenge is a lack of non-destructive and rapid methods to detect plant growth that could allow characterization of herbicide-resistant plants. In such a case, hyperspectral imaging can quickly obtain the spectrum for each pixel in t...

journal_title：Plant methods

authors： Wang J,Zhang C,Shi Y,Long M,Islam F,Yang C,Yang S,He Y,Zhou W

更新日期：2020-03-05 00:00:00

abstract：BACKGROUND:Starch is the most important source of calories for human nutrition and the majority of it is produced by cereal farming. Starch is also used as a renewable raw material in a range of industrial sectors. It can be chemically modified to introduce new physicochemical properties. In this way starch is adapted ...

journal_title：Plant methods

authors： Carciofi M,Blennow A,Nielsen MM,Holm PB,Hebelstrup KH

更新日期：2012-09-07 00:00:00

abstract：BACKGROUND:Establishing transcriptional regulatory networks that include protein-protein and protein-DNA interactions has become a key component to better understanding many fundamental biological processes. Although a variety of techniques are available to expose protein-protein and protein-DNA interactions, unequivoc...

journal_title：Plant methods

authors： Xie Z,Grotewold E

更新日期：2008-10-27 00:00:00

abstract：Background:Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very young plants not grown in soil and not allowing full development of the root system. Results:This s...

journal_title：Plant methods

authors： Chen S,Svane SF,Thorup-Kristensen K

更新日期：2019-12-09 00:00:00

abstract：BACKGROUND:Genetic studies on the molecular mechanisms of the regulation of root growth require the characterisation of a specific root phenotype to be linked with a certain genotype. Such studies using classical labour-intensive methods are severely hindered due to the technical limitations that are associated with th...

journal_title：Plant methods

authors： Slota M,Maluszynski M,Szarejko I

更新日期：2016-06-24 00:00:00

abstract：BACKGROUND:The tomato (Solanum lycopersicum L.) plant is both an economically important food crop and an ideal dicot model to investigate various physiological phenomena not possible in Arabidopsis thaliana. Due to the great diversity of tomato cultivars used by the research community, it is often difficult to reliably...

journal_title：Plant methods

authors： Carvalho RF,Campos ML,Pino LE,Crestana SL,Zsögön A,Lima JE,Benedito VA,Peres LE

更新日期：2011-06-29 00:00:00

abstract：Background:Open tissue culture technique could be simplified by using different bacteriostatic agents. There is a great difference in the bacteriostatic effects of different antimicrobial agents on various explants. However, there is no report about the effective bacteriostatic agent for open tissue culture of Acacia a...

journal_title：Plant methods

authors： Huang L,Wang H,Shahid MQ,Zeng B

更新日期：2019-01-25 00:00:00

abstract：: Research in plant molecular biology involves DNA purification on a daily basis. Although different commercial kits enable convenient extraction of high-quality DNA from E. coli cells, PCR and agarose gel samples as well as plant tissues, each kit is designed for a particular type of DNA extraction work, and the cost ...

journal_title：Plant methods

authors： Li JF,Li L,Sheen J

更新日期：2010-01-14 00:00:00

abstract：BACKGROUND:Maize haploid breeding technology can be used to rapidly develop homozygous lines, significantly shorten the breeding cycle and improve breeding efficiency. Rapid and accurate sorting haploid kernels is a prerequisite for the large-scale application of this technology. At present, the automatic haploid sorti...

journal_title：Plant methods

authors： Qu Y,Liu Z,Zhang Y,Yang J,Li H

更新日期：2021-01-06 00:00:00

abstract：Background:Many agronomic traits have been bred into modern wheat varieties, but wheat (Triticum aestivum L.) continues to be vulnerable to heat stress, with high night-time temperature (HNT) stress shown to have large negative impact on yield and quality. Global mean temperature during the day is consistently warming ...

journal_title：Plant methods

authors： Hein NT,Wagner D,Bheemanahalli R,Šebela D,Bustamante C,Chiluwal A,Neilsen ML,Jagadish SVK

更新日期：2019-04-24 00:00:00

abstract：BACKGROUND:Artificial chromosomes (ACs) are a promising next-generation vector for genetic engineering. The most common methods for developing AC constructs are to clone and combine centromeric DNA and telomeric DNA fragments into a single large DNA construct. The AC constructs developed from such methods will contain ...

journal_title：Plant methods

authors： Lin L,Koo DH,Zhang W,St Peter J,Jiang J

更新日期：2011-04-15 00:00:00

abstract：Background:The stomata of plants mainly regulate gas exchange and water dispersion between the interior and external environments of plants and play a major role in the plants' health. The existing methods of stomata segmentation and measurement are mostly for specialized plants. The purpose of this research is to deve...

journal_title：Plant methods

authors： Li K,Huang J,Song W,Wang J,Lv S,Wang X

更新日期：2019-07-03 00:00:00

abstract：Background:The demand for effective use of water resources has increased because of ongoing global climate transformations in the agriculture science sector. Cost-effective and timely distributions of the appropriate amount of water are vital not only to maintain a healthy status of plants leaves but to drive the produ...

journal_title：Plant methods

authors： Zahid A,Abbas HT,Ren A,Zoha A,Heidari H,Shah SA,Imran MA,Alomainy A,Abbasi QH

更新日期：2019-11-18 00:00:00

abstract：Background:Wheat is one of the most widely grown crop in temperate climates for food and animal feed. In order to meet the demands of the predicted population increase in an ever-changing climate, wheat production needs to dramatically increase. Spike and grain traits are critical determinants of final yield and grain ...

journal_title：Plant methods

authors： Hughes N,Askew K,Scotson CP,Williams K,Sauze C,Corke F,Doonan JH,Nibau C

更新日期：2017-11-01 00:00:00

abstract：BACKGROUND:The data generated during a course of a biological experiment/study can be sometimes be massive and its management becomes quite critical for the success of the investigation undertaken. The accumulation and analysis of such large datasets often becomes tedious for biologists and lab technicians. Most of the...

journal_title：Plant methods

authors： Vankadavath RN,Hussain AJ,Bodanapu R,Kharshiing E,Basha PO,Gupta S,Sreelakshmi Y,Sharma R

更新日期：2009-12-10 00:00:00

abstract：Background:Monitoring the timing of seedling emergence and early development via high-throughput phenotyping with computer vision is a challenging topic of high interest in plant science. While most studies focus on the measurements of leaf area index or detection of specific events such as emergence, little attention ...

journal_title：Plant methods

authors： Samiei S,Rasti P,Ly Vu J,Buitink J,Rousseau D

更新日期：2020-07-30 00:00:00

abstract：Background:Plastome (plastid genome) sequences provide valuable information for understanding the phylogenetic relationships and evolutionary history of plants. Although the rapid development of high-throughput sequencing technology has led to an explosion of plastome sequences, annotation remains a significant bottlen...

journal_title：Plant methods

authors： Qu XJ,Moore MJ,Li DZ,Yi TS

更新日期：2019-05-21 00:00:00

abstract：Background:Lignocellulosic biomass is a complex network of polymers making the cell walls of plants. It represents a feedstock of sustainable resources to be converted into fuels, chemicals and materials. Because of its complex architecture, lignocellulose is a recalcitrant material that necessitates some pretreatments...

journal_title：Plant methods

authors： Terryn C,Paës G,Spriet C

更新日期：2018-08-27 00:00:00

abstract：Background:Most bacteria are not culturable, but can be identified through molecular methods such as metagenomics studies. Due to specific metabolic requirements and symbiotic relationships, these bacteria cannot survive on typical laboratory media. Many economically and medically important bacteria are unculturable; i...

journal_title：Plant methods

authors： Krystel J,Shi Q,Shaw J,Gupta G,Hall D,Stover E

更新日期：2019-07-31 00:00:00

abstract：BACKGROUND:Plant metabolites are commonly functionally classified, as defense- or growth-related phytohormones, primary and specialized metabolites, and so forth. Analytical procedures for the quantifications of these metabolites are challenging because the metabolites can vary over several orders of magnitude in conce...

journal_title：Plant methods

authors： Schäfer M,Brütting C,Baldwin IT,Kallenbach M

更新日期：2016-05-26 00:00:00

abstract：Background:Progress in high-throughput molecular methods accompanied by more complex experimental designs demands novel data visualisation solutions. To specifically answer the question which parts of the specifical biological system are responding in particular perturbation, integrative approach in which experimental ...

journal_title：Plant methods

authors： Zagorščak M,Blejec A,Ramšak Ž,Petek M,Stare T,Gruden K

更新日期：2018-08-30 00:00:00

abstract：BACKGROUND:Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity, and in vivo protein-protein i...

journal_title：Plant methods

authors： Wu FH,Shen SC,Lee LY,Lee SH,Chan MT,Lin CS

更新日期：2009-11-24 00:00:00

abstract：:Current methods of in-house plant phenotyping are providing a powerful new tool for plant biology studies. The self-constructed and commercial platforms established in the last few years, employ non-destructive methods and measurements on a large and high-throughput scale. The platforms offer to certain extent, automa...

journal_title：Plant methods

authors： Humplík JF,Lazár D,Husičková A,Spíchal L

更新日期：2015-04-17 00:00:00

abstract：BACKGROUND:We previously developed a high-throughput system called 'Ice-Cap' for growing Arabidopsis seedlings in a 96-well format and rapidly collecting tissue for subsequent DNA extraction and genotyping. While the originally described Ice-Cap method is an effective tool for high-throughput genotyping, one shortcomin...

journal_title：Plant methods

authors： Clark KA,Krysan PJ

更新日期：2007-06-12 00:00:00

abstract：: Microarrays are routine tools for transcript profiling, and genomic tiling arrays such as the Arabidopsis AGRONOMICS1 arrays have been found to be highly suitable for such experiments because changes in genome annotation can be easily integrated at the data analysis level. In a transcript profiling experiment, RNA la...

journal_title：Plant methods

authors： Müller M,Patrignani A,Rehrauer H,Gruissem W,Hennig L

更新日期：2012-06-13 00:00:00

abstract：BACKGROUND:Cyst nematodes are biotrophs that form specialized feeding structures in the roots of host plants, which consist of a syncytial fusion of hypertrophied cells. The formation of syncytium is accompanied by profound transcriptional changes and active metabolism in infected tissues. The challenge in gene express...

journal_title：Plant methods

authors： Anjam MS,Ludwig Y,Hochholdinger F,Miyaura C,Inada M,Siddique S,Grundler FM

更新日期：2016-04-26 00:00:00

abstract：BACKGROUND:A multitude of different imaging systems are already available to image genetically altered RNA species; however, only a few of these techniques are actually suitable to visualize endogenous RNA. One possibility is to use fluorescently-labelled and hybridization-sensitive probes. In order to yield more infor...

journal_title：Plant methods

authors： Göhring J,Fulcher N,Schilcher K,Barta A,Jacak J

更新日期：2014-05-31 00:00:00

abstract：: Generating and identifying transformants is essential for many studies of gene function. In Arabidopsis thaliana, a revolutionary protocol termed floral dip is now the most widely used transformation method. Although robust, it involves a number of relatively time-consuming and laborious steps, including manipulating...

journal_title：Plant methods

authors： Davis AM,Hall A,Millar AJ,Darrah C,Davis SJ

更新日期：2009-02-27 00:00:00

abstract：Background:Measurements of rind and culm thickness and stem radius/diameter are important to biomechanical, ecological and physiological plant studies. However, many methods of measuring rind thickness and diameter are labor intensive and induce plant fatality. A novel rind puncture methodology for obtaining measuremen...

journal_title：Plant methods

authors： Seegmiller WH,Graves J,Robertson DJ

更新日期：2020-04-01 00:00:00

abstract：BACKGROUND:Kinome profiling aims at the parallel analysis of kinase activities in a cell. Novel developed arrays containing consensus substrates for kinases are used to assess those kinase activities. The arrays described in this paper were already used to determine kinase activities in mammalian systems, but since sub...

journal_title：Plant methods

authors： Ritsema T,Joore J,van Workum W,Pieterse CM

更新日期：2007-02-12 00:00:00