Real-time, non-destructive and in-field foliage yield and growth rate measurement in perennial ryegrass (Lolium perenne L.).

abstract：BACKGROUND:Pollen tubes extend rapidly when pollen grains are incubated in defined media. Tube extension requires many critical functions of plant cells including molecular signaling, cytoskeleton remodeling, secretion, and cell wall synthesis. Consequently, pollen tube growth has been established as a way to conduct p...

journal_title：Plant methods

authors： Hartman E,Levy C,Kern DM,Johnson MA,Basu A

更新日期：2014-01-23 00:00:00

abstract：BACKGROUND:Kinome profiling aims at the parallel analysis of kinase activities in a cell. Novel developed arrays containing consensus substrates for kinases are used to assess those kinase activities. The arrays described in this paper were already used to determine kinase activities in mammalian systems, but since sub...

journal_title：Plant methods

authors： Ritsema T,Joore J,van Workum W,Pieterse CM

更新日期：2007-02-12 00:00:00

abstract：Background:Non-contact resonant ultrasound spectroscopy (NC-RUS) has been proven as a reliable technique for the dynamic determination of leaf water status. It has been already tested in more than 50 plant species. In parallel, relative water content (RWC) is highly used in the ecophysiological field to describe the de...

journal_title：Plant methods

authors： Fariñas MD,Jimenez-Carretero D,Sancho-Knapik D,Peguero-Pina JJ,Gil-Pelegrín E,Gómez Álvarez-Arenas T

更新日期：2019-11-07 00:00:00

abstract：Background:Measurements of rind and culm thickness and stem radius/diameter are important to biomechanical, ecological and physiological plant studies. However, many methods of measuring rind thickness and diameter are labor intensive and induce plant fatality. A novel rind puncture methodology for obtaining measuremen...

journal_title：Plant methods

authors： Seegmiller WH,Graves J,Robertson DJ

更新日期：2020-04-01 00:00:00

abstract：BACKGROUND:In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made to characterise such putative plant channels at the functional level have most often relied on electrophysiological analyses in classical expre...

journal_title：Plant methods

authors： Hosy E,Duby G,Véry AA,Costa A,Sentenac H,Thibaud JB

更新日期：2005-12-19 00:00:00

abstract：Background:Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very young plants not grown in soil and not allowing full development of the root system. Results:This s...

journal_title：Plant methods

authors： Chen S,Svane SF,Thorup-Kristensen K

更新日期：2019-12-09 00:00:00

abstract：Background:Plant roots release a variety of organic compounds into the soil which alter the physical, chemical and biological properties of the rhizosphere. Root exudates are technically challenging to measure in soil because roots are difficult to access and exudates can be bound by minerals or consumed by microorgani...

journal_title：Plant methods

authors： Kawasaki A,Okada S,Zhang C,Delhaize E,Mathesius U,Richardson AE,Watt M,Gilliham M,Ryan PR

更新日期：2018-12-20 00:00:00

abstract：BACKGROUND:The inability to genetically transform any fern species has been a major technical barrier to unlocking fern biology. Initial attempts to overcome this limitation were based on transient transformation approaches or achieved very low efficiencies. A highly efficient method of stable transformation was recent...

journal_title：Plant methods

authors： Plackett AR,Rabbinowitsch EH,Langdale JA

更新日期：2015-07-03 00:00:00

abstract：Background:Wheat is one of the most widely grown crop in temperate climates for food and animal feed. In order to meet the demands of the predicted population increase in an ever-changing climate, wheat production needs to dramatically increase. Spike and grain traits are critical determinants of final yield and grain ...

journal_title：Plant methods

authors： Hughes N,Askew K,Scotson CP,Williams K,Sauze C,Corke F,Doonan JH,Nibau C

更新日期：2017-11-01 00:00:00

abstract：BACKGROUND:We previously developed a high-throughput system called 'Ice-Cap' for growing Arabidopsis seedlings in a 96-well format and rapidly collecting tissue for subsequent DNA extraction and genotyping. While the originally described Ice-Cap method is an effective tool for high-throughput genotyping, one shortcomin...

journal_title：Plant methods

authors： Clark KA,Krysan PJ

更新日期：2007-06-12 00:00:00

abstract：BACKGROUND:The data generated during a course of a biological experiment/study can be sometimes be massive and its management becomes quite critical for the success of the investigation undertaken. The accumulation and analysis of such large datasets often becomes tedious for biologists and lab technicians. Most of the...

journal_title：Plant methods

authors： Vankadavath RN,Hussain AJ,Bodanapu R,Kharshiing E,Basha PO,Gupta S,Sreelakshmi Y,Sharma R

更新日期：2009-12-10 00:00:00

abstract：Background:Plant parasitic weeds belonging to the genus Striga are a major threat for food production in Sub-Saharan Africa and Southeast Asia. The parasite's life cycle starts with the induction of seed germination by host plant-derived signals, followed by parasite attachment, infection, outgrowth, flowering, reprodu...

journal_title：Plant methods

authors： Masteling R,Voorhoeve L,IJsselmuiden J,Dini-Andreote F,de Boer W,Raaijmakers JM

更新日期：2020-05-01 00:00:00

abstract：Background:Lignocellulosic biomass is a complex network of polymers making the cell walls of plants. It represents a feedstock of sustainable resources to be converted into fuels, chemicals and materials. Because of its complex architecture, lignocellulose is a recalcitrant material that necessitates some pretreatments...

journal_title：Plant methods

authors： Terryn C,Paës G,Spriet C

更新日期：2018-08-27 00:00:00

abstract：:Receptor-like protein kinases (RLKs) are a large and important group of plant proteins involved in numerous aspects of development and stress response. Within this family, homo-oligermization of receptors followed by autophosphorylation of the intracellular protein kinase domain appears to be a widespread mechanism to...

journal_title：Plant methods

authors： Taylor I,Seitz K,Bennewitz S,Walker JC

更新日期：2013-06-26 00:00:00

abstract：BACKGROUND:In eukaryotic cells, the proteasome maintains homeostasis by selectively degrading regulatory and misfolded proteins, and in doing so contributes to the amino acid pool. Inhibition of the proteasome in yeast and human cells decreases de novo protein synthesis. However, it is not know if proteasome inhibition...

journal_title：Plant methods

authors： Van Hoewyk D

更新日期：2016-03-16 00:00:00

abstract：Background:Many agronomic traits have been bred into modern wheat varieties, but wheat (Triticum aestivum L.) continues to be vulnerable to heat stress, with high night-time temperature (HNT) stress shown to have large negative impact on yield and quality. Global mean temperature during the day is consistently warming ...

journal_title：Plant methods

authors： Hein NT,Wagner D,Bheemanahalli R,Šebela D,Bustamante C,Chiluwal A,Neilsen ML,Jagadish SVK

更新日期：2019-04-24 00:00:00

abstract：Background:Assessing the impact of the environment on plant performance requires growing plants under controlled environmental conditions. Plant phenotypes are a product of genotype × environment (G × E), and the Enviratron at Iowa State University is a facility for testing under controlled conditions the effects of th...

journal_title：Plant methods

authors： Bao Y,Zarecor S,Shah D,Tuel T,Campbell DA,Chapman AVE,Imberti D,Kiekhaefer D,Imberti H,Lübberstedt T,Yin Y,Nettleton D,Lawrence-Dill CJ,Whitham SA,Tang L,Howell SH

更新日期：2019-10-23 00:00:00

abstract：Background:High throughput non-destructive phenotyping is emerging as a significant approach for phenotyping germplasm and breeding populations for the identification of superior donors, elite lines, and QTLs. Detection and counting of spikes, the grain bearing organs of wheat, is critical for phenomics of a large set ...

journal_title：Plant methods

authors： Misra T,Arora A,Marwaha S,Chinnusamy V,Rao AR,Jain R,Sahoo RN,Ray M,Kumar S,Raju D,Jha RR,Nigam A,Goel S

更新日期：2020-03-18 00:00:00

abstract：:The genomic sequences of many important Triticeae crop species are hard to assemble and analyse due to their large genome sizes, (in part) polyploid genomes and high repeat content. Recently, the draft genomes of barley and bread wheat were reported thanks to cost-efficient and fast NGS technologies. The genome of bar...

journal_title：Plant methods

authors： Spannagl M,Martis MM,Pfeifer M,Nussbaumer T,Mayer KF

更新日期：2013-09-06 00:00:00

abstract：BACKGROUND:Archegoniates (bryophytes, ferns and gymnosperms), such as the moss Physcomitrella patens, possess freely motile sperm cells (spermatozoids) which reach the egg cell via surface water. Although these motile flagellated sperm cells are a traditional botanical subject, they have not been thoroughly analysed in...

journal_title：Plant methods

authors： Horst NA,Reski R

更新日期：2017-05-10 00:00:00

abstract：Background:Roses are widely used as garden ornamental plants and cut flowers. Rosa chinensis cv 'Old Blush' has been used as a model genotype in rose studies due to its contribution to recurrent flowering and tea scent traits of modern roses. The deficiency of efficient genetic transformation systems is a handicap limi...

journal_title：Plant methods

authors： Lu J,Bai M,Ren H,Liu J,Wang C

更新日期：2017-12-22 00:00:00

abstract：Background:Monitoring the timing of seedling emergence and early development via high-throughput phenotyping with computer vision is a challenging topic of high interest in plant science. While most studies focus on the measurements of leaf area index or detection of specific events such as emergence, little attention ...

journal_title：Plant methods

authors： Samiei S,Rasti P,Ly Vu J,Buitink J,Rousseau D

更新日期：2020-07-30 00:00:00

abstract：BACKGROUND:Plant metabolites are commonly functionally classified, as defense- or growth-related phytohormones, primary and specialized metabolites, and so forth. Analytical procedures for the quantifications of these metabolites are challenging because the metabolites can vary over several orders of magnitude in conce...

journal_title：Plant methods

authors： Schäfer M,Brütting C,Baldwin IT,Kallenbach M

更新日期：2016-05-26 00:00:00

abstract：Motivation:In 2005, researchers from the French National Research Institute for Agriculture, Food and Environment (Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, INRAE) started a collaboration with the French farmers' seed network Réseau Semences Paysannes (RSP) on bread wheat par...

journal_title：Plant methods

authors： De Oliveira Y,Burlot L,Dawson JC,Goldringer I,Madi D,Rivière P,Steinbach D,van Frank G,Thomas M

更新日期：2020-07-23 00:00:00

abstract：: Large-scale genetic screens in Arabidopsis are a powerful approach for molecular dissection of complex signaling networks. However, map-based cloning can be time-consuming or even hampered due to low chromosomal recombination. Current strategies using next generation sequencing for molecular identification of mutatio...

journal_title：Plant methods

authors： Liu KH,McCormack M,Sheen J

更新日期：2012-03-30 00:00:00

abstract：BACKGROUND:Microsatellites are popular molecular markers in many plant species due to their stable and highly polymorphic nature. A number of analysis methods have been described but analyses of these markers are typically performed on cumbersome polyacrylamide gels or more conveniently by capillary electrophoresis on ...

journal_title：Plant methods

authors： Mackay JF,Wright CD,Bonfiglioli RG

更新日期：2008-05-19 00:00:00

abstract：:Terahertz technology is still an evolving research field that attracts scientists with very different backgrounds working on a wide range of subjects. In the past two decades, it has been demonstrated that terahertz technology can provide a non-invasive tool for measuring and monitoring the water content of leaves and...

journal_title：Plant methods

authors： Gente R,Koch M

更新日期：2015-03-06 00:00:00

abstract：BACKGROUND:Artificial chromosomes (ACs) are a promising next-generation vector for genetic engineering. The most common methods for developing AC constructs are to clone and combine centromeric DNA and telomeric DNA fragments into a single large DNA construct. The AC constructs developed from such methods will contain ...

journal_title：Plant methods

authors： Lin L,Koo DH,Zhang W,St Peter J,Jiang J

更新日期：2011-04-15 00:00:00

abstract：BACKGROUND:Mitochondria play critical roles in plant growth, development and stress tolerance. Numerous researchers have carried out studies on the plant mitochondrial genome structure, mitochondrial metabolism and nuclear-cytoplasmic interactions. However, classical plant mitochondria extraction methods are time-consu...

journal_title：Plant methods

authors： Xu Y,Li X,Huang J,Peng L,Luo D,Zhang Q,Dan Z,Xiao H,Yang F,Hu J

更新日期：2020-11-04 00:00:00

abstract：BACKGROUND:Despite increasing demand, imaging the internal structure of plant organs or tissues without the use of transgenic lines expressing fluorescent proteins remains a challenge. Techniques such as magnetic resonance imaging, optical projection tomography or X-ray absorption tomography have been used with various...

journal_title：Plant methods

authors： Rousseau D,Widiez T,Di Tommaso S,Rositi H,Adrien J,Maire E,Langer M,Olivier C,Peyrin F,Rogowsky P

更新日期：2015-12-18 00:00:00